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Determination and survey of tetracyclines residue in honey by high performance liquid chromatography (액체크로마토그래피를 이용한 벌꿀 중 테트라싸이클린계 항생물질의 정량분석 및 잔류조사)

  • Lee Sung-Mo;Park Eun-Jeong;Hong Jee-Young;Kim Jung-Im;Lee Jung-Goo;Hwang Hyun-Soon;Kim Yong-Hee
    • Korean Journal of Veterinary Service
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    • v.28 no.3
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    • pp.203-213
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    • 2005
  • Oxytetracycline, tetracycline, chlortetracycline and doxycycline in honey were separated by solid phase extraction (SPE) and determined with high performance liquid chromatography (HPLC) with UV/Visible detector. Analysis was carried out using following conditions: XTerra $C_8$ column $(3.9\times150mm\;i.d. 5{\mu}m)$, mobile phase composed of 0.01M oxalic acid : methanol : acetonitrile (820 : 80 : 100, v/v/v), isocratic pump at a flow rate of 0.9 ml/min. and $50{\mu}l$ of injection volume, UV/Visible detector with wavelength of 360nm. The calibration curves of four tetracyclines showed linearity $(\gamma^2>0.999)$ at concentration range of $100\~1,000 ng/ml$. The recoveries in fortified honey represented more than $70\%$ with low coefficient of variation $(<10\%)$ for concentration range of four tetracyclines. The detection limits for oxytetracycline, tetracycline, chlortetracycline and doxycycline were 13.8, 14.6, 26.2 and 24.9ng/g in acacia honey. respectively. We also monitored tetracyclines residue in domestic honey [n : 38, acacia (20), wild flower (18) ] and foreign honey [n=22, legally distributed (13), illegally distributed (9)] using modified Charm II screening and HPLC confirmation methods. Seven of the 60 samples $(11.7\%)$ were suspect positive using modified Charm II screening test. Chlortetracycline residue was found in one foreign honey (illegally distributed) tested at concentrations of 0.22 ppm. Conclusively, for more effective control of tetracyclines used in beekeeping should be further survey for residues in honey and also national guidelines (maximum residue limit : MRL) and methods should be obligatory.

Development of Selection Model of Subway Station Influence Area (SIA) in New town using Categorical and Regression Tree (CART) (CART분석을 이용한 신도시지역의 지하철 역세권 설정에 관한 연구)

  • Kim, Tae-Ho;Lee, Yong- Taeck;Hwang, E-Pyo;Won, Jai-Mu
    • Journal of the Korean Society for Railway
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    • v.11 no.3
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    • pp.216-224
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    • 2008
  • In general, based on criteria of subway law, radius 500m from subway station is defined as SIA(Subway Station Influence Area). Therefore, in this paper, selection models of SIA are developed to identify appropriate SIA for recently developed 4 new towns based based on CART analysis. As a result, following outputs are obtained; (1) walking distance from subway station is the most influential factor to define SIA (2) SIAs vary with new towns (i.e., bundang city: 856m, ilsan sanbon city 508m, pyungchon city 495m), and (3) walking distance from subway station is influential to land price of SIA. In addition, bundang and pyungchon new town are more affected in land price and walking distance. Therefore, it is desirable for current definition of SIA (radius 500m from subway station) to reflect characteristics of land use and walking distance in the new towns.

A Study on the Inducement Distance of Senior-Friendly Park and Evaluation of Green Service Area - Focused on the Pedestrian Aspect - (보행적 측면에서 노인친화형 공원의 유치거리 도출 및 녹지서비스 지역 평가 - 보행자 측면 중심으로 -)

  • Cho, Hyunju;Lee, Soonju
    • Journal of the Korean Institute of Landscape Architecture
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    • v.47 no.1
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    • pp.1-9
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    • 2019
  • The objective of this study was to derive the served distance of the senior-friendly park considering physical changes, which were estimated through a comparison experiment at an actual target site. The time to walk 500m was examined because 500m is considered the served distance of a neighborhood park and as a standard set by the city. The mean walking time of the control group (younger than 65 years) was 536 seconds, while that of the treatment group (older than 65 years) was 889 seconds, which was approximately 1.7 times longer than the control group. The results of this study also showed that the walking time of females was longer than that of males when they were older than 65 years old. The walking velocities of the control group and the treatment groups were also calculated using the mean walking time. The weight estimated by a proportional formula was 0.6. When it was applied to 500m, which is the served distance of a neighborhood park, the served distance of the senior-friendly park was estimated as 300m. Lastly, the green service excluded area was quantified by applying the 300m, the served distance of a senior-friendly park, to the Jung-gu, Daegu, which had the highest elderly population in the Daegu Metropolitan City. The results of this calculation showed that the area served by a city park was $2,425,747m^2$ and the area excluded from the city park service was $4,627,700m^2$ for senior citizens. The results also showed that, in terms of the distributive equity, the administrative districts received unequal am ounts of urban green area services.

Distribution Characteristics of the Concentration of Ambient PM-10 and PM-2.5 in Daegu Area (대구지역 대기 중 PM-10과 PM-2.5의 농도분포 특성)

  • Do, Hwa-Seok;Choi, Su-Jin;Park, Min-Sook;Lim, Jong-Ki;Kwon, Jong-Dae;Kim, Eun-Kyung;Song, Hee-Bong
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.1
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    • pp.20-28
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    • 2014
  • The three air quality monitoring sites, analysed simultaneously PM-10 and PM-2.5, ie. Ihyeondong in industrial area, Manchondong in residential area, Pyeongnidong in streetside, among 13 air quality monitoring sites in Daegu area, were investigated the concentration distribution characteristics of PM-2.5 and PM-10 in the last 2 years (2011~2012). PM-10 concentrations exceeded annual average reference value ($50{\mu}g/m^3$) in Ihyeondong ($52.5{\mu}g/m^3$) and Pyeongnidong ($60.9{\mu}g/m^3$) but satisfied in Manchondong ($44.9{\mu}g/m^3$). All PM-2.5 concentrations exceeded EPA annual standard value of the United States ($15{\mu}g/m^3$) in three points, but also exceeded new control annual standard value ($25{\mu}g/m^3$) coming into effect in 2015. Seasonal concentration of PM-10 appeared the order of spring > winter > fall > summer, and in the case of PM-2.5, the order was winter > spring > fall > summer. Monthly concentrations of PM-10 and PM-2.5 were highest in February and lowest in September. Diurnal concentrations of PM-10 and PM-2.5 increased from 7:00 AM, and recorded the highest concentration between 10:00 AM and 11:00 AM. And after 6:00 PM it lowered continuously and tended to show fixed concentrations from evening until early morning. In addition, the concentration of fine particles during the week was higher than the weekend. The fluctuation in industrial area was larger than the residential area. At the PM-2.5/PM-10 ratio, summer was generally high, spring was the lowest. And, when yellow sand occurred, it was 0.32 to 0.42. It was very low compared to 0.54 to 0.64 during non-yellow sand times. This paper for the state and the characteristics of Daegu' fine particles (PM-10, PM-2.5) will be valuable to future researches of fine particles and air pollution management.

A Study on Public Nuisance in Kwangju City (Survey on Air Pollution and Noise Level) (공해(公害)에 관(關)한 조사연구(調査硏究) -광주시(光州市)의 대기오염(大氣汚染) 및 소음(騷音)에 관(關)한 조사연구(調査硏究)-)

  • Chung, Yo-Han;Kim, Kil-Wng;Moon, Jae-Kyu;Jhoo, Heung-Kyu
    • Journal of Preventive Medicine and Public Health
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    • v.5 no.1
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    • pp.1-7
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    • 1972
  • During the period from June 1st 1971 to November 30 th 1971, studies on air pollution were made in Kwangju city. The city was divided into 6 areas; the downtown area, the semi-downtown area, the heavy traffic area, the commercial area, the residential area, the park area, 13 surveying sites were selected each representing the characteristics of the area. The Measurement methods which were used are described below. Sulfur oxides were measured by $PbO_2$ cylinder method, sulfur dioxides ($SO_2$) and carbon monoxide (CO) by the MSA & Kitakwa detector, dustfall by the Deposit gauge method, and the noise levels by the Kanomax soundlevel meter. The results obtained are as follows: 1. The mean value of sulfur oxides in Kwangju city was $1.16mg\;SO_3/day/100cm^2\;PbO_2$, ranging from $0.45mg\;SO_3/day/100cm^2\;PbO_2$ to $3.10mg\;SO_3/day/100cm^2\;PbO_2$. 2. The mean values of sulfur oxides according to its specific area in the city were $1.45mg\;SO_3/day/100cm^2\;PbO_2$ in heavy traffic area, 1.36 in downtown area, 1.23 in semi-downtown area, 1.11 in commercial area, 0.96 in residential area, and 1.07 in park area, respectively. 3. The average concentration of sulfur dioxide was 0.063 ppm from 2 to 5 P.M in Kwangju city. 4. The average concentrations of sulfur dioxides according to its specific area, from 2 to 5 P.M, in the city were 0.084 ppm in heavy traffic area & downtown area, 0.067 in commercial area, 0.053 in semi-downtown area, 0.052 in residential area, and 0.036 in park area. 5. The average concentration of carbon monoxide was 22.3 ppm from 2 to 5 P.M, in Kwangju city. 6. The average concentrations of carbon monoxide according to its specific area, from 2 to 5 P.M, in the city were 27.0 ppm in downtown area, 26.3 in semi-downtown area, 23.0 in heavy traffic area, 21.7 in commercial area, 20.0 in residential arera, and 17.6 in park area. 7. The mean value of dusifall in Kwangju city was $29.28ton/km^2/month$, ranging from $9.85ton/km^2/month$ to $66.34ton/km^2/month$. 8. The mean values of dustfall according to its specific area in the city were $50.37ton/km^2/month$ in semi-downtown area, 42.76 in heavy traffic area, 34.67 in downtown area, 17.77 in commercial area, 14.40 in park area, and 14.76 in residential area. 9. The mean values of the soluble dust in Kwangju city was $10.23ton/Km^2/month$ and that of the insoluble dust was $19.05ton/Km^2/month$. 10. The mean value of noise level in Kwangju city was 62 phon, ranging from 37 phon to 88 phon. 11. The mean values of noise level according to its specific area in the city were 76 phon in heavy traffic area, 67 in semi-downtown area, 64 in downtown area, 59 in commercial area, 52 in part area, and 50 in residential area.

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Design and Implementation of 5G City Service Data Collecting and Sharing System (5G 기반의 스마트시티 서비스 데이터 수집 및 공유 시스템 설계 및 구현)

  • Jang, Sooyoung;Han, Mikyong
    • Annual Conference of KIPS
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    • 2019.10a
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    • pp.21-23
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    • 2019
  • 5G 기반의 스마트시티 서비스 데이터 수집 및 공유 시스템은 5G 융합서비스 플랫폼의 시스템컴포넌트로 설계 및 개발된 시스템이다. 본 시스템은 크게 데이터 수집 서브시스템, 데이터베이스 서브시스템, 및 데이터 공유 서브시스템 세 서브시스템으로 이루어져 있으며, 범용성, 확장성, 유연성 및 개방성에 중점을 두고 설계 및 개발되었다.

Egypt's Science and Technology Parks Outlook : A Focus on SRTACity (City for Scientific Research and Technology Applications)

  • Abdel-Fattah, Yasser R.;Kashyout, Abdel-Hady B.;Sheta, Walaa M.
    • World Technopolis Review
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    • v.2 no.2
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    • pp.96-108
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    • 2013
  • Egypt has been known as the light house of science and innovation not only in the Middle East but to the world across ages. Recently, there have been many ups and downs that positioned Egypt in a lower rank that it actually deserves according to its long history. This review entitles the current condition of science, technology and innovation in Egypt and the consequent setting up of best practices of science and technology parks (STPs) experiences. Egypt's science, technology and innovation (STI) system is highly centralized and dominated by the public sector, with R&D happening mostly in state-run universities and research centers supervised by the Ministry of Higher Education and Ministry of Scientific Research. R&D indicators state that Egypt ranking is 40th worldwide for the published articles (around 10,000 papers in 2011), while the numbers of issued patents (350 local and 50 international in 2011) is still far beyond expected. STPs in Egypt are addressed in this review by three examples; smart village in Cairo, Investment zone in Borg El-Arab City and Technology Valley in Ismailia. The three models are discussed in details and a suggested road map for developing more STPs is estimated.

Assessment of In vitro Antioxidant, Antidiabetic and Cytotoxic Activities of Sphaeranthus africanus Extracts

  • Tran Thi Huyen;Julien Dujardin;Nguyen Thi Thu Huong;Chung Thi My Duyen;Nguyen Hoang Minh;Ha Quang Thanh;Dao Tran Mong;Ly Hai Trieu;Nguyen Mai Truc Tien;Mai Thanh Chung;Nguyen Nhat Minh;Nguyen Thi Ngoc Dan;Huynh Loi
    • Natural Product Sciences
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    • v.29 no.2
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    • pp.98-103
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    • 2023
  • Sphaeranthus africanus is commonly used as a traditional remedy for sore throats and pain treatment in Vietnam. The aerial parts have been studied for its anti-inflammatory and anti-proliferative properties. However, the antioxidant and antidiabetic potential of the plant has not been explored. In this work, hydrophilic extracts of the plant's aerial parts were prepared in order to investigate its antioxidant and anti-diabetic properties. Also, the cytotoxicity of the root was evaluated and compared to that of the aerial parts. All of the extracts inhibited lipid peroxidation with IC50 values ranging from 2.05 to 3.56 ㎍/mL, indicating substantial antioxidant activity. At an IC50 value of 4.80 ㎍/mL, the 50% ethanol extract exhibited the most potent inhibition of α-glucosidase. The cytotoxic activity of root extracts is 2 to 5-fold less than that of the aerial parts. Nevertheless, dichloromethane and ethyl acetate extracts of the root demonstrated a selective effect on leukemia cells, with no harm towards the normal HEK-293 cell line. This work provides a scientific support for the antioxidant and antidiabetic activity of the plant. Hence, it may find a promising material for the development of novel antioxidant and antidiabetic agents. More research can be conducted on the phytochemistry and anticancer activities of the plant's root.

Diffuse-Type Histology Is Prognostic for All Siewert Types of Gastroesophageal Adenocarcinoma

  • Kelly M Mahuron;Kevin M Sullivan;Matthew C Hernandez;Yi-Jen Chen;Joseph Chao;Laleh G Melstrom;I. Benjamin Paz;Jae Yul Kim;Rifat Mannan;James L. Lin;Yuman Fong;Yanghee Woo
    • Journal of Gastric Cancer
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    • v.24 no.3
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    • pp.267-279
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    • 2024
  • Purpose: The optimal treatment for gastroesophageal junction adenocarcinoma (GEJA) remains controversial. We evaluated the treatment patterns and outcomes of patients with locally advanced GEJA according to the histological type. Materials and Methods: We conducted a single-institution retrospective cohort study of patients with locally advanced GEJA who underwent curative-intent surgical resection between 2010 and 2020. Perioperative therapies as well as clinicopathologic, surgical, and survival data were collected. The results of endoscopy and histopathological examinations were assessed for Siewert and Lauren classifications. Results: Among the 58 patients included in this study, 44 (76%) were clinical stage III, and all received neoadjuvant therapy (72% chemoradiation, 41% chemotherapy, 14% both chemoradiation and chemotherapy). Tumor locations were evenly distributed by Siewert Classification (33% Siewert-I, 40% Siewert-II, and 28% Siewert-III). Esophagogastrectomy (EG) was performed for 47 (81%) patients and total gastrectomy (TG) for 11 (19%) patients. All TG patients received D2 lymphadenectomy compared to 10 (21%) EG patients. Histopathological examination showed the presence of 64% intestinal-type and 36% diffuse-type histology. The frequencies of diffuse-type histology were similar among Siewert groups (37% Siewert-I, 36% Siewert-II, and 33% Siewert-III). Regardless of Siewert type and compared to intestinal-type, diffuse histology was associated with increased intraabdominal recurrence rates (P=0.03) and decreased overall survival (hazard ratio, 2.33; P=0.02). With a median follow-up of 31.2 months, 29 (50%) patients had a recurrence, and the median overall survival was 50.5 months. Conclusions: Present in equal proportions among Siewert types of esophageal and gastric cancer, a diffuse-type histology was associated with high intraabdominal recurrence rates and poor survival. Histopathological evaluation should be considered in addition to anatomic location in the determination of multimodal GEJA treatment strategies.

A Study on the Method to Predict Underground Temperature in Gumi City (구미지역의 지중온도예측 방법에 관한 연구)

  • 정수일
    • Journal of the Korean housing association
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    • v.13 no.4
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    • pp.27-33
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    • 2002
  • In Gumi area, the heating and cooling loads for underground building could have not been correctly evaluated since there were no systems accurate underground temperature. For solving this problem two ways of predicting the underground temperature were propose. Firstly, it is to estimate the underground temperature of Gumi area by averaging out the underground temperature of the areas around Gumi city. However, the underground temperature data of the areas around Gumi city was only limited to 0.5m and 1.0m under the ground. Secondly, it is to calculate the underground temperature of Gumi area by using a periodic equation with variable about underground properties. Among these methods, the method of the average date was more correct, but the method of the variable date was more available.