Bang, Keuk Soo;Chang, Young Nam;Jin, Jong Sik;Park, Sang A;Lim, Jae Soo;Park, Jeong Sub;Kim, Jong Sung;Lee, Jeong Ho
Korean Journal of Plant Resources
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v.28
no.1
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pp.126-134
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2015
Glycyrrhiza uralensis Fischer is one of the most commonly used herbs. Recently, the stem and leave of the plant have been interested in physiological activities because the aerial parts have been thrown away. Finding out cultivation method of Glycyrrhiza uralensis Fischer to improve chemical ingredients and biological activities has been tried these days. In this study, different wavelengths of light emitting diode (LED) were used for a cultivation of Glycyrrhiza uralensis Fischer. Antioxidant activities and inhibitory effect on mutagenecity of samples were evaluated. The stem and leave cultivated under blue light (BL-0) showed the strongest antioxidant activities of $3.02{\pm}0.13{\mu}g/ml$ ($EC_{50}$) and $2.18{\pm}0.18{\mu}g/ml$ ($EC_{50}$) in DPPH and ABTS radical scavenging test, respectively. Total phenolic content of BL-0 was $2.93{\pm}0.11g/100g$, the highest value between cultivation conditions. However, antioxidant activities of the stem and leave cultivated under red light were the weakest between samples. All of the stem and leave used in this study showed inhibitory effect on mutagenecity of 1-nitropyrene. BL-0 showed stronger inhibitory effects on mutagenicity of Trp-P-1, Trp-P-2, and AFB1 than samples cultivated under other conditions. Only on mutagenecity of 2-aminoanthracene, the stem and leave cultivated at 1 m apart from red light (RL-1) showed the strongest inhibitory effect. These results indicate that blue LED might be the most effective condition for improvement of physiological activities for the aerial parts of Glycyrrhiza uralensis Fischer in cultivation. The components were identified with GC/MS. Cytidine was detected only in RL-1 at 25 min of retention time and 2-bromotrimethylene glycol was detected only in BL-0 at 37 min.
Kim, Do-Ik;Choi, Duck-Soo;Ko, Suk-Ju;Kang, Beom-Ryong;Park, Chang-Gyu;Kim, Seon-Gon;Park, Jong-Dae;Kim, Sang-Soo
Korean journal of applied entomology
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v.51
no.4
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pp.431-438
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2012
The developmental time of the nymphs of Myzus persicae was studied in the laboratory (six constant temperatures from 15 to $30^{\circ}C$ with 50~60% RH, and a photoperiod of 14L:10D) and in a green-pepper plastic house. Mortality of M. persicae in laboratory was high in the first(6.7~13.3%) and second instar nymphs(6.7%) at low temperatures and high in the third (17.8%) and fourth instar nymphs(17.8%) at high temperatures. Mortality was 66.7% at $33^{\circ}C$ in laboratory and $26.7^{\circ}C$ in plastic house. The total developmental time was the longest at $14.6^{\circ}C$ (14.4 days) and shortest at $26.7^{\circ}C$ (6.0 days) in plastic house. The lower threshold temperature of the total nymphal stage was $3.0^{\circ}C$ in laboratory. The thermal constant required for nymphal stage was 111.1DD. The relationship between developmental rate and temperature was fitted nonlinear model by Logan-6 which has the lowest value on Akaike information criterion (AIC) and Bayesian information criterion (BIC). The distribution of completion of each developmental stage was well described by the 3-parameter Weibull function ($r^2=0.95{\sim}0.97$). This model accurately described the predicted and observed occurrences. Thus the model is considered to be good for use in predicting the optimal spray time for Myzus persicae.
To investigate the effect of tillage methods on the silage barely growth and the soil environment in paddy field, liquid pig manure(LPM) was applied after harvesting rice at Jisan series soil for 2 years. Five plots, a LPM applied rate as N%; 0, 100, 150, 200(basal dressing) and 100(basal dressing)+50(additional fertilizer) were divided by tillage methods; non-tillage, non-tillage+rice straw and rotary tillage method. Emission amounts of $NH_3$ gas highly decreased in the rotary tillage and the non-tillage+rice straw plot compared to non-tillage plot. The contents of soil organic matter and exchangeable cation were increased in the applied LPM plot. $NH_4-N$ and $NO_3-N$ contents in soil were the highest in the non-tillage+rice straw plot and followed by the rotary tillage and highly decreased along with the growth of plant. Run-off rate of mineral components were higher in order of the rotary tillage plot£æthe non-tillage plot£æthe non-tillage+rice straw plot and then leached to $SO_4$, $NO_3-N$, K plentifully. The yield of silage barley in dry weight was higher in order of the non-tillage+rice straw plot>the rotary tillage plot>the non-tillage plot. To estimate the feed value of silage barley, crude protein, acid detergent fiber(ADF) and neutral detergent fiber(NDF) contents were analyzed. Crude protein and ADF contents were the highest at rotary tillage N150% plot as 9.7 and 29.4%, respectively. NDF contents was the highest at non-tillage+rice straw N150% plot as 56.7%. In conclusion, we recommend not to incinerate rice straw and to apply LPM at non-tillage status in cultivating the silage barley. This may prevent water pollution and increase barley yields.
We have observed dense core around young stellar objects, DR21, S140, Orion-KL, and L1551 using four millimeter-wave transitions of $HC_3N\;J$=4-3, J=5-4, J=10-9, and J=12-11. The spatial distribution of $HC_3N$ emission closely resembles the morphology of the previous CS observations that trace high density gas. These observations reveal the existence of $HC_3N$ dense cores around central IR source, elliptical in shape and almost perpendicular to the CO bipolar outflow axis. Small differences can be explained by that $HC_3N$ molecular line is more optically thin and is seen to be more detailed structure in the neighborhood of central IR sources. In S140 and Orion-KL, massive(${\sim}10\;M_{\odot}$), slowly rotating dense cores lie near at the central IR sources of bipolar outflows. The velocity channel maps of DR21 show that the bipolar outflow gas may have a correlation with the dense core of DR21. We analyzed intensities of the four lines to derive physical conditions in dense core from two methods, LTE and LVG. The column density of $HC_3N$, $N(HC_3N)$, between LTE and LVG calculations agree well with each other. The abundances of $HC_3N$ in each observing source have been estimated using the average values of $n(H_2)$ and $N(HC_3N)$ and assuming the size of dense core. The fractional $HC_3N$ abundances in massive dense cores of DR21, S140, and Orion-KL have a range of $(2-7){\times}10^{-10}$, while that of low mass dense core, L1551, has one order of magnitude greater value of $2{\times}10^{-9}$. This should be considered good agreement with the result by Morris et al.(1976). It may be considered that dense cores of DR21, S140, and Orion-KL may have almost same stage of chemical evolution, and their abundances have a small values relative to that of L1551. The column density $N(HC_3N)$ decreases with increasing distance from the densest part of the cloud, the central infrared source, and have the relation of $N(HC_3N){\varpropto}R^{\alpha}$, where a has a range of 0.65 to 0.89. The values of $n(H_2)$ are not varied with increasing distance from the dense core, and have almost same values. Therefore, it is considered that the dense cores in these regions probably consist of dense clumps in diffuse molecular gas medium, and $n(H_2)$ of each clump is ${\sim}10^5\;cm^{-3}$. Levels in the $T_{ex}$ increases with $n(H_2)$. It is considered that the $HC_3N$ dense cores are not completely thermalized. We examine the relationships between the luminosity of central infrared sources versus mass of the dense cores, and the luminosity of central infrared sources versus molecular hydrogen column density. Luminosities of the central IR sources show good correlation with mass and hydrogen column density of the dense core. Same has been found from CS observations. However, mass and size derived from $HC_3N$ observations are one order of magnitude smaller than those from CS. It can be interpreted that we see more central part of the cloud cores in $NC_3N$ lines than CS lines.
Journal of the korean academy of Pediatric Dentistry
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v.31
no.4
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pp.555-563
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2004
The pH of beverages is known to be low and have, therefore, been implicated in the increasing incidence of erosion. Erosion is believed to be the predominant cause of teeth wear in children and young adults, although there will always be a contribution from attrition and abrasion. The aim of the present study was to evaluate the effect of yogurt on the progression of erosive demineralization in human enamel using demineralization model in vitro. In 4 yogurts, available on the market, pH, buffering capacity and the concentrations of calcium, phosphate and fluoride were determined. The buffering effect was determined by titration with NaOH. 50 milliliters of each drink was then titrated with 1M sodium hydroxide, added in 0.5 milliliters increments, until the pH reached about 7. Human deciduous enamel(n=40) samples were divided into four groups and exposed to 80ml of the yogurt for 30,60, 90 and 120min. Enamel surface microhardness(VHN) was examined before and after each exposure. 1. The average PH of fermented milk was 3.77 and this pH value was acidic enough to cause tooth erosion. 2. All of the fermented milks were found to be erosive(p<0.05) 3. The teeth exposed to the fermented milk all showed erosion like lesions and microhardness measurements showed that enamel surface hardness decreased proportionately with increased time of immersion in all tooth specimen groups. 4. After immersion for 30 and 60 minutes, reduction rate of microhardness values was not significantly different between the groups(p>0.05). However, after 90 and 120 minutes, reduction rate of each group was significantly different(p<0.05).
This study was conducted to identify the potential of rape stalk as a raw material for biorefinery process of rape flower. At first, rape stalk (RS) was immersed in distilled water (DW), acetic acid (AA), oxalic acid (OA), sulfuric acid (SA) and sodium hydroxide (SH) solutions, and the content of reducing sugars liberated from immersed RS was analyzed. Glucose, xylose, arabinose and sucrose were detected varying with the immersion type. In particular, 1% AA-immersion of RS for 72 hr was the most effective conditions to liberate glucose from RS. Secondly, the RS residues were used for elementary analysis and fabrication of fuel pellets. In addition to the solution type, concentration of immersion solutions (0%, 1%, 2%) and immersion time (24, 72, 120 hr) were used as experimental factors. The contents of nitrogen, sulfur and chlorine reduced effectively through the immersion of RS in DW, AA and OA solutions. For properties of RS-based pellets, bulk density and higher heating value of RS-based pellets greatly increased with the immersion of RS, and the qualities were much higher than those of the A-grade pellet of the EN standards. Ash content decreased remarkably through the immersion of RS, and was satisfied with the A-grade pellet standard. Durability was negatively affected by the immersion of RS, and did not reached to B-grade of the EN standard. In conclusion, acid immersion of RS can be a pretreatment method for the production of fuel pellet and bioethanol, but use of the immersed RS for the production of high-quality pellets might be restricted due to low durability of immersed-RS pellets. Therefore, further studies, such as investigation of detailed immersion conditions, fabrication of mixed pellets with wooden materials and addition of binders, are needed to resolve the problems.
In this study, total antioxidant properties of extracts from different parts of Lespedeza bicolor were determined using techniques of measuring 1,1-diphenyl-2-picryl hydrazyl/2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)-radical scavenging activity and total phenolic contents. The total antioxidant activities of leaf, stem and root extracts from various solvents (water, 50, 70, 100% ethanol, and hot-water) indicated that 50 and 70% ethanol extracts have high radical scavenging activities and phenolic contents. A systematic approach was used to determine the total antioxidant activity of different solvent fractions of the Lespedeza bicolor extracts, partitioning with chloroform, ethyl acetate, n-butanol, and water, and the ethyl acetate fraction was found to have the strongest antioxidant activity. Antioxidant assay-guided isolation was carried out to isolate potential antioxidant compounds. The ethyl acetate fraction of the leaf extract was subjected to silica gel, LH-20 and RP-18 column chromatography successively, and afforded compound 1, which was identified as eriodictyol by NMR and MS analysis, after which its antioxidant activity was determined.
Journal of the Korean Institute of Landscape Architecture
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v.40
no.3
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pp.22-31
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2012
This study was prompted by the recognition that the tenn "Community design" has recently been used in diverse practical fields without prior discussion about its underpinnings, a potentially problematic state of affairs. Based on these problems, this study studied the special quality about the concept of community design. Community design can be discussed from two perspectives. The first views community design as a design that concerns the community, an inhabited area populated with people who have common interests, at least in part because of geographic proximity to each other. The second sees community design as a movement that started in the 1960s and places a great importance on democratic decision making, communication, and collaboration. This study will focus on the latter. This branch of community design encompasses an advocacy planning approach, in which design professionals represent deprived communities in their resistance against comprehensive redevelopment. This was associated to the wider social protest movements of the mid and late 1960s. In the 1970s, this branch of community design was developed alongside community design centers, which provided local-level technical assistance to the communities on a number of issues, such as design and planning. The discussion about community design started in earnest from the early 1980s. A review of the literature m community design reveals several characteristics. First, community design deals with the relationship between the physical environment and several aspects of a region, including the social and cultural. Second, it involves community participation, which many scholars believe is the core of community design. Specifically, community design has been characterized by increased participation and democratic debate and decision making. The Third is about communication methods. Since the 1960s, diverse methods had been developed to promote communication effectively. Finally, community design must consider the relationship between designers, who typically value aesthetics and efficiency of form, and the needs of the community with which they are working. Indeed, some scholars believe that this relationship is generally contentious, although the designer can also be thought of as the facilitator of the community's needs. As community design practice becomes more prevalent, a review of the foundation of institution and policy and the role of experts is also needed. The community design movement bas been theorized ex post facto through diverse discussion that has sought to ascribe meaning and direction to its practice. In other words, the relationship between this theory and practice is cyclical. Therefore, this study can contribute to the virtuous circle.
Journal of the Korean Society of Clothing and Textiles
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v.15
no.2
s.38
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pp.77-87
/
1991
The purpose of this study were to investigate the bed climate and the physiological responses in sleep on the traditional Korean floor heating system called Ondol. Ondol has been the most widely used heating system in Korea, yet there has been a no systematic studies examined its environmental effects on human body. Experimental room was constructed to match the typical thermal environment of Ondol. (floor surface temperature; $32\~33^{\circ}C$, air temperature; $22.5{\pm}1.0^{\circ}C$, relative humidity; $64.0{\pm}4.0\%$RH, air velocity; 0.25 m/sec.) Three different combinations of bedclothes were chosen for the experiment based on the study results showing that they were the most widely used types in Korea. Type 1 was the combination of a cotton-padded mattress with a cotton-padded Korean style blanket. Type 2 was a cotton-padded mattress with a cotton-guilted Korean style blanket. Type 3 was a cotton-padded matless with a polyacryl blanket. Thermal resistance of each of these combina-tions in the bedclothes was measured using thermal manikin. Two adult female was chosen for the seven hour sleeping experiment which was known to be the average sleeping hours of Korean adult female. The bed climate was measured with the temperature under the mattress, the surface temper-ature of the mattress, and the air temperature and the relative humidity of the space between the mattress and blanket. The skin temperature, rectal temperature of the subjects and the bed climate were measured eight times, one hour before the experiment and every hour during the experiment. The weight loss and the subjective sensation were measured for the each subjects before and after the experiment. The procedure was repeated twice with two subjects and three types of bedclothes, yielding twelve combinations of results. The results were as follows; 1. With the surface temperature of $32\~33^{\circ}C$ of Ondol, air temperature was $22.5{\pm}1.0^{\circ}C$ with $64.0{\pm}4.0\%$ RH. The bed climates were $39.2{\pm}40.8^{\circ}C$ under the mattress, $35.3\~36.2^{\circ}C$ on the mattress, and $26.9\~32.0^{\circ}C$ with $56.0\~71.3\%$ RH between the mattress and the blanket. 2. Mean skim temperature during sleep was 34.2"C with local skin temperature of $34.0\~35.5^{\circ}C$. The skin temperatures of abdomen, thigh, foot were higher than the other parts of the body. 3. The skin temperature of chest, thigh, leg and back varied significantly according to the combinations of bedclothes. With the cotton-padded blanket, the skin temperature was the highest, while with the cotton-guilted blanket showed lowest. 4. Examining the relationship between the mean skin temperature and the local skin temper-ature, the chest temperature showed the highest correlation with the former. Therefore, the chest temperature can be recommended to represent the skin temperature in measuring the bed climate. 5. The subjective bed climates were $39.0\~40.4^{\circ}C$ under the mattress, $35.2\~35.9^{\circ}C$ on the mattress, $29.8\~31.6^{\circ}C$ with $56.8\~68.4\%$ RH between the mattress and blanket. In sum, from this experiment we not only obtained the reliable value of bed climates on Ondol, but also showed that the bed climates and the physiological responses were affected differently according to the materials of bed clothes.
Soybean [$Glycine$$max$ (L.) Merr.] protein is a high quality source for food and feed. But, antinutritional factors in the raw mature soybean are exist. Kunitz trypsin inhibitor (KTI) protein is a main antinutritional factor in soybean seed. Also, P34 protein, referred as $Gly$$m$ Bd 30K, has been identified as a predominant immunodominant allergen. Genetic relationship between KTI protein and P34 protein could be useful in soybean breeding program for the genetic elimination or reduction of these factors. The objective of this study was to determine the independent inheritance or linkage between KTI protein and P34 protein in soybean seed. A total of 479 $F_2$ seeds were obtained from the cross of 07B1 and PI567476 parents. KTI protein and relative amount of P34 protein were analysed from $F_2$ seeds harvested from the F1 plants by using SDS-PAGE and Western blot analysis. The segregation ratios of 3 : 1 for KTI protein (353 KTI protein present : 126 KTI protein absent) and relative amount of P34 protein (363 normal amount of P34 protein : 116 low amount of P34 protein). The segregation ratio of 3 : 1 suggested that KTI protein and relative amount of P34 protein in mature soybean seed were controlled by a single major gene. The segregation ratios of 9 : 3 : 3 : 1 (266 KTI protein present, normal amount of P34 protein: 88 KTI protein present, low amount of P34 protein: 102 KTI protein absent, normal amount of P34 protein: 23 KTI protein absent, low amount of P34 protein) and Chi-square value (${\chi}^2$=3.31, P=0.346) were observed in $F_2$ seeds. This data showed that KTI protein was inherited independently with relative amount of P34 protein in soybean. These results will be helpful in breeding program for selecting the line with lacking KTI protein and reduced amount of P34 protein in soybean.
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