• 생명과학회지
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• 제28권6호
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• pp.639-647
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• 2018

한국의 고급 양식대상 어종인 붉바리(Epinephelus akaara)로부터 새로운 heat shock protein (Hsp) 70을 동정하였다. 붉바리 Hsp70 (RgHsp70)의 cDNA는 RACE (rapid amplification of cDNA ends)법을 사용하였고, RgHsp70 cDNA의 전장은 2,152 bp이고, 5'-terminal untranslated region (UTR)은 105 bp, 3'-terminal UTR은 274 bp, 590개의 아미노산을 암호화하는 open reading frame (ORF)는 1,773 bp였으며, 분자무게(molecular weight)는 64.9 kDa 및 등전위값(isoelectric point, pI)은 5.2였다. 추정되는 아미노산 비교 및 계통발생학적 분석 결과, 다른 어종과 마찬가지로 Hsp70 고유의 signature를 포함하는 것을 비롯하여 높은 유사성을 나타내었으므로 RgHsp70이 Hsp70 family임을 확인할 수 있었다 RgHsp70 mRNA는 간과 두신 조직에서 높은 발현을 보였으며, 48시간 동안 수온별(21, 18, 15 및 $12^{\circ}C$) 노출 후 간 조직에서 대조구인 $21^{\circ}C$보다 $12^{\circ}C$에서 발현이 증가함을 확인하였다. 본 연구에서는, 수온이 하강함에 따라 RgHsp70 mRNA 발현에 주요한 영향을 미치는 것으로 보아, 수온변화에 따른 스트레스로 인해 발현의 변화를 나타내는 주요 스트레스성 단백질임을 확인할 수 있었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제37권2호
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• pp.82-89
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• 2018

This study was carried out to understand phylogenetic relationships of the 30 mulberry cultivars converved in Korea based on the ITS rDNA region, and they were compared to 40 reference sequences from GenBank. The size and the G+C content of the ITS rDNA gene regions from the 30 Korean mulberry cultivars and 40 reference sequences varied from 612-630 bp and 58.19-61.62%, respectively. Based on the results of the comparative phylogenetic analysis of the ITS rDNA regions of the 30 Korean mulberry cultivars and 40 reference sequences, they were divided into three groups (Group 1, 2, and 3) and two subgroups (Group 1A and 1B within Group 1). The sequence lengths of the Korean mulberry cultivar numbers 1-26 and 27-30 were 615 bp and 616 bp, respectively. At 205 bp location of ITS1 rDNA region, the cultivar numbers 1-26 contain the nucleotide thymine but the cultivar numbers 27-30 contain the nucleotide adenine. In addition, the insertion of the nucleotide adenine at 206 bp location was found only in the four Korean mulberry cultivars (numbers 27-30). Based on these sequence information and phylogenetic result, the 30 Korean mulberry cultivars were identified as M. alba and M. australis. This study will contribute to the construction of genetic database constructions and accurate variety identifications for unidentified mulberry varieties in Korea.

• Asian-Australasian Journal of Animal Sciences
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• 제24권7호
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• pp.898-904
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• 2011

The PCR- restriction fragment length polymorphism (RFLP) in and around TM4 of SLC11A1 gene and its association with the incidences of brucellosis in Hariana breed (Bos indicus) and Holstein Friesian crossbred (Bos indicus${\times}$Bos taurus) cattle was examined. A fragment of 954 bp encoding the TM4 was amplified, and RFLP was identified by digestion of the amplicon independently with AluI and TaqI. The amplicon (GenBank Acc. No. AY338470 and AY338471) comprised of a part of exon V (<59 bp) and VII (62>), and entire intron 5 (423 bp), exon VI (71 bp) and intron 6 (339 bp). Digestion with AluI revealed the presence of two alleles viz, A (281, 255, 79 and 51 bp) and B (541, 255, 79 and 51 bp). The frequency of A allele was estimated as 0.80 and 0.73 in Hariana and crossbred cattle, respectively. Due to presence of a polymorphic TaqI site at intron 5, two alleles: T (552 and 402 bp) and Q (231, 321 and 402 bp) were identified. The frequency of T allele was estimated as 0.96 and 0.97, respectively. For association study, on the basis of serological tests and history of abortion, the animals were grouped into "affected" and "non-affected". However, no association could be established with the observed RFLPs.

• 한국버섯학회지
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• 제19권4호
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• pp.285-293
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• 2021

본 연구에서는 Flammulina velutipes var. lupinicola의 laccase 유전자를 동정하고 최적 활성 pH, 온도, 시간을 분석하고 하였다. F. velutipes var. lupinicola 유전체에서 선별된 laccase 유전자 서열을 바탕으로 구리 결합 부위 및 신호 펩타이드 분석을 수행한 결과 5종의 laccase 유전자(g1934, g1937, g2415, g2539, g5858)를 동정하였다. 5종의 선별된 laccase 유전자 크기는 1,488~1,662 bp로 확인되었고, cDNA 염기서열 분석 결과 14~17개의 인트론이 확인되었다. Laccase 유전자의 신호펩타이드로 예측된 절단 부위는 N-말단으로부터 20~34 bp 사이에 위치하는 것으로 확인되었다. F. velutipes var. lupinicola laccase의 활성 특성을 규명하기 위해 분리 정제를 수행하였으며, Zymogram을 수행하여 0.2 M 및 0.3 M NaCl과 1.6 M 및 1.7 M의 ammonium sulfate로 정제된 단백질에서 5개의 laccsae 활성 밴드를 확인하였다. pH, 온도 및 시간별로 분리 정제된 단백질의 최적 활성을 분석한 결과, 반응의 최적 pH는 5.5이고 최적 온도는 40℃로 확인되었다. 따라서 본 연구를 통하여 확인된 F. velutipes var. lupinicola 유전체의 laccase 유전자 구조 및 활성에 대한 특성은 laccase를 이해하는 데 도움이 될 것이며 추가 연구를 통하여 향후 다양한 산업적 활용이 가능할 것으로 사료된다.

• Journal of Plant Biotechnology
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• 제39권3호
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• pp.212-218
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• 2012

Glycosyltransferases are enzymes (EC 2.4) that catalyze the transfer of monosaccharide moieties from activated nucleotide sugar to a glycosyl acceptor molecule which can be a carbohydrate, glycoside, oligosaccharide, or a polysaccharide. In this study, a UDP-glucosyltransferase cDNA was isolated from Brassica rapa using a rapid amplification of cDNA ends (RACE) and subsequently named BrUGT. It has a full-length cDNA of 1,236 bp with 119 bp 5'-untranslated region (UTR), a complete ORF of 834 bp encoding a polypeptide of 277 amino acids (31.19 kDa) and a 3'-UTR of 283 bp. BLASTX analysis hits a catalytic domain of Glycos_transf_1 super family (cl12012) that belongs to the Glycosyltransferases group 1 with tetratricopeptide (TPR) regions located between 165 to 350 bp. Expression analysis showed high mRNA transcripts in pistil, followed by petal, seed and calyx of flower. Moreover, expression analysis of BrUGT in Chinese cabbage seedlings under stresses of cold, salt, PEG, $H_2O_2$, drought and ABA showed elevated mRNA transcript. Furthermore, when BrUGT gene was transformed into rice using pUbi-1 promoter, overexpression was evident among the $T_1$ plants. This study provides insights into the function of BrUGT in plants.

• 한국양식학회지
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• 제20권1호
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• pp.65-72
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• 2007

우리나라 주요 담수어종인 미꾸라지(Misgurnus mizolepis)로 부터 apolipoprotein A-I (apoA-I) cDNA를 분리하고 그 구조, 분자 계통 및 발현 특징을 분석하였다. 미꾸라지 apoA-I cDNA는 254개의 아미노산을 암호화하고 있는 762 bp의 ORF를 포함하고 있었으며 아울러 24 bp의 5'UTR 및 293 bp의 3'UTR(종결 코돈 및 poly A tail 제외)를 갖고 있었다. 미꾸라지 apoA-I은 여타 척추동물 apoA-I과의 다중배열 시 염기서열에서는 많은 차이를 나타내었지만 단백질의 구조적 특징은 높은 상동성을 보였고, 또한 척추동물의 apoA-I들과의 분자계통을 분석한 결과, 종래 알려진 분류학적 위치와 비교적 잘 일치하였다. 미꾸라지 apoA-I mRNA는 RT-PCR 분석을 통해 간 및 뇌 조직에서 분석한 다른 조직보다 유의적으로 높게 발현하는 것으로 나타났고, 특히 간에서 가장 높은 발현을 보였다. 수정시부터 부화 후 14일까지 초기 발생 및 치어에서의 apoA-I mRNA 발현을 조사한 결과 수정 8시간째부터 급격한 발현의 증가가 시작되어 이후 지속적으로 높은 발현 수준을 유지하였다.

• Preventive Nutrition and Food Science
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• 제8권1호
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• pp.61-65
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• 2003

This study was investigated to identify the regulatory mechanism of ACC gene expression by hormones and nutrition. The fragment of ACC promoter I (PI) -220 bp region was recombined to pGL3-Basic vector with luciferase as a reporter gene. The primary hepatocyte from the rat was used to investigate the regulation of ACC PI activity. ACC PI (-220 bp)/luciferase chimeric plasmid was transfected into primary rat hepatocyte by using lipofectin. ACC PI activity was shown by measuring luciferase activity. The addition of insulin, dexamethasone, and triiodothyronine to the culture medium increased the activity of ACC PI by 2.5-, 2.3- and 1.8-fold, respectively. In the presence of 1 $\mu$M dexamethasone, the effects of insulin was amplified about 1.2-fold showing the additional effects of dexamethasone. Moreover the activity of luciferase was increased by insulin, dexamethasone, and triiodothyronine treatment approximately 4-fold. These results indicated that insulin, dexamethasone and thyroid hormone coordinately regulate ACC gene expression via regulation of promoter I activity. On the -220 to ＋21 region of ACC PI, the addition of the glucose to the culture medium increased the activity of ACC PI. With 25 mM glucose, luciferase activity increased by 7-fold. On the other hand, on the -220 bp region, ACC PI activity was not changed by polyunsaturated fatty acids. Therefore, it can be postulated that there are response elements for insulin, triiodothyronine, dexamethasone, and glucose, but not PUFAs on the -220 bp region of ACC PI.

• Experimental and Molecular Medicine
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• 제50권11호
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• pp.11.1-11.12
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• 2018

USP15 has been shown to stabilize transcription factors, to be amplified in many cancers and to mediate cancer cell survival. However, the underlying mechanism by which USP15 regulates multiple myeloma (MM) cell proliferation and apoptosis has not been established. Here, our results showed that USP15 mRNA expression was upregulated in MM patients. USP15 silencing induced MM cell proliferation inhibition, apoptosis, and the expression of nuclear and cytoplasmic NF-${\kappa}Bp65$, while USP15 overexpression exhibited an inverse effect. Moreover, in vivo experiments indicated that USP15 silencing inhibited MM tumor growth and NF-${\kappa}Bp65$ expression. PDTC treatment significantly inhibited USP15 overexpression-induced cell proliferation, apoptosis inhibition, and NF-${\kappa}Bp65$ expression. USP15 overexpression promoted NF-${\kappa}Bp65$ expression through inhibition of its ubiquitination, whereas NF-${\kappa}Bp65$ promoted USP15 expression as a positive regulator. Taken together, the USP15-NF-${\kappa}Bp65$ loop is involved in MM tumorigenesis and may be a potential therapeutic target for MM.

• Fisheries and Aquatic Sciences
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• 제13권3호
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• pp.224-230
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• 2010

Growth hormone (GH) is known as one of the main osmoregulators in euryhaline teleosts during seawater (SW) adaptation. Many of the physiological actions of GH are mediated through insulin-like growth factor-I (IGF-I), and the GH/IGF-I axis is associated with osmoregulation of fish during SW acclimation. However, little information is available on the response of fish IGF-II to hyperosmotic stress. Here we present the first cloned IGF-I and IGF-II cDNAs of marine medaka, Oryzias dancena, and an analysis of the molecular characteristics of the genes. The marine medaka IGF-I cDNA is 1,340 bp long with a 257-bp 5' untranslated region (UTR), a 528 bp 3' UTR, and a 555-bp open reading frame (ORF) encoding a propeptide of 184 amino acid (aa) residues. The full-length marine medaka IGF-II cDNA consists of a 639 bp ORF encoding 212 aa, a 109 bp 5' UTR, and a 416 bp 3' UTR. Homology comparison of the deduced aa sequences with other IGF-Is and IGF-IIs showed that these genes in marine medaka shared high structural homology with orthologs from other teleost as well as mammalian species, suggesting high conservation of IGFs throughout vertebrates. The IGF-I mRNA level increased following transfer of marine medaka from freshwater (FW) to SW, and the expression level was higher than that of the control group, which was maintained in FW. This significantly elevated IGF-I level was maintained throughout the experiment (14 days), suggesting that in marine medaka, IGF-I is deeply involved in the adaptation to abrupt salinity change. In contrast to IGF-I, the increased level of marine medaka IGF-II mRNA was only maintained for a short period, and quickly returned a level similar to that of the control group, suggesting that marine medaka IGF-II might be a gene that responds to acute stress or one that produces a supplemental protein to assist with the osmoregulatory function of IGF-I during an early phase of salinity change.

• 대한지리학회지
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• 제32권1호
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• pp.15-30
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• 1997

花粉, 沖積層의 堆積相, 炭素年代 등의 분석자료를 기초로 식생환경, 해면변동 및 홀로세 환경변화를 고찰하고, 각 시기별 古地理圖를 작성하였다. 각 花粉帶 별 環境특성은 다음과 같다. 1) 화분대 I(3.75~5.75m)dms 8,000~4,200년BP 시기로서, 우점식생은 Alnus-EMW로 대표된다. 빠른 해면상승과 해진의 영향으로 매우 습윤하였다. 2) 화분대 II(5.75~6.35m)는 4,200~2,300년BP 시기로서, 해수면 하강 및 地下水面 하강으로 건륙화된 환경하에 Pinus가 증가하고, 포자류와 초본류가 우점하였다. 3) 화분대 III(6.35~6.55m)은 2,300년BP에서 1,800년BP 까지 지속되었으며, 해진의 영향과 인간의 간섭을 모두 반영하는 초본류의 비율이 높은 시기였다. 아분대 IIb와 화분대 III의 경계는 베버의 限界層의 특징을 나타낸다.