• Microbiology and Biotechnology Letters
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• v.9 no.3
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• pp.165-171
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• 1981

The penicillin amidase from Escherichia coli (ATCC 9637) was immobilized by entrappment in gelatin and DEAE-cellulose mixture cross-linked with glutaraldehyde, and the kinetics in a differential column reactor was studied. The optimal operating condition of a differential reactor was reasonably met when the enzyme loading was 1g, and 30 mM substrate solution in 0.1 M phosphate buffer (pH 8.0) was fed at flow rate 4$m\ell$/min and 4$0^{\circ}C$. The optimal pH and temperature were found to be 8.0 and 55$^{\circ}C$, respectively. The Michaelis-Menten constant was 4.8 mM while the maximum velocity was 308 units/g of the immobilized enzyme under the condition of the differential reactor. The effect of substrate inhibition disappeared in the immobilized enzyme preparation. The differential reactor was proved to be good for studying the true kinetics since the pH drop and the external diffusional resistance could be eliminated.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.11
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• pp.2751-2757
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• 2015

To obtain the system requirement specification in the beginning of the precision guidance and control system development, the effectiveness and reliability analysis for the system are necessary. The main purpose of this research is to obtain the system requirement specification by carrying out the effectiveness analysis using the modeling and simulation(M&S) scheme. M&S model is constructed using 6-DOF dynamic model, environment model, guidanc -navigation & control model. Assume that the navigation sensor is consist of inertial navigation sensor(INS) and doppler velocity log(DVL), and the speed and direction of current is environment parameter. The effectiveness analysis is carried out using circular error probability(CEP) and variance analyze scheme. Also, the effectiveness analysis is utilized for cost-performance analysis considering the cost of commercial INS and DVL sensor. This paper shows the high-level INS and the low-level DVL configure a high price-performance integrated navigation system.

• Journal of Korea Port Economic Association
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• v.28 no.1
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• pp.179-201
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• 2012

Since 1993, M&A activities have been frequent in Liner shipping market. This study examines the effect of M&A on stock price reaction for acquiring firms listed on the market. The study covers the period from 1993 to 2009 and uses 61 daily closing prices of the acquiring firms before and after the M&A announcement day and is analyzed through the market-adjusted model in an event study. After calculating short-term performance using abnormal returns(AR) and cumulative average abnormal returns(CAR) before and after 30 days from the day of event, the results on the test show that the firm's values slightly increased through the M&A, but it does not attest to the statistical significance. In addition, this study investigates the AR difference between estimating windows and post-event windows for the 3 cases of each period before and after 30 days, 15 days, and 7 days from the event day to analyse the impact of M&A on the addition of acquiring firm's value. Our findings suggest that the M&A between Liner shipping companies is targeted for the long-term business strategy instead of the instant rise in the value of the firm involved.

• Journal of Life Science
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• v.30 no.2
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• pp.147-155
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• 2020

In this study, the antioxidant and cytotoxic effects and the flavonoid contents of leaf extracts from Stachys sieboldii Miq. and Lycopus lucidus Turcz. were compared. The flavonoid contents of the acetone + methylene chloride (A+M) and methanol (MeOH) extracts of L. lucidus Turcz. leaves were 55.7 and 233.2 mg/g, respectively. In a DPPH assay, A+M and MeOH extracts from L. lucidus Turcz leaves had a greater scavenging effect than those of S. sieboldii Miq. leaves (p<0.05). In an ABTS assay, MeOH extracts from S. sieboldii Miq. and L. lucidus Turcz (0.5 mg/ml concentration) leaves had scavenging effects of 85% and 91%, respectively (p<0.05), suggesting that both of the MeOH extracts had greater scavenging effects than both A+M extracts. In a 120 min ROS production assay, all tested extracts decreased the cellular ROS production induced by H₂O₂ compared to that produced by exposure to the extract-free control. The MeOH extract from L. lucidus Turcz leaves had a greater inhibitory effect on cellular ROS production (p<0.05). Treatment with A+M and MeOH extracts from both S. sieboldii Miq. and L. lucidus Turcz. leaves showed a dose-dependent increased cytotoxicity against the growth of AGS, HT-29 cancer cells, and HT-1080 (p<0.05). Both A+M extracts had a greater inhibitory effect on the growth of all cancer cells than both MeOH extracts. These results suggest that the MeOH extract of L. lucidus Turcz. leaves is effective in scavenging free radicals and inhibiting cellular oxidation, while the A+M extract inhibits proliferation of three types of cancer cell.

• Journal of Applied Biological Chemistry
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• v.60 no.2
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• pp.119-124
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• 2017

The extracted phenolic compounds from Pinus densiflora root were examined biological activities for beauty food. The tyrosinase inhibitory activity which was related to skin-whitening was observed. The tyrosinase inhibitory activity was confirmed to be 92% in ethanol extract at $50{\mu}g/mL$ phenolic. The elastase and collagenase inhibitory activity as anti-wrinkle effect were showed 61 and 78% in ethanol extract at $200{\mu}g/mL$ phenolic, respectively. Astringent effect of ethanol extract was showed to be 82% at $50{\mu}g/mL$ phenolic. Hyaluronidase inhibitory activity of ethanol extract as anti-inflammation effect was confirmed to be 94% of inhibition at $200{\mu}g/mL$ phenolic. These results demonstrated that isolated phenolic compounds from P. densiflora root could be expected to use as a functional cosmetic materials.

• Journal of Convergence for Information Technology
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• v.12 no.3
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• pp.236-243
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• 2022

This study attempted to find the optimum amount of manuka extract ingredient with hair treatments in different manuka extract contents and examine its bleached hair protection effects. For this, the tensile strength and moisture level of the damaged hair were measured. In addition, hair conditions were analyzed, using SEM and FE-SEM. In terms of protection of bleached hair, all experimental groups (M1, M2, M3) were more effective than the control group (no manuka extract added) in tensile strength, moisture level, hair thickness and cuticle conditions. In particular, 'M3 (3% manuka extract)' revealed the largest hair protection effects. The above results confirm that Manuka extract-added hair treatments would be useful in protecting damaged hair as well as scalp as a cosmetic material. It is anticipated that there would be further studies on diverse chemical treatments with a much wider variety of samples.

• Journal of Life Science
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• v.19 no.5
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• pp.633-638
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• 2009

We investigated the inhibitory effects of solvent extracts from dried tuna on the growth of cancer cell lines (HT1080 human fibrosarcoma and HT-29 human colon cancer cells) and $H_2O_2$-induced oxidative stress. Inhibitory effects of acetone with methylene chloride (A+M) and methanol (MeOH) extracts on the growth of HT1080 and HT-29 cancer cells increased in a dose dependent manner (p<0.05). The inhibitory effect was more significant on the growth of HT1080 cells, and A+M extracts had a higher inhibitory effect compared to MeOH extracts. The treatments of hexane, 85% aq. methanol, butanol and water fractions significantly inhibited the growth of both cancer cells (p<0.05). Among the fractions, hexane and 85% aq. methanol fractions showed higher inhibitory effects. In order to determine the protective effect on $H_2O_2$-induced oxidative stress, a DCHF-DA (dichlorodihydrofluorescin diacetate) assay was conducted. All fractions, including crude extracts of dried tuna, appeared to significantly reduce the levels of intracellular reactive oxygen species (ROS) with dose responses (p<0.05). Among the fractions, BuOH and 85% methanol fractions showed a higher protective effect on the production of lipid peroxides. These results indicate that the consumption of tuna may be recommended as a potent functional food for preventing cellular oxidation and cancer.

• The Korean Journal of Pesticide Science
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• v.9 no.4
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• pp.429-436
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• 2005

As Sclerotinia sclerotiorum causing cucumber sclerotinia rot was the fastest in the mycelial growth at $25^{\circ}C$, its pathogenicity was strong at the same temperature among several temperatures. All the isolates of Sclerotinia sclerotiorum showed a strong pathogenicity against cucumber fruits, which was confirmed by a disk assay and a wound assay. A wound assay was superior to a disk assay to develop the assay system for assessing the fungicidal activity of several fungicides against Sclerotinia sclerotiorum. In a disk assay, it was very difficult to assess the fungicidal activity, because the pathogenicity of isolates used in the experiment was very strong. At 500 and $3.0{\mu}g/mL$, the activity of dichloflouanid and the mixture of carbendazim and diethofencarb against cucumber sclerotinia rot was 14.3 and 42.3%, respectively, by using a disk assay. However, at same concentration two fungicides showed the high controlling activity as 100 and 92.5%, through a wound assay in a laboratory. Also, the activity of two fungicides was good against cucumber sclerotinia rot in the greenhouse where cucumber plants were cultivated in the field, showing the control value as 91.1 and 82.9% at 100 and $825{\mu}g/mL$, respectively. All the isolates of Sclerotinia sclerotiorum from cucumber fruits sampled in the polyvinyl house were subjected to monitoring for the resistance to 7 fungicides. The $EC_{50}$ value of 7 fungicides was as follows: fenhexamid; $0.13{\mu}g/mL$, procymidon and iprodione; 0.18 and $0.24{\mu}g/mL$, carbendazim and the mixture of carbendazim and diethofencarb; 0.13과 $0.05{\mu}g/mL$, iminoctadine and dichlofluanid; 1.94 and $8.95{\mu}g/mL$. Ultimately it was not found that resistant isolates of Sclerotinia sclerotiorum were appeared in the field.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.32 no.6B
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• pp.387-397
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• 2012

In this study, an ecological fragmentation improvement effect from different riverine ecobelt width was analyzed. The target river for this study is the Hongcheon river in Kangwondo. This section of 11 km among the Hongchenon river was reported to be seriously fragmented in the previous literatures. The Hongcheon river of 11 km was divided longitudinally into subsections of 1 km. The analysis through map and aerial photograph as well as field surveys were performed in 11 subsections(A-K). Using the fragmentation index, the connectivity fragmentation such as river bank, road, and land use and the ecological functional fragmentation were evaluated. The results showed that I-section has the highest fragmentation rate. The Hongcheon river in the present status without ecobelt was analyzed to has the fragmentation of 2nd rate from the mean fragmentation value of 11-subsections. Also, when the riverine ecobelt of 10 m-50 m was restored, the improvement effect of connectivity fragmentation was relatively small, while for the ecological fragmentation, the improvement effect of 12.4-25.1% was predicted. Also, the ecobelts of 10 m and 30 m were evaluated to have a relatively great improvement effect. Especially, for the ecobelt of 30m width, the fragmentation rate was mitigated form 2nd rate to 3rd rate.

• Journal of the korean academy of Pediatric Dentistry
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• v.39 no.4
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• pp.366-372
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• 2012

The aim of this study was to compare two commercial root canal medicaments, ProRoot MTA (Dentsply Tulsa, USA) and OrthoMTA (Bio MTA, Korea), by assessing the antimicrobial effects on three selected species commonly found in root canals of infected teeth, namely Enterococcus faecalis, Fusobacterium nucleatum and Staphylococcus epidermidis. Colonies of these bacteria were treated with varied concentrations of ProRoot MTA or OrthoMTA over different lengths of time. The results are as follows : 50, 25 and 12.5 mg/mL of ProRoot MTA or OrthoMTA did not completely inhibit the growth of E. faecalis, but a decreased growth rate was evident in comparison to the control (p < 0.05). 50 mg/mL of both materials successfully eliminated F. nucleatum during the first 24 hours. Regrowth of microbes after 24 hours, however, indicated a diminished effect of ProRoot MTA whereas OrthoMTA showed its continuously sustained antimicrobial actions (p < 0.05). 50 mg/mL of ProRoot MTA and 50, 25 mg/mL of OrthoMTA exerted their full antibacterial actions against S. epidermidis during the first 24 hours. Although the regrowth of colony was observed after 24 hours, the rate of growth was significantly decreased, approximating a complete inhibition (p < 0.05). The present study revealed that OrthoMTA, recently developed in Korea, had antimicrobial activity higher than ProRoot MTA.