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http://dx.doi.org/10.5352/JLS.2009.19.5.633

In Vitro Anticancer and Antioxidant Effect of Solvent Extracts from Tuna Dried at Low Temperature Vacuum.  

Jang, Joo-Ri (Division of Marine Environment & Bioscience, Korea Maritime University)
Kim, Kyung-Kun (Division of Marine System Engineering Korea Maritime University)
Mun, Soo-Beom (Division of Marine System Engineering Korea Maritime University)
Lim, Sun-Young (Division of Marine Environment & Bioscience, Korea Maritime University)
Publication Information
Journal of Life Science / v.19, no.5, 2009 , pp. 633-638 More about this Journal
Abstract
We investigated the inhibitory effects of solvent extracts from dried tuna on the growth of cancer cell lines (HT1080 human fibrosarcoma and HT-29 human colon cancer cells) and $H_2O_2$-induced oxidative stress. Inhibitory effects of acetone with methylene chloride (A+M) and methanol (MeOH) extracts on the growth of HT1080 and HT-29 cancer cells increased in a dose dependent manner (p<0.05). The inhibitory effect was more significant on the growth of HT1080 cells, and A+M extracts had a higher inhibitory effect compared to MeOH extracts. The treatments of hexane, 85% aq. methanol, butanol and water fractions significantly inhibited the growth of both cancer cells (p<0.05). Among the fractions, hexane and 85% aq. methanol fractions showed higher inhibitory effects. In order to determine the protective effect on $H_2O_2$-induced oxidative stress, a DCHF-DA (dichlorodihydrofluorescin diacetate) assay was conducted. All fractions, including crude extracts of dried tuna, appeared to significantly reduce the levels of intracellular reactive oxygen species (ROS) with dose responses (p<0.05). Among the fractions, BuOH and 85% methanol fractions showed a higher protective effect on the production of lipid peroxides. These results indicate that the consumption of tuna may be recommended as a potent functional food for preventing cellular oxidation and cancer.
Keywords
Dried tuna; human cancer cell; growth inhibition; antioxidant; reactive oxygen species;
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1 Son, J. Y., J. H. Rhim, and H. S. Son. 1995. Effect of some synthetic and natural antioxidants on the oxidative stability of skip jack oil. Kor. J. Food Nutr. 8, 88-92
2 Takahashi, M., T. Minamoto, N. Yamashita, K. Yazawa, T. Sugimura, and H. Esumi. 1993. Reduction in formation and growth of 1,2-dimethylhydrazine-induced aberrant crypt foci in rat colon by docosahexaenoic acid. Cancer Res. 53, 2786-2789   DOI   ScienceOn
3 Tsuchiya, M., M. Suematsu, and H. Suzuki. 1994. In Vivo visualization of oxygen radical-dependent photoemission. Methods Enzymol. 233, 128-140   DOI
4 Yamada, N., J. Shimizu, M. Wada, T. Takita and S. Innami. 1998. Changes in platelet aggregation and lipid metabolism in rats given dietary lipids containing different n-3 polyunsaturated fatty acids. J. Nutr. Sci. Vitaminol (Tokyo). 44, 279-289
5 Zhang, J., S. Sasaki, K. Amano, and H. Kesteloot. 1999. Fish consumption and mortality from all causes, ischemic heart disease, and stroke: an ecological study. Prev. Med. 28, 520-529   DOI   ScienceOn
6 Nordoy, A., L. F. Hatcher, D. L. Ullmann, and W. E. Connor. 1993. Individual effects of dietary saturated fatty acids and fish oil on plasma lipids and lipoproteins in normal men. Am. J. Clin. Nutr. 57, 634-639
7 Reddy, B. S. and S. Sugie. 1988. Effect of different levels of omega-3 and omega-6 fatty acids on azoxymethaneinduced colon carcinogenesis in F344 rats. Cancer Res. 48, 6642-6647
8 Ruxton, C. H., S. C. Reed, M. J. A. Simpson, and K. J. Millington. 2004. The health benefits of omega-3 polyunsaturated fatty acids: a review of the evidence. J. Hum. Nutr. Diet. 17, 449-459   DOI   ScienceOn
9 Saito, H. and K. Nakamura. 1990. Antioxidative effect of sesamol on fish oil oxidation. Nippon Suisan Gakkaishi. 56, 1893
10 Saito, H., I. Saito, K. J. Chang, Y. Tamura, and S. Yoshida. 1991. Effect of ingestion of eicosapentaenoic acid ethyl- ester on the scavenger activity for acetylated LDL and the production of platelet-derived growth factor in rat peritoneal macrophages. Adv. Prostaglandin Thromboxane Leukot. Res. 21, 241-244
11 Sasagawa, T., K. Kosai, Y. Ota, M. Mori, and M. Okita. 2002. Influences of a dietary fatty acid composition on the emergence of glutathione S-transterase-P (GST-P) positive foci in the liver of carcinogen treated rats. Prostaglandins Leukot. Essent. Fatty Acids 67, 327-332   DOI   ScienceOn
12 Kamali, R. A., J. Marsh, and C. Fuchs. 1984. Effect of n-3 FA on growth of a rat mammary tumors. J. Natl. Cancer Inst. 73, 457-46
13 Shin, M. O., M. J. Ku, and S. J. Bae. 2007. Cytotoxicity and Quinone Reductase activity stimulating effects of fin of Thunnus Thynnus extracts in various cancer cells. Korean J. Nutr. 40, 147-153
14 Simopoppulos, A. P. 1991. Omega-3 fatty acids in health and disease and in growth and development. Am. J. Clin. Nutr. 54, 438-463
15 Jang, J. R., H. J. Choi, K. K. Kim, and S. Y. Lim. 2008. Effect of extracts from dried mackerel on antioxidant activity and inhibition of growth of human cancer cell lines. J. Life Sci. 18, 680-685
16 Kim, S., S. Kang, and H. Choi. 2005. Effects of Dietary levels of corn and tuna oils on the formation of preneoplastic lesions in rat hepatocellular carcinogenesis. Kor. J. Nutr. 38, 20-29
17 Kromhout, D. M., E. B. Bosschieter, and C. Coulander. 1985. The inverse relation between fish consumption and 20-year mortality from coronary heart disease. New Engl. J. Med. 312, 1205-1209   DOI   ScienceOn
18 LeBel, C. P., H. Ischiropoulos, and S. C. Bondy. 1992. Evaluation of the probe 2', 7'-Dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress. Chem. Res. Toxicol. 5, 227-231   DOI   ScienceOn
19 Lee, H. S., H. J. Kim, J. I. Choi, J. H. Kim, J. G. Kim, B. S. Chun, D. H. Ahn, Y. J. Chung, Y. J. Kim, M. W. Byun, and J. W. Lee. 2008. Antioxidant activity of the ethanol extract from cooking drips of Thunnus thynnus by gamma irradiation. J. Kor. Soc. Food Sci. Nutr. 37, 810-814   DOI   ScienceOn
20 Medina, I., S. P. Auboug, and R. P. Martin. 1995. Composition of phospholipids of white muscle of six tuna species. Lipids 30, 1127-1135   DOI   ScienceOn
21 Murase, T. and H. Saito. 1996. The docosahexaenoic acid content in the lipid of albacore Thunnus alalunga caught in the two separate localities. Fish Sci. 62, 634-638
22 Albert, C. M., H. Campos, M. J. Stampfer, P. M. Ridker, J. E. Manson, W. C. Willett, and J. Ma. 2002. Blood levels of long-chain n-3 fatty acids and the risk of sudden death. N. Engl. J. Med. 346, 1113-1118   DOI   ScienceOn
23 Carroll, K. K. 1990. Experimental and epidemiological evidence on marine lipids and carcinogenesis. pp. 99, In Omega-3 Fatty Acids in Health and Disease. Lees, R. S. and Barrel, M. (eds.), ., New York, USA.
24 Chaudry, A., S. McClinton, L. E. F. Moffat, and K. W. J. Wahle. 1991. Essential fatty acid distribution in plasma and tissue phospholipids of patients withbenign and malignantprostate disease. Br. J. Cancer. 64, 1157-1160   DOI   ScienceOn
25 Denizot, F. and R. Lang. 1986. Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliablity. J. Immunol. Methods. 89, 271-277   DOI   ScienceOn
26 Froyland, L., H. Vaagenes, D. K. Asiedu, A. Garras, O. Lie, and R. K. Berge. 1996. Chronic administration of eicosapentaenoic acid and docosahexaenoic acid as ethyl esters reduced plasma cholesterol and changed the fatty acid composition in rat blood and organs. Lipids. 31, 169-178   DOI   ScienceOn
27 Hwang, W. I., Y. H. Ji, M. J. Woo, and J. Y. Lee. 1994. Effects of tuna DHA on the growth of cancer cells, In Vitro. Korean J. Nutr. 7, 655-662
28 Hwang, W. I., N. G. Baik, Y. K. Hwang, and S. D. Lee. 1992. Antitumor and immunological effects of tuna extract. J. Korean Soc. Food Nutr. 21, 353-366