• 한국도로학회:학술대회논문집
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• 한국도로학회 2004년도 학술발표회 논문집
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• pp.111-114
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• 2004

• Journal of Astronomy and Space Sciences
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• 제27권4호
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• pp.263-278
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• 2010

Many filtered CCD measures form the basis of six new light curves of the eclipsing system SW Lyn. From these measures and additional observations for eclipse timing, 47 new times of minimum light over the time-interval of about 13 years have been calculated. The complex period variability can be sorted into a linear period improvement with 5.8-year and 33.9-year periodic terms. The shorter cyclic term of these is ascribed to a cool companion of the eclipsing pair but the longer one has no testable interpretation at present. The new light curves are synthesized by the 2003 version of the Wilson-Devinney differential corrections computer code. The results incorporate a source of "third light" which comes from the cool companion star that had been identified by the cycling of the period of the eclipsing pair and also had previously been identified spectroscopically. There is a measure of satisfaction with current understanding of the SW Lyn eclipsing system because of consistent syntheses of all historical light curves. This agreeable convergence, however, comes partly at the expense of an unanticipated temperature of the hot star and of a photospheric spot that has no obvious basis in the detached character modeled for the binary. We offer predictions of changes in the stellar parameters if the modeled detached-configuration should be wrong. The SW Lyn stellar system is still difficult to understand.

• 한국응용과학기술학회지
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• 제37권6호
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• pp.1764-1772
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• 2020

본 연구는 그라비올라(Annona muricata)가 인간 비만세포(LUVA 세포)에서 immunoglobulin(Ig) E-매개 알러지 반응에 어떠한 효과를 나타내는지 확인하는데 목적이 있다. 그라비올라 잎 추출물(GLE)의 항알러지 효과를 알아보기 위해 IgE로 감작된 비만세포에 다양한 농도의 추출물을 처리하였다. 비만세포의 IgE-매개 탈과립 실험 결과, GLE가 LUVA 세포에서 histamine, β-hexosaminidase, TNF-α, IL-4 및 IL-6의 방출을 유의하게 억제하였다. 또한 GLE는 IgE 유도에 의한 Lyn과 Syk의 인산화를 억제하였고 그 하위 경로에 있는 MAPK의 활성을 억제하였다. 이상의 결과는 GLE가 비만세포 활성화 및 알러지 반응을 억제할 수 있음을 보여주고, 이는 GLE가 항알러지 효과를 통하여 기능성 화장품 소재로서의 활용이 가능함을 시사한다.

• 대한임상검사과학회지
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• 제49권2호
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• pp.128-134
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• 2017

Der p 1과 Der p 2는 알레르기 질환과 관련된 집먼지 진드기의 핵심적인 알러젠이다. 본 연구에서는 Der p 1과 Der p 2가 단구에서 S100A8과 S10A9을 분비시키는지를 확인하였고, 분비된 S100A8과 S10A9이 호중구의 세포고사 조절기전에 작용하는지를 연구하였다. Der p 1과 Der p 2는 정상인의 단구에서 S100A8과 S10A9을 유의하게 증가시켰고, S100A8과 S10A9은 정상인과 알레르기 질환 호중구의 자발적 세포고사를 억제 시켰다. 호중구의 Lyn, Akt, ERK는 S100A8과 S10A9을 시간별로 처리하였을 때 활성화하였다. 본 연구를 통하여 단구와 호중구에서 Der p 1과 Der p 2의 역할을 규명하였고, 나아가 관련된 알레르기 병인기전을 이해하는데 유용할 것이다.

• Biomolecules & Therapeutics
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• 제28권5호
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• pp.456-464
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• 2020

Mast cells (MCs) are systemically distributed and secrete several allergic mediators such as histamine and leukotrienes to cause type I hypersensitivity. Dasatinib is a type of anti-cancer agent and it has also been reported to inhibit human basophils. However, dasatinib has not been reported for its inhibitory effects on MCs or type I hypersensitivity in mice. In this study, we examined the inhibitory effect of dasatinib on MCs and MC-mediated allergic response in vitro and in vivo. In vitro, dasatinib inhibited the degranulation of MCs by antigen stimulation in a dose-dependent manner (IC₅₀, ~34 nM for RBL-2H3 cells; ~52 nM for BMMCs) without any cytotoxicity. It also suppressed the secretion of inflammatory cytokines IL-4 and TNF-α by antigen stimulation. Furthermore, dasatinib inhibited MC-mediated passive cutaneous anaphylaxis (PCA) in mice (ED₅₀, ~29 mg/kg). Notably, dasatinib significantly suppressed the degranulation of MCs in the ear tissue. As the mechanism of its effect, dasatinib inhibited the activation of Syk and Syk-mediated downstream signaling proteins, LAT, PLCγ1, and three typical MAP kinases (Erk1/2, JNK, and p38), which are essential for the activation of MCs. Interestingly, in vitro tyrosine kinase assay, dasatinib directly inhibited the activities of Lyn and Fyn, the upstream tyrosine kinases of Syk in MCs. Taken together, dasatinib suppresses MCs and PCA in vitro and in vivo through the inhibition of Lyn and Fyn Src-family kinases. Therefore, we suggest the possibility of repositioning the anti-cancer drug dasatinib as a treatment for various MC-mediated type I hypersensitive diseases.

• Journal of Ginseng Research
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• 제46권4호
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• pp.550-560
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• 2022

Background: The effect of ginsenoside Rh2 (G-Rh2) on mast cell-mediated anaphylaxis remains unclear. Herein, we investigated the effects of G-Rh2 on OVA-induced asthmatic mice and on mast cell-mediated anaphylaxis. Methods: Asthma model was established for evaluating airway changes and ear allergy. RPMCs and RBL-2H3 were used for in vitro experiments. Calcium uptake, histamine release and degranulation were detected. ELISA and Western blot measured cytokine and protein levels, respectively. Results: G-Rh2 inhibited OVA-induced airway remodeling, the production of TNF-α, IL-4, IL-8, IL-1β and the degranulation of mast cells of asthmatic mice. G-Rh2 inhibited the activation of Syk and Lyn in lung tissue of OVA-induced asthmatic mice. G-Rh2 inhibited serum IgE production in OVA induced asthmatic mice. Furthermore, G-Rh2 reduced the ear allergy in IgE-sensitized mice. G-Rh2 decreased the ear thickness. In vitro experiments G-Rh2 significantly reduced calcium uptake and inhibited histamine release and degranulation in RPMCs. In addition, G-Rh2 reduced the production of IL-1β, TNF-α, IL-8, and IL-4 in IgE-sensitized RBL-2H3 cells. Interestingly, G-Rh2 was involved in the FcεRI pathway activation of mast cells and the transduction of the Lyn/Syk signaling pathway. G-Rh2 inhibited PI3K activity in a dose-dependent manner. By blocking the antigen-induced phosphorylation of Lyn, Syk, LAT, PLCγ2, PI3K ERK1/2 and Raf-1 expression, G-Rh2 inhibited the NF-κB, AKT-Nrf2, and p38MAPK-Nrf2 pathways. However, G-Rh2 up-regulated Keap-1 expression. Meanwhile, G-Rh2 reduced the levels of p-AKT, p38MAPK and Nrf2 in RBL-2H3 sensitized IgE cells and inhibited NF-κB signaling pathway activation by activating the AKT-Nrf2 and p38MAPK-Nrf2 pathways. Conclusion: G-Rh2 inhibits mast cell-induced allergic inflammation, which might be mediated by the AKT-Nrf2/NF-kB and p38MAPK-Nrf2/NF-κB signaling pathways.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2007년도 춘계종합학술대회
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• pp.237-240
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• 2007

이 논문에서는 수정된 Sequential Algorithmic Schema를 이용해서 여러 장소를 이동하면서 찍은 디지털 이미지를 효율적으로 분류할 수 있는 방법을 제안한다. 제안하는 방법은 이웃 패턴들과 특징 정보의 연속성, 유사성을 가지며 들어오는 입력 패턴에 대해 기존의 모든 군집과 유사도를 비교하는 방법이 아니라 이전 군집의 정보와 유사도를 비교하여 군집에 포함시키거나 동적으로 군집을 생성하는 효율적인 군집화 방법이다. 제안한 방법은 실험을 통해서 기존의 군집화 기법에 성능 및 속도의 효율성을 증명하였다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.1685-1686
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• 2008

This paper presents the design and experiment results of a multi-layered microsystem for magnetic bead applications. The magneto-microfluidic device is designed for capable of separating magnetic beads. In the presence of the magnetic field, magnetic beads are attracted and moved to high gradient magnetic fields. A multi-layered microfluidic channel consists of top and bottom layers in order to separate magnetic beads in the vertical direction. Our channel is easily integrated magnetic cell sorter, especially on-chip microelectromagnet or permanent magnet device. Fast separation of magnetic beads in top and bottom channels can be used in high throughput screening to monitor the efficiency of blood and drug compounds.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2007년도 추계종합학술대회
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• pp.977-980
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• 2007

본 논문은 인식된 컨테이너 식별자 문자열과 컨테이너 작업리스트를 비교하여 작업리스트와 인식된 컨테이너 식별자 문자열을 매칭하는 효율적인 방법을 소개하고자 한다. Dynamic Time Warping 기법을 이용하여 오인식되거나 인식이 되지 않은 문자에 대하여 오독률을 최소화할 수 있는 효율적인 방법을 제안한다. 기존의 문자열 비교방식에 비하여 제안하는 방법을 사용하였을 경우 더 나은 성능을 보였다.

• Journal of Advanced Marine Engineering and Technology
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• 제30권5호
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• pp.580-588
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• 2006

Two-dimensional turbulent flows past a square cylinder and cavity noise are simulated by the finite difference lattice Boltzmann method with subgrid turbulence model. The method, based on the standard Smagorinsky subgrid model and a single-time relaxation lattice Boltzmann method, incorporates the advantages of FDLBM for handling arbitrary boundaries. The results are compared with those by the experiments carried out by Noda & Nakayama and Lyn et al. Numerical results agree with the experimental ones. Besides, 2D computation of the cavity noise generated by flow over a cavity at a Mach number of 0.1 and a Reynolds number based on cavity depth of 5000 is calculated. The computation result is well presented a understanding of the physical phenomenon of tonal noise occurred primarily by well-jet shear layer and vortex shedding and an aeroacoustic feedback loop.