• Journal of Life Science
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• v.9 no.6
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• pp.704-708
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• 1999

This research was carried out for evaluating diagnostic value of antibody test in Fragile X syndrome. In antibody test of control individuals and carriers with a premutation, FMRP were detected in the lymphocytes, whereas the lymphocytes of male Fragile X syndrome patients were devoid of FMRP. Five Fragile X syndrome male patient, two Fragile X syndrome female patients, three carriers were diagnosed by southern blot. Five boys who were diagnosed as the patients by antibody test were turned out full mutation and having multiple smear beside normal single band. However, fragile site of X chromosome was not expressed in Fragile X syndrome patients by chromosome analysis. These results showed that antibody test was a fast and simple method, but the diagnostic power was "perpect" for males, whereas the results were less specific for females.r females.

• Korean Journal of Veterinary Research
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• v.31 no.4
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• pp.433-440
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• 1991

Guinea pigs were administrated with T-2 toxin at a rate of 1 and 0.6mg/kg body weight per day for 21 days to study the immunological and pathological effects of T-2 toxin in guinea pigs. Blood was collected before T-2 treatment and on days 7, 14 and 21 of the trial for hematological and biological examinations and for the mitogen assay using lymphocytes. Myeloid: erythroid ratios were examined from the fernur bone marrow samples taken a day before T-2 toxin treatment began, on day 12 and at death. Guinea pigs received with 1mg/kg body weight of T-2 toxin daily showed leukopenic, lymphopenic and anemic signs on day 7 and 14. The mitogenic responses to the T-cell mitogen, Concanavalin A and B-cell mitogens, lipopolysaccharide were significantly depressed on day 7. Histologically, marked cellular damages including karyorrhexis and depletion of lymphocytes were observed in the actively dividing cells of the gastrointestinal tract, lymph node, spleen and bone marrow of guinea pigs.

• Journal of Chest Surgery
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• v.12 no.2
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• pp.101-104
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• 1979

Lymphoid hamartomas are a rare benign disease which can be easily treated by complete surgical excision. They developed most often in the thorax, and can be discovered usually on routine chest X-ray.p But some of them can also be found because of pressure symptoms or the presence of a palpable mass if outside the thorax. We experienced a case of the hyaline-vascular type of lymphoid hamartoma in the left hilum of a 29 year-old Korean male in March, 1979. He was well except intermittent cough and left chest discomfort of a year duration and was treated by resection of the left upper lobe including nodular tumor masses. The histological characteristics were an aggregation of lymphoid follicles composed of concentrically arranged mature lymphocytes with centrally placed thick walled arterioles showing endothelial proliferation and some of them were hyalinized. Between the follicles, there was extensive capillary proliferation and infiltration of numerous lymphocytes, scanty plasma cells and eosinophils.

• The Journal of the Korean Society for Microbiology
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• v.16 no.1
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• pp.65-70
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• 1981

A microculture technique for the mixed lymphocyte culture test(MLC) in the sheep is described. The optimal incubation periods for the MLC are varied from 4 to 6 days depending on the sources of lymphocytes. It is found that a 2.5:1 stimulator-responder cell ratio and the responder cell concentration of $1{\times}10^5$ cells/well produce the highest($^3H$)-thymidine incorporation. Moreover cryopreservation of bovine lymphocytes results in satisfactory and reproducible MLC reaction. It is also evident that the MLC reactions of the sheep are under the control of histocompatibility matching between the stimulator cells and the responder cells. Significance of the technique as a too! for study on cell mediated immunity in sheep system, either normal or squamous cell carcinoma-bearing, are discussed.

• The Journal of Korean Medicine
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• v.17 no.2 s.32
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• pp.303-310
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• 1996

The purpose of this study was to investigate effect of water extract of Carthami Flos on the proliferation of human cancer cell-lines. The effects of Carthami Flos on the proliferation of A431, HeLa, MOLT-4, K562 cells, Balb／c 3T3 cells, mouse thymocytes, splenocytes and human lymphocytes were estimated by MTT colorimetric assay. The results were as follows; 1. Carthami Flos did not effect A431, HeLa, MOLT-4, K562 cells. 2. The cytotoxicity of mitomycin C on K562 cells was increased by the combination of Carthami Flos. 3. Carthami Flos inhibited the proliferation of Balb／c 3T3 cells. 4. Carthami Flos stimulated the proliferation of thymocytes. 5. Carthami Flos stimulated the proliferation of splenocytes. 6. Carthami Flos stimulated the proliferation of human lymphocytes.

• Korean Journal of Animal Reproduction
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• v.17 no.1
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• pp.21-25
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• 1993

We have previously shown that gossypol injection into the stroma of testis was found to effectively inhibit the process of spermatogenesis. In this study, properties and chemistry of blood were investigated weekly in ICR mouse after the injection of gossypol into the testicular stroma(5, 10 or 15mg per kg body weight). There were no significant differences in red blood cell(RBC), hematocrit, white blood cell(WBC), basophils, eosinophils and monocytes during first 4 weeks after injection of gossypol between treatments, but neutrophils increased and lymphocytes decreased, respectively(P<0.05). Total content of protein, albumin and globulin were not different, compared with control. However, the concentration of glucose after injection of gossypol increased significantly(P<0.05). In conculsion, the results of this study indicated that injection of gossypol into stroma of testis might affect both properties and chemistry of blood in mice.

• Journal of Society of Preventive Korean Medicine
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• v.10 no.1
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• pp.13-32
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• 2006

In order to indentify the effect of Astragali Radix(A.R) on cyclophosphamide(C.Y) induced leukopenia, A.R. extracts(EAR) were treated to mice orally, and blood sampling was done by periods. For the in vivo experiments, mice were divided into 4 groups, which treated EAR before, or after C.Y injection, or both, or none. Rapid normalization in the peripheral blood count of WBC, neutrophils, lymphocytes, RBC, and platelets observed in every EAR treated group regardless of the treatment periods of EAR. These studies suggest that, A.R. premedication can be effective in protection of bone marrow suppression during anticancer therapy.

• Journal of Preventive Medicine and Public Health
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• v.17 no.1
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• pp.245-250
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• 1984

Reciprocal exchanges of DNA in sister chromatids (SCEs) are induced by various carcinogens and mutagens, although the quantitative relationship between the number of mutations and SCEs induced varies among chemicals. Nevertheless, the analysis of SCEs production by various agents often proposed as a sensitive and quantitative assay for mutagenicity and cytotoxicity. Mercury, even if which has no evidences for mutagenicity and carcinogenicity, is reported to exert some cytotoxic effects, such as chromosomal aberrations or bad influences to ovulation and reproduction in experimental animals, etc.. In this study, tests for sister chromatid exchanges have been carried out on normal human lymphocytes in whole blood culture to add mercury chloride ($HgCl_2$) or methylmercury chloride ($CH_3\;HgCl$) for 72 hr. The results indicate the dose-dependent relationship between the frequencies of SCEs and the concentrations of $HgCl_2,\;CH_{3}HgCl$ and 5-bromo-2'-deoxyuridine (BrdU). Lymphocyte proliferation has depressed in the higher concentration of mercury.

• The Journal of Korean Medicine
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• v.18 no.1
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• pp.446-448
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• 1997

This experiment was carried out to evaluate the immunological effects of Hwanhonsan extract. Hwanhonsan administration into mice enhanced Arthus reaction and DTH to sheep erythrocytes, and NK cells activities. Hwanhonsan extract augmented the DNA synthesis of mitogen-activated lymphocytes. Hwanhonsan also stimulated leucocyte migration ability, MIF and IL-2 production of T lymphocytes, but not IL-6 production of B cells. These results suggested that effect of Hwanhonsan might be chiefly due to nonspecific enhancement of NK cell activities and cell mediated immune responses.

• Journal of Veterinary Clinics
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• v.20 no.3
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• pp.364-368
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• 2003

Cytogenetic and hematological analysis was performed in peripheral blood from the cattle associated with abnormal delivery around nuclear power plant area. The frequency of micronuclei (MN) in peripheral blood lymphocytes from cattle was used as a biomarker of radiobiological effects resulting from exposure to environmental radiation. An estimated dose of radiation was calculated by best fitting linear-quadratic model based on the radiation-induced MN data over the range from 0 Gy to 4 Gy from the bovine lymphocytes with in vitro irradiation. MN rates in live malformed calf, dams of malformed calves and other cattle living in the same barn from the regions around nuclear power plant, and cattle in control area were 9/1000, 10.8/1000, 13.3/1000 and 10.0/1000, respectively. There were no significant differences in MN frequencies and hematological values between the cattle associated with abnormal delivery around nuclear power plant area and those of control area. This study indicates that the congenital abnormalities near nuclear power plant seemed to be caused by other aetiology.