The Radiobiological Evaluation on Abnormal Delivery of Cattle around Nuclear Power Plant using Micronucleus Assay in Lymphocyte

림프구 미소핵 측정법을 이용한 원자력발전소 주변 소의 이상산에 대한 방사선 생물학적 평가

  • Published : 2003.09.01

Abstract

Cytogenetic and hematological analysis was performed in peripheral blood from the cattle associated with abnormal delivery around nuclear power plant area. The frequency of micronuclei (MN) in peripheral blood lymphocytes from cattle was used as a biomarker of radiobiological effects resulting from exposure to environmental radiation. An estimated dose of radiation was calculated by best fitting linear-quadratic model based on the radiation-induced MN data over the range from 0 Gy to 4 Gy from the bovine lymphocytes with in vitro irradiation. MN rates in live malformed calf, dams of malformed calves and other cattle living in the same barn from the regions around nuclear power plant, and cattle in control area were 9/1000, 10.8/1000, 13.3/1000 and 10.0/1000, respectively. There were no significant differences in MN frequencies and hematological values between the cattle associated with abnormal delivery around nuclear power plant area and those of control area. This study indicates that the congenital abnormalities near nuclear power plant seemed to be caused by other aetiology.

Keywords

References

  1. A review. Appl Radiat Isot v.38 The present state and perspectives of micronucleus assay in radiation protection Almassy,Z.;Krepinsky,A.B.;Bianco,A.;Koteles,G.J. https://doi.org/10.1016/0883-2889(87)90033-5
  2. Sci Total Environ v.274 Pet dogs as sentinels for environmental contamination Backer,L.C.;Grindem,C.B.;Corbett,W.T.;Cullins,L.;Hunter,J.L. https://doi.org/10.1016/S0048-9697(01)00740-9
  3. Curr Proble Pediatr v.14 The effects of ionizing radiation, microwaves, and ultrasound on the developing embryo: Clinical interpretations and applications of the data Brent,R.D.
  4. Radiat Res. v.49 Radiation-induced human chromosome aberration yields following an accidental whole-body exposure to 60Co gamma-rays Brewen,J.G.;Preston,R.J.;Littlefield,L.G. https://doi.org/10.2307/3573421
  5. Mutat Res. v.373 Dose-response of X-irradiated human and equine lymphocytes Catena,C.;Asprea,L.;Carta,S.;Tortora,G.;Conti,D.;Parasacchi,P.;Righi,E. https://doi.org/10.1016/S0027-5107(96)00178-9
  6. Int J Radiat Biol v.76 Analysis of chromosome aberrations in nuclear-power-plant workers considering the lifetime of lymphocytes Chung,H.W.;Kim,S.Y.;Sohn,E.H.;Ha,S.W. https://doi.org/10.1080/09553000050050936
  7. Hereditas v.127 Spontaneous rate of sister chromatid exchanges (SCEs) in mitotic chromosomes of sheep(Ovis aries L.) and comparison with cattle(Bos taurus L.), goat(Capra hircus L.) and river buffalo(Bubalus bubalis L.) Di Berardino D.;Ramunno,L.;Jovino,V.;Pacelli,C.;Lioi,M.B.;Scarfi,M.R.;Burguete,I. https://doi.org/10.1111/j.1601-5223.1997.00231.x
  8. Bone Marrow Trans v.3 Blood cell changes after radiation exposure as an indicator for hemopoietic stem cell function Fieldner,T.M.;Nothdurft,W.;Steinbach,K.H.
  9. Mutat Res v.372 Both bovine and rabbit lymphocytes conditioned with hydrogen peroxide show an adaptive response to radiation damage Flores,M.J.;Pinero,J.;Ortiz,T.;Pastor,N.;Mateos,J.C.;Cortes,F. https://doi.org/10.1016/S0027-5107(96)00082-6
  10. Biomed Environ Sci v.13 Monitoring of human exposure to radiation with the binucleated lymphocyte micronucleus assay He,J.L.;Jin,H.Y.;Jin,L.F.;Gao,S.Y.
  11. Effects of ionizing radiations on the haematopoietic tissue IAEA
  12. A review of methods. IAEA-SM-199/4 Biological dosimetry with particular reference to chromosome aberration analysis IAEA
  13. Technical report 260, IAEA Biological dosimetry : chromosomal aberration analysis for dose assessment IAEA
  14. Anticancer Res v.13 Frequency of micronuclei in lymphocytes following gamma and fast-neutron irradiations Kim,S.H.;Cho,C.K.;Kim,T.H.;Yoo,S.Y.;Koh,K.H.;Yun,H.G.
  15. Mutat Res v.393 Induction of micronuclei in human, goat, rabbit peripheral blood lymphocytes and mouse splenic lymphocytes irradiated in vitro with gamma radiation Kim,S.H.;Han,D.U.;Lim,J.T.;Jo,S.K.;Kim.T.H. https://doi.org/10.1016/S1383-5718(97)00103-4
  16. Radiation-induced chromosome damage in man Cytogenetic effects of ionizing radiations in somatic cells from experimental mammals and extrapolation to man Leonard,A.
  17. Health Phys v.70 Comparison of dose histories for U.S. nuclear power plant workers, based on records held by a major dosimetry service company and on the NRC REIRS database Muirhead,C.R.;Boice,J.D.Jr.;Raddatz,C.T.;Yoder,R.C. https://doi.org/10.1097/00004032-199605000-00004
  18. Int J. Radiat Biol v.59 Biological indicators for radiation damage Muller,W.U.;Streffer,C. https://doi.org/10.1080/09553009114550771
  19. The embryology of domestic animals Cause of congenital malformations Noden,D.M.;DeLahunta,A.
  20. Radiat Protect Dosimetry v.25 Dose assesments by cytogenetic analysis in the Goiania(Brasil) radiation accident Ramalho,A.J.;Nascimento,A.C.H.;Natorajan,A.T. https://doi.org/10.1093/oxfordjournals.rpd.a080358
  21. Mutat Res v.207 Use of frequencies of micronuclei as quantitative indicators of X-ray-induced chromosome aberrations in human peripheral blood lymphocytes: comparison of two methods Ramalho,A.;Sunjevaric,I.;Natarajan,A.T. https://doi.org/10.1016/0165-7992(88)90078-4
  22. Mutat Res v.289 Measurement of micronuclei by cytokinesis-block method in bovine lymphocytes Scarfi,M.R.;Lioi,M.B.;Di Berardino D.;Zeni,O.;Coviello,A.M.;Matassino,D. https://doi.org/10.1016/0027-5107(93)90080-Y
  23. Radiats Biol Radioecol v.43 Dynamics of 134 + 137Cs accumulation in insects inhabiting the 30-kilometer zone of Chernobyl Nuclear Power Station Shliakhtenok,A.S.
  24. Radiats Biol Radioecol v.42 Reconstruction of Ⅰ-131 in milk and exposure doses to the thyroid gland of cattle after the Chernobyl AES Spirin,E.V.
  25. Early Pregnancy v.1 Does occupational nuclear power plant radiation affect conception and pregnancy? Straube,E.;Straube,W.;Romer,T.
  26. Bull environ Contam Toxicol v.66 Micronucleus frequency in cytokinesis-blocked bovine lymphocytes from regions with different pollution levels in Slovakia Sutiakova,I.;Sulik,E.;Rimkova,S.;Sakalikova,A.;Sutiak,V. https://doi.org/10.1007/s00128-001-0027-9
  27. Tierarztl Prax Ausg G Grosstiere Nutztiere v.25 Chernobyl and its consequences-some veterinary medical points of view Tempel,K.
  28. Mutat Res v.445 A cytogenetic study of nuclear power plant workers using the micronucleus-centromere assay Thierens,H.;Vral,A.;Barbe,M.;Aousalah,B.;De Ridder L. https://doi.org/10.1016/S1383-5718(99)00134-5
  29. Int. J. Radiat Biol v.78 Chromosomal radiosensitivity study of temporary nuclear workers and the support of the adaptive response induced by occupational exposure Thierens,H.;Vral,A.;Barbe,M.;Meijlaers,M.;Baeyens,A.;Ridder,L.D. https://doi.org/10.1080/0955300021000034710
  30. Mutagenesis v.15 Cytogenetic monitoring of hospital workers occupationally exposed to ionizing radiation using the micronucleus centromere assay Thierens,H.;Vral,A.;Morthier,R.;Aousalah,B.;De Ridder L. https://doi.org/10.1093/mutage/15.3.245
  31. Mutagenesis v.3 The identification of micronucleated chromosomes: a possible assay for aneuploid Thomson,E.J.;Perry,P.E. https://doi.org/10.1093/mutage/3.5.415