• Horticultural Science & Technology
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• v.32 no.6
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• pp.771-780
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• 2014

In this study, we aimed to understand the effect of sorbitol and salicylic acid (SA) on quality and functional food contents of tomato. Sole or combined application of sorbitol and SA in different concentrations (0.5 mM and 1.0 mM) greatly improved the characteristics of tomato, such as red color tone, soluble solid content, hardness and weight as well as increasing the functional food contents such as of lycopene, anthocyanins and vitamins in comparison with the control. The cyanidin content increased approximately 52-61% and delphinidin contents also increased 20-24% and 39-41% in tomato plants treated with SA alone and sorbitol with SA, respectively. Furthermore, the sole or combined application of sorbitol and SA also increased the vitamin $B_1$ and C contents. In particular, vitamin C content was increased 1.2-fold by treatment with sorbitol alone and was increased 2-fold by SA treatment compared to the control. In the sorbitol with SA treatments, vitamin C content was approximately 4-fold increased relative to control plants.

• Horticultural Science & Technology
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• v.31 no.6
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• pp.833-836
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• 2013

'Hei' is a new cultivar of red with black flesh; originated from the cross of two inbred lines, mother plant, TKUI separated from 'Kame' in 2007 and father plant, TLB separated from tomato collected in Europe by pedigree breeding method. 'Hei' was tested for specific character and productivity of lines of tomatoes in spring for 2 years, 2008 to 2009. This variety had resistance to powdery mildew. 'Hei' is an indeterminate type. The fruit shape is round and the skin color is blackish red. The average fruit weight of 'Hei' is about 127.9 g and marketable yield is $5,715kg{\cdot}10a^{-1}$. Lycopene contents of 'Hei' are about $18.5mg{\cdot}100g^{-1}$, high compared with other tomato ($3.1mg{\cdot}100g^{-1}$). 'Hei' is suitable for environment-friendly protected cultivation because of resistance to powdery mildew.

• Journal of Life Science
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• v.22 no.8
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• pp.1024-1033
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• 2012

A gene encoding a novel geranylgeranyl pyrophosphate (GGPP) synthase from Kocuria gwangalliensis has been cloned and expressed in Escherichia coli. The deduced amino acid sequence showed 59.6% identity with a putative GGPP synthase (CrtE) from K. rhizophila. An expression plasmid containing the crtE gene was constructed, and E. coli cells containing this plasmid produced a recombinant protein with a theoretical molecular mass of 41 kDa, corresponding to the molecular weight of GGPP synthase. Due to the lack of crtE, crtB, and crtI in E. coli, the biosynthesis of lycopene was only obtained when the plasmid pCcrtE was co-transformed into E. coli expressing the pRScrtBI-carrying carotenoid biosynthesis crtB and crtI genes, which were sub-cloned from Paracoccus haeundaensis. The biochemical studies on the expressed proteins were performed via HPLC. The results obtained from this study will provide a wider base of knowledge regarding the primary structure of CrtE cloned from K. gwangalliensis at the molecular level.

• Horticultural Science & Technology
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• v.28 no.5
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• pp.757-761
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• 2010

This research was conducted to investigate effect of ethephon on fruit enlargement and coloring of 'Fuyu' persimmon ($Diospyros$ $kaki$). At 7 weeks after ethephon treatment, fruit weight and diameter became significantly heavier and longer in 80 and $100mg{\cdot}L^{-1}$ than the other concentrations of ethephon. Fruit peel hardness decreased with increasing ethephon concentration. The decreased levels were apparent with the treatments beyond $40mg{\cdot}L^{-1}$ at the beginning, and then with those beyond $60mg{\cdot}L^{-1}$. Hunter $a^*$ and $Chroma^*$ of fruit peel treated with ethephon over $20mg{\cdot}L^{-1}$ were significantly higher compared to those with control ($0mg{\cdot}L^{-1}$), but there were no significant differences among those with the ethephon treatments over $20mg{\cdot}L^{-1}$. The increased levels of the $Chroma^*$ were apparent with the treatments beyond $60mg{\cdot}L^{-1}$ at the beginning and then with those of 20 and $40mg{\cdot}L^{-1}$. ${\beta}$-Carotene and lycopene contents of fruit peel were higher with the ethephon treatment beyond $40mg{\cdot}L^{-1}$. Although the ethephon application at 20 and $40mg{\cdot}L^{-1}$ had no effects on fruit enlargement, but fruit coloring was advanced by 2 weeks with the application at $40mg{\cdot}L^{-1}$.

• Journal of Life Science
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• v.22 no.11
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• pp.1463-1469
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• 2012

To add functional specialty in a traditional fermentation product, Gochujang, and improve the taste and preference, an optimum fermentation condition of Gochujang supplemented with tomato puree was established in the conditions of GCJ 14, -16, -18, and -20. Varying the salinity concentration and the manufactured fermentation products, Gochujang was analyzed by the chemical nature, change in bacterial characteristics and contents of a functional chemical, lycopene, and sensory taste. As a result, the pH change of Gochujang containing tomato puree with grain syrup was diminished during the fermentation process. Its sugar contents were repeated by the increase and decrease. In addition, the water contents, salinity, and chromaticity of the Gochujang showed no significant change. Regarding the change in bacterial characteristics, total bacterial number and lactic acid bacteria number increased, with the rate of increase depending on the fermentation process. The ratio of lactic acid bacteria number against total bacterial number was confirmed to be significantly high at the conditions of GCJ 18 and -20. No significant change in the contents of lycopene was observed during the fermentation process. Notably, the change in crude proteins, crude fat, crude ash, and carbohydrates in addition to a sensory examination including taste and preference of the manufactured Gochujang suggest that the optimal fermentation product is produced in the condition of GCJ 20. Therefore, functional Gochujang satisfying a modern preference can be produced by using tomato puree with grain syrup.

• Culinary science and hospitality research
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• v.21 no.4
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• pp.131-144
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• 2015

The purpose of this study was to determine the best ratio of tomato sauce with chili pepper powder. The investigation of food quality after preparing tomato sauce with chili pepper powder shall be as follows. Moisture was reduced when red chili pepper powder were added gradually, although protein, fat, ash, and carbohydrates were increased. pH values decreased as red chili pepper increased as organic acid was reduced. In case of colour, L-value and a-value were reduced, although b-value increased. Lycopene and ${\beta}$-carotene increased by adding red chili pepper by virtue of the existence of carotenoid. Polyphenol, flavonoid, and DPPH radical also increased with the addition of red chili pepper as a result of red chili pepper's antioxidant property. In an attribute difference test, smoothness showed a low score when red pepper powder was added, but graininess returned a high score as red pepper powder absorbed moisture. The result of the sensory test for sauce, TC2 showed the best score in overall acceptance, while TC3 showed the best score for sauce with pasta in overall acceptance. Tomato sauce with 3% of red pepper powder was shown to have the most acceptable quality and should have powerful manufacture competitiveness.

• Korean Journal of Community Nutrition
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• v.2 no.5
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• pp.728-734
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• 1997

Plasma carotenoid levels were compared among 64 healthy male subjects (control) and 38 patients of ischemic heart disease(IHD) and 20 ones of cerebral infarction(CI) all of whom were over 50years of age. Another 98 healthy male subjects aged 23 to 58 were selected to compare their plasma carotenoid levels by age groups, Levels of lutein ,zeaxanthin and crpytoxanthin were lower in IHD(34$\pm$2, 13$\pm$1 and 62$\pm$7$\mu g$/dl)and CI(36$\pm$3, 12$\pm$2 and 41$\pm$6$\mu g$/dl)patient groups than in control group (84$\pm$5, 16$\pm$2 and 69$\pm$3$\mu\textrm{g}$/dl) while those of lycopene, $\alpha$-and $\beta$-carotene varied little among the three groups. The sum of the six carotenoid levels were levels were, therefore,highest(205$\pm$14$\mu\textrm{g}$/dl) in the control group followed by IHD(155$\pm$15$\mu g$/dl) and CI(128$\pm$17$\mu g$/dl) patient groups, Among the 98 healthy male subject for the age group study, levels of the three major carotenoids increased with age from the twenties to the fifities ; lutein, from 64$\pm$6 to 89$\pm$8$\mu g$/dl, cryptoxanthin, 57$\pm$8 to 73$\pm$4$\mu\textrm{g}$/dl and $\beta$-carotene were more significantly correlated(r=0.30 to 0.61, p<0.01), whereas levels of lycopene and $\alpha$-caroteme were significantly(r=0.21 - 0.23, p<0.05) correlated. (Korean J Community Nutrition 2(5) : 728~734, 1997)

• The Korean Journal of Food And Nutrition
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• v.36 no.1
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• pp.17-24
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• 2023

To apply UV-C as a non-heating sterilization method to increase the microbiological safety of fresh seedless watermelon products, reductions in E. coli and quality changes by treatment dose (0, 2, 4, 8, 14, 20 kJ/m²) were investigated. The pH, sugar content, and hardness of watermelon inoculated with E. coli were not significantly different according to the UV-C treatment dose, but the polyphenol content was significantly decreased compared to the controls (425.4 GAE ㎍/g F.W.). When treated with 2 and 4 kJ/m², the lycopene content was 31.6 and 30.9 ㎍/g F.W., respectively, which was increased compared to the controls (28.5 ㎍/g F.W.). The arginine and citrulline content was also significantly increased compared to the controls. The number of E. coli was significantly decreased compared to the controls following UV-C treatment. Considering the degree of E. coli reduction, lycopene content, arginine content, citrulline content, and UV-C irradiation time, subsequent experiments were conducted by selecting a UV-C treatment dose of 2 kJ/m². The results of confirming the degree of reduction in the number of E. coli colonies by a single treatment and combined treatment with UV-C 2 kJ/m² and 70% ethanol showed that the combined treatment was most effective as colonies were decreased by 2.3 log CFU/g compared to the controls. Therefore, it is judged that UV-C 2 kJ/m² radiation and combined treatment with 70% ethanol could be applied as a non-heating sterilization method for fresh watermelon slices.

• Journal of Photoscience
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• v.9 no.2
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• pp.370-372
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• 2002

In order to investigate effect of the carotenoids (Car) on aggregation of Bacterochlorophyll (BChl) in chlorosome, we studied the spectral difference in aggregates of BChl e formed in the absence and presence of a few kinds of Car in dimethyl sulfoxide (DMSO) -water solution. The absorption spectra of aggregates made of only BChl e and those made of a mixture of BChl e and Car were almost the same. However, the kinetics and circular dichroism (CD) spectra of aggregate of these were markedly different by kind of Car. Specifically, the rate of aggregation for a mixture of BChl e and isorenietene that contains phenyl as end groupe was faster than that for only BChl e. CD spectra of aggregates made of a mixture of BChl e and isorenietene dramatically changed compared to that made of only BChl e. We propose that BChl might form several kinds of rod-like supramolecular structures to in the presence of some kind of Car in chlorosome.

• Journal of the Korean Applied Science and Technology
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• v.32 no.2
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• pp.302-311
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• 2015

To better investigate the antioxidative property of thermal treatment yam (Dioscorea batatas DECNE.) in Korea, some established methods were used. 70% Methanol, 70% ethanol and chloroform-methanol (CM, 2:1, v/v) extracts were collected. 70% Methanol extract exhibited stronger antioxidative activity evaluated by ferrous ion chelating activity, NO radical scavenging activity and ${\beta}$-carotene bleaching assays. On the contrary, CM extract was the most effective in inhibiting linoleic acid peroxidation. Yam available in Korea was also analyzed for its bioactive compounds such as lycopene, chlorophyll a, b, tannin, phytic acid and total saponin contents. Total saponin was abundant in thermal treatment, which determined to be $42.52{\pm}1.88mg/g$. Based on the results obtained from this study, thermal treatment yam could be used as natural antioxidant source due to its high antioxidant activity and bioactive compound contents.