• 동아시아식생활학회지
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• 제9권3호
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• pp.363-369
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• 1999

The heavy metal removability of four kinds of tea was investigated in the various conditions: particle size of tea(10, 35. 70, 100 mesh), concentration of heavy metal(25, 50, 100ppm) and extraction temperature(30. 50, 70. 10$0^{\circ}C$). The removabilities by the tea solids were increased as the particle size decreased, concentration of heavy metal increased, and extracting temperature increased. Of the four kinds of tea Pb, Cd and Cu removability by Eucommia ulmoides Oliv tea was the highest. In addition, heavy metal removability by Schizandra chinensis Baillon tea was better than that of other tea as extraction temperature was increased.

• Journal of Plant Biotechnology
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• 제6권3호
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• pp.157-163
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• 2004

An efficient system for the regeneration of adventitious shoots from in vitro cultured leaf sections of Lycium chinense Miller was developed and silent somaclones from the regenerants detected with RAPD method. Among the eight media tested (B5, SH, N&N, 1/2MS, MS, 3/2MS, GD and WPM), and four cytokinins (BA, kinetin, 2ip and zeatin) with different concentrations (1, 5, 10, 20, 30 and 40 $\mu{M}$), 1/2 MS medium supplemented with 20 and 30 $\mu{M}$ zeatin showed the best regeneration frequency (100% and 93.7%) and higher average number of shoots (9.0 and 9.4). All regenerants easily elongated after subculturing on 1/4MS without growth stimulants and produced spontaneous adventitious roots from their basal parts. With phenotypically normal 40 regenerants, RAPD analysis with 15 different random primers was performed to examine the cryptic somaclonal variants. No substantial differences in banding patterns were found in the amplified polymorphic DNAs implying no DNA changes during dedifferentiation into adventitious shoots. However, one (OPF-4) of the 15 primers detected silent somaclonal variation in one regenerant in which two different polymorphic bands did not appear when compared with the rest regenerants. The results indicate that regenerantion via intervening callus phase can be used to establish true-to-type planting stocks for homogeneous population.

• Applied Biological Chemistry
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• 제39권5호
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• pp.338-342
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• 1996

한방에서 강장과 보간 등에 효과가 있는 것으로 알려진 구기자를 원료로 술을 양조하여 그 일반성분, 유기산, 메탄올, ester, 휴젤유 및 $SO_2$ 등을 조사하였고, $20^{\circ}C$와$30^{\circ}C$에서 20일 저장하면서 위의 성분분석과 관능 검사를 하였다. 보당형 구기자술 중의 유기산량은 젖산, 능금산, 주석산 및 초산 등이 주를 이루었으며 ester 및 휴젤유는 각각 $4.0{\sim}5.0\;mg%$와 $12{\sim}30\;mg%$ 그리고 methanol은 $25{\sim}27\;mg%$이었다. 약주형 구기자술의 ester 및 휴젤유는 보당형보다 많았다. 한편, 보당형 및 약주형 구기자술의 주질에 미치는 저장온도는 $30^{\circ}C$보다 $20^{\circ}C$가 좋았으며 살균 처리법은 $SO_2$ 100 ppm 첨가 또는 $60^{\circ}C/30$분의 처리가 좋았다.

• 한국약용작물학회지
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• 제7권2호
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• pp.89-93
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• 1999

구기자의 비닐 하우스 재배에서 재배방법에 따른 생육, 수량성 및 병해충 발생정도를 알아보기 위하여 충남 청양군 청양농공업고등학교 실습 포장의 비닐 하우스에서 1997년 2월 20일부터 98년 10월5일까지 ‘청양 재래’ 품종을 재식거리, 적심시기 및 적심횟수를 달리하여 재배한 시험 결과를 요약하면 다음과 같다. 1. 재식거리가 멀수록 분지수가 적어졌고, 경장, 경직경, 분지당 엽수, 엽장, 엽폭은 증가하였으며, 개화기 차이는 없었다. 2. 재식거리가 멀수록 분지당 화수, 착과수, 생과중, 전과중은 증가하였으나, 총 수량은 단위면적당 총 주수가 많은 재식거리 $60\;{\times}\;20cm$에서 가장 높았다. 3. 적심시기가 5월5일에서 6월5일로 늦어질수록 생육형질인 경장, 경직성, 분지수, 분지당 엽수, 엽장, 엽폭은 낮았고, 분지당 화수, 착과수, 생과중, 건과중도 모두 낮았다. 4. 적심횟수가 증가할수록 분지수는 많아졌지만, 경장, 경직경, 분지당 엽수, 엽장, 엽폭 모두 감소하였다. 5. 적심횟수가 증가할수록 분지당 개화수, 과립수, 착과율은 감소하였지만, 주당 생과중, 건과중은 증가하였다.

• 한국약용작물학회지
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• 제4권1호
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• pp.58-63
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• 1996

구기자(枸杞子) 재배시(栽培時) 생육환경(生育環境)을 개선함으로써 건전한 생육(生育)을 도모하여 수양성(收量性)을 증대하고 농약방제(農藥防除)에 소요되는 노력을 절감하면서 안전 농산물(農産物)을 생산키 위하여 1992년(年) 비가림 망실재배시험(網室栽培試驗)을 수행하였던 바 그 결과를 요약하면 다음과 같다. 1. 평균(平均) 기온(氣溫)은 비가림 망실재배시(網室栽培時) 노지자연재배에 비하여 $1{\sim}1.9^{\circ}C$가 높았고, 병충해 방제횟수는 비가림 망실재배가 2회, 노지재배가 7회이었다. 2. 지상부(地上部) 생육(生育)은 망실재배(網室栽培)가 노지재배에 비하여 양호하였다. 3. 비가림망실재배가 노지재배에 비하여 탄저병의 이병과율과 혹응애 발생을 현저히 감소시켰다. 4. 수량은 비가림 망실재배시 환기처리 204kg, 미환기처리 218kg으로 노지재배에 비하여 $92{\sim}106%$ 증수하였고, 이는 병충해 감소에 의한 주당과수의 확보에 기인하였다.

• 한국자원식물학회지
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• 제1권1호
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• pp.42-47
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• 1988

생약식물로서 중약한 위치를 점하고 있는 구기자의 전지정도에 따른 품질 및 수량반응을 구명하기 위하여 1982 ~ '83년 2개년에 걸쳐 "진도재래"를 공시시험한 결과는 다음과 같다. 1. 구기자의 개화반응은 맹모 - 황엽 - 낙엽 - 개화의 일련의 단계를 거쳐 개화되었고, 2. 무전지구에 비해 전지구가 간장이 길고 그에 따른 절수도 많았으며 총 유효분지수가 많아 수량에 영향을 주었다. 3. 무전지구에서는 과정이 짧아 과실이 잘고 전지구는 과정이 길고 굵은 충실한 과실이 생산되어 상품가치가 높았다. 4. 10 a 당 수량은 무전지 방치구(76kg)에 비하여 지표면 전지구와 지표면 10 cm 전지구에서 38 ~ 39%의 증수효과가 있어 구기자는 모년 무전하여 재배하는 것이 바람직하다고 사료된다. 5. 구기자 수확회수별 수량은 2회 수확으로 생산량의 85%를 수확할 수 있어 수학노력 절감이 앞으로의 과제라 하겠다.

• Journal of Microbiology and Biotechnology
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• 제27권8호
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• pp.1386-1391
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• 2017

The bioactivities of boxthron fruits, a source of oriental medicine, are well known, whereas phytochemical studies of the boxthorn stem are rare. In this study, the stem extract of boxthorn (Lycium chinense Miller) and its subfractions were evaluated for their effects on nitric oxide (NO) inhibition and procollagen type I peptide (PIP) synthesis. A phenolic amide isolated from the stem extract was also assayed for these effects. The compound, N-trans-feruloyltyramine, was identified by $^1H$, $^{13}C$, and 2D-nuclear magnetic resonance analyses. In NO inhibition, the chloroform fraction (CF) exhibited the strongest inhibitory activity ($MIC_{50}=24.69{\mu}g/ml$) among the subfractions of the ethanol extract (EE). N-trans-feruloyltyramine isolated from the CF showed strong NO inhibitory activity, presenting with an $MIC_{50}$ of $31.36{\mu}g/ml$. The EE, CF, and N-trans-feruloyltyramine shown to have NO inhibition activity were assayed for the activity of PIP synthesis. The EE and CF showed relatively high PIP values of 38.8% and 24.21% at $100{\mu}g/ml$, respectively. The PIP value for $20{\mu}g/ml$ N-trans-feruloyltyramine showed a 36% increase compared with the non-treated control, whereas that treated with $20{\mu}g/ml$ ascorbic acid as a positive control showed a 13% increase. The results suggest that the proper stem extract of boxthorn stem could be efficiently used to produce good cosmetic effects.

• 한국식품조리과학회지
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• 제22권6호통권96호
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• pp.832-839
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• 2006

Kugija was added to Nabak kimchi to improve the quality and preservation and the optimum addition level was assessed. Kugija extract was prepared by boiling kugija fruits, at different ratios (0, 1, 3, 5, 7%; w/v) in water for 30 minutes. The changes in the physicochemical properties of the Nabak kimchi were measured during storage for 25 days at 10?, and compared to a control (without kugija). The pH was decreased in all treatments. Following the fermentation of Nabak kimchi, the total acidity values were inversely proportional the pH changes according to the nature of mutual dependence. However, in short term, during the initial 7 days of fermentation, the total acidity values decreased with increasing concentrations of kugija extract, whereas the trend was reversed after day 10. Total vitamin C content was directly proportional to the concentration of kugija extract and was decreased with the laps of fermentation. Up to day 25, 7% treatment showed the highest vitamin C content, but at 25 days 1% and 3% treatments ranked the first. The mont of reducing sugar was proportional to the concentration of kugija extract however, the difference of values between all treatments became almost indiscernible after day 25. Turbidity values were generally increased in all samples during fermentation period, although only to a limited extent. The lowest turbidity was shown at 3% treatment up to day 16. Total color difference values were increased up to day 16, but then decreased. The optimum level of kugija extract in Nabak kimchi, as determined through these experiments, was between 1 to 3% per added water content, and was preferably 3% for color and fermentation-retarding effect of the product. Kugija extract could be applied for improving the quality and preservation of traditionally prepared Nabak kimchi.

• 한국약용작물학회지
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• 제3권1호
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• pp.9-11
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• 1995

최근(最近) 재배(栽培) 면적(面積)과 수요(需要)가 급증(急增)되고 있는 구기자(拘杞子) 재배시(栽培時) 치명적(致命的) 피해(被害)를 주는 구기자(拘杞子) 탄저병(炭疽病)에 대한 약제(藥劑) 시험(試驗) 결과(結果) 1. 탄저병(炭疽病) 발병(發病) 시간(時間)는 7월 중순(中旬)과 8월 중순이 가장 심(甚) 하였으며 평균온도(平均溫度) $25^{\circ}C$ 전후(前後)의 조건하(條件下)에서 발병율(發病率)이 가장 높았다. 2. 운곡면(雲谷面)과 대치면(大?面) 모두 프로피 수화제의 방제가(防除價)가 81.5%와 93.4%를 나타내어 약효(藥效)가 가장 우수(優秀)하였다. 3. 디치돈 수화제와 프로피 수화제 모두 기준량(基準量)과 배양(倍量) 공히 약해(藥害)가 발생(發生)하지 않았다.

• 한국식품저장유통학회지
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• 제7권1호
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• pp.68-73
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• 2000

These experiments were performed to investigate the safety of two herbs-Houttuynia cordata Thunberg and Lycium chinese Miller-irradiated with gamma-rays in respect of genotoxicity. Water extracts from the 10 kGy gamma-irradiated herbs were examined in two short -term in vitro tests ; (1) Salmonella typhimurium reversion assay (Ames test) in strain TA 98 and Ta100 and (2) Micronuclues test on clutured Chinese hamster ovary(CHO) cells. No mutagenicity was detected in the two assays with or without metabolic activation . From these results , the safety of the herbs irradiated with gamma-rays at practical doses could be revealed in further tests of genotoxicity in vivo, chronic and reproductive toxicity.