• Proceedings of the KIEE Conference
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• 2002.07c
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• pp.1552-1554
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• 2002

본 논문에서는 실리콘을 기초로 한 포토다이오드를 제작하여 현재 바이오센서에서 이용되고 있는 luminol 화학 발광을 전기적으로 검출하였다. 우선, 실리콘 웨이퍼에 이온주입을 통해 p-n 접합을 형성하고 Al 전극을 형성시켜 포토다이오드를 제작하였다. PDMS을 passivation 막으로 사용하여 horseradish peroxidase(HRP) 농도 변화에 따른 발광의 최적조건과 luminol, HRP가 섞인 용액에 서로 다른 양의 과산화수소를 넣어 발광이 최대로 발생하는 혼합비를 측정하였다. 최종적으로 2mM luminol, 25U HRP 조건 하에서 과산화수소 농도에 따른 화학 발광을 측정하였으며, $10{\mu}M$부터 $500{\mu}M$ 범위의 과산화수소 농도 변화에 따른 포토다이오드의 감도는 단위 면적당 23.429pA/ ${\mu}M$이다.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2002.11a
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• pp.121-122
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• 2002

PAN (Peroxyacetyl Nitrate)은 오존과 함께 대도시 광화학오염의 주요 원인물질로 알려져 있고 대기 중 NOx와 VOCs가 PAN의 생성에 복잡한 반응 경로를 통해 영향을 줄 뿐 만 아니라 원거리 이동을 통하여 비교적 청정지역에 주요한 NOx 공급원으로 알려져 있다. 하지만 PAN은 대기 중 농도가 작고 비교적 낮은 온도에서도 쉽게 열분해 되기 때문에 관측이 어려운 물질이다. (중략)

• Bulletin of the Korean Chemical Society
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• v.26 no.12
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• pp.1937-1940
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• 2005

A lab-made chemiluminescence system with air pump was developed for monitoring of some metal levels in rain. The air pump enabled injection of 17.7 $\mu$g samples into a glass cell filled with luminol-$H_2O_2$ reagent of typically 300 $\mu$L for chemiluminescence measurement. The monitored trend of total metal ions in the rain collected in our campus was compared with analytical results of each metal ion from GFAAS. The system was also demonstrated to determine $Cr^{6+}$ by reduction to $Cr^{3+}$ using $SnCl_2$. The limit of detection for $Cr^{6+}$ obtained by 4 measurements was 85.0 pg $mL^{-1}$ with a relative standard deviation of 3.4%. Although this system doesn’t have selectivity due to the characteristics of chemiluminescence, application of it to environmental monitoring as a sensor for some transition metal ions was demonstrated.

• Fisheries and Aquatic Sciences
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• v.6 no.4
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• pp.209-212
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• 2003

Lucigenin (Lg)- and luminol (Lm)-dependent chemiluminescence (CL) was used to compare the respiratory burst of rockfish (Sebastes schlegeli) phagocytes after stimulation with phorbol myristate acetate (PMA). To establish which reactive oxygen species (ROS) contributes to the observed CL, the modulators of ROS metabolism, such as superoxide dismutase (SOD), catalase, and sodium azide $(NaN_3)$ were used. Although LgCL responses were inhibited significantly by the addition of either SOD or catalase, in comparison to the control, significantly lower LgCL responses were recorded by SOD than catalase. LmCL also showed significantly decreased responses by the addition of SOD and catalase. However, there were no statistical differences in CL responses between SOD and catalase additions. More profound and significant decrease of LmCL responses were recorded by simultaneous addition of SOD and catalase. Sodium azide markedly enhanced LgCL responses, while it significantly inhibited LmCL responses. These results indicate that LgCL and LmCL can be used to measure extracellular $O_2$ production and myeloperoxidase (MPO)-mediated ROS production in fish phagocytes, respectively. Furthermore, LmCL can be used for analyzing intracellular ROS production by simultaneous addition of both SOD and catalase.

• Journal of the Korean Chemical Society
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• v.45 no.3
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• pp.223-229
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• 2001

A method for the determination of glucose in human whole blood by chemiluminescence method using a stopped flow injection system has been studied. The method is based on the differences in the chemiluminescence intensities of luminol due to the different amounts of hydrogen peroxide produced from the glucose oxidase catalyzed reaction. The enzyme reactor was prepared by immobilization of glucose oxidase on aminopropyl glass beads and the chemiluminescence from a flow cell was measured by means of an optical fiber bundle. In order to obtain the optimum experimental conditions, effects of pH for the chemiluminogenic solution and enzyme reactor, flow rate and temperature on the chemiluminescence intensity were investigated. The calibration curve obtained under optimum experimental conditions was linear over the range from $1.0{\times}10^{-1}$ mM to 7.0 mM and the detection limit was $6.0{\times}10^{-2}$ mM. The proposed method was applied to the determination of glucose in whole human blood sample and the results were compared with those obtained by an official method. The present method was also evaluated by the results of recovery experiments.

• Applied Chemistry
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• v.15 no.2
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• pp.113-116
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• 2011

A sensitive and selective determination method of Fe(II) ion by luminol-H₂O₂ system using a chelating reagent has been presented. A metal ion-chelating ligand complex such as Fe(II)-diethylenetriamine pentaacetic acid (DTPA) produced higher chemiluminescence (CL) intensity as well as longer lifetime in luminol-H₂O₂ system than metal exist as free ions. Furthermore, the catalytic activity of Cu(II) and Pb (II) complexes with chelating reagents in luminol-H₂O₂ system was lost since chelating reagents act as a masking agent although free Cu(II) and Pb(II) ions have high catalytic activity. On the optimized conditions, the calibration curve of Fe(II) ion was linear over the range from 1.0×10^-7 to 2.0×10^-5 M with correlation coefficient of 0.996. The detection limit was calculated to be 4.0×10^-8 M.

• Journal of the Korean Society of Clothing and Textiles
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• v.45 no.5
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• pp.840-851
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• 2021

In this study, we used a chemiluminescence reaction to investigate the removal effect of pet soil, such as dog blood, urine and feces. The soiled fabrics were washed with a standard laundry course of 30℃ and a washing time of 30 min and a pet care laundry course of 40-60℃ and a washing time of 100 min. The detergency was evaluated by the surface reflectance and chemiluminescence reaction (bloodstain detection by luminol test and urine-stain and feces-stain detection by UV blacklight test) before and after washing. The surface reflectance results did not show any difference in detergency for both courses, whereas the chemiluminescence reaction did. The detergency of the pet care course compared to the standard course was 101% according to the surface reflectance and 120% according to the chemiluminescence reaction. Therefore, residual stains not detected by surface reflectance can be evaluated through chemiluminescence reaction, and it was confirmed that pet stains can be managed more hygienically by washing for a long time at a high temperature.

• Analytical Science and Technology
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• v.24 no.4
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• pp.243-248
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• 2011

A selective determination method of mercury (II) ion in aqueous solution by luminol-based chemiluminescence system (luminol CL system) has been developed. Determination of metal ions such as copper (II), iron (III), chromium (III) ion in solution by the luminol CL system using its catalytic role in the reaction of luminol and hydrogen peroxide has been reported by several groups. In this study, the catalytic activity of mercury (II) ion in the reaction of luminol and hydrogen peroxide was observed by the enhanced CL intensity of the luminol CL system. Based on this phenomenon, experimental conditions of the luminol CL system were investigated and optimized to determine mercury (II) ion in aqueous solution. While mercury (II) ion in mixed sample solution containing mercury (I) and (II) ions highly enhanced the CL intensity of the luminol CL system, the mercury (I) ion could not enhanced the CL intensity. Thus selective determination of the mercury (II) ions in a mixture containing mercury (I) and (II) ions could be achieved. Each concentration of mercury (I) and (II) ions in aqueous solution can be obtained from the results of the CL method that give the concentration of only mercury (II) ion and the inductively coupled plasma (ICP) method that give the total concentration of mercury ions. On the optimized conditions, the calibration curve of mercury (II) ion was linear over the range from $1.25{\times}10^{-5}$ to $2.50{\times}10^{-3}M$ with correlation coefficient of 0.991. The detection limit of mercury (II) ion in aqueous solution was calculated to be $1.25{\times}10^{-7}M$.

• Asian Journal of Atmospheric Environment
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• v.7 no.2
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• pp.105-113
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• 2013

We constructed and tested an airborne peroxyacetyl nitrate (PAN) monitoring system based on luminol chemiluminescence detection with fast gas chromatography. This system allowed for simultaneous measurement of PAN and nitrogen dioxide ($NO_2$) with a time resolution of <2 min. Actual sample masses within the fixed volume sample loop at various altitudes and temperatures were adjusted to standard atmosphere, using measured pressures and temperatures. The airborne PAN measurement system was evaluated during two field studies above the southern Korean Peninsula in August and October 2009. The detection limit based on the ISO approach was 0.035 ppbv PAN, well below the observed concentrations of 0.185-1.49 ppbv during these studies. Under these conditions, the PAN mixing ratios were positively correlated with $O_x$ ($O_x=O_3+NO_2$), with slopes varying between 0.014 and 0.033 and intercepts between 22.6 and 55.1 ppbv $O_x$. The intercepts corresponded roughly to background $O_x$ mixing ratios in central Europe; however, the slopes were above the range of slopes reported in other studies. We also enhanced the durability, safety, and ease of maintenance of the PAN monitoring system by redesigning the structure of the conventional luminol cell.

• Bulletin of the Korean Chemical Society
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• v.29 no.8
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• pp.1525-1532
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• 2008

An instrument was developed for the simultaneous determination of gas- and aerosol-phase nitrous acid (HONO). Gaseous HONO (HONO(g)) was sampled by a diffusion scrubber and particulate nitrite ($NO_2\;^-$(p)) was collected by a particle growth chamber. The collected samples were analyzed in time-sharing manner, based on the peroxynitrite-induced luminol chemiluminescence. The automated system was found to be sensitive with 13 pptv of detection limit, fast with 4 min. of sampling frequency, and simple and affordable to construct and operate. The system was optimized by adjusting the experimental parameters. The system was applied to the field measurement of gas- and particle-phase HONO during the springtime of 2004 in Gwangju, South Korea. HONO(g) concentrations varied diurnally from 200 pptv around 3 P.M. to 800 pptv at 5 A.M. The variation of $NO_2\;^-$(p) was not significant with the maximum of 240 pptv at 11 P.M. and the minimum of 170 pptv at 4 P.M., not displaying distinct characteristics.