Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Comparison between Lucigenin- and Luminol-dependent Chemiluminescence Responses of Rockfish (Sebastes schlegeli) Head Kidney Phagocytes

  • Jung Jae Hyuck (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kwon Se Ryun (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Lee Eun Hye (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim Sung Mi (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Jeong Hyun Do (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Chung Joon Ki (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim Ki Hong (Department of Aquatic Life Medicine, Pukyong National University)
  • Published : 2003.12.01
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Abstract

Lucigenin (Lg)- and luminol (Lm)-dependent chemiluminescence (CL) was used to compare the respiratory burst of rockfish (Sebastes schlegeli) phagocytes after stimulation with phorbol myristate acetate (PMA). To establish which reactive oxygen species (ROS) contributes to the observed CL, the modulators of ROS metabolism, such as superoxide dismutase (SOD), catalase, and sodium azide $(NaN_3)$ were used. Although LgCL responses were inhibited significantly by the addition of either SOD or catalase, in comparison to the control, significantly lower LgCL responses were recorded by SOD than catalase. LmCL also showed significantly decreased responses by the addition of SOD and catalase. However, there were no statistical differences in CL responses between SOD and catalase additions. More profound and significant decrease of LmCL responses were recorded by simultaneous addition of SOD and catalase. Sodium azide markedly enhanced LgCL responses, while it significantly inhibited LmCL responses. These results indicate that LgCL and LmCL can be used to measure extracellular $O_2$ production and myeloperoxidase (MPO)-mediated ROS production in fish phagocytes, respectively. Furthermore, LmCL can be used for analyzing intracellular ROS production by simultaneous addition of both SOD and catalase.

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References

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