• Title/Summary/Keyword: Lucilia

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Ultrastructure of the Ventral Nephrocytes in the Larva of Lucilia illustris Meigen (연두금파리 종령유충의 복신세포의 미세구조)

  • Cho, Jeong-Sook;Kim, Kwan-Seon;Kim, Woo-Kap
    • Applied Microscopy
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    • v.21 no.2
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    • pp.29-38
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    • 1991
  • Ventral nephrocytes in the larva of the Lucilia illustris comprise ellipsoid cells situated onto the salivary glands. The cells are $60{\sim}100{\mu}m$ in diameter. Junctional complex beneath the basement membrane hold the plasma membrane in a even contour. Intracellular channels from the juntion complex are well developed at the cortex part of the cell. Coated vesicles pinched off from the channels seems to be connected with the ${\alpha}$-vacuoles via the tubular elements, which is regared as selective absorption system from the hemolymph. Two nuclei are sometimes observed in the medulla part of the cell. Ventral nephrocytes contain well-developed rough endoplasmic reticulum and Golgi complex, and numerous mitochondria. These cellular organelles synthesize lysosome. The lysosome not only digest some cell organells but also seems to be related with the ${\beta}$-vacuoles.

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An aural myiasis case in a 54-year-old male farmer in Korea

  • Cho, Jin-Hak;Kim, Hyung-Baek;Cho, Chin-Saeng;Hun, Sun;Ree, Han-Il
    • Parasites, Hosts and Diseases
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    • v.37 no.1
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    • pp.51-53
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    • 1999
  • A 54-year-old male farmer residing in Chunchon, Korea. complaining of blood tinged discharge and tinnitus in the left ear for two days. was examined in August 16, 1996. Otoscopic examination revealed live maggots from the ear canal. The patient did not complain of any symptoms after removal of maggots. Five maggots recovered were identified as the third stage larvae of Lucilia sericata (Diptera: Calliphoridae). This is the first record of aural myiasis in Korea.

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A Nasal Myiasis in a 76-Year-Old Female in Korea

  • Kim, Jae-Soo;Seo, Pil-Won;Kim, Jong-Wan;Go, Jai-Hyang;Jang, Soon-Cheol;Lee, Hye-Jung;Seo, Min
    • Parasites, Hosts and Diseases
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    • v.47 no.4
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    • pp.405-407
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    • 2009
  • On July 2009, 5 fly larvae were discovered inside the nose of a 76-year-old female. She was living in Cheonansi, and in a state of coma due to rupture of an aortic aneurysm. Surgery was performed on the day of admission, and the larvae were found 4 days later. By observing their posterior spiracle, the larvae were identified as Lucilia sericata. Considering the rapid development of this species, the infection was likely acquired during hospitalization. Further investigation on the hospital environment should be needed to know the origin of the infection.

Wariations in cyclic AMP, cyclic AMP-Dependent Protein Kinase and Ecdysteroids during Development of the Blowfly, Lucilia illustris (연두금파리의 발생에 따른 cyclic AMP, cyclic AMP-Dependent Protein Kinase, Ecdysteroids의 변화)

  • 김유경;류진수이경로
    • The Korean Journal of Zoology
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    • v.37 no.4
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    • pp.565-572
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    • 1994
  • 연두금파리의 유충-번데기-성충의 발생에 따른 cyclic AMP(CAMP)와 ecdysteroids의 농도 및 cyclic AMP-dependent protein kinase(PKA)의 활성도를 High Performance Liquid Chromatography(HPLC)와 Liquid Scintillation Counter(LSC)를 이용하여 측정하고 상관 기능 변화를 조사하였다. CAMP의 농도와 PKA의 활성도는 종령유충 및 성충에서 낮은 갓(0.29 UM/g, 5.52~5.59 unit/mg)을 나타내었고 번데기 0일(0.49 UM/g. 92.22 unit/mg)과 7일(0.50UM/g, 24.45 unit/mg)에서 최고치를 보였으며 번데기 4일에서 최저수준(0.13 $\mu$M/g3.23 unit/mg)을 나타내었다. Ecdysone 농도는 번데기 2일에 최고치(37 84 USlg)를 4일에 최저치(18.46 Ugyg)를 보인 후, 5일에 24.37 1519로 상승하였으며 성충에서는 낮은 값을 나타내었다 그러나 20-hydroxvecdysone 농도는 번데기 4일(23.66 UgyS)과 번데기 6일(14.90 Ugyg)에 최고치를 이루었고, 7일에서 최저치(1.21 USi9)를 나타내었다.

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Forensically Important Blow Flies Chrysomya pinguis, C. villeneuvi, and Lucilia porphyrina (Diptera: Calliphoridae) in a Case of Human Remains in Thailand

  • Monum, Tawatchai;Sukontason, Kabkaew L.;Sribanditmongkol, Pongruk;Sukontason, Kom;Samerjai, Chutharat;Limsopatham, Kwankamol;Suwannayod, Suttida;Klong-klaew, Tunwadee;Wannasan, Anchalee
    • Parasites, Hosts and Diseases
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    • v.55 no.1
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    • pp.71-76
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    • 2017
  • This is the first study to report Chrysomya pinguis (Walker) and Lucilia porphyrina (Walker) (Diptera: Calliphoridae) as forensically important blow fly species from human cadavers in Thailand, in addition to Chrysomya villeneuvi (Patton) already known in Thailand. In 2016, a fully decomposed body of an unknown adult male was discovered in a high mountainous forest during winter in Chiang Mai province. The remains were infested heavily with thousands of blow fly larvae feeding simultaneously on them. Morphological identification of adults reared from the larvae, and molecular analysis based on sequencing of 1,247 bp partial mitochondrial cytochrome c oxidase subunit 1 gene (CO1) of the larvae and puparia, confirmed the above mentioned 3 species. The approving forensic fly evidence by molecular approach was described for the first time in Thailand. Moreover, neighbor-joining phylogenetic analysis of the CO1 was performed to compare the relatedness of the species, thereby affirming the accuracy of identification. As species of entomofauna varies among cases in different geographic and climatic circumstances, C. pinguis and L. porphyrina were added to the list of Thai forensic entomology caseworks, including colonizers of human remains in open, high mountainous areas during winter. Further research should focus on these 3 species, for which no developmental data are currently available.

Selection of Rearing Media, Proper Temperature and Period for Storage of Sterile Lucilia sericata (Diptera: Calliphoridae) Eggs and Larvae (무균 구리금파리 유충 생산용 배지의 선발과 알 및 유충의 저장을 위한 온도 및 기간)

  • Jang, Sin-Ae;Yun, Ji-Eun;Park, Chung-Gyoo
    • Korean journal of applied entomology
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    • v.48 no.2
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    • pp.269-274
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    • 2009
  • Rearing media were selected for the production of sterile larvae of greenbottle blowfly, Phaenicia (=Lucilia) sericata (Meigen) which is widely used in maggot therapy. Eight media available in the market were used in this study. Egg hatchability was not different among the media. Survivorship of the larvae was higher in blood agar (BA), sabouraud dextrose agar, and brucella blood agar than the others. A higher content (20${\sim}$40%) of sheep blood in BA and chocolate agar increased the survivorship of larvae. The eggs and the early 3rd larvae could be stored for 12 days at $8^{\circ}C$ and for 15 days at $6^{\circ}C$ without decrease in hatchability and larval survivorship, respectively.

Protein Contents During Oocyte Development and Some Characteristics of Egg-Specific Protein in Lucilia illustris (연두금파리의 난세포성숙에 따른 단백질의 변화와 난특이성단백질의 특성)

  • Lee, Jong-Jin;Man-Young Choi;Hee-Kwon Lee
    • Korean journal of applied entomology
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    • v.34 no.2
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    • pp.140-146
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    • 1995
  • Changes in protein content during oocyte development was measured and egg-specific protein was characterized from the eggs in Lucilia illustris. During normal development ovarian protein was rapidly increased at 72hr and reached maximum at 96hr after a protein meal, when the eggs were fully matured. Purified protein from the ovaries by gel filtration of DEAE-cellulose an Sephacryl S-200 was loaded on 7.5% native polyacrylamide gel electrophoresis and identified at ${R}_{f}$ 0.4 as egg-specific protein, which has a mol. wt of 110,000. A total of 13 amino acids in th egg-specific protein was identified and expecially asparagine, glutamic acid, and tyrosine were highly concentrated. Five fatty acids were also identified. It is suggested that there is a specific protein in the eggs of L. illustris except yolk protein synthesized and secreted by fat body.

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