• Title/Summary/Keyword: Low temperature

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QTL mapping of low-temperature germinability and identification of qLTG1 candidate genes in rice

  • Kim, Sun Ha;Shim, Kyu-Chan;Lee, Hyun-Sook;Le, Anh Quynh;Ahn, Sang-Nag
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.116-116
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    • 2017
  • Low-temperature is one of the environmental stress factors that affect plant growth and development and consequently limit crop productivity. The control of seed germination under low-temperature is organized by many genes which are called quantitative trait loci (QTLs). High germination rate for low-temperature is an important factor of growing rice. Previously, we identified a major QTL controlling low-temperature germinability in rice using 96 introgression lines (ILs) derived from a cross between Oryza rufipogon (Rufi) and the Korean japonica cultivar, 'Hwaseongbyeo (HS)'. A $BC_3F_7$ line (TR5) showed better low-temperature germinability than its recurrent parent. TR5 was crossed with HS to develop a segregating F2:3 populations for the target QTL. Six SSR markers polymorphic between HS and Rufi were used to screen and fine map the qLTG1. The qLTG1 on chromosome 1, which accounted for 55.5% of the total phenotypic variation, confirmed that Rufi allele enhanced the low-temperature germinability. Intervals between markers CRM16 and CRM15, four candidate genes were identified. The identified candidate genes, which are encoded by a protein of unknown function, showed their direct involvement on seed germination at low-temperature. To identify genes targeted by qLTG1, we investigated the expression profiles of these candidate genes and germination behavior of qLTG1 under different stress conditions and compared to HS, Rufi, and TR5 at $13{\pm}2^{\circ}C$ for 3 days after incubation. Furthermore, transgenic rice plants will also be developed to conduct a detailed investigation on low-temperature germinability. Hence, the QTL for low-temperature germinability would be useful in rice breeding programs especially in the development of lines possessing low-temperature germinability.

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Drying and Low Temperature Storage System of Agricultural Products using the Air to Air Heat Pump (II) - Performance of Low Temperature Storage for Apples - (히트펌프를 이용한 농산물 건조 및 저온저장 시스템 (II) - 사과의 저온저장 성능 -)

  • Kang, Y.K.;Han, C.S.;Keum, D.H.
    • Journal of Biosystems Engineering
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    • v.32 no.2 s.121
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    • pp.102-108
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    • 2007
  • Heat pump systems are recognized to be heating and cooing systems. In this study, to check the practical application possibility of heat pump systems as low temperature storage systems and get basic data, apples of a long term storage items were stored and performance of low temperature storage and quality changes of apples were evaluated. Cooling coefficient of performance of the system was from 1.1 to 1.3. Although ambient air temperature varied widely from $-13^{\circ}C$ to $29.6^{\circ}C$ during low temperature storage period from January to June, the average temperature of low temperature storage chamber was $1.1^{\circ}C$ at setting temperature of $1.5^{\circ}C$. Sucrose of apples stored by the heat pump decreased from initial sucrose of 15.4% (Brix number) to final sucrose of 14.3%. Weight loss ratio of apples was 9.7% and internal and external view of apples after low temperature storage were very satisfactory with the naked eye.

Large Scale of Ethanol Fermentation from Sweet Potato Cooked at Low and High Temperature (고구마의 저온증자 및 고온증자에 의한 공업적 규모의 주정발효)

  • 유병호;김운식;김성두;최명호;남기두;하미숙
    • Microbiology and Biotechnology Letters
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    • v.14 no.3
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    • pp.233-237
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    • 1986
  • Possibility of large scale ethanol fermentation from sweet potato were compared with low temperature and high temperature rooking. Productivity of sweet potato mash cooked at 9$0^{\circ}C$ for 120 minutes was higher than that mash cooked at 124$^{\circ}C$ for 60 minutes and also fermentation yield ai low temperature cooking was better than high temperature cooking. Low temperature cooking was successfully carried out on a large scale. In conclusion, low temperature cooking on large scale should be reduce energy consumption by approximate 30% compared with high temperature cooking.

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Electrical Characteristics of Metal/n-InGaAs Schottky Contacts Formed at Low Temperature

  • 이홍주
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.13 no.5
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    • pp.365-370
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    • 2000
  • Schottky contacts on n-In$\_$0.53//Ga$\_$0.47//As have been made by metal deposition on substrates cooled to a temperature of 77K. The current-voltage and capacitance-voltage characteristics showed that the Schottky diodes formed at low temperature had a much improved barrier height compared to those formed at room temperature. The Schottky barrier height ø$\_$B/ was found to be increased from 0.2eV to 0.6eV with Ag metal. The saturation current density of the low temperature diode was about 4 orders smaller than for the room temperature diode. A current transport mechanism dominated by thermionic emission over the barrier for the low temperature diode was found from current-voltage-temperature measurement. Deep level transient spectroscopy studies exhibited a bulk electron trap at E$\_$c/-0.23eV. The low temperature process appears to reduce metal induced surface damage and may form an MIS (metal-insulator-semiconductor)-like structure at the interface.

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Low Temperature bonding Technology for Electronic Packaging (150℃이하 저온에서의 미세 접합 기술)

  • Kim, Sun-Chul;Kim, Youngh-Ho
    • Journal of the Microelectronics and Packaging Society
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    • v.19 no.1
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    • pp.17-24
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    • 2012
  • Recently, flip chip interconnection has been increasingly used in microelectronic assemblies. The common Flip chip interconnection is formed by reflow of the solder bumps. Lead-Tin solders and Tin-based solders are most widely used for the solder bump materials. However, the flip chip interconnection using these solder materials cannot be applied to temperature-sensitive components since solder reflow is performed at relatively high temperature. Therefore the development of low temperature bonding technologies is required in these applications. A few bonding techniques at low temperature of $150^{\circ}C$ or below have been reported. They include the reflow soldering using low melting point solder bumps, the transient liquid phase bonding by inter-diffusion between two solders, and the bonding using low temperature curable adhesive. This paper reviews various low temperature bonding methods.

Molecular Identification and Fine Mapping of a Major Quantitative Trait Locus, OsGPq3 for Seed Low-Temperature Germinability in Rice

  • Nari Kim;Rahmatullah Jan;Jae-Ryoung Park;Saleem Asif;Kyung-Min Kim
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.283-283
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    • 2022
  • Abiotic stresses such as high/low temperature, drought, salinity, and submergence directly or indirectly influence the physiological status and molecular mechanisms of rice which badly affect yield. Especially, the low temperature causes harmful influences in the overall process of rice growth such as uneven germination and the establishment of seedlings, which has become one of the main limiting factors affecting rice production in the world. It is of great significance to find the candidate genes controlling low-temperature tolerance during seed germination and study their functions for breeding new rice cultivars with immense low-temperature tolerance during seed germination. In this study, 120 lines of Cheongcheong/Nagdong double haploid population were used for quantitative trait locus analysis of low-temperature germinability. The results showed significant difference in germination under low different temperature conditions. In total, 4 QTLs were detected on chromosome 3, 6, and 8. A total of 41 genes were identified from all the 4 QTLs, among them, 25 genes were selected by gene function annotation and further screened through quantitative real time polymerase chain reaction. Based on gene function annotation and level of expression under low-temperature, our study suggested OsGPq3 gene as a candidate gene controlling viviparous germination, ABA and GA signaling under low-temperature. This study will provide a theoretical basis for marker-assisted breeding.

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Effects of Temperature and Daylength on Growth and Grain Yield in Wheat (T. aestivum) (온도 및 일장조건이 소맥의 생육 및 수량에 미치는 영향)

  • Cho, C.H.;Chung, T.Y.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.24 no.2
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    • pp.35-41
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    • 1979
  • To observe the effects of temperature and photoperiod on the growth and yield of winter wheat (Triticum aestivum, L) eight varieties including Chang Kwang were tested at various treatments like longday-high temperature (24hrs-20\circ), shortdayhigh temperature (12hrs-20\circ), longday-low temperature (24hrs-14\circ) and shortday-low temperature (12hrs-14\circ). Among the traits measured, days to heading, plant height, spike length, number of spikes per hill and grain yield per hill were generally decreased at high temperature and long day treatment and increased at low temperature and shortday condition. Number of grains per spike was decreased at low temperature and short day condition while increased at longday and high temperature conditions. Grain weight was decreased significantly at high temperature and shortday while increased at low temperature and longday treatment.

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Study on Design of high Efficient Cooling System for Low Temperature Furnace in Semiconductor Processing (반도체 공정용 저온 열처리로의 고효율 냉각시스템 설계에 관한 연구)

  • Jeoung, Du-Won;Suh, Ma-Son;Kim, Kwang-Sun
    • Journal of the Semiconductor & Display Technology
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    • v.9 no.4
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    • pp.71-76
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    • 2010
  • According to recent changes in industry for semiconductor devices, a low-temperature treatment has become a necessity. These changes relate to size refinement and the development of new materials. While variation in cooling efficiency does not affect the yield when using a high-temperature treatment, uniform cooling efficiency is necessary avoid "inconsistencies/bends" in low temperature treatments. However it is difficult to increase temperature stabilization in low temperature treatments. In this paper, using CFD (Computer Fluid Dynamics), we analyze and manipulate the design and input of the low-temperature system to attempt to control for temperature variations within the quartz tube, of which airflow appears to be a predominant factor. This simulation includes variable inputs such as airflow rate, head pressure, and design manipulations in the S.C.U. (Super Cooling Unit).

Changes in the Compositions of Amino Acids in the Rice Seedlings under Low Temperature (저온처리한 벼 유식물의 아미노산 조성의 변화)

  • 문병용
    • Journal of Plant Biology
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    • v.32 no.4
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    • pp.235-245
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    • 1989
  • The contents and the compositions of total free amino acids were investigated in the rice(Oryza sativa L. cv. Chuncheong) seedlings under low temperatures. Activities of some enzymes associated with the markedly changed amino acid content were also investigaetd. Under low temperature, the contents of soluble protein and the total free amino acids increased, while the content of total nitrogen decreased. Although asparagine+glycine were the most abundant amino acid speceis in the rice seedlings at the control temeprature, low temperature treatment for 3days brought about the decrease in their amount to about 60% level of the control plants. On the other hand, alanine showed the highest increase in the content among all the free amino acids, though glutamine, proline, asprtic acid, valine and tyrosine also increased after low temperature treatment. To eludicate the decrease of asparagine+glycine level under low temperature, the activities of asparagine aminotransferase and asparaginase which metabolize asparagine were investigated in the rice seedlings under low temperature. The activity of asparaginase increased markedly, while that of asparagine aminotransferase decreased under low temperatures. Therefore, it was suggested that asparaginase metabolizes asparagine predominatly in the rice seedlings under low temperatures.

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Mapping quantitative trait loci controlling low-temperature germinability in rice

  • Nguyen, Hoang Nam;Park, In-Kyu;Yeo, Sang-Min;Yun, Yeo-Tae;Ahn, Sang-Nag
    • Korean Journal of Agricultural Science
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    • v.39 no.4
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    • pp.477-482
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    • 2012
  • Low-temperature germination is one of the major determinants for stable stand establishment in the rice direct seeding method in temperate regions and at high altitude areas. Quantitative trait loci (QTL) controlling low-temperature germinability in rice were identified using 96 introgression lines (ILs) derived from a cross between Oryza rufipogon and the Korean japonica cultivar, 'Hwaseongbyeo'. The germination rate at $15^{\circ}C$ was measured to represent low-temperature germination and used for QTL analysis. The germination rate at $15^{\circ}C$ for 7 days of Oryza rufipogon and Hwaseongbyeo was 93.3 and 28.7%, respectively, and that of progenies ranged from 0 to 48%. A linkage map was constructed using 135 simple sequence repeat (SSR) markers. Five putative QTLs associated with low-temperature germination were detected on chromosomes 1, 3, 4, 10 and 11. The QTL, qltg10 on chromosome 10 accounted for 19.2% of the total phenotypic variation for low-temperature germinability. Four additional QTL, accounted for 10.4 - 15.1% of the total phenotypic variation. The O. rufipogon alleles in all detected QTLs loci increased the low-temperature germination rate. No QTL associated with low temperature germinability has been detected near the qltg10 QTL in this study suggesting that qltg10 is a new QTL. The locus, qltg10 is of particular interest because of its independence from undesirable height and maturity effects. The DNA markers linked to the QTL for low temperature germinability would be useful in selecting lines with enhanced low temperature germinability in rice breeding program.