• Journal of Food Hygiene and Safety
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• v.27 no.1
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• pp.96-102
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• 2012

This study was designed to evaluate a repeated oral dose toxicity and immunomodulating activity of $Pulsatilla$ $koreana$ and $Artemisiae$ $annuae$ in Sprague-Dawley rats. The female rats were treated with $Pulsatilla$ $koreana$ and $Artemisiae$ $annuae$ of control group, low group (0.5 ml/kg), medium group (1 ml/kg), high group (2 ml/kg) for distilled water, intragastrically for 4 weeks, respectively. To ensure the safety of $Pulsatilla$ $koreana $ and $Artemisiae$ $annuae$ such as the following were observed and tested. We examined the body weight, the feed intake, the clinical signs, the ophthalmological test, the hematological and the serum biochemical analysis. We also observed the histopathological changes of liver and kidney in rats. Hematological results were the increase of neutrophils, lymphocytes and monocytes in the high dose group of $Pulsatilla$ $koreana$. The increase immune cells in the high dose group of $Pulsatilla$ $koreana$ might immunomodulating activity. No significant differences in body weight, feed intake, serum biochemical analysis and histopathological between control and fed group were found. In conclusion, $Pulsatilla$ $koreana$ and $Artemisiae$ $annuae$ is physiologically safe and improve immunomodulating activity.

• Horticultural Science & Technology
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• v.35 no.1
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• pp.38-45
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• 2017

We investigated the effects of rising temperatures on the photosynthesis, mineral composition, and growth of radish (Raphanus sativus var. hortensis) in a winter cropping system using a temperature gradient tunnel to predict the impact of rising global temperatures. Vegetative growth, including shoot and root fresh and dry weights, shoot length, and root length and diameter, was high under elevated temperatures (ambient $+4^{\circ}C$ and $+7^{\circ}C$) compared with ambient temperature. At elevated temperatures, the N, P, Ca, Mg, and Fe contents were high in shoots, whereas in roots, the K, Ca, Mg, and Fe contents were high and the Cu content was low. The maximum photosynthetic rates ($22.1{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ at ambient temperature $+4^{\circ}C$ and $22.9{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ at ambient temperature $+7^{\circ}C$) at elevated temperatures were more than twice that ($9.7{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) at ambient temperature, whereas the water use efficiency was lower at elevated temperatures. These results suggest that rising global temperatures will lead to increased mineral absorption and photosynthesis in radish in winter cropping systems, subsequently favoring plant growth, although the water requirements will be high.

• Horticultural Science & Technology
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• v.34 no.6
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• pp.818-829
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• 2016

This study evaluated the responses of 18 potato cultivars to three levels of salinity stress (electrical conductivity, EC: 1.0, 4.0, and $8.0dS{\cdot}m^{-1}$). Stem, leaf, root, chlorophyll, tuber yield, and proline content were investigated and statistically analyzed using analysis of variance (ANOVA) and correlations. Stem number and stem diameter were not affected by salinity, but stem length and aerial weight showed highly significant responses to salinity. Aerial weight decreased with increasing salinity levels in most cultivars, while it increased in some the cultivars 'Daejima', 'Goun', 'Haryeong', and 'LT-8'. Leaf number, leaf area index, and leaf weight were most significantly affected by salinity and the cultivar ${\times}$ salinity interaction. Root length, root weight, total chlorophyll and chlorophyll a were affected by salinity, but not by the cultivar ${\times}$ salinity interaction. The opposite trend was shown in chlorophyll b. Although there was great variability among cultivars, tuber yield decreased in all cultivars, and was most significantly influenced by salinity and the cultivar ${\times}$ salinity interaction. 'Superior', 'Kroda', 'Romana', and 'Duback' gave better tuber yields under salinity at EC 4.0 and $8.0dS{\cdot}m^{-1}$ than the cultivars with better aerial weights. Proline content was increased by salinity in all cultivars, and was more remarkable in the cultivars with better aerial weights than in cultivars such as 'Superior' and 'Kroda' with better tuber yields. Leaf number, leaf area index, leaf weight, and root length parameters were considered to be useful criteria in the evaluation of salt tolerance because of their high positive correlation with tuber yield; however, given its negative correlation with tuber yield under high salinity, proline content was not. Salinity tolerances varied greatly among potato cultivars. The low correlation between growth and yields of aerial parts under high salinity suggests that, in commercial agriculture, it might be more practical to compare relative yields to controls. Additionally, 'Superior', 'Kroda', 'Romana', and 'Duback' might be very useful cultivars to use in breeding programs to develop salinity-tolerant potatoes, as well as for sustainable potato production in saline areas.

• Journal of Animal Environmental Science
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• v.17 no.1
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• pp.39-48
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• 2011

The present survey was conducted to provide basic information on automatic milking system (AMS) in relation to purchase motive, milk yield and quality, customer satisfaction, difficulties of operation and customer suggestions, etc. Purchase motives of AMS were insufficient labor (44%), planning of dairy experience farm (25%), better performance of high yield cows (19%) and others (6%), respectively. Average cow performance after using AMS was 30.9l/d for milk yield, 3.9% for milk fat, 9,100/ml for bacterial counts. Sixty-eight percentage of respondents were very positive in response to AMS use for their successors but 18% were negative. The AMS operators were owner (44%), successor (44%), wife (6%) and company worker (6%), respectively. The most difficulty (31%) in using AMS was operating the system and complicated program manual. The rate of response to system error and breakdown was 25%. The reasons for culling cow after using AMS were mastitis (28%), reproduction failure (19%), incorrect teat placement (12%), metabolic disease (7%) and others (14%), respectively. Fifty-six percentages of the respondents made AMS maintenance contract and 44% did not. Average annual cost of the maintenance contract was 6,580,000 won. Average score for AMS satisfaction measurement (1 to 5 range) was 3.2 with decrease of labor cost 3.7, company A/S 3.6, increase of milk yield 3.2 and decrease of somatic cell count 2.8, respectively. Suggestions for the higher efficiency in using AMS were selecting cows with correct udder shape and teat placement, proper environment, capital and land, and attitude for continuous observation. Systematic consulting was highly required for AMS companies followed by low cost for AMS setup and systematization of A/S.

• Journal of Animal Environmental Science
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• v.16 no.2
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• pp.143-152
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• 2010

Conditions for artificial culture of Lemna Paucicostata and its nutritional values were examined in this study. Lemna P. was cultured using artificial wastewater and a bioreactor (total volume $2,630\;cm^3$, working volume $2,240\;cm^3$) was operated at conditions of 6,250 lux and $28^{\circ}C$. Water flow affected the growth of Lemna P.: growth rate was very high (more than $1.1\;d^{-1}$) at a condition of no-water movement, but it was very low (less than $0.15\;d^{-1}$) when water moved slowly. The growth of Lemna P. was higher in $16h\;d^{-1}$ light cycle than in Sand $24h\;d^{-1}$, and it was also severely affected by the initial $NH_4$-N levels of wastewater. The growth rate of Lemna P. was high in lower $NH_4$-N level, indicating that the growth rate is in inverse proportion to $NH_4$-N concentration in wastewater. However, the contents of crude protein (CP) of Lemna P. were proportional to the initial $NH_4$-N concentration. The CP contents of Lemna P. cultured at 2, 10, 50 and 100 $NH_4$-N mg $L^{-1}$ was 18, 24, 37, 43%, respectively, showing the Lemna P. cultured at 50 and $100\;mg\;L^{-1}$ had similar protein contents to linseed (CP 35%), cottonseed (CP 38%) and soybean (CP 45%). Fat, protein, fiber, NDF and ADF contents of Lemna P. harvested at conditions of $16h\;d^{-1}$ light cycle and less than $2\;mg\;L^{-1}$ of $NH_4$-N level was 2.8, 18, 27, 20, 41 and 65.7%, respectively. Since the growth rate of Lemna P. was very high (more than $1.1\;d^{-1}$) at those conditions, it was convinced that mass production of valuable protein and fiber sources are feasible. In particular, since the Lemna P. has unsaturated fatty acids found mainly in animal fat as well as beneficial fatty acids to health such as C18:ln9c, C18:2n6c, C20:5n3 and C22:2, the Lemna P. biomass would be a highly valuable alternative feed source to grains.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.34 no.1
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• pp.43-52
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• 2016

Due to the characteristics of microwave signals, Radar satellite image has been used for flood detection without weather and time influence. The more methods of flood detection were developed, the more detection rate of flood area has been increased. Since flood causes a lot of damages, flooded area should be distinguished from non flooded area. Also, the detection of flood area should be accurate. Therefore, not only image resolution but also the filtering process is critical to minimize resolution degradation. Although a resolution of radar images become better as technology develops, there were a limited focused on a highly suitable filtering methods for flood detection. Thus, the purpose of this study is to find out the most appropriate filtering method for flood detection by comparing three filtering methods: Lee filter, Frost filter and NL-means filter. Therefore, to compare the filters to detect floods, each filters are applied to the radar image. Comparison was drawn among filtered images. Then, the flood map, results of filtered images are compared in that order. As a result, Frost and NL-means filter are more effective in removing the speckle noise compared to Lee filter. In case of Frost filter, resolution degradation occurred severly during removal of the noise. In case of NL-means filter, shadow effect which could be one of the main reasons that causes false detection were not eliminated comparing to other filters. Nevertheless, result of NL-means filter shows the best detection rate because the number of shadow pixels is relatively low in entire image. Kappa coefficient is scored 0.81 for NL-means filtered image and 0.55, 0.64 and 0.74 follows for non filtered image, Lee filtered image and Frost filtered image respectively. Also, in the process of NL-means filter, speckle noise could be removed without resolution degradation. Accordingly, flooded area could be distinguished effectively from other area in NL-means filtered image.

• Journal of The Korean Society of Grassland and Forage Science
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• v.37 no.4
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• pp.337-344
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• 2017

This experiment was carried out to determine the effect of organic fertilizer and seed mixture on productivity, botanical composition and forage quality in middle areas(Geumsan, 250m sea level) pasture. Total six experimental pastures (orchardgrass, timothy and tall fescue based seed mixture and with or without organic fertilizer) were established in autumn, 2014 and evaluated productivity and agronomic characteristics from 2015 to 2016. Plant heigh was higher in orchardgrass based mixture and orchardgrass and tall fescue based mixture were higher in $2^{nd}$ harvest. There was not found the significant difference among treatment(P<0.05) in dry matter (DM) content. Botanical composition in $1^{st}$ harvest was higher weed portion in orchardgrass based mixture and legumes was disappeared at $3^{rd}$ harvest in all treatments. Fresh and dry matter yield was higher in $2^{nd}$ year than $1^{st}$ year. In $1^{st}$ year, orchardgrass based mixture with organic fertilizer plot was the highest(P<0.05) DM yield, but tall fescue based mixture with organic fertilizer was the highest in $2^{nd}$ year. Content of crude protein (CP) was the highest in tall fescue based mixture and Timothy based mixture showed low in ADF (acid detergent fiber) and NDF (neutral detergent fiber) content. But the content if in virto dry matter digestibility was higher than others. In $2^{nd}$ year, the nutritive value of $2^{nd}$ and $4^{th}$ harvested grasses were higher and average RFV (relative feed value) value was the highest at $4^{th}$ harvest. According to this result, orchardgrass and tall fescue based mixture would be recommendable for farm who considering productivity and timothy based mixture would be recommendable for considering forage quality in middle areas of Korea.

• Horticultural Science & Technology
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• v.18 no.6
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• pp.834-838
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• 2000

As an endeavor to establish a micropropagation system for Epimedium koreanum Nakai., this study was carried out to define methods to disinfect its explants and media for callus induction, proliferation and plant regeneration. The lowest infection rates by fungi or bacteria on apical and axillary bud explants of rhizome were observed when they were immerged in 0.3% NaOCl solution for 20 min after soaked in 0.1% $AgNO_3$ solution for 30 min, but leaf explants were seldom infected with fungi or bacteria by this disinfectant method. The highest rate of plantlet formation was obtained from the explants disinfected in 0.3% NaOCl solution for 20 min after soaked in 0.1% $AgNO_3$ solution for 60 min for tip buds and in 0.1 % $AgNO_3$ solution for 30 min for axillary buds of rhizome. Induction rate of callus was the highest from the explants disinfectd in 0.3% NaOCl solution for 20 min after soaked in 0.2% $AgNO_3$ solution for 15 min. Callus growth was proper in a modified 1/2 MS medium including half strength of $NH_4NO_3$ with $0.02-0.2mg{\cdot}L^{-1}$ BA and $2.0mg{\cdot}L^{-1}$ NAA. Low rate of plantlet regeneration was obtained in 1/2 UM or 1/2 White medium with $2.0mg{\cdot}L^{-1}$ BA and $0.2mg{\cdot}L^{-1}$ AA.

• Analytical Science and Technology
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• v.23 no.2
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• pp.187-195
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• 2010

To assess health risk for the intake among residents after the Hebei Spirit oil spill, 16 Polycyclic Aromatic Hydrocarbons (PAHs) in seafood samples from oil contaminated bay were determined by Gas Chromatography-Mass Spectrometry (GC-MSD) and samples were personally collected and purchased by residents. Samples were hydrolyzed with KOH and extracted with methylene chloride. The extracted solution were cleaned up using silica/florisil column and 16 PAHs were eluted by methylene chloride : n-hexane (1:9) mixture and determined by GC-MSD in Selected Ion Monitoring (SIM) mode. The mean recoveries for 16 PAHs ranged from 79% to 85%. The 16 PAHs levels in 126 samples ranged from 0.17 to $6.04\;{\mu}g$/kg and the TEQBaP (Toxic EQuivalents) levels in 126 samples were calculated using benzo(a)pyrene toxic equivalency factor for individual 16 PAHs and ranged from 0 to $0.91\;{\mu}gTEQ$/kg. The average Benzo(a)pyrene dietary exposure of residents was $5.5{\times}10^{-8}\;mg/kg$ bw/day and the average PAHs chronic dietary exposure was $1.3{\times}10^{-5}\;mg$ TEQ/kg bw/day. The margin of exposure (MOE) and the excess cancer risk and were $1.8{\times}10^6$ and $9.8{\times}10^{-8}$, respectively. Therefore, the assessment result was considered as low concern for health risk.

• Korean Journal of Environmental Agriculture
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• v.28 no.2
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• pp.158-164
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• 2009

Biosorption uses adsorbents derived from non-living biomass and removes toxic metals from industrial wastewater. The objective of this research was to evaluate the potential of low cost biosorbents to remove heavy metal ions (Cd, Cu, Pb and Zn) from aqueous solutions using chemically modified rice husk and saw dust (Pseudotsuga menziesi, Quercus, Populus). Batch-type adsorption experiments were carried out using rice husk and saw dust treated with NaOH and/or tartaric acid in artificial wastewater 100 mg metal/L). The experimental results showed that the adsorption specificity of each biosorbent was Pb > Cu > Cd > Zn irrespective of the types of biosorbents. The adsorption capacity of Pb and Cu onto NaOH-treated sawdust was increased 2${\sim}$3 times compared to the untreated one. In addition, the tartaric acid treatment increased the adsorption capacity of rice husk for Zn and Cd approximately 5${\sim}$10 fold compared to the untreated one. Surface conditions and changes in functional groups by chemical modification of each biosorbent were confirmed by SEM and FT-IR. Overall, the results show that chemical modification increases the metal removal capacity of rice bran and sawdust.