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http://dx.doi.org/10.13103/JFHS.2012.27.1.096

Evaluate a Repeated Oral Dose Toxicity and Immunomodulating Activity of $Pulsatilla$ $koreana$ and $Artemisiae$ $annuae$ in Sprague-Dawley Rats  

An, In-Jung (Department of Companion and Laboratory Animal Science, Kongju National University)
Kwon, Jung-Ki (Department of Companion and Laboratory Animal Science, Kongju National University)
Lee, Jin-Seok (Department of Companion and Laboratory Animal Science, Kongju National University)
Lee, Seung-Ho (Department of Companion and Laboratory Animal Science, Kongju National University)
Park, Young-Seok (Department of Companion and Laboratory Animal Science, Kongju National University)
Park, Byung-Kwon (Department of Companion and Laboratory Animal Science, Kongju National University)
Kim, Sang-Ki (Department of Companion and Laboratory Animal Science, Kongju National University)
Kim, Byeong-Soo (Department of Companion and Laboratory Animal Science, Kongju National University)
Cho, Sung-Dae (School of dentistry, Institute of Oral Bioscience, Chonbuk National University)
Choi, Chang-Sun (School of Food Science and Technology, Chung-Ang University)
Lee, Byoung-Hee (National Institute of Biological Resources)
Kang, Byeong-Kon (Shinil Biogen)
Jung, Ji-Youn (Legume Bio-Resource Center of Green Manure (LBRCGM), Kongju National University)
Publication Information
Journal of Food Hygiene and Safety / v.27, no.1, 2012 , pp. 96-102 More about this Journal
Abstract
This study was designed to evaluate a repeated oral dose toxicity and immunomodulating activity of $Pulsatilla$ $koreana$ and $Artemisiae$ $annuae$ in Sprague-Dawley rats. The female rats were treated with $Pulsatilla$ $koreana$ and $Artemisiae$ $annuae$ of control group, low group (0.5 ml/kg), medium group (1 ml/kg), high group (2 ml/kg) for distilled water, intragastrically for 4 weeks, respectively. To ensure the safety of $Pulsatilla$ $koreana $ and $Artemisiae$ $annuae$ such as the following were observed and tested. We examined the body weight, the feed intake, the clinical signs, the ophthalmological test, the hematological and the serum biochemical analysis. We also observed the histopathological changes of liver and kidney in rats. Hematological results were the increase of neutrophils, lymphocytes and monocytes in the high dose group of $Pulsatilla$ $koreana$. The increase immune cells in the high dose group of $Pulsatilla$ $koreana$ might immunomodulating activity. No significant differences in body weight, feed intake, serum biochemical analysis and histopathological between control and fed group were found. In conclusion, $Pulsatilla$ $koreana$ and $Artemisiae$ $annuae$ is physiologically safe and improve immunomodulating activity.
Keywords
Pulsatilla koreana; Artemisiae annuae; Toxicity;
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