• Journal of the Society of Naval Architects of Korea
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• v.48 no.3
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• pp.189-199
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• 2011

Austenite stainless steel(ASS), aluminum alloy and nickel steel alloy are the most widely used in many cryogenic applications due to superior mechanical properties at low temperature. The Face-Centered Cubic(FCC) and Hexagonal Close-Packed(HCP) materials are used for the primary and secondary insulation barrier of Liquefied Natural Gas(LNG) carrier tank and various kinds of LNG applications currently. In this study, tensile tests of ASS, aluminum alloy and nickel steel alloy were carried out for the acquisition of quantitative mechanical properties under the cryogenic environment. The range of thermal condition was room temperature to $-163^{\circ}C$ and strain rate range was 0.00016/s to 0.01/s considering the dependencies of temperatures and strain rates. The comprehensive test data were analyzed in terms of the characteristics of mechanical behavior for the development of constitutive equation and its application.

• Journal of the Korean Wood Science and Technology
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• v.51 no.4
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• pp.270-282
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• 2023

Lower termites need symbiotic microbes for cellulose digestion. Elizabethkingia miricola strain BM10 has been proposed as a symbiotic microbe that assists in low-temperature digestion and metabolism of Reticulitermes speratus KMT1, a termite on Bukhan Mountain, Seoul, Korea. In E. miricola strain BM10, β-glucosidase genes expressed at 10℃ were identified, and the psychrophilic enzymatic characteristic was confirmed by heterogeneously expressed proteins. Crude β-glucosidase in the culture broth of E. miricola strain BM10 showed specific enzymatic properties, and its substrate affinity was 4.69 times higher than that of Cellic CTec2. Among the genes proposed as β-glucosidase, two genes, bglB_1 and bglA_2, whose gene expression was more than doubled at 10℃ than at 30℃, were identified. They were heterogeneously expressed in Escherichia coli and identified as psychrophilic enzymes with an optimal reaction temperature of about 20℃-25℃. In this study, E. miricola strain BM10, a symbiotic bacterium of lower termites, produced psychrophilic β-glucosidases that contribute to the spread of the low-temperature habitat of a lower termite, R. speratus KMT1.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.21 no.2
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• pp.199-208
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• 1997

A local strain approach was applied to an external single and double grooved C-shaped specimen in order to evaluate and predict the fatigue crack initiation life by using low cycle fatigue properties. The low cycle fatigue properties were determined from the strain-controlled fatigue tests using smooth cylindrical axial specimens. Fatigue crack initiation life was evaluated by a life prediction software, FALIPS, based on the local strain approach. The fatigue life was significantly influenced by the mean stress, and SWT parameter represented the fatigue life effectively. The predicted fatigue crack initiation life was then compared to the experimental fatigue life evaluated from the C-shaped fatigue test specimens. A good correlation was found between the experimental and predicted fatigue lives within factors of 2 and 4 for the single and double grooved C-shaped specimens respectively. Also, experimental fatigue life of the double grooved specimen was 10-12 times longer than that of the single grooved specimen.

• Journal of the Korean Society for Heat Treatment
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• v.32 no.1
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• pp.12-16
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• 2019

In this study, strain-controlled low cycle fatigue test for hot rolled STS304 steel was carried out at $400^{\circ}C$ and $600^{\circ}C$, respectively. High temperature fatigue test was done using an electric furnace attached on the hydraulic fatigue test machine. The results of this study show that STS304 hot rolled steel has excellent static strength and fatigue characteristics. The hysteresis loop at half life was obtained in order to calculate the elastic and plastic strain. Also, Relationship between strain amplitude and fatigue life was examined in order to predict the low cycle fatigue life of STS304 steel by Coffin-Manson equation.

• Proceedings of the KSME Conference
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• 2007.05a
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• pp.17-22
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• 2007

Low-cycle environmental fatigue tests of cast austenitic stainless steel CF8M at the condition of fatigue strain rate 0.04%/sec were conducted at the pressure and temperature, 15MPa, $315^{\circ}C$ of a operating pressurized water reactor. The used test rig was limited to install an extensometer at the gauge length of the cylindrical fatigue specimen inside the small autoclave. So the magnet type LVDT's were used to measure the fatigue displacement at the specimen shoulders inside the high temperature and high pressure water autoclave. However, the displacement and strain measured at the specimen shoulders is different from the one at the gauge length for the geometry and the cyclic strain hardening effect. FEM calculated the displacement and the strain of the gauge length from the data measured at the shoulders. Tensile test properties in elastic and plastic behavior of CF8M material were used in the FEM analysis. A series of low cycle fatigue tests simulating the cyclic strain hardening effect verified that the FEM calculation was well agreed with the simulated tests. The process and method developed in this study would be so useful to produce reliable environmental fatigue curves of CF8M stainless steel in pressurized water reactors.

• Journal of the Korean Applied Science and Technology
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• v.39 no.5
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• pp.632-643
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• 2022

Although probiotics have been shown to improve health when consumed, recent studies have reported that they can cause unwanted side effects due to bacterial-human interactions. Therefore, the importance of prebiotics that can form beneficial microbiome in the gut has been emphasized. This study isolated and identified bacteria capable of producing biopoymer as a candidate prebiotic from traditional fermented foods. The isolated and identified strain was named WCYSK01 (Wissella sp. strain YSK01). The composition of the medium for culturing this strain was prepared by dissolving 3 g K₂HPO₄, 0.2 g MgSO₄, 0.05 g CaCl₂, 0.1 g NaCl in 1 L of distilled water. The LMBP(low molecular weight biopoymers) produced when fermentation was performed with sucrose and maltose as substrates were mainly consisted of DP3 (degree of polymer; isomaltotriose), DP4 (isomaltotetraose), DP5 (isomaltopentaose), and DP6 (isomaltoheptaose). The optimization of LMBP (low molecular weight of biopolymer) production was performed using the response surface methodology. The fermentation process temperature range of 18 to 32℃, the fermentation medium pH in the range of 5.1 to 7.9. The yield of LMBP production by the strain was found to be significantly affected by q fermentation temperature and pH. The optimal fermentation conditions were found at the normal point, and the production yield was more than 75% at pH 7.5 and temperature of 23℃.

• Journal of Microbiology and Biotechnology
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• v.7 no.6
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• pp.397-401
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• 1997

The present research program was conducted to characterize a strain of actinomycetes producing an anti methicillin-resistant Staphylococcus aureus (MRSA) antibiotic. Soil samples were collected from various sites in Korea and a number of actinomycetes were isolated from the soil samples by applying selective agar for actinomycetes. Among over 400 isolates, a strain (AMLK-135) producing anti-MRSA antibiotic against S. aureus TK 784 was selected. According to the morphological and physiological characteristics, the strain AMLK-135 was confirmed to belong to the genus Streptomyces. From the results of species identification with the TAXON program, the strain AMLK-135 was shown to belong to major cluster 5 (Streptomyces exfoliatus), but it had a low simple matching coefficient ($S_{SM}$ SM/) value to member organisms of major cluster 5. Percentage ($\%$) of strain further away of the strain AMLK-135 was low (1.9400) and it was placed further away than the outer-most members in major cluster 5. Therefore, the strain AMLK-135 was identified as a new species of the genus Streptomyces.

• Transactions of the Korean Society of Automotive Engineers
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• v.17 no.4
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• pp.118-125
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• 2009

Bumper is designed to protect the automotive frame without damage at low velocity. Expanded polypropylene (EPP) foam is used in the bumper as an energy absorbing material. In order to exactly predict the energy absorbing performance of the foam material under impact loading condition, it is important to use high strain rate material properties. In this study, a new apparatus for dynamic compression tests was developed to investigate the high strain rate behavior of EPP foams. Three kinds of EPP foams which have different expansion ratios were tested to investigate the quasi-static and dynamic compression behavior. Quasi-static compressions were performed at low strain rates of 0.001/s, 0.1/s and 1/s. The dynamic compressions were carried out at high strain rates of 50/s and 100/s with the developed apparatus. It was observed that the EPP foam has significant strain rate effect as compared to quasi-static behavior.

• Proceedings of the Korea Concrete Institute Conference
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• 2003.05a
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• pp.53-58
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• 2003

Many researchers have rigorously studied the nonlinear behavior of stress-strain relationship of concrete using mathematical curves. Most of model equations for stress-strain relationship, however, have been focused on old age concrete, and were not able to adequately represent the behavior of concrete at an early age. A wide understanding on the behavior of concrete from early age to old age is very important in evaluating the durability and service life of concrete structures. In previous study by authors of this paper, a stress-strain model equation for low- and medium-strength concretes was suggested. In this paper, to extend the application region of compressive stress-strain curve to high-strength concrete, an analytical research was performed. An analytical expression of stress-strain curve with strength and age was developed using regression analyses on the experimental results. For the verification of the proposed model equation, it was compared to the experimental data. The result showed that the proposed model equation was not only compatible with the experimental data quite satisfactorily but also describing well the effect of strength and age on stress-strain curve.

• Computers and Concrete
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• v.20 no.5
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• pp.595-603
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• 2017

Piezoceramic transducers have been widely used in the health monitoring of civil structures. However, in most cases, they are used as sensors either to measure strain or receive stress waves. This paper proposes a method of using piezoelectric transducers as strain gauges and acoustic emission (AE) sensors simultaneously. The signals received by piezoceramic transducers are decomposed into different frequency components for various analysis purposes. The low-frequency signals are used to measure strain, whereas the high-frequency signals are used as acoustic emission signal associated with local damage. The b-value theory is used to process the AE signal in piezoceramic transducers. The proposed method was applied in the bending failure experiments of two reinforced concrete beams to verify its feasibility. The results showed that the extracted low-frequency signals from the piezoceramic transducers had good agreement with that from the strain gauge, and the processed high-frequency signal from piezoceramic transducers as AE could indicate the local damage to concrete. The experimental results verified the feasibly of structural health monitoring using piezoceramic transducers as strain gauges and AE sensors simultaneously, which can advance their application in civil engineering.