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http://dx.doi.org/10.12925/jkocs.2022.39.5.632

Characterization and Production of Low Molecular Weight of Biopolymer by Weisella sp. strain YSK01 Isolated from Traditional Fermented Foods  

Cho, Hyun Ah (Department of Industry Convergence Engineering, Soonchunhyang University)
Kim, Nam Chul (Research Institute of Natural Well Food Company)
Yoo, Sun Kyun (Research Institute of Natural Well Food Company)
Publication Information
Journal of the Korean Applied Science and Technology / v.39, no.5, 2022 , pp. 632-643 More about this Journal
Abstract
Although probiotics have been shown to improve health when consumed, recent studies have reported that they can cause unwanted side effects due to bacterial-human interactions. Therefore, the importance of prebiotics that can form beneficial microbiome in the gut has been emphasized. This study isolated and identified bacteria capable of producing biopoymer as a candidate prebiotic from traditional fermented foods. The isolated and identified strain was named WCYSK01 (Wissella sp. strain YSK01). The composition of the medium for culturing this strain was prepared by dissolving 3 g K2HPO4, 0.2 g MgSO4, 0.05 g CaCl2, 0.1 g NaCl in 1 L of distilled water. The LMBP(low molecular weight biopoymers) produced when fermentation was performed with sucrose and maltose as substrates were mainly consisted of DP3 (degree of polymer; isomaltotriose), DP4 (isomaltotetraose), DP5 (isomaltopentaose), and DP6 (isomaltoheptaose). The optimization of LMBP (low molecular weight of biopolymer) production was performed using the response surface methodology. The fermentation process temperature range of 18 to 32℃, the fermentation medium pH in the range of 5.1 to 7.9. The yield of LMBP production by the strain was found to be significantly affected by q fermentation temperature and pH. The optimal fermentation conditions were found at the normal point, and the production yield was more than 75% at pH 7.5 and temperature of 23℃.
Keywords
Weissella; prebiotic; fermentation; low molecular weight biopolymer; response surface methodology;
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