• Microbiology and Biotechnology Letters
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• v.42 no.4
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• pp.339-346
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• 2014

A bacterial strain was isolated from homemade doenjang (Korean fermented soybean paste) as a producer of the extracellular ${\beta}$-galactosidase, capable of hydrolyzing lactose to liberate galactose and glucose residues. The isolate YB-1414 has been identified as Bacillus licheniformis on the basis of its 16S rDNA sequence, morphology and biochemical properties. The production of ${\beta}$-galactosidase by B. licheniformis YB-1414 reached maximum levels of 6.2 U/ml in culture medium containing wheat bran (1%) and yeast extract (2.5%) as carbon and nitrogen sources, respectively. Particularly, the insoluble fraction was more effective for ${\beta}$-galactosidase production than the soluble extract of wheat bran. The enzyme exhibited maximum activity for hydrolysis of para-nitrophenyl-${\beta}$-D-galactopyranoside (pNP-${\beta}Gal$) under reaction conditions of pH 6.0 and $55-60^{\circ}C$. Its hydrolyzing activity for pNP-${\beta}Gal$ was drastically decreased by the addition of low concentrations of galactose, but only slightly decreased by glucose, with 85% of maximal activity in the presence of 400 mM glucose.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.6
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• pp.845-848
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• 2016

The effects of temperature and irradiance on the growth of Ulva prolifera O.F. $M{\ddot{u}ller$ (Chlorophyta), which has been used commercially as food in Korea, were examined in laboratory culture to conserve the strain. Experiments were conducted under combined factors of temperatures of 5, 10, 15, 20 and $25^{\circ}C$ and irradiances of 30, 50, 80 and $100{\mu}mol\;m^{-2}s^{-1}$. The maximum growth of the U. prolifera germlings was observed at $20^{\circ}C$ and $80{\mu}mol\;m^{-2}s^{-1}$, and the minimum growth was recorded at $5^{\circ}C$ and $100{\mu}mol\;m^{-2}s^{-1}$. Considering these results, the species appears to survive under wide ranges of temperature and irradiance, although growth is inhibited by high irradiances of over $100{\mu}mol\;m^{-2}s^{-1}$. This appears to be due to the in situ habitat niche of Ulva prolifera, which is the middle to lower intertidal zone. In conclusion, optimal conditions for the long-term conservation of Ulva prolifera can be established under relatively low temperatures ($5^{\circ}C$) and high irradiance ($100{\mu}mol\;m^{-2}s^{-1}$), while the optimal conditions for mass production are $20^{\circ}C$ and $80{\mu}mol\;m^{-2}s^{-1}$.

• Composites Research
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• v.16 no.6
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• pp.41-47
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• 2003

Composites are widely used for aircraft, satellite and other structures due to its good mechanical and thermal characteristics such as low coefficient of thermal expansion(CTE), heat-resistance, high specific stiffness and specific strength. In order to use composites under condition of high temperature, however, material properties of composites at high temperatures must be measured and verified. In this paper, material properties of T700/Epoxy were measured through tension tests of composite specimens with an embedded FBG sensor in the thermal chamber at the temperatures of RT, $100^{\circ}$, $200^{\circ}$, $300^{\circ}$, $300^{\circ}$. Through the pre-test of an embedded optical fiber, we confirmed the embedding effects of an optical fiber on material properties of the composites. Two kinds of specimens of which stacking sequences are [0/｛0｝/0]$_{T}$. and [$90_2$/｛0｝/$90_2$]. were fabricated. From the experimental results, material property changes of composites were successfully shown according to temperatures and we confirmed that fiber Bragg grating sensor is very appropriate to strain measurement of composites under high temperature.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.25 no.4
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• pp.160-165
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• 2015

Lead-free piezoelectric materials have been actively studied to substitute for conventional PZT based solid solution, $Pb(Zr_xTi_{1-x}O_3)$, which occurs unavoidable PbO during the sintering process. Among them, Bismuth Sodium Titanate, $Na_{0.5}Bi_{0.5}TiO_3$ (abbreviated as NBT) based solid solution is attracted for the one of excellent candidates which shows the strong ferroelectricity, Curie temperature (Tc), remnant polarization (Pr) and coercive field (Ec). Especially, the solid solution of rhombohedral phase NBT with tetragonal perovskite phase has a rhombohedral - tetragonal morphotropic phase boundary. Modified NBT with tetragonal perovskite at the region of MPB can be applied for high frequency ultrasonic application because of not only its low permittivity, high electrocoupling factor and high mechanical strength, but also effective piezoelectric activity by poling. In this study, solid state ceramic processing of NBT and modified NBT, $(Na_{0.5}Bi_{0.5})_{0.93}Ba_{0.7}TiO_3$ (abbreviated as NBT-7BT), at the region of MPB using 7 % $BaTiO_3$ as a tetragonal perovskite was introduced and the structure between NBT and NBT-7BT were analyzed using rietveld refinement. Also, the ferroelectric and piezoelectric properties of NBT-7BT such as permittivity, piezoelectric constant, polarization hysteresis and strain hysteresis loop were compared with those of pure NBT.

• Korean Journal of Veterinary Service
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• v.15 no.1
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• pp.32-45
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• 1992

In order to establish and enzyme-linked immunosorbent assay to ILTV, field virus strain of ILTV was propagated in chorioallantoic membrane of the embryonated eggs. purified and used as antigen. The antisera selected from the field samples and immunized chickens based on serum neutralization test were used as the standard positive and negative sera in all tests. It was found that optimal antigen concentration was $2{\mu}g$ of protein per well and a 1 : 100 dilution of standard serum showed low background optical density with negative serum and high P/N values of positive sera. A 1 : 500 dilution of the rabbit anti-chicken IgG peroxidase conjugate produced a high P/N values and thirty minutes was chosen as suitable time to read the optical density of the enzyme substrate reaction and optical density was consistent during the 16 hours after stopper was treated. When coated antigen was kept on microplate for varying time up to 16 hours at $4^{\circ}C$ or $37^{\circ}C,$ no significant difference was observed between the treatment. The coated antigen could be kept without change of antigenicity for at least one month at $-70^{\circ}C,\; -20^{\circ}C,\; 4^{\circ}C$ and room temperature. When blocking buffer contanining bovine serum albumin was mixed directly with conjugate and serum at 10% level induced higher P/N values compared to blocking antigen coated microplate with the same blocking buffer. The coefficience of correlation between ELISA and SN test was 0.577. When antibody response of chickens, vaccinated with ILTV, was examined by ELISA and SN test, antibody rising and decay pattern between the two test was similar until 11 weeks of age. However 12 weeks onward antibody titer checked on by SN test was slightly lower than that tested by ELISA.

• Advanced Composite Materials
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• v.17 no.3
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• pp.235-246
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• 2008

This paper is concerned with geotextiles bonded chemically with geogrid to form a geocomposite. Geotextiles, thermally bonded and non-woven, play an important role as a separator. Also, this study investigates the resistance to the application environment of geotextile composites. Here, numerous tests have been performed and it was revealed from experimental results that thermally bonded geotextile in geosynthetic composites showed superior characteristics to that manufactured from needle punched non-woven method in terms of tensile strength, tensile strain and high separation performance. It was noted from experiments that the geotextile prepared for separation purpose and manufactured in a thermal bonding method showed relatively low permittivity so that it could be used as a smooth separator. In addition, PVA geotextile/HDPE geomembrane composites were designed and manufactured to investigate the waste landfill related properties. Numerous experiments have been performed and experimental results were summarized to evaluate practical applicability of PVA geotextile/HDPE geomembrane composites. Among the properties of proposed geomembrane composites, evaluation has been focused on the investigation of mechanical properties, AOS (apparent opening size), permittivity and ultraviolet stability.

• Physical Therapy Korea
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• v.27 no.1
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• pp.30-37
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• 2020

Background: To prevent or reduce the risk of strain injury, various approaches, including stretching techniques are currently being used. The effect of proprioceptive neuromuscular facilitation (PNF) and static stretching on flexibility has been demonstrated; however, it is not clear which one is superior. Objects: This study aimed to evaluate the differences between the effects of PNF and static stretching performed at various intensities on muscle flexibility. Methods: The maximum voluntary isometric contraction (MVIC) of the hamstrings using the PNF stretching technique was performed in the P100 group, while 70% of the MVIC was performed in the P70 group. The MVIC value obtained during the PNF stretching in both groups was used as a reference for setting the intensity of static stretching. Static stretching was performed at 130% (S130), 100% (S100), and 70% of the MVIC (S70). The active knee extension (AKE) values, defined as the knee flexion angle were measured before stretching (baseline), immediately after stretching (post), and at 3 minutes, 6 minutes, and 15 minutes. Results: PNF stretching produce a greater improvement in flexibility compared with static stretching. Specifically, the ΔAKE was significantly higher in the S100 and S70 groups than in the P100 group at Post. In the comparison of ΔAKE over time in each group, the ΔAKE at Post showed a significant decrease compared to the value at Baseline in the S130 group; however, no significant difference was observed at 6 minutes while a significant increase was noted at 15 minutes. Conclusion: This study found that PNF stretching is more effective than static stretching with respect to increasing and maintaining the flexibility of muscles. In addition, the increase in flexibility at maximal intensity was similar to that observed at submaximal intensity during both PNF and static stretching.

• Journal of Environmental Science International
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• v.6 no.3
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• pp.249-258
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• 1997

Mercury- and cadmium-resistant bacteria were Isolated from an Industrial complex wastewater of Taejon area. All of them were motile, gram negative rods. In the results of physicochemical test and VITEK card test. HM1 was Identified with Achetobacter cd- coaceucus, CM3 was Identified 65 Commonas acidovorns, HM2, HM3, CMI , and CM4 were Pseudomonas sp., but HM4 and CM2 were unidenteed. They were tested for subceptlbility to 14 heavy metals. Mercury-resistant bacteria(HM1, HM2, HM3, and HM4) were sensitive to low concentration(100~400ppm) of $Cd^{2+}$, $Co^{2+}$. $Zn^{2+}$, and Ni2+, while cadmium-resistant bacteria(CM1, CM2, CM3, and CM4) showed resistance up to the high concentration(600~ 1, 200ppm) of these metal loons. As a result of resistance spectrum test of mercury-resistant bacteria, HM1 was broad-spectrum strain, HM2. HM3, and HM4 were narrow-spectrum stratas. Transmission electron microscopic examination of cell wall of HM1 culture grown with and without 100ppm of $HgCl_2$ showed remarkably merphological abnormalities. In the result of atomic absorption spectrometric analysis of cadmium-resistant bacteria grown at 200ppm of $CdCl_2$ for 6h, all of them accumulated cadrnium(14ppm~57ppm) In cell. In cadinium-resistant bacteria, CM1, CM2, and CM4 were spared from the Inhibitory effect of $Cd^{2+}$ by the addition of $Mn^{2+}$, CM4 were also spared from the Inhibitory effect of $Cd^{2+}$ by the addition of $Mn^{2+}$ as well as $Zn^{2+}$.

• Journal of the Korean Geotechnical Society
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• v.21 no.7
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• pp.5-12
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• 2005

In order to investigate the strength and deformation anisotropy of compacted decomposed granite soils, a series of unsaturated-drained triaxial compression tests were performed. The sample used in the study was decomposed granite soil from Shimonoseki in Yamaguchi prefecture. The sample had three different angles of the axial (major principal) direction to the sedimentation plane (compaction plane), 0, 45 and 90 degrees. The compression strain of specimens subjected to isotropic compression was strongly influenced by the sedimentation angle. In addition, the time dependence was independent of the sedimentation angle in relation to the deformation behavior during the secondary compression process. The effect of the sedimentation angle on the triaxial compression strength and deformation was clear with low confining stress. Moreover, it was recognized that although the sedimentation angle and preparation methods were different, the dilatancy rate was relative to the increment of strength due to dilatancy. Therefore, it may be concluded that the compacted specimen has anisotropic mechanical properties similar to those of sand with initial fabric anisotropy.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2008.06a
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• pp.144-144
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• 2008

Blue light emitting diode structures consisting of the InGaN/GaN multiple quantum wells were grown by metalorganic chemical vapor deposition at different growth temperatures for the p-GaN contact layers and the influence of growth temperature on the emission and microstructural properties was investigated. The I-V and electroluminescence measurements showed that the sample with a p-GaN layer grown at $1084^{\circ}C$ had a lower electrical turn-on voltage and series resistance, andenhanced output power despite the low photoluminescence intensity. Transmission electron microscopy (TEM) revealed that the intense electro luminescence was due to the formation of a p-GaN layer with an even distribution of Mg dopants, which was confirmed by TEM image contrast and strain evaluations. These results suggest that the growth temperature should be optimized carefully to ensurethe homogeneous distribution of Mg as well as the total Mg contents in the growth of the p-type layer.