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Vitellogenin ELISA System Based on Monoclonal and Polyclonal Antibodies against Vitellin of Floating Goby (Chaenogobius annularis) (꾹저구(Chaenogobius annularis)의 난황단백질에 대한 다클론 항체와 단글론 항체을 이용한 Vitellogenin ELISA System)

  • Kang, Bong-Jung;Jung, Jee-Hyun;Lee, Je-Yong;Kim, Myung-Hee;Han, Chang-Hee
    • Development and Reproduction
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    • v.9 no.2
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    • pp.135-142
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    • 2005
  • Vitellogenins(VTGs) are the precursor of egg-yolk proteins in most oviparous species from invertebrates to vertebrates. In oviparose vertebrates, VTGs are synthesized in the liver and transported through the blood to oocytes. In female fish, concentrations of plasma VTG increase rapidly at onset of vitellogenesis in the normal reproductive cycle. Male fishes also possess the gene for VTG, but plasma concentrations of the protein typically remain small, presumably due to low levels of endogenous estrogens. However, exposure of males to exogenous estrogenic mimics can result elevated. Therefore, the VTG in fish can be used as a useful biomarker for appropriate tools of endocrine disrupting compounds effects. In this studies, we prepared the test methods that can measure the plasma VTG level in the gobies that live in polluted area with mimic estrogen. For the purpose, we purified VTG of floating goby(Chaenogobius annularis) and prepared specific monoclonal and polyclonal antisera to yolk protein, then developed a sandwich competitive ELISA system for measurement of plasma VTG levels. Validation for the ELISA system using monoclonal and polyclonal antibodies against VTG was tested. The absorbance curve of serial dilutions of serum from vitellogenic female was paralleled to the standard curve of VTG, but normal male was not paralleled. The developed sandwich ELISA system was measured for VTG levels in plasma of common goby(Acanthogobius flaviman) and javeline goby(A. hasta) as well as in plasma of floating goby(C. annularis).

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Distribution of Organic and Inorganic Arsenic Species in Groundwater and Surface Water Around the Ulsan Mine (울산광산 주변지역 수계에서 유기 및 무기 비소 종 분포)

  • Kim, Youn-Tae;Woo, Nam-Chil;Yoon, Hye-On;Yoon, Cheol-Ho
    • Economic and Environmental Geology
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    • v.39 no.6 s.181
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    • pp.689-697
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    • 2006
  • Distribution and speciation of arsenic in water resources was investigated in the Ulsan mine area. In 62% of uoundwater samples from the mine area, total As concentrations exceeded 0.05 mg/l, the Korean Drinking Water Standard. As(V) was the major type in groundwater with minor As(III). Arsenic species appeared to be in transition stages following redox changes after exposure to the air through the monitoring wells. In areas around the mine, the mine and Cheongog spring appeared to be the sources of arsenic contamination of water resources. The spring showed 0.345 mg/1-As, as much as seven times of the Korean standard. Groundwater and stream samples showed As-concentrations greater than 0.05 mg/l in 30% and 33% samples, respectively, and 60 and 67% of samples exceeded 0.01 mg/l of WHO guideline, respectively. Again, As(V) was a dominant species, however, several samples had As(III) in appreciable levels. In one stream sample, organic species including DMA and AsB were detected in low levels, probably resulted from transformation or related biogeochemical processes.

A Study on the Habitat Environment Survey and Conservation of Rhododendron micranthum in National Baekdudaegan Arboretum (국립백두대간수목원 꼬리진달래 자생지 환경특성 및 보전방안)

  • Chung, Bo-Kwang;Gang, Sin-Gu;Bae, Jun-Gyu;Kim, Jae-Hyeon;Lee, Juyoung;Chang, Jeong-Won;Lee, Sang-Hyun;Lee, Yeong-Su;An, Min-Woo;Kim, Gi-Song
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.19 no.6
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    • pp.87-100
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    • 2016
  • This research was performed for the purpose of providing useful and base data and preservation strategy of growth characteristics of Rhododendron micranthum. From the result of research, wild growth area of Rhododendron micranthum was discovered near steep forest road on 695~901m in altitude above sea level of Baekdu-daegan National Arboretum. Vegetation colony structure of Site I was resulted in Pinus densiflora(tree stratum M.I.P.=45.3%) is dominants, Betula schmidtii(arborescent M.I.P.=9.8%) is second dominant, and Site III resulted in Pinus densiflora(tree stratum M.I.P.=30.5%) is dominants, Betula schmidtii(arborescent M.I.P.=10.3%) is second dominant. Site II resulted in Betula schmidtii(arborescent M.I.P.=30.4%) is dominants and Pinus densiflora tree(tree stratum M.I.P.=8.3%) is second dominants. For herbs, it was researched that the rate of one year herb of chrysanthemum family(10), rice family(3), sedge(2) was higher than near forest area. Among Species diversity index, that of Shannon was in range of 0.7348~1.1090. After comparing this with similar area, this value was relatively low. Dominance analysis result was analyzed that various groups are evenly distributed. Monthly highest average temperature is $23.9^{\circ}$ in Site A and Site B, and monthly lowest average temperature is $-4.5^{\circ}C$ in Site I and $-4.7^{\circ}C$ in Site II. Monthly maximum average humidity is 85.6% in Site I and 83.2% in Site II. Monthly minimum average humidity is 60.9% in Site I and 60.8% in Site II and it showed that these 2 area are located far away but have similar temperature and humidity characteristic and climate environment is similar. It was analyzed that humidity is high with the result of annual average humidity is over 60%. In growth characteristic, it showed that doubt damage of Stephanitis pyrioides, exposure of roots, peeled bark was discovered and supplement on this is urgent.

FMEA of Electrostatic Precipitator for Preventive Maintenance (전기집진기 예지보전 단계에서의 고장모드영향분석)

  • Han, Seung-Hun;Lee, Jeong-Uk;Lee, Sun-Youp;Hwang, Jong-Deok;Kang, Dae-Kon
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.26 no.6
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    • pp.706-714
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    • 2020
  • Currently, 90 % of the world's population breathes air with a fine dust content exceeding the World Health Organization's annual average exposure limit (10 ㎍/㎥). Global efforts have been devoted toward reducing secondary pollutants and ultra-fine dust through regulations on nitrogen oxides released over land and sea. Domestic efforts have also aimed at creating clean marine environments by reducing sulfur emissions, which are the primary cause of dust accumulation in ships, through developing and distributing environment-friendly ships. Among the technologies for reducing harmful emissions from diesel engines, electrostatic precipitator offer several advantages such as a low pressure loss, high dust collection efficiency, and NOx removal and maintenance. This study aims to increase the durability of a ship by improving equipment quality through failure mode effects analysis for the preventive maintenance of an electrostatic precipitator that was developed for reducing fine dust particles emitted from the 2,427 kW marine diesel engines in ships with a gross tonnage of 999 tons. With regard to risk priority, failure mode 241 (poor dust capture efficiency) was the highest, with an RPN of 180. It was necessary to determine the high-risk failure mode in the collecting electrode and manage it intensively. This was caused by clearance defects, owing to vibrations and consequent pin loosening. Given that pin loosening is mainly caused by vibrations generated in the hull or equipment, it is necessary to manage the position of pin loosening.

Empirical Analysis on the Disparity between Willingness to Pay and Willingness to Accept for Drinking Water Risks : Using Experimental Market Method (비시장재에 대한 WTP와 WTA 격차에 대한 실증분석 : 실험시장접근법을 이용한 음용수 건강위험을 사례로)

  • Eom, Young Sook
    • Environmental and Resource Economics Review
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    • v.17 no.3
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    • pp.135-166
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    • 2008
  • This paper reports the empirical results of comparing the willingness to pay(WTP) for health risk reductions and the willingness to accept(WTA) for risk increases using experimental market methods in the first time in Korea. Health risks were defined as probabilities of premature death from exposure to one of As, Pb, and THM in tap water. A total of six experimental markets with 15 participants in each experiments were held using 20 repetitive Vickrey second-price sealed-bid auctions. To compare the effects of market experiences, trading a marketed good, candy bar, was introduced before the trading the non-marketed good, drinking water risks. Moreover, an objective risk information was provided after the first 10 trials to incorporate learning processes. Regardless of marketed or non-marketed goods, the mean of WTA exceeded the mean of WTP at the first auction trial. As experimental trials proceeded, the disparity between WTA and WTP for marketed goods disappeared. However results for non-marketed goods were rather mixed to the extent that WTA for health risks from As (relatively high risk leves) were significantly larger than WTP, while there were no significant difference between WTA and WTP for health risks fro Pb and THM (relatively low risk levels). On the other hand, participants seemed to respond in a 'rational' manner to the objective risk information provided, with positive learning effects of market-like experience(especially in the WTA experiments).

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Differentiated Thyroid Carcinoma Risk Factors in French Polynesia

  • Xhaard, Constance;Ren, Yan;Clero, Enora;Maillard, Stephane;Brindel, Pauline;Rachedi, Frederique;Boissin, Jean-Louis;Sebbag, Joseph;Shan, Larrys;Bost-Bezeaud, Frederique;Petitdidier, Patrick;Drozdovitch, Vladimir;Doyon, Francoise;Rubino, Carole;de Vathaire, Florent
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.6
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    • pp.2675-2680
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    • 2014
  • Background: To investigate differentiated thyroid cancer risk factors in natives of French Polynesia is of interest because of the very high incidence of this cancer in the archipelago. Materials and Methods: To assess the role of various potential risk factors of thyroid cancer in the natives of French Polynesia we performed a case-control study. The study included almost all the French Polynesians diagnosed with differentiated thyroid carcinoma between 1981 and 2003 (n=229) and 373 French Polynesian control individuals from the general population without cancer. Results: Thyroid radiation dose received from nuclear fallout before the age of 15, a personal history of neck or/and head medical irradiation, obesity, tallness, large number of children, an artificial menopause, a familial history of thyroid cancer, a low dietary iodine intake, and having a spring as the main source of drinking water were found to be significant risk factors. No roles of smoking habits, alcohol consumption, iodine containing drugs, and exposure to pesticides were evidenced. Conclusions: Except for smoking, differentiated thyroid carcinoma risk factors in natives of French Polynesia are similar to those in other populations. Our finding on the role of having a spring as a drinking water origin is coherent with some other studies and could be due to geological factors.

Effects of Hypobaric Conditions on Apoptosis Signalling Pathways in HeLa Cells

  • Arican, Gul Ozcan;Khalilia, Walid;Serbes, Ugur;Akman, Gizem;Cetin, Idil;Arican, Ercan
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.12
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    • pp.5043-5047
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    • 2014
  • Nowadays increasing effectiveness in cancer therapy and investigation of formation of new strategies that enhance antiproliferative activity against target organs has become a subject of interest. Although the molecular mechanisms of apoptosis can not be fully explained, it is known that cell suicide program existing in their memory genetically is activated by pathophysiological conditions and events such as oxidative stress. Low pressure (hypobaric) conditions that create hypoxia promote apoptosis by inhibiting cell cycling. In this study, determination of the effects of fractional hypobaric applications at different times on HeLa cells at cellular and molecular levels were targeted. Experiments were carried out under hypobaric conditions (35.2 kPa) in a specially designed hypobaric cabin including 2% $O_2$ and 98% N. Application of fractional hypobaric conditions was repeated two times for 3 hours with an interval of 24 hours. At the end of the implementation period cells were allowed to incubate for 24 hours for activation of repair mechanisms. Cell kinetic parameters such as growth rate (MTT) and apoptotic index were used in determination of the effect of hypobaric conditions on HeLa cells. Also in our study expression levels of the Bcl-2 gene family that have regulatory roles in apoptosis were determined by the RT-PCR technique to evaluate molecular mechanisms. The results showed that antiproliferative effect of hypobaric conditions on HeLa cells started three hours from the time of application and increased depending on the period of exposure. While there was a significant decrease in growth rate values, there was a significant increase in apoptotic index values (p<0.01). Also molecular studies showed that hypobaric conditions caused a significant increase in expression level of proapoptotic gene Bax and significant decrease in antiapoptotic Bfl-1. Consequently fractional application of hypobaric conditions on HeLa cell cultures increased both antiproliferative and apoptotic effects and these effects were triggered by the Bax gene.

Degradation and Stability of Organic-Inorganic Perovskite Solar Cells (유 무기 페로브스카이트 태양전지의 열화와 안정성)

  • Cho, Kyungjin;Kim, Seongtak;Bae, Soohyun;Chung, Taewon;Lee, Sang-won;Lee, Kyung Dong;Lee, Seunghun;Kwon, Guhan;Ahn, Seh-Won;Lee, Heon-Min;Ko, Min Jae;Kang, Yoonmook;Lee, Hae-seok;Kim, Donghwan
    • Current Photovoltaic Research
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    • v.4 no.2
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    • pp.68-79
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    • 2016
  • The power conversion efficiency of perovskite solar cells has remarkably increased from 3.81% to 22.1% in the past 6 years. Perovskite solar cells, which are based on the perovskite crystal structure, are fabricated using organic-inorganic hybrid materials. The advantages of these solar cells are their low cost and simple fabrication procedure. Also, they have a band gap of about 1.6 eV and effectively absorb light in the visible region. For the commercialization of perovskite solar cells in the field of photovoltaics, the issue of their long term stability cannot be overlooked. Although the development of perovskite solar cells is unprecedented, their main drawback is the degradation of the perovskite structure by moisture. This degradation is accelerated by exposure to UV light, temperature, and external bias. This paper reviews the aforesaid reasons for perovskite solar cell degradation. We also discuss the research directions that can lead to the development of perovskite solar cells with high stability.

Study on Oxidation Properties of Biodiesel Blended Fuels according to Storage Circumstances (저장 환경에 따른 바이오디젤 혼합연료의 산화특성 규명 연구)

  • Min, Kyong-Il;Yim, Eui-Soon;Jung, Chung-Sub;Kim, Jae-Kon;Na, Byung-Ki
    • Journal of the Korean Applied Science and Technology
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    • v.30 no.4
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    • pp.701-714
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    • 2013
  • Recently, due to the activation policy of biodiesel, the blending biodiesel to petroleum product is increasing. Low-temperature fluidity and oxidation stability are the most significant issues to the marketers and end users of biodiesel. Thus, the way to control the quality of biodiesel blended fuels was investigated by duplicating the real storage situation of biodiesel blended fuels and evaluating the effect of oxidation trend and quality degradation. From the results of oxidation degradation test of biodiesel blended fuels, no special quality degradation has been observed through the evaporation for 18 weeks in a summer season under a storage circumstance without exposure to the sun light. However, the severe quality degradation was observed in PE vessel in only 2 weeks of storage. This oxidation degradation was also verified with FT-IR spectrum change. However, the special symptom cannot be distinguished by the quality test because the quality specifications were satisfied despite of the drastic oxidation degradation. Namely, the problem in a vehicle could be occurred by oxidation materials(polymer, organic acid. etc.) under oxidation even though it satisfied the quality specification.

Summer Algal Communities in the Rocky Shore of South Sea of Korea -II. Subtidal communities- (남해의 하계 해조군집 -II. 조하대의 군집-)

  • KANG Rae-Seon;JE Jong-Geel;SOHN Chul-Hyun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.26 no.2
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    • pp.182-197
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    • 1993
  • Algal community on the subtidal rocky zone of the South Sea of Korea was divided into two or three sequencing zones. The upper subtidal zone was characterized by the wave exposure-tolerant surf wrack(Pachymeniopsis, Gigartina), which formed dense swirling carpet. Its vertical range was from the surface to $3{\sim}5$ meters in depth, and more deeply extended in turbid waters including Sorido, Yokchido, Pijindo, Manjedo. The mid subtidal zone ranging from 5 to 25 meters in depth was characterized by a large brown algal forest (Ecklenia, Sargassum). But it was generally unrecognizable in that turbid waters, in which the vertical limit of vegetation was at most $10{\sim}15$ meters in depth. The low subtidal zone was characterized by a general lack of algal species and was not easily distinguished from the mid or sometimes from the upper zone. There was a distinct difference in abundance of vegetation between turbid waters and clear waters including Munsom, Kwantaldo, Yosodo, Hongdo, Ch'ujado. In turbid waters the vegetation was much poorer because the tubidity caused from the muddy sediment inhibited an algae to settle down and to grow up. On the basis of the phytogeographical methods using UPGMA, the 10 studied islands were classified into two groups, Munsom and the others. This floristic discontinuity between the two groups might be caused from the difference of water temperature.

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