• Title/Summary/Keyword: Low Temperature preservation

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Effects of low temperature-adapted Saccharomyces cerevisiae Y297 strain and fermentation temperature on the quality characteristics of Yakju (저온 적응성 효모와 발효온도에 따른 약주의 품질특성 변화)

  • Seo, Dong-Jun;Yeo, Soo-Hwan;Mun, Ji-Young;Baek, Seong Yeol
    • Food Science and Preservation
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    • v.23 no.5
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    • pp.666-672
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    • 2016
  • The objective of this study was to evaluate the effects of low temperature-adapted Saccharomyces cerevisiae Y297 and fermentation temperatures on the quality of Yakju brewed. Physicochemical properties of Yakju brewed were compared pH, total acidity, ethanol, free amino acid, organic acid contents, and volatile flavor compounds in S. cerevisiae Y297 with control treatment. Cooked non-glutinous rice and saccharogenic amylase in koji were mixed with ethanol-producing yeasts and then fermented at $15^{\circ}C$, $20^{\circ}C$, and $25^{\circ}C$ for 20 days. Yakju brewed using the Y297 treatment showed the highest ethanol yield (17.9%) at $20^{\circ}C$. Expression of heat shock protein (HSP) 104 was evaluated by immunoblotting as an indication of adaptation to low temperatures ($15^{\circ}C$); levels of the HSP104 protein were higher in the Y297 treatment than in the control. Organic acid analysis showed that the lactic acid content of Yakju brewed using the control was the highest at $25^{\circ}C$. Finally, free amino acid analysis showed that the Y297 treatment had a higher proportion of essential amino acids than the control. Overall, these results indicate that S. cerevisiae Y297 could be used as a suitable yeast for Yakju brewed under low temperature ($15^{\circ}C$) condition.

Studies on the Prevention of Greening in Crushed Garlic from Bulbs Stored in Low Temperature (마늘의 저온저장후 파쇄마늘 제조시 발생하는 녹변방지 연구)

  • Choi, Sun-Tay;Lim, Byung-Seon;Mok, Il-Gin;Lee, Chong-Suk;Chang, Kyu-Seob
    • Food Science and Preservation
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    • v.7 no.3
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    • pp.245-248
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    • 2000
  • It was attempted to find a method to prevent greening of crushed garlic. The storage conditions and heat treatments before crushing were tested for the prevention of greening in crushed garlic. 'Namhae' garlic (sub-tropical type), 'Seosan' and 'Danyang' garlic (traditional) type for cool area) were stored in room temperature(20${\pm}5^{\circ}C$, low temperature(0$^{\circ}C$), and CA(O$_2\;3%,\;CO_2$ 5%) storage for five months, and their samples were crushed. The crushed garlic had no significant differences in greening according to the cultivars. Greening did not occur for the crushed garlic from bulbs stored either in room temperature or CA storage. Greening was the unique symptom observed in crushed garlic from bulbs stored in low temperature. For the prevention of greening, heat treatment was conducted at 40, 35 and 30$^{\circ}C$ prior to make the crushed garlic from bulbs which were stored in low temperature. The effective duration of heat treatment before crushing was 7 days at 30$^{\circ}C$, 3 days at 35$^{\circ}C$, and 1 days at 40$^{\circ}C$ Greening was most effectively prevented in 1 day at 40$^{\circ}C$ treatment. During the heat treatments, changes in enzymatic pyruvic acid content and sprouting rate were slightly observed in garlic bulbs treated at 40$^{\circ}C$ for 1 day.

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Changes of Characteristics in Red Pepper by Various Freezing and Thawing Methods (홍고추의 저장온도 및 해동조건에 따른 물리화학적 특성 변화)

  • Lee, Hye-Eun;Lim, Chai-Il;Do, Kyung-Ran
    • Food Science and Preservation
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    • v.14 no.3
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    • pp.227-232
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    • 2007
  • The development of an effective long-term storage protocol for harvested fresh pepper is urgently required to increase the market for pepper products. The protocol must minimize quality loss, so that the product may be used either as a spice or as a raw material for processed pepper products, both in the home and in food processing plants. We investigated the optimum size of pepper fruits, freezing temperatures, storage periods, and thawing methods, to establish an optimum storage protocol. This study was conducted not only to develop freezing and thawing methods for long term storage of harvested red pepper, but also to develop processed pepper products utilizing the stored pepper. We aimed to expand the pepper products market and to increase the incomes of pepper growers. Whole red pepper, sliced red pepper, and crushed red pepper were frozen and stored at $-5^{\circ}C,\;-20^{\circ}C,\;or\;-40^{\circ}C$. The soluble solid content and the vitamin C level showed maximal stability at $-40^{\circ}C$, although total free sugars decreased on storage at all temperatures tested. Such Changes were more marked at $-5^{\circ}C$ than at the other(lower) temperature tested. The vitamin C content of whole red pepper was higher than that of sliced red pepper or crushed red pepper. Room-temperature thawing resulted in twice the drip loss seen on low temperature($5^{\circ}C$) thawing or microwave oven thawing. Brown discoloration was a serious problem with room temperature thawing. Total free sugars were higher in samples thawed at low temperature or in the microwave oven, compared to the level seen after room-temperature thawing. pepper samples thawed at low temperature scored higher in sensory tests than samples thawed at room temperature.

Production of spherical granule type yogurt with improving convenience and preservation (편리성 및 저장성이 증진된 구형과립 요구르트 제조)

  • Shin, Myung-Gon
    • Food Science and Preservation
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    • v.24 no.5
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    • pp.559-564
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    • 2017
  • Yogurt contains many microorganisms that are beneficial to human health, and is a probiotic that supplies many nutrients such as calcium and protein. It is difficult to safety preserve for a long time because it possesses a high content of water. To address this problem, powdered "instant" yogurt has been developed, but it has flaws low flowability and solubility. Therefore, yogurt was granulated using a fluidized bed granulator to increase flowability and solubility. The fluidized bed granulator was designed by using response surface methodology (RSM), whose variables were feeding rate (FR), atomization air pressure (AP) and product temperature (PT). After being granulated, the yogurt was analyzed for yield and lactic acid bacteria count. The maximum yield of yogurt granules was 79.42%, at FR of 0.54 mL/min, AP of 2.64 kPa, and PT of $58.18^{\circ}C$, and the colony count for lactic acid bacteria was more than $6log^{10}\;CFU/g$. Therefore, spherical granulation of yogurt using a fluidized bed granulator could be used for making convenient probiotic products with improved flowability and solubility.

The investigation of conservation methods and destruction regions of papers under the various temperature and humidity changes (온.습도 변화에 따른 양지의 손상원인 및 보존방안 연구)

  • Lee, Hye-Yun;Chung, Yong-Jae;Lee, Kyu-Sik;Han, Sung-Hee
    • 보존과학연구
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    • s.21
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    • pp.145-175
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    • 2000
  • To examine the method for preservation of papers, 9 kinds of papers were purchased and pretreated for 6 months with various artificial conditions which were controlled as 3 temperature classes($20^{\circ}C$, $80^{\circ}C$, $-20^{\circ}C$&$20^{\circ}C$) and 4 humidity classes(40%, 60%, 80%, 100%).Whiteness, acidity and folding endurance were measured to examine the state of papers pretreated under each condition. From the resultexamining the state, all the papers were best condition at low temperature($20^{\circ}C$) and low humidity(40%) and damaged at high temperature($80^{\circ}C$) and high humidity(100%).The values of whiteness, acidity, and folding endurance were measure din relation with deterioration of papers. According to damage of papers caused by the chemical agent, papers became to be acidic, increased color difference and reduced the force of folding endurance. The effect of change of temperature($-20^{\circ}C$&$20^{\circ}C$) on degradation of papers was not well acquired. Because the period of investigation was not long enough to acquire the data. So the more studies are needed to find the cause of damage of papers.

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Development of the Corrugated fiberboard Box for Cold-chain Distribution of Chinese Cabbage (배추의 저온유통용 골판지포장상자 개발)

  • Lee, Won-Og;Yun, Hong-Sun;Jeong, Hoon;Lee, Hyun-dong;Cho, Kwang-Hwan;Kim, Man-Soo
    • Food Science and Preservation
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    • v.10 no.1
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    • pp.23-27
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    • 2003
  • This study was conducted to develop the corrugated fiberboard box which is suitable to pre-cooling and low temperature distribution of chinese cabbage Consider from a cooling performance viewpoint, the folding type box with 5.4% vent hole ratio was most efficient for fast cooling and even temperature distribution in the packaging box. At the end of storage periods, the compressive strength of the folding type box was less than the bliss type box. However, the compressive strength of the folding type box after storage was higher than required safety compressive strength for long term storage. So the folding type box was considered to have no problems toy practical use. The shelf-life of the chinese cabbage packaged with the developed box was 6∼7 days which was 2∼3 days longer than usual packaging.

Effects of Preservation Period at Low Temperature on the Mycelial Growth and the Lignocellulolytic Enzyme Activities of Basidiomycetes (저온보존기간이 담자균류의 균사생장과 목질분해효소의 활성에 미치는 영향)

  • Jeong, Yeun Sug;Ka, Kang-Hyeon
    • The Korean Journal of Mycology
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    • v.42 no.4
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    • pp.322-327
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    • 2014
  • Subculture is the most common method for preservation fungi, but has a disadvantage of accumulation of spontaneous mutations during the repeated subculture. To reduce the subculture frequency, the effect of preservation period at $4^{\circ}C$ in a slant culture was examined on the mycelia growth and lignocellulolytic enzyme activities of various basidiomycetes. Mushrooms, including Stereum ostrea, Coprinellus micaeus, Trametes versicolor, Hypholoma fasciculare, Wolfiporia extensa, Pleurotus pulmonarius, Piptoporus betulinus and Ganoderma applanatum were not affected by the preservation period more than two years, indicating that they can be maintained by subculture every two years. Some other tested fungal strains showed a significant decrease in both viability and enzyme activity when they were maintained for two years, suggesting that they should be subcultured at least once in a year. A little correlation was found between the recovery of mycelial growth and extracellular enzyme activity. In conclusion, mycelial activity and enzyme activity according to storage period is expected to be a way of deciding on subculture times for fungal preservation.

Changes of Physicochemical Characteristics of Acanthopanax senticosus Extract during Storage (오가피 추출액의 저장조건에 따른 이화학적 특성변화)

  • Chung Hun-Sik;Youn Kwang-Sup
    • Food Science and Preservation
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    • v.12 no.3
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    • pp.204-208
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    • 2005
  • This study was conducted to investigate the effects of temperature and packaging on the quality characteristics of hot-water extract from Acanthopanax senticosus. The herb samples, ground and sifted($8{\sim}20$ mesh), were extracted by a boiling($100^{\circ}C$) water under the atmospheric pressure for 2 hrs. The extract was packed with out nylon PE(0.08 mm) pouch and kept under the condition(40 and $60^{\circ}C$) for 10 days. The reduction of turbidity, pH, polyphenol amount and DPPH radical scavenging activity of extract were retarded by packaging under the low temperature. Titratable acidity was increased with packaging at high temperature. Soluble solid content was not affected by packaging and temperature condition. The changes of quality characteristics followed the first-order reaction rate. Arrhenius equation was applied to determine the effects of temperature on the reaction rate constant, showing high $R^2$. These results suggested that packaging under lower temperature can be utilized for maintaining the quality of A senticosus extract.

Physicochemical Properties of Satsuma Mandarin according to Harvest Year (수확년도에 따른 조생온주 밀감의 품질변화)

  • 고정삼;양영택
    • Food Science and Preservation
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    • v.5 no.1
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    • pp.1-6
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    • 1998
  • Physicochemical properties of early cultivar of Satsuma mandarin(Citrus unshiu Marc. var. miyagawa) according to harvesting year during 1990 to 1995 were investigated. Climatic conditions of every year affected the quality of citrus fruits. Compared to other harvesting years, flesh ratio and soluble solids were higher, but acid content was lower on citrus fruits produced in 1994, as for good climatic conditions during growth of fruits. Especially, acid content of fruits produced in 1993 was higher, compared to those of years, as for low mean temperature and high rainfalls. Fruit weight, peel thickness and soluble solids had a good linear correlation to fruits size, but acid content and flesh ratio had not correlation derived from difference of individual fruits. From the climatic data, the prediction of fruit quality would be possible in some degree before harvest. It seemed recommend to ably the flexibe method by harvesting year for the quality stanardization of citrus fruit.

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Effect of Film Packaging on Storage Life of Grape, Sheridan (Polyethylene Film포장이 포도 Sheridan의 저장력에 미치는 영향)

  • 남상영;김경미
    • Food Science and Preservation
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    • v.4 no.1
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    • pp.11-15
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    • 1997
  • This experiment carried out to find the storage life according to the kinds of packaging material. Sheridan(Grape) which was fumigated SO2 were wrapped with polyethylenes(Bio-PE and PE) and stored at $0^{\circ}C$ under 90% RH modified condition. The resorts were summarized as follow. Natural weight loss was increased according to the storage time during the 135days storage that was only 1.0-1.7% in sealing section while 10% in non-sealing section. Abonrmal fruits were increased as the storage time was increased and it was 6.6-6.7% in sealing section while 100% in non-sealing during the 135days storage. Quality of appearance and taste are better in sealing section than non-sealing section and it was good in Bio-PE sealing section between packaging materials. Moistrue content was de creased as the storage range was increased and the decreasing rate of that during the storage was 4.9-5.2% in sealing section between treatments. During the storage range increased, the soluble solid degree was increased in non-sealing section but decreased in sealing section.

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