• 제목/요약/키워드: Low Fin Surface

검색결과 47건 처리시간 0.024초

Developing a Virus-Binding Bacterium Expressing Mx Protein on the Bacterial Surface to Prevent Grouper Nervous Necrosis Virus Infection

  • Lin, Chia-Hua;Chen, Jun-Jie;Cheng, Chiu-Min
    • Journal of Microbiology and Biotechnology
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    • 제31권8호
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    • pp.1088-1097
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    • 2021
  • Grouper nervous necrosis virus (GNNV) infection causes mass grouper mortality, leading to substantial economic loss in Taiwan. Traditional methods of controlling GNNV infections involve the challenge of controlling disinfectant doses; low doses are ineffective, whereas high doses may cause environmental damage. Identifying potential methods to safely control GNNV infection to prevent viral outbreaks is essential. We engineered a virus-binding bacterium expressing a myxovirus resistance (Mx) protein on its surface for GNNV removal from phosphate-buffered saline (PBS), thus increasing the survival of grouper fin (GF-1) cells. We fused the grouper Mx protein (which recognizes and binds to the coat protein of GNNV) to the C-terminus of outer membrane lipoprotein A (lpp-Mx) and to the N-terminus of a bacterial autotransporter adhesin (Mx-AIDA); these constructs were expressed on the surfaces of Escherichia coli BL21 (BL21/lpp-Mx and BL21/Mx-AIDA). We examined bacterial surface expression capacity and GNNV binding activity through enzyme-linked immunosorbent assay; we also evaluated the GNNV removal efficacy of the bacteria and viral cytotoxicity after bacterial adsorption treatment. Although both constructs were successfully expressed, only BL21/lpp-Mx exhibited GNNV binding activity; BL21/lpp-Mx cells removed GNNV and protected GF-1 cells from GNNV infection more efficiently. Moreover, salinity affected the GNNV removal efficacy of BL21/lpp-Mx. Thus, our GNNV-binding bacterium is an efficient microparticle for removing GNNV from 10‰ brackish water and for preventing GNNV infection in groupers.

착상을 고려한 극저온 질소-대기 열교환기의 해석 (Analysis of a Cryogenic Nitrogen-Ambient Air Heat Exchanger Including Frost Formation)

  • 최권일;장호명
    • 설비공학논문집
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    • 제12권9호
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    • pp.825-834
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    • 2000
  • A heat exchanger analysis is performed to investigate the heating characteristics of cryogenic nitrogen by ambient air for the purpose of cryogenic automotive propulsion. The heat exchanger is a concentric triple-passage for supercritical nitrogen, and the radial fins are attached on the outermost tube for the crossflow of ambient air. The temperature distribution is calculated for the nitrogen along the passage, including the real gas properties of nitrogen, the fluid convections and the conductions through the tube walls and the fins. Since the wall temperature of the outer (ambient side) tube is very low in most cases, a heavy frost can be formed on the surface, affecting the heat exchange performance. By the method of the similarity between the heat and the mass transfer of moist air, the frost growth and the time-dependent effectiveness of the heat exchanger are calculated for various operating conditions. It is concluded that the frost formation can augment the heating of nitrogen during the initial period because of the latent heat, then gradually degrades the heat exchange because of the increased thermal resistance. Practical design issues are discussed for the flow rate of nitrogen, the velocity and humidity of ambient air, and the sizes of the fin.

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Dynamics of Hexavalent Chromium in Four Types of Aquaculture Ponds and Its Effects on the Morphology and Behavior of Cultured Clarias gariepinus (Burchell 1822)

  • Mustapha, Moshood Keke
    • Toxicological Research
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    • 제33권2호
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    • pp.119-124
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    • 2017
  • Hexavalent chromium is a bio accumulative toxic metal in water and fish. It enters aquaculture ponds mainly through anthropogenic sources. Hexavalent chromium concentrations and its effects on the morphology and behavior of Clarias gariepinus were investigated from four aquaculture ponds for 12 weeks. Chromium was measured using diphenyl carbohdrazide method; alkalinity and hardness were measured using colometric method and analyzed with Bench Photometer. Temperature and pH were measured using pH/EC/TDS/Temp combined tester. Temporal and spatial replications of samples were done with triplicates morphological and behavioural effects of the metal on fish were observed visually. Chromium ranged from no detection to 0.05 mg/L, alkalinity 105 to 245 mg/L, hardness 80 to 165 mg/L, pH 6.35 to 8.03 and temperature 29.1 to $35.9^{\circ}C$. Trend in the chromium concentrations in the ponds is natural > earthen > concrete > collapsible. There was a significant difference (P < 0.05) in chromium, alkalinity, water hardness, pH and temperature among the four ponds. Significant positive correlation also existed between alkalinity, water hardness, pH, with chromium. Morphological and behavioural changes observed in the fish include irregular swimming, frequent coming to the surface, dark body colouration, mucous secretion on the body, erosion of gill epithelium, fin disintegration, abdominal distension and lethargy. High chromium concentration in natural pond was due to anthropogenic run-off of materials in to the pond. Acidic pH, low alkalinity, low water hardness also contributed to the high chromium concentration. Morphological and behavioural changes observed were attributed to the high concentrations, toxicity and bio accumulative effect of the metal. Toxicity of chromium to fish in aquaculture could threaten food security. Watershed best management practices and remediation could be adopted to reduce the effects of toxicity of chromium on pond water quality, fish flesh quality and fish welfare.

디젤 차량의 보조 난방을 위한 PTC 히터 개발 (Development of a PTC Heater for Supplementary Heating in a Diesel Vehicle)

  • 신윤혁;김성철
    • 한국산학기술학회논문지
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    • 제15권2호
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    • pp.666-671
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    • 2014
  • 최근 디젤 차량과 같은 내연기관의 고효율화에 따라, 보조난방 열원으로서 PTC 히터의 사용이 증가하는 추세이다. 디젤 차량의 시동 초기에는 냉각수의 온도가 난방으로 직접 사용하기에 충분히 높지 않으므로, 동절기 난방을 위해 보조난방 열원은 필수적이다. 본 연구에서는 스크린 인쇄 전극공정을 바탕으로 한 PTC 소자를 제작하였고, 이를 활용한 PTC 소자 모듈 및 히터를 설계 및 제작하였다. PTC 소자 모듈의 방열핀 접촉형상 및 전열소자의 크기 변경에 따른 난방성능 변화를 열유동 해석을 통해 분석하였고, 난방성능 실험을 수행하여 PTC 히터의 난방성능 및 효율 특성을 살펴보았다. PTC 히터 시작품의 경우, 기존 PTC 히터와 동등한 수준 이상의 난방성능 및 효율을 나타내었으며, 향후 이를 바탕으로 PTC 소자와 히터에 대한 공정개선 및 성능증대 연구를 수행할 계획이다.

중고도에서 운용되는 측 추력 제어 요격체에 대한 제트 간섭 유동 분석 (Jet Interaction Flow Analysis of Lateral Jet Controlled Interceptor Operating at Medium Altitude)

  • 최경준;이성욱;오광석;김종암
    • 한국항공우주학회지
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    • 제46권12호
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    • pp.986-993
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    • 2018
  • 측 추력 제트는 유도무기의 자세제어 및 궤도 천이 기동을 하는 데 있어 기존의 핀과 같이 제어 면을 이용한 방식보다 우수한 기동성을 갖는다. 하지만 초음속 영역에서 비행 시 측 추력 제트로 인한 제트 간섭 유동이 발생하며 충격파와 경계층 유동, 와류 유동의 상호 작용으로 인해 매우 복잡한 유동 구조를 나타낸다. 특히 직격 파괴(hit-to-kill) 방식의 요격체의 경우 정밀한 제어 및 기동이 요구되기 때문에 제트 간섭 유동이 미치는 영향에 대한 분석이 필요하다. 기존의 제트 간섭 해석은 저고도 운용 조건에서 주로 수행되었으나 중고도 운용 조건의 경우 해석 사례가 많지 않으며 대기 조건으로 인해 분사 제트 유동이 상대적으로 크게 발달하는 특징을 갖는다. 본 연구에서는 중고도에서 비행하는 요격체 형상에 대해 받음각 조건에 따라 제트 간섭 유동 해석을 수행하였다. 해석 결과를 바탕으로 유동장의 구조적인 변화 특성을 분석하였으며, 공력 계수의 변화를 비교하였다.

자동차 전면 글래스용 FM 대역 투명 모노폴 안테나 (Transparent Monopole Antenna on the Front Glass of an Automobile for FM Band)

  • 이주형;정창원
    • 한국전자파학회논문지
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    • 제29권7호
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    • pp.477-483
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    • 2018
  • 본 논문에서는 FM 방송대역(88~108 MHz)에서 동작하는 자동차 전면 글래스 상에 설계되는 투명 안테나를 제안한다. 투명 안테나는 전형적으로 차량 지붕에 설치된 샤크핀 안테나의 공간적 제한을 피하기 위해 차량 전면 글래스에 구현하였다. 전면 글래스에 설계되는 안테나는 낮은 수심감도와 다른 서비스대역의 안테나로부터의 방사간섭 문제를 합리적으로 해결할 수 있다. 전면 글래스는 고유한 closed-line 구조를 가지고 있으며, 이 구조로 인해 전면 글래스 외곽의 차체 표면으로 전류가 흐르게 되어 FM 방송대역 이전 부분에서 첫 공진점이 발생한다. Closed-line 구조를 이용한 표면 전류 길이 제어를 통해 첫 공진점이 FM 대역에서 동작하는 안테나를 설계하였다. 또한, RF 수동소자로 설계하기 적합한 투명전극 소자 마이크로 메탈메쉬 필름의 투명성을 통해 시각적 인식의 최소화 하였다. 측정된 안테나는 FM 대역에서 반사계수는 -6 dB 이하이며, average peak gain은 -0.9 dB이다. 본 연구를 통해 제안된 전면 글래스 투명안테나는 미래의 자동차 안테나 개발에 필요한 공간과 설계 자유에 대한 가능성을 보여주었다.

설비공학 분야의 최근 연구 동향: 2011년 학회지 논문에 대한 종합적 고찰 (Recent Progress in Air-Conditioning and Refrigeration Research: A Review of Papers Published in the Korean Journal of Air-Conditioning and Refrigeration Engineering in 2011)

  • 한화택;이대영;김서영;최종민;백용규;김수민
    • 설비공학논문집
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    • 제24권6호
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    • pp.521-537
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    • 2012
  • This article reviews the papers published in the Korean Journal of Air-Conditioning and Refrigeration Engineering during 2011. It is intended to understand the status of current research in the areas of heating, cooling, ventilation, sanitation, and indoor environments of buildings and plant facilities. Conclusions are as follows. (1) Research trends of thermal and fluid engineering have been surveyed as groups of fluid machinery and fluid flow, thermodynamic cycle, and new and renewable energy. Various topics were presented in the field of fluid machinery and fluid flow. Research issues mainly focused on the rankine cycle in the field of thermodynamic cycle. In the new and renewable energy area, researches were presented on geothermal energy, fuel cell, biogas, reformer, solar water heating system, and metane hydration. (2) Research works on heat transfer area have been reviewed in the categories of heat transfer characteristics, pool boiling and condensing heat transfer, nanofluids and industrial heat exchangers. Researches on heat transfer characteristics included heat transfer above liquid helium surface in a cryostat, methane hydrate formation, heat and mass transfer in a liquid desiccant dehumidifier, thermoelectric air-cooling system, heat transfer in multiple slot impinging jet, and heat transfer enhancement by protrusion-in-dimples. In the area of pool boiling and condensing heat transfer, researches on pool boiling of water in low-fin and turbo-B surfaces, pool boiling of R245a, convective boiling two-phase flow in trapezoidal microchannels, condensing of FC-72 on pin-finned surfaces, and natural circulation vertical evaporator were actively performed. In the area of nanofluids, thermal characteristics of heat pipes using water-based MWCNT nanofluids and the thermal conductivity and viscosity were measured. In the area of industrial heat exchangers, researches on fin-tube heat exchangers for waste gas heat recovery and Chevron type plate heat exchanger were implemented. (3) Refrigeration systems with alternative refrigerants such as $CO_2$, hydrocarbons, and mixed refrigerants were studied. Heating performance improvement of heat pump systems were tried applying supplementary components such as a refrigerant heater or a solar collector. The effects of frost growth were studied on the operation characteristic of refrigeration systems and the energy performance of various defrost methods were evaluated. The current situation of the domestic cold storage facilities was analyzed and the future demand was predicted. (4) In building mechanical system fields, a variety of studies were conducted to achieve effective consumption of heat and maximize efficiency of heat in buildings. Various researches were performed to maximize performance of mechanical devices and optimize the operation of HVAC systems. (5) In the fields of architectural environment and energy, diverse purposes of studies were conducted such as indoor environment, building energy, and renewable energy. In particular, renewable energy and building energy-related researches have mainly been studied as reflecting the global interests. In addition, various researches have been performed for reducing cooling load in a building using spot exhaust air, natural ventilation and energy efficiency systems.