• Journal of KIISE
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• v.43 no.11
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• pp.1233-1244
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• 2016

In the IoT network environments for LLNs(Low power and Lossy networks), IPv6 Routing Protocol for Low Power and Lossy networks(RPL) has been proposed by IETF(Internet Engineering Task Force). The goal of RPL is to create a directed acyclic graph, without loops. As recommended by the IETF standard, RPL route recovery mechanisms in the event of a failure of a node should avoid loop, loop detection, DIO Poisoning. In this process, route recovery time and control message might be increased in the sub-tree because of the repeated route search. In this paper, we suggested RPL route recovery method to solve the routing overhead problem in the sub-tree during a loss of a link in the RPL routing protocol based on IoT wireless networks. The proposed method improved local repair process by utilizing a route that could not be selected as the preferred existing parents. This reduced the traffic control packet, especially in the disconnected node's sub tree. It also resulted in a quick recovery. Our simulation results showed that the proposed RPL local repair reduced the recovery time and the traffic of control packets of RPL. According to our experiment results, the proposed method improved the recovery performance of RPL.

• Journal of Institute of Control, Robotics and Systems
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• v.17 no.2
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• pp.93-101
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• 2011

For mobile robot, recognizing its current location is very important to navigate autonomously. Especially, loop closing detection that robot recognize location where it has visited before is a kernel problem to solve localization. A considerable amount of research has been conducted on loop closing detection and localization based on appearance because vision sensor has an advantage in terms of costs and various approaching methods to solve this problem. In case of scenes that consist of repeated structures like in corridors, perceptual aliasing in which, the two different locations are recognized as the same, occurs frequently. In this paper, we propose an improved method to recognize location in the scenes which have similar structures. We extracted salient regions from images using visual attention model and calculated weights using distinctive features in the salient region. It makes possible to emphasize unique features in the scene to classify similar-looking locations. In the results of corridor recognition experiments, proposed method showed improved recognition performance. It shows 78.2% in the accuracy of single floor corridor recognition and 71.5% for multi floor corridors recognition.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2015.05a
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• pp.407-409
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• 2015

In this paper, a hardware implementation of MOD(Moving Object Detection) algorithm is described, which is based GMM(Gaussian Mixture Model) and background subtraction. The EGML(Effective Gaussian Mixture Learning) is used to model and update background. Some approximations of EGML calculations are applied to reduce hardware complexity, and pipelining technique is used to improve operating speed. Gaussian parameters are adjustable according to various environment conditions to achieve better MOD performance. MOD processor is verified by using FPGA-in-the-loop verification, and it can operate with 109 MHz clock frequency on XC5VSX95T FPGA device.

• The Transactions of the Korean Institute of Power Electronics
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• v.20 no.1
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• pp.72-80
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• 2015

In distributed generation systems, a grid-connected inverter should operate with synchronization to grid voltage. Considering that synchronization requires the phase angle of grid voltage, a phase locked loop (PLL) scheme is often used. The synchronous reference frame phase locked loop (SRF-PLL) is generally known to provide reasonable performance under ideal grid voltage. However, this scheme indicates performance degradation under the harmonic distorted or unbalanced grid voltage condition. To overcome this limitation, this paper proposes a phase and harmonic detection method of grid voltage using fast Fourier transform (FFT). To reduce the calculation time of FFT algorithm, minimum sampling data is taken from the voltage measurement to determine the phase angle and the magnitude of harmonic components. An experimental test setup for a grid-connected inverter system has been constructed. By comparative simulations and experiments under various abnormal grid voltage conditions, the proposed scheme has been proven to effectively track the phase angle of the grid voltage.

• Journal of Advanced Marine Engineering and Technology
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• v.31 no.6
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• pp.791-796
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• 2007

When 2 sets of PID controllers are coupled directly each other to configure a closed control loop on behalf of coupling a controller and a plant. the behaviors or this exclusive loop system are expected to be unique in inherent system responses. If its properties be disclosed and generalized well in advance, it is possible for us to use the results for the purpose of fault detection and performance monitoring between control stations from the stage of system design. particularly in such cases as cascade control systems. In this paper. general properties of the proposed system are analyzed firstly to check whether it is controllable and how its steady responses would be. To simplify calculation, the analysis has been performed based on the transfer equation derived from a modelled case which consists of 2 PI controllers and signal converters between them. including time delay element and first-lag element to consider the situation of signal transmission. The results acquired from simulation are suggested to show how it works actually.

• Biomedical Science Letters
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• v.26 no.3
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• pp.149-156
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• 2020

We developed a simple and rapid method for detecting vancomycin resistance genes, such as vanA and vanB, using loop-mediated isothermal amplification (LAMP). To identify not only vancomycin resistance genes, but also the genus Enterococcus, primers were designed for vanA, vanB, and 16S rRNA. Screening for vancomycin susceptibility in Enterococcus was performed using Etest (bioMérieux Inc). The results of the LAMP assay were compared to those of real-time RT-PCR. The optimal conditions for the LAMP assay were 65℃ for 60 min. The detection limits of the LAMP assay for vanA, and vanB were 2 × 10² copies/reaction. Compared to RT-PCR, the sensitivities and specificities of LAMP for 16S rRNA, vanA, and vanB were 100/100%, 100/100%, and 100/100%, respectively. The vanA genotype-vanB phenotype accounted for 57.5% (46/80) of the vancomycin-resistant Enterococci samples collected from 2016 to 2019. In conclusion, the LAMP assay developed in this study showed high sensitivity and specificity for vancomycin-resistant genes. Moreover, due to the simplicity and rapidity of the LAMP assay, its use can be very useful in clinical microbiology laboratories.

• Parasites, Hosts and Diseases
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• v.51 no.5
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• pp.573-577
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• 2013

A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected pigs. The sensitivity of the LAMP assay was compared with that of Q-PCR. Both assays detected T. gondii in the blood of experimentally infected pigs, with 100% agreement. In infected blood samples, the parasite was detected as early as 2 days post-infection and reached a peak in 3-5 days. In 216 field serum samples, the detection rates of LAMP and Q-PCR assays were 6.9% and 7.8%, respectively. This result indicates that the sensitivity of the LAMP assay was slightly lower than that of the Q-PCR assay. However, the LAMP may be an attractive diagnostic method in conditions where sophisticated and expensive equipment is unavailable. This assay could be a powerful supplement to current diagnostic methods.

• The Korean Journal of Mycology
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• v.47 no.4
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• pp.437-441
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• 2019

Verticillium wilt disease is caused by a fungal plant pathogen Verticillium dahliae, which attacks commercial crops such as chrysanthemum. The conventional methods so far used to identify this fungal pathogen require high expertise and are time-consuming. Therefore, in this study, we developed an assay for the rapid and specific detection of V. dahliae infection using loop-mediated isothermal amplification (LAMP) method. For this assay, four primers for LAMP were designed for targeting cellulose-growth-specific protein partial mRNA gene in Verticillium dahliae. Under standard condition, the optimum reaction temperature for amplification is around 60 ℃ within 60 minutes. This LAMP assay was designed to amplify only present in V. dahliae. When this LAMP assay applied to the DNAs for four other soil-borne fungi and host plants, no amplification was detected. Therefore, this LAMP assay we developed for V. dahliae is expected to do detection at the early stage of its infection. The fast and reliable detection method will allow us to develop effective management system to monitor and control infection of this pathogen in chrysanthemum plant.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.29B no.4
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• pp.22-30
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• 1992

A redundant control scheme which can maintain its tracking capability in the case of a controller failure is proposed for the industrial applications which need high reliability with fault-tolerance. It consists of two identical controllers and a switching mechanism which includes failure detection and reconfiguration algorithm. The new detection method against controller failure using fuzzy logic enables the detection of controller failures without failure assumptions through the instability of the failed controller. The failed controller is smoothly removed from the control loop by reducing time weight of the failed controller.

• Animal Bioscience
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• v.36 no.4
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• pp.654-670
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• 2023

Objective: This study aimed to develop loop-mediated isothermal amplification (LAMP) combined with lateral flow dipstick (LFD) and compare it with LAMP-AGE, polymerase chain reaction (PCR), and standard Salmonella culture as reference methods for detecting Salmonella contamination in animal products and animal production environmental samples. Methods: The SalInvA01 primer, derived from the InvA gene and designed as a new probe for LFD detection, was used in developing this study. Adjusting for optimal conditions by temperature, time, and reagent concentration includes evaluating the specificity and limit of detection. The sampling of 120 animal product samples and 350 animal production environmental samples was determined by LAMP-LFD, comparing LAMP-AGE, PCR, and the culture method. Results: Salmonella was amplified using optimal conditions for the LAMP reaction and a DNA probe for LFD at 63℃ for 60 minutes. The specificity test revealed no cross-reactivity with other microorganisms. The limit of detection of LAMP-LFD in pure culture was 3×10² CFU/mL (6 CFU/reaction) and 9.01 pg/μL in genomic DNA. The limit of detection of the LAMP-LFD using artificially inoculated in minced chicken samples with 5 hours of pre-enrichment was 3.4×10⁴ CFU/mL (680 CFU/reaction). For 120 animal product samples, Salmonella was detected by the culture method, LAMP-LFD, LAMP-AGE, and PCR in 10/120 (8.3%). In three hundred fifty animal production environmental samples, Salmonella was detected in 91/350 (26%) by the culture method, equivalent to the detection rates of LAMP-LFD and LAMP-AGE, while PCR achieved 86/350 (24.6%). When comparing sensitivity, specificity, positive predictive value, and accuracy, LAMP-LFD showed the best results at 100%, 95.7%, 86.3%, and 96.6%, respectively. For Kappa index of LAMP-LFD, indicated nearly perfect agreement with culture method. Conclusion: The LAMP-LFD Salmonella detection, which used InvA gene, was highly specific, sensitive, and convenient for identifying Salmonella. Furthermore, this method could be used for Salmonella monitoring and primary screening in animal products and animal production environmental samples.