• Archives of Pharmacal Research
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• 제18권2호
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• pp.133-135
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• 1995

Previously, we reported that the n-butanol fraction of Lonicera japonica showed anti-flammatory activity in mice and rats. Several constituents such a loniceroside A and B, flavonoids and iridoids were isolated from this fraction. In this investigation, the anti-inflammatory activity of the major constituents of L. japonica was studied. Loniceroside A, lonicerin and loganin showed anti-inflammatory activity comparable to aspirin.

• 생약학회지
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• 제53권1호
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• pp.1-7
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• 2022

In the previous study, we reported that luteolin isolated from Lonicera japonica methanolic extract had potent neuroprotective activities in neuronal cell death injured by excessive glutamate. In this study, we tried to confirm the neuroprotective activities of luteolin in glutamate injured HT22 cells and establish mechanisms of neuroprotective action of luteolin. We used HT22 cell death injured by glutamate as a bioassay system. Luteolin decreased reactive oxygen species increased by excessive glutamate treatment in HT22 cells. Also, Ca²⁺ concentration was decreased by luteolin treatment. Luteolin made mitochondrial membrane potential maintain to normal condition. It also increased not only glutathione reductase but also peroxidase to the control level. And it increased amount of glutathione, an endogenous antioxidant. These results suggested that luteolin isolated from L. japonica showed potent neuroprotective activity through the anti-oxidative pathway.

• 대한한방부인과학회지
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• 제33권3호
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• pp.40-59
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• 2020

Objectives: The purpose of this study was to investigate the anti-inflammatory effects of ethanol extracts from Forsythia viridissima Lindley's fructus and Lonicera japonica Thunberg's flos in vitro, which has been frequently used in inflammatory diseases. Methods: In this experiment, the anti-inflammatory effects of ethanol extracts from Forsythia viridissima Lindley's fructus and Lonicera japonica Thunberg's flos were evaluated by checking the following substances of LPS-activated Raw264.7 cell: Prostaglandin E₂ (PGE₂), Nitric oxide (NO), Cyclooxygenase-2 (COX-2), inducible Nitric oxide synthase (iNOS), Interlukine-1β (IL-1β), Interlukine-6 (IL-6), Tumor necrosis factor-α (TNF-α), mitogen-activated protein kinase (MAPK), Inhibitor of kappa B-α (IκBα), Nuclear factor kappa B (NF-κB). And additionally measured reactive oxygen species (ROS) and free radicals to check the antioxidant effect of ethanol extracts from Forsythia viridissima Lindley's fructus and Lonicera japonica Thunberg's flos which affect inflammatory responses. Results: As a result of measuring anti-inflammatory efficacy, PGE2, NO, IL-1β, IL-6, TNF-α production amounts were reduced in the ethanol extracts from Forsythia viridissima Lindley's fructus and Lonicera japonica Thunberg's flos groups compared with the control group, and decreased the amount of COX-2 mRNA, iNOS mRNA gene expression. Expression of MAPK (ERK, JNK, p38) pathway was decreased. Expression of IκBα was increased and NF-κB was decreased. It is demonstrated that ethanol extracts from Forsythia viridissima Lindley's fructus and Lonicera japonica Thunberg's flos, by reducing NF-κB, regulate the expression of the inflammatory genes and reduce the inflammatory mediators. Ethanol extracts from Forsythia viridissima Lindley's fructus and Lonicera japonica Thunberg's flos also decreased ROS production and free radicals, which shown to have antioxidant efficacy and influence anti-inflammatory effects. Conclusions: These data suggest that ethanol extracts from Forsythia viridissima Lindley's fructus and Lonicera japonica Thunberg's flos can be used to treat various inflammatory diseases.

• 대한한의학방제학회지
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• 제30권3호
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• pp.155-163
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• 2022

Objectives : Lonicera japonica is known for anti-inflammation and antibiotic effect in Korean medicine. This study aimed for investigating the cytoprotective effect of Lonicera japonica extract (LJE) for HepG2 cells against arachidonic acid (AA)+iron-induced oxidative stress. Methods : The effect of LJE on cell viability was assessed by MTT assay. ROS assay was selected to assess antioxidant effect of LJE. To assess LJE's effect on mitochondrial function, flow cytometric analysis was operated. And immunoblot analysis was used to establish the underlying mechanism of LJE. Results : LJE protected HepG2 cells against AA+iron-induced oxidative stress by phosphorylation of liver kinase B1 and blocked the decline of procaspase 3. Also, LJE preserved the mitochondrial membrane permeability induced by AA+iron. Conclusion : LJE protected the hepatocyte from AA+iron-induced oxidative stress by activation of LKB1 by the preservation of mitochondrial functions.

• 한국약용작물학회지
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• 제15권5호
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• pp.362-366
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• 2007

This study was conducted to evaluate the status of habitat distribution, environmental characteristics and plant species growing with Lonicera japonica in its habitats. In the distribution of plant coverage below 30%, it was 84.6% in the inland area and 80.7% in the seashore area. There was similar coverage in both inland and seashore areas. But the average relative coverage in the seashore area was 26.1%, which is a little higher than that of the inland area (22.5%). In the habitats, both inland and seashore areas showed a high distribution ratio below 10 degrees. But among them, inland areas showed a high distribution ratio (62.4%) below 10 degrees, which was about twice as much as the seashore areas (32.2%). Habitat distribution was most often found on the southern slopes in both inland and seashore areas. The average soil pH of Lonicera japonica habitat was 5.2 in the inland areas and 6.9 in the seashore areas. Also, the seashore areas showed little content of $P_2O_5$, but high content of K, Mg, Na on the other hand compared to the inland area. Miscanthus sinensis was the highest importance value (16.4) among plants growing with Lonicera japonica in their habitats in both inland and seashore areas. But in the inland areas, Artemisia princeps showed the highest importance value, and Miscanthus sinensis was the highest in the seashore areas.

• 동의생리병리학회지
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• 제19권2호
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• pp.481-489
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• 2005

Lonicerae Flos has antibacterial effects against Staphylococcus aureus, streptococci, pneumococci, Bacillus dysenterii, Salmonella typhi, and paratyphoid. It is an antiviral agent. The herb has a cytoprotective effect against $CCl_{4}-induced$ hepatic injury. It has antilipemic action, interfering with lipid absorption from the gut. Nowadays this herb is used mainly in the treatment of upper respiratory infections, such as tonsillitis and acute laryngitis. It is also used in the treatment of skin suppurations, such as carbuncles, and to treat viral conjunctivitis, influenza, pneumonia, and mastitis. Lonicerae Flos is dried flower buds of Lonicera japonica, L. hypoglauca, L. confusa, or L. dasystyla. But, for the most part, we use whole plant of Lonicera japonica, as a flower bud of it. And, little is known of the original copy of effects of whole plant, except for the 'Bon-Cho-Gang-Mok', which is written the effects of flower of Lonicera japonica are equal to effects of leaves and branch of it. The present study was conducted to evaluate the effect of flower and whole plant of Lonicera japonica on the regulatory mechanism of cytokines, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) for the immunological activities in Raw 264.7 cells. In Raw 264.7 cells stimulated with lipopolysaccharide (LPS) to mimic inflammation, flower and whole plant of Lonicera japonica water extracts inhibited nitric oxide production in a dose-dependent manner and abrogated iNOS and COX-2. Flower and whole plant of Lonicera japonica water extract did not affect on cell viability. To investigate the mechanism by which flower and whole plant of Lonicera japonica water extract inhibits iNOS and COX-2 gene expression, we examined the on phosphorylation of inhibitor ${\kappa}B{\alpha}$ and assessed production of $TNF-{\alpha}$, $interleukin-1{\beta}$ $(IL-1{\beta})$ and interleukin-6 (IL-6). Results provided evidence that flower and whole plant of Lonicera japonica inhibited the production of $IL-1{\beta}$, IL-6 and activated the phosphorylation of inhibitor ${\kappa}B{\alpha}$ in Raw 264.7 cells activated with LPS. These findings suggest that flower and whole plant of Lonicera japonica can produce anti-inflammatory effect, which may play a role in adjunctive therapy in Gram-negative bacterial infections, respectively.

• Environmental Analysis Health and Toxicology
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• 제15권1_2호
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• pp.7-12
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• 2000

Scavenging effects on paraquat induced toxicity were investigated by using methanol (MeOH) and ethylacetate (EtoAC) extracts of Lonicera japonica. The results are summerized as follows: 1. To Fe(III)-ADP-NADPH induced microsomal lipid peroixdation, MeOH and EtoAC extracts showed antioxidative activiies and inhibition ratio at 100 $\mu\textrm{g}$/$m\ell$ 44.4% and 73.8% respectively 2. To microsomal NADPH dependent cytochrome p -450 reductase in rat liver, MeOH and EtoAC extracts inhibited the enzyme activiies and inhibition ratio were 26.3% and 44.8% respectively. 3. Administration (30 mg/kg, iv) of paraquat to rats caused the marked elevation of GOT, GPT, LDH, ALP in the serum and lipid peroxides in the microsome as compared to the control group. Serum GTP, LDH, ALP and liver microsomal LPO were reduced significantaly by administration of MeOH extract. (1,000 mg/kg), EtoAC extract (40 mg/kg) and Silymarin (150 mg/kg) as compared to the paraquat group. From the results, MeOH and EtoAc exuacts. of Lonicera japonica showed the useful scavenger and reducer on the paraquat induced hepatotoxicty.

• Archives of Pharmacal Research
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• 제15권4호
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• pp.365-370
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• 1992

Seven flavonoids were isolated from the aerial parts of Lonicera japonica. Their structures were characterized as hydnocarpin 1, quercetin 2, ochnaflavone 3, ochnaflavone 4'-O-methylether 4, astragalin 5, isoquercitrin 6, and rhoifolin 7 by chemical and spectroscopic evidences.

• 한국응용곤충학회지
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• 제58권3호
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• pp.203-207
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• 2019

노린재목(Hemiptera) 가루이과(Aleyrodidae)에 속하는 담배가루이(Bemisia tabaci Gennadius)는 전 세계적으로 900종 이상의 다양한 작물에 경제적 피해를 주고 있는 주요 해충이다. 특히 이 중 담배가루이 JpL (Lonicera japonica)은 현재까지 일본과 한국에서만 서식하는 종으로 보고되어 있으며, 주로 인동덩굴(Lonicera japonica) 및 일부 식물 종에만 서식하는 것으로 알려져 있다. 본 연구는 2019년 제주도 지역 담배가루이 JpL의 발생 현황 및 확산 정도를 확인하기 위하여 수행되었으며, 총 25개 채집지역 중 24개 지역의 인동덩굴 및 1개 지역의 큰망초(Conyza sumatrensis)에서 담배가루이 JpL 성충 1,003개체를 채집하였다. 채집된 담배가루이 성충의 유전자 염기서열을 분석한 결과, 분석된 모든 담배가 루이는 JpL로 확인되었으며, 본 연구의 결과 담배가루이 JpL의 개체수는 이전에 국내에서 보고된 이후 현재까지 매우 빠른 속도로 증가하고 있음을 확인하였다.

• Mycobiology
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• 제50권2호
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• pp.150-154
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• 2022

Rhytisma lonicericola was identified as a tar spot fungus on Lonicera sp. in 1902, and has since been recorded on several species of Lonicera in China, Japan, and Korea. Most of the previous records of R. lonicericola have been based on a list of disease occurrences in the absence of any formal morphological identification or molecular analyses. Using six newly obtained specimens collected in the past 2 years, we confirmed the tar spot fungus found on L. japonica in Korea as R. lonicericola based on morphological examinations and molecular phylogenetic analyses. This fungus was distinguished from R. xylostei, another tar spot fungus on Lonicera, by ascospore size and geographical distributions. We present detailed mycological information and, for the first time, DNA sequence data useful for the identification of R. lonicericola.