• New & Renewable Energy
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• v.1 no.2 s.2
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• pp.53-59
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• 2005

Objective of the research is to demonstrate solar thermal space and ground heating system which is integrated to a green-house culture facility for reducing healing cost, Increasing the value of product by environment control, and developing advanced culture technology by deploying solar thermal system. Field test for the demonstration was carried out in horticulture complex In Jeju Island. Medium scale solar hot water system was installed in a ground heating culture facility. Reliability and economic aspect of the system which was operated complementary with thermal storage and solar hot water generation were analyzed by investigating collector efficiency, operation performance, and control features. Short term day test on element performance and Long term test of the whole system were carried out. Optimum operating condition and its characteristics were closely Investigated by changing the control condition based on the temperature difference which Is the most important operating parameter For establishing more reliable and optimal design data regarding system scale and operation condition, continuous operation and monitoring on the system need to be further carried out. However, It is expected that, in high-insolation areas where large-scale ground storage is adaptable, solar system demonstrated in the research could be economically competitive and promisingly disseminate over various application areas.

• Development and Reproduction
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• v.24 no.3
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• pp.207-214
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• 2020

Primary cell culture is a sufficient method frequently used to study the cellular properties and mechanisms of isolated cells in a controlled environment. In this study, an embryonic cell line (FGBC8) derived from the blastula stages of embryos of olive flounder Paralichthys olivaceus was developed. Furthermore, conditions for optimal long-term maintenance of this primary embryonic cell culture were investigated. Morphologically, FGBC8 cells were composed primarily of epithelial-like cells. FGBC8 cells were subcultured for >160 passages over ~830 days. The doubling time of FGBC8 cells was 73.8 h, and the modal diploid chromosome number was 48. FGBC8 cells transfected with green fluorescence protein (GFP)-expression plasmid exhibited a strong signal 48 h after transfection. Consequently, we demonstrated that fish serum is a crucial supplement for the long-term survival and maintenance of comparable morphology in these primary embryonic cells. Our results can be used as a guide for primary embryonic cell cultures for other fish species and may be useful for cell biotechnological applications.

• The Journal of the Convergence on Culture Technology
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• v.4 no.1
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• pp.153-163
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• 2018

The purpose of this study was to investigate nurses' ethical dilemma and professionalism in long-term care hospitals. Participants in this study were 210 nurses working in 14 long-term care hospitals. Data analysis was done using SPSS / WIN 24.0 program. As a result of this study, the ethical dilemma of the nurse was moderate. The highest sub-area was 'nurse-patient relationship' and 'respect of life and human rights' was the lowest sub-area. The professionalism of the subjects was moderate, and the score of 'sense of mission' area was the lowest. Nursing ethics guidelines should be developed that reflect the ethical dilemma of nurses in long-term care hospitals, and will provide the right values for the ethical dilemma that nurses face in their workplace. When planning the nurses' professionalism education, it is necessary to have a strategy to enhance the sense of mission by emphasizing beliefs and values about nursing care.

• The Journal of the Convergence on Culture Technology
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• v.6 no.4
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• pp.301-309
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• 2020

The purpose of this study was identify factors influencing dementia nursing performance among long-term hospitals nurses. Participants were 161 long-term hospitals nurses. Data were collected with structured qustionnaires from November 4 from December 13, 2019. Data were analyzed SPSS/WIN 21 program for descriptive statistics, t-test, ANOVA, Pearson's correlation coefficient, and stepwise multiple regression. The significant factors of the dementia nursing performance among long-term hospitals nurses were dementia attitude (β=.280, p=.001), nursing professionalism(β=.237, p=.006), burden form BPSD(β=-.174, p=.012), and dementia education experience(β=.166, p=.014). Therefore, in order to improve dementia nursing performance among long-term care hospital nurses, it is necessary to develop program and apply dementia education to establish a positive dementia attitude and nursing professionalism, and to reduce the burden of dementia behavioral and psychological symptoms.

• The Journal of the Convergence on Culture Technology
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• v.7 no.4
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• pp.237-242
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• 2021

The purpose of this study was to investigate the effect of patient safety competencies and ethical climate in long-term care hospital nurses and to provide the basic data. Data were collected from 120 in D city and analyzed by t-test, ANOVA, Pearson correlation coefficient, and multiple regression using SPSS/WIN 25.0. The degree of Patient Safety Competencies in long-term care hospital nurses was 3.45± .41. There were not significant differences in Patient Safety. There was positive correlation between patient safety competencies and peer(r=.123, p<.001), manager(r=.241, p<.001), and hospital(r=.241, p<.001). The factors affecting the patient safety competencies confidence of the study subjects were peer(β=.23, p=.003), manager(β=.55, p=.004), hospital(β=.43, p=.031), with an explanatory power of 33.5%. Through this research requires the fellow study to determine the factors affecting patient safety competencies confidence of long-term care hospital nurses.

• Journal of The Korea Institute of Healthcare Architecture
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• v.19 no.1
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• pp.9-22
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• 2013

Purpose: The aging population is the quickly increasing in Korea. Since 2008, the National Long term Care Insurance, a number of long term care facilities have established during short time in Korea. Especially, the environmental assessment tool is important for managing healing environment in a long term care facility for the elderly. Alzheimer's Garden Audit Tool (AGAT) is used to assess whether a garden incorporates those elements and qualities as healing outdoor space. In this paper, we discuss the benefits, limitations, and future directions of the assessment tool for long-term care facilities. Methods : The AGAT audit was done through content analysis by 5 experts from diverse discipline as post occupancy evaluation of a long term care facility located in suburban area. The expert group was asked to describe their comments for modification and improvement in application of AGAT. Results : The results of this study show that it is necessary to refine the instrument's items to better meet the needs of the criteria and items for Korean culture-friendly tool. Especially, greenhouse elements (various plants, birds etc.), multisensory experiences, users-oriented space, programmed activities in garden, and hazards on pathway has somewhat limitation as measurement to assess outdoor of long-term care facilities in Korea. Implications : Consequently, AGAT could be applicable to evaluate the outdoor space of long term care facility in Korea with culturally sensitive revision.

• Journal of Embryo Transfer
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• v.29 no.3
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• pp.221-228
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• 2014

Despite that porcine spermatogonial stem cells (pSSCs) have been regarded as a practical tool for preserving eternally genetic backgrounds derived from pigs with high performance in the economic traits or phenotypes of specific human diseases, there were no reports about precise definition of niche conditions promoting proliferation and maintenance of pSSCs. Accordingly, we tried to determine niche conditions supporting proliferation and maintenance of undifferentiated pSSCs for short-term. For these, undifferentiated pSSCs were progressively cultured in different composition of culture medium, seeding density of pSSCs, type of feeder cells and concentration of growth factors, and then total number of and alkaline phosphatase (AP) activity of pSSCs were investigated at post-6 day culture. As the results, the culture of $4{\times}10^5$ pSSCs on mitotically in activated $2{\times}10^5$ STO cells in the mouse embryonic stem cell culture medium (mESCCM) supplemented with 30 ng/ml glial cell line-derived neurotrophic factor (GDNF) was identified as the best niche condition supporting effectively the short-term maintenance of undifferentiated pSSCs. Moreover, the optimized short-term culture system will be a basis for developing long-term culture system of pSSCs in the following researches.

• Journal of the Korean Solar Energy Society
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• v.26 no.3
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• pp.1-8
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• 2006

The objective of this research is to study on the analysis of long-term performance characteristics of various solar thermal system for heating protected horticulture system for reducing heating cost, increasing the value of product by environment control, and developing advanced culture technology by deploying solar thermal system. Long term field test for the demonstration was carried out in horticulture complex in Jeju Island. Reliability and economic aspect of the system which was operated complementary with thermal storage and solar hot water generation were analyzed by investigating collector efficiency, operation performance, and control features. Optimum operating condition and its characteristics were closely investigated by changing the control condition based on the temperature difference which is the most important operating parameter. However, it is expected that, in high-insolation areas where large-scale ground storage is adaptable, solar system demonstrated in the research could be economically competitive and promisingly disseminate over various application areas.

• Journal of Bio-Environment Control
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• v.6 no.2
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• pp.92-96
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• 1997

This study was carried out to clarify the effects of number of branches per pot on the growth and yield in long term bag-culture of sweet pepper. Two plants were grown in pot with four, six, and eight branches. The results are summarized as the followings : 1. Plant height, stem diameter, leaf dry weight, root dry weight, and stem dry weight did not significantly differ among treatments, while the number of leaves and leaf area were the greatest In the treatment with eight branches per pot. 2. Although fruit length was not influenced by the number of branches per pot, fruit diameter and fruit weight were greater with four branches per pot. The yield of the four branches was 124.5ton/ha, which was not significantly different from the yield of the eight branches, 113.4ton/ha. Considering the average fruit weight, fruit quality, and labor saving, four branches per pot appears to be appropriate. 3. Although the yield of eight branches per pot during the first five months was higher, however, four branches per pot should be recommended for the long term bag- culture of sweet pepper because the yield of the four branches from April, which is the middle stage of growth to the final harvest was higher than the yields of the others.

• Animal Bioscience
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• v.36 no.12
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• pp.1889-1897
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• 2023

Objective: 'Cultured meat' has been suggested as means of solving the problems associated with overpopulation and gas emissions. Satellite cells are a major component in the production of cultured meat; however, these cells cannot be maintained in vitro over long periods. Fibronectin is a glycoprotein that affects biological processes such as cell adhesion, differentiation, and migration. Unfortunately, the characteristics of porcine satellite cells grown in a long-term culture when exposed to fibronectin-coated dishes are unknown. The objective of this study was to investigate the appropriate concentration of fibronectin coated dishes for proliferation and maintenance of porcine satellite cells at long-term culture. Methods: In this study, we isolated the satellite cells and fibroblast cells with pre-plating method. We next analyzed the cell doubling time, cell cycle, and rate of expressed paired box 7 (Pax7) and myogenic differentiation 1 (MyoD1) in porcine satellite cells cultured with 20 ㎍/mL of fibronectin-, gelatin-, and non-coated dishes at early and late passage. We then analyzed the proliferation of porcine satellite cells with various concentrations of mixed gelatin/fibronectin. We next determined the optimal concentration of fibronectin that would encourage proliferation and maintenance of porcine satellite cells in a long-term culture. Results: Doubling time was lowest when 20 ㎍/mL of fibronectin was used (as tested during an early and late passage). Levels of expressed Pax7 and MyoD1, assessed using immunocytochemistry, were highest in cells grown using fibronectin-coated dishes. The proliferation of gelatin/fibronectin mixed coatings had no significant effect on porcine satellite cells. The concentration of 5 ㎍/mL fibronectin coated dishes showed the lowest doubling time and maintained expression of Pax7. Conclusion: Fibronectin with 5㎍/mL effectively maintains porcine satellite cells, a discovery that will be of interest to those developing the next generation of artificial meats.