• Plant Biotechnology Reports
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• 제5권1호
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• pp.61-69
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• 2011

The dehydration-responsive element binding proteins (DREB1)/C-repeat (CRT) binding factors (CBF) function as transcription factors and play an important role in agricultural biotechnology and molecular biology studies of drought and freezing stress tolerance. We generated transgenic Lolium perenne plants containing the PCR-cloned Arabidopsis DREB1A/CBF3 gene (AtDREB1A/CBF3) to study the function of this gene construct in drought and freezing tolerance in a species of turfgrass. Compared to the control, AtDREB1A/CBF3 transgenic L. perenne plants showed enhanced drought and freezing stress tolerance. The activities of the enzymes superoxide dismutase (SOD) and peroxidase (POD) were higher in transgenic plants than in the non-transgenic plant control. These results demonstrate that the expression of the AtDREB1A/CBF3 gene in transgenic L. perenne plants enhanced drought and freezing tolerance and that the increased stress tolerance was associated with the increased activities of antioxidant enzymes. These results are relevant to stress biology and biotechnology studies of turfgrass.

• 한국초지학회:학술대회논문집
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• 한국초지조사료학회 1999년도 제24회 정기총회 및 프로그램, 제37회 학술발표회 및 특별강연 초록
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• pp.89-90
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• 1999

페레니얼 라이그라스(Lolium perenne L.)의 질소공급형태에 따른 저장질소의 함량과 재생기간 중 저장질소의 이용 및 재생활력을 구명하고자 수경재배 하였다. (NH$_4$)$_2$SO$_4$, KNO$_3$, Urea, Organic-N 및 NH$_4$NO$_3$의 서로 다른 질소형태로 각각 1mM/week을 4주간 공급하여 건물생산량을 보았고, 예취 후 각각의 질소 형태로 4mM/40days동안 공급하여 흡수된 질소의 함량 및 잔여기관내 축적된 질소의 함량을 관찰하였으며, 예취 후 저장된 질소에 의한 재생활력을 보기 위해 0.2mM NH$_4$NO$_3$의 형태로 동일하게 공급하였다.(중략)

• 한국초지조사료학회지
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• 제20권1호
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• pp.19-24
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• 2000

This study was camed out in order to establish plant regeneration via seed-derived callus of perennialryegrass.Varietal difference in callus growth and plant regeneration was obvious between two cultivars of perennialryegrass. "Reveille" showed a relatively high capacity for plant regeneration. The MS medium was superiorto SH or Bj in callus formation and plant regeneration. The highest regeneration frequency (60%) from calluswas obtained in presence of 5 mg/ l 2,4-D and 1 mg/ t kinetin. Regeneration response varied among calluscultures initiated from the same cultivar. Regeneration frequency was the most effective in 6 weeks-old calliafter initiation and lost their regeneration capacity gradually over a period of 12 weeks.(Key words : Perennial ryegrass (Lolium perenne L.), Plant regeneration)e L.), Plant regeneration)

• 한국초지학회:학술대회논문집
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• 한국초지조사료학회 2001년도 제25회 정기총회 프로그램 제39회 학술발표회 및 특별강연 초록(한국초지학회)
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• pp.83.1-83
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• 2001

• 한국초지학회:학술대회논문집
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• 한국초지조사료학회 2001년도 제25회 정기총회 프로그램 제39회 학술발표회 및 특별강연 초록(한국초지학회)
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• pp.97-97
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• 2001

• 한국초지조사료학회지
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• 제27권4호
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• pp.235-240
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• 2007

페레니얼 라이그래스의 최적 조직배양조건을 확립하기 위하여 성숙종자로부터 배발생 캘러스 유도 및 효율적인 식물체 재분화조건을 체계적으로 확립하였다. 배발생 캘러스 유도시 첨가되는 auxin으로는 2,4-D가 가장 효율적이었으며, 9 mg/L 2,4-D와 0.1 mg/L BA가 첨가된 MS 배지에서 배발생 캘러스가 가장 높은 빈도로 유도되었다. 식물체 재분화는 배발생 캘러스를 1 mg/L 2,4-D와 5 mg/L BA가 첨가된 N6 배지에서 배양했을 때 58.3%의 높은 재분화율을 나타내었다. 본 연구를 통하여 확립된 단기간의 효율적인 재분화 시스템은 분자육종을 통한 신품종 페레니얼 라이그래스 개발에 유용하게 응용되어질 수 있을 것이다.

• 한국초지조사료학회지
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• 제21권2호
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• pp.89-96
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• 2001

Carbohydrate and soluble protein reserves and regrowth characteristics in response to cutting height were investigated over four regrowth cycles of turf-type perennial ryegrass(Lolium perenne L. cv. preludeII). When the plants were at the full-vegetative stage (twelve weeks-old), three sequential defoliations at 3, 6 and 9 cm above the root base were imposed at 2-week intervals. Shoot dry weight in all three treatments continuously decreased with progressing regrowth cycle and the decreasing rate was higher as cutting height was lowered. TNC (total non-structural carbohydrate) in stubble at the end of the fourth regrowth cycle in 3, 6 and 9 cm cutting height decreased by 98%, 82% and 27%, respectively, comparing the initial content. TNC in roots also largely decreased with similar pattern in response to cutting height, whereas the absolute amount was much less compared to stubble. Soluble protein in stubble in 3, 6 and 9 cm cutting height decreased by 98%, 82% and 57%, respectively, at the end of fourth regrowth. A significant correlations between TNC (r=0.906) or protein (r=0.879) at the fourth defoliation and dry weight of regrowing shoots at the end of fourth regrowth were observed. these results indicated that cutting height closely influences the levels of organic reserves available for new growth, and that the levels of reserves might provide a useful tool as a determinant for regrowth dynamics.

• 한국초지조사료학회지
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• 제21권1호
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• pp.15-20
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• 2001

예취한 페레니얼 라이그라스의 재생에 미치는 식물 생장 조절물질의 영향을 조사한 결과, 0.1. 0.5 mg/L의 cytokinin 처리시 가장 높은 재생력을 나타내었으며, kinetin과 2iP가 BAP보다 재생에 보다 좋은 영향을 미쳤다. 2,4-D의 경우, 0.1 mg/L에서 가장 높은 재생력을 나타내었으며, GA$_3$은 초기재생력은 왕성하였으나 예취 2주 이후의 생장이 느린 것으로 나타났다. Cytokinin과 auxin의 세포분열에 미치는 경합을 조사한 결과, kinetin과 2,4-D를 단독으로 처리했을 때보다 두 생장 조절물질을 함께 처리했을 때 더 좋은 재생력을 나타내었다. 페레니얼 라이그라스의 현탁 배양세포에서도 cytokinin 과 auxin을 단독으로 처리했을 때보다 함께 처리했을 때 세포분열이 왕성한 것으로 나타났다.

• 한국초지학회:학술대회논문집
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• 한국초지조사료학회 2001년도 제25회 정기총회 프로그램 제39회 학술발표회 및 특별강연 초록(한국초지학회)
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• pp.88-88
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• 2001

• Plant Resources
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• 제6권2호
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• pp.114-119
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• 2003

In this study, an on-site survey was conducted around Gasado island at Jodo-myeon, Jindo-gun, Jeonnam during July 2 to 25, 2002. The naturalized plants investigated in the surveyed sites were revealed 15 families and 39 species, including Althaea rosea and Oxalis articulata, which are ornamental plants. Only 10 species appeared in Imjado island among the surveyed sites the surveyed sites and Imjado Island, including Elymus sibiricus, Lolium perenne, Chenopodium album, Oxalis articulata, Oxalis corymbosa, Oenothera odorata, Xanthium strumarium, Bidens frondosa, Ambrosia artemisiaefolia var. elatior, and Taraxacum officinale. 6 species were distributed only in Gasado island, Jindo-gun, which included include Festuca myuros, Chenopodium ambrosioides, Amorpha fruiticosa, Malva neglecta, Calendula arvensis, and Carthamus tinctorius. 18 species were showed in Soheuksan island.; Yeosu area, 29 species; Imja Island in Sinan-gun, 34 species, and Gasado Island in Jindo-gun revealed 39 species. It is believed that the species of naturalized plants have been expanded compared to the other areas because of close location to the inland and large population fastiy. The Urbanization Index(UI) of this area was found to be 19.5. The naturalized plant communites in these surveyed sites were classified into Rumex crispus, Elymus sibiricus, and Lolium perenne community according to the vegetation table. Furthermore, they formed a community around areas that received great artificial influence from human dwellings or roads due to the characteristics of species distribution