Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Heterologous expression of the Arabidopsis DREB1A/CBF3 gene enhances drought and freezing tolerance in transgenic Lolium perenne plants

  • Li, Xue (Institute of Turfgrass Science, Beijing Forestry University) ;
  • Cheng, Xiaoxia (Institute of Turfgrass Science, Beijing Forestry University) ;
  • Liu, Jun (Institute of Turfgrass Science, Beijing Forestry University) ;
  • Zeng, Huiming (Institute of Turfgrass Science, Beijing Forestry University) ;
  • Han, Liebao (Institute of Turfgrass Science, Beijing Forestry University) ;
  • Tang, Wei (College of Horticulture and Gardening, Yangtze University)
  • Received : 2010.04.06
  • Accepted : 2010.11.04
  • Published : 2011.01.31
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Abstract

The dehydration-responsive element binding proteins (DREB1)/C-repeat (CRT) binding factors (CBF) function as transcription factors and play an important role in agricultural biotechnology and molecular biology studies of drought and freezing stress tolerance. We generated transgenic Lolium perenne plants containing the PCR-cloned Arabidopsis DREB1A/CBF3 gene (AtDREB1A/CBF3) to study the function of this gene construct in drought and freezing tolerance in a species of turfgrass. Compared to the control, AtDREB1A/CBF3 transgenic L. perenne plants showed enhanced drought and freezing stress tolerance. The activities of the enzymes superoxide dismutase (SOD) and peroxidase (POD) were higher in transgenic plants than in the non-transgenic plant control. These results demonstrate that the expression of the AtDREB1A/CBF3 gene in transgenic L. perenne plants enhanced drought and freezing tolerance and that the increased stress tolerance was associated with the increased activities of antioxidant enzymes. These results are relevant to stress biology and biotechnology studies of turfgrass.

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References

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