• Title/Summary/Keyword: Local reaction

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Improved Fluorometric Assay Method for Ribonuclease Activity

  • Lee, Jong-Soo;Choi, Jong-Soo
    • BMB Reports
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    • v.30 no.4
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    • pp.258-261
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    • 1997
  • A simple quantitative assay method for ribonuclease activity has been developed. This method is based on the decrease of fluorescence intensity emitted by the ethidium bromide bound to RNA due to the degradation of RNA by ribonuclease. The substrate RNA was reacted with ribonuclease A and the fluorescence intensity was measured after the addition of ethidium bromide. The intensity difference was calculated using a blank reaction mixture containing no RNase. Whole cellular RNA substrate produced a significant error and was not suitable for this assay method possibly because of local microheterogeniety caused by high molecular weight rRNA. but satisfying results were obtained with tRNA substrate. The intensity difference increased linearly by raising enzyme concentration up to $2{\times}10^{-4}$ Kunitz Units of ribonuclease A. More refined and reliable results were obtained by use of initial reaction velocities which were calculated from the plots of intensity difference vs time. A linear relationship between initial velocities and enzyme concentrations was observed up to 0.01 Kunitz Units of enzyme.

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Numerical analysis of propagation of macrocracks in 3D concrete structures affected by ASR

  • Moallemi, S.;Pietruszczak, S.
    • Computers and Concrete
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    • v.22 no.1
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    • pp.1-10
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    • 2018
  • In this study an implicit algorithm for modeling of propagation of macrocracks in 3D concrete structures suffering from alkali-silica reaction has been developed and implemented. The formulation of the problem prior to the onset of localized deformation is based on a chemo-elasticity approach. The localized deformation mode, involving the formation of macrocracks, is described using a simplified form of the strong discontinuity approach (SDA) that employs a volume averaging technique enhanced by a numerical procedure for tracing the propagation path in 3D space. The latter incorporates a non-local smoothening algorithm. The formulation is illustrated by a number of numerical examples that examine the crack propagation pattern in both plain and reinforced concrete under different loading scenarios.

A Visualization of the Propane/Air Premixed Flame Interacting with an Ultrasonic Standing-wave by Schlieren Photography (정상초음파가 개재하는 프로판/공기 예혼합화염의 슐리렌기법에 의한 가시화)

  • Lee, Sang Shin;Kim, Jeong Soo;Lee, Do Hyong
    • Journal of the Korean Society of Visualization
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    • v.11 no.1
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    • pp.22-27
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    • 2013
  • An investigation into the influence of ultrasonic standing wave on the structural behavior of propane/air premixed flame has been made to get a clue to the combustion reaction acceleration and combustion instability. Visualization technique utilizing the Schlieren photography was employed for the observation of structural variation of the flame reaction zone. Evolutionary characteristics of the flame front were caught by the high-speed Schlieren image, through which local flame velocity of the moving front were analyzed in detail.

Antiallergic and Anti-inflammatory Effects of Perilla frutescens var. acuta (자소엽의 항알레르기와 항염증효과)

  • You, Jin-Su;Kim, Sang-Yong;Kim, Sang-Hyun;Shin, Tae-Yong
    • Korean Journal of Pharmacognosy
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    • v.43 no.2
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    • pp.163-166
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    • 2012
  • In the present study, we investigated the effect of the water extract of Perilla frutescens var. acuta (Labiatae; WEPF) on the mast cell-mediated allergic reactions. WEPF was anally administered to mice for high and fast absorption. WEPF inhibited compound 48/80-induced systemic allergic reaction. WEPF attenuated immunoglobulin E (IgE)-mediated local allergic reaction. In addition, WEPF decreased the gene expression of pro-inflammatory cytokines in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated HMC-1 cells. These results indicate that WEPF inhibits mast cell-mediated allergic reactions in vivo and in vitro.

Prediction of NOx Formation Characteristics in Turbulent Nonpremixed Hydrogen-Air Jet Flames (비예혼합 수소-공기 난류제트화염내의 NOx 생성특성 예측)

  • Kim, S.K.;Kim, Y.M.;Ahn, K.Y.;Oh, K.S.
    • 한국연소학회:학술대회논문집
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    • 1998.10a
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    • pp.165-170
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    • 1998
  • Turbulent nonpremixed $H_2$-air jet flames are numerically investigated using the joint PDF model. The reaction progress variable is derived by assuming the radicals 0, H, and OH to be in partial equilibrium and additional species $HO_2$ and $H_2O_2$ in steady state. The model is extended to npnadiabatic flame by introducing additional variable for the transport of enthalpy and radiative source term is calculated using a local, geometry independent model. In terms of flame structure and NO formation, the predicted results are favorably agreed with experimental data. The effects of nonequilibrium chemistry and radiative heat loss on the thermal NO formation are discussed in detail.

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A study on the bed combustion of solid waste (고형 폐기물층 연소에 관한 연구)

  • Sin, Dong-Hun;Choe, Sang-Min
    • 한국연소학회:학술대회논문집
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    • 1998.10a
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    • pp.1-8
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    • 1998
  • Waste combustion above a grate is the core process of incineration systems, stability of which should be guaranteed for emission minimization. However, complicated reactions and heat and mass transfer phenomena make understanding the process difficult. One dimensional bed combustor with a numerical combustion model is utilized to investigate the combustion process of the bed, using cubic wood particles as a simulated fuel. Bed combustion behavior is characterized with apparent flame propagation speed, which has close relationship with air supply rate and chemical and physical characteristics of the fuel. Base on the availability of oxygen, two distinct reaction zone is identified; the oxygen-limited and the reaction-limited zone leading to the extinction by excessive convection cooling. The numerical modeling shows good agreement with the experimental results. The transient bed combustion behavior of local temperature and oxygen consumption rate is adequately reproduced. The numerical model is extended to model the waste bed combustion of a commercial incineration plant, which shows meaningful results as well.

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A CASE REPORT OF GIANT CELL REPARATIVE GRANULOMA. (하악골에 발생한 Giant cell Reparative Granuloma의 증례 보고)

  • Park Chang Sik;Lee You Dong
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.4 no.1
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    • pp.59-62
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    • 1974
  • The authors observed, in the routine roentgenographic examination, a rare case of Giant cell Reparative Granuloma found in the mandible of woman 23 years of age who had visited Infirmary of Dental College, Seoul National University' because of the traffic accident. In the serial roentgenograms, Authors had obtained the result as follows; 1. Giant cell Reparative Granuloma occurred below the 20 years of age, and occurred in mandible of female. 2. In roentgenograms, it figures the radiolucent lesion with multilocular apperance. 3. The growing process of Giant cell Reparative Granuloma is not by the neoplastic reaction, but by the local reparative reaction.

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AC Impedance Study of the Electrochemical Behavior of Hydrogen/Oxygen Gas Mixture at Nafion/Catalyst Electrode Interface

  • Song, S.M.;Lee, W.M.
    • Journal of Hydrogen and New Energy
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    • v.11 no.4
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    • pp.179-188
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    • 2000
  • The anodic reaction of hydrogen/oxygen gas mixture at platinum or palladium electrode interfacing with a solid polymer electrolyte was investigated using AC impedance method. The impedance spectrum of the electrode reactions of the mixture depends on the gas composition, electrode roughness, the mode of electrochemical operation and the cell potential. For electrolysis mode of operation, the spectrum taken for the reaction on a rough platinum electrode for the gas mixture revealed clearly that the local anodic reduction of oxygen gas takes place concurrently with the anodic oxidation of hydrogen gas.

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Metal-Organic Vapor Phase Epitaxy III. Atomic Layer Epitaxy (MOVPE 단결정층 성장법 III. 원자층 성장법)

  • 정원국
    • Journal of Surface Science and Engineering
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    • v.23 no.4
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    • pp.197-207
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    • 1990
  • Atomic layer epitaxy is a relatively new epitaxial pprocess chracterized by the alternate and separate exposure of a susbstrate surface to the reactants contaning the constituent element of a compound semicoductror. The ideal ALE is expected to provide sevral advantageous as petcts for growing complicated heterostrutures such as relativly easy controls of the layer thinkness down to a monolayer and in forming abrupt heterointerfaces though monolayer self-saturatio of the growth. In addition, since ALE is stongly dependent on the surface reaction, the growth can also be controlled by photo-excitation which provides activation can be energies for each step of the reaction paths. The local growth acceleration by photo-excitation can be exploited for growing several device strures on the same wafer, which provides another important practical advantage. The ALE growth of GaAs has advanced to the point the laser opertion has been achieved from AlGs/GaAs quantun well structures where thee active layers were grown by thermal and Ar-laser assisted ALE. The status of the ALE growth of GaAs and other III-V compounds will be reviewed with respect to the growth saturation behavior and the electrical properties of the grown crystals.

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Numerical Investigation on the Self-Ignition of High-pressure Hydrogen in a Tube Influenced by Burst Diaphragm Shape (튜브 내 고압 수소의 파열막 형상에 따른 자발 점화 현상에 대한 수치해석)

  • Lee, Hyoung Jin;Kim, Sung Don;Kim, Sei Hwan;Jeung, In-Seuck
    • Journal of the Korean Society of Combustion
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    • v.18 no.3
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    • pp.31-37
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    • 2013
  • Numerical simulations are conducted to investigate the feature of spontaneous ignition of hydrogen within a certain length of downstream tube released by the failure of pressure boundaries of various geometric assumption. The results show that the ignition feature can be varied with the shape of pressure boundary. The ignition at the contact region are developed at the spherical pressure boundaries due to multi-dimensional shock interactions, whereas the local ignition is developed in limited area such as boundary layer at the planar pressure boundary conditions. The spontaneous ignition inside the tube can be generated from the reaction region of only boundary layer regardless of existence of the reaction of core region.