• Journal of Korean Medicine for Obesity Research
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• v.17 no.2
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• pp.101-110
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• 2017

Objectives: In this study, the lipolytic effects of Eriobotrya folium extract (EFE) on local fat was investigated in high fat diet (HFD)-induced obesity mouse and 3T3-L1 adipocytes. Methods: C57BL/6J mice (5 weeks) were fed HFD for 6 weeks to induce obesity. EFE (20 mg/ml, $100{\mu}l$) or saline ($100{\mu}l$) as a normal control was injected into left inguinal fat pad region, 3 times per a week for last 2 weeks. After sacrifice, body weight, and histological changes of the inguinal fat pad were evaluated. The expressions of hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) in inguinal fat pad were analyzed by Western blotting. Also, lipid accumulation and lipases release were determined in 3T3-L1 adipocytes by oil red o staining. Results: EFE significantly reduced the weight of inguinal fat pad and the size of adipocytes in HFD-induced obesity mice compared to control. The treatment of EFE up-regulated the expressions of HSL and ATGL in inguinal fat pads of obesity mice, as well as 3T3-L1 adipocytes. In addition, EFE inhibited the lipid accumulation in 3T3-L1 adipocytes in a dose dependent manner. Conclusions: EFE showed lipolytic effect on local fat of HFD-induced obesity mice by up-regulation of the lipases secretion. This suggests that EFE could be considered as anti-obese substance with lipolytic property on local fat.

• Journal of Acupuncture Research
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• v.30 no.3
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• pp.15-25
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• 2013

Objectives : This study was designed to investigate the anti-obesity effects in local area adipose tissue using by pharmacopuncture of Eriobotryae Folium on mice fed high fat diet. Methods : Male ICR mouse were divided into three groups. Normal diet group (N), High-fat diet(HFD) group, HFD+E(pharmacopuncture of the Eriobotryae Folium) group for 8 weeks. HFD+E group was injected in the concentration of 50 mg/kg once a day for 4 weeks and every other day during the next 4 weeks. We measured body weight, food intake, absolute weight and relative weight in the liver and adipose tissue. Also, we analyzed hematological levels and histopathological changes on adipose tissue. Results : 1. We observed no difference in food intake among three groups. 2. HFD+E group significantly decreased body weight gain and absolute weight and relative weight in the liver and adipose tissue compared with HFD group. 3. Blood AST and ALT were no differences among the experiment groups. However, HFD group was significantly increased higher TG, TC, HDL and LDL than in N group and HDL/LDL ratio was remarkably decreased. On the other hand, HFD+E group was significantly decreased in obesity markers and HDL/LDL ratio was remarkably increased. 4. Levels of adiponectin in serum of HFD+E group was remarkably increased compared with those of HFD group. 5. HFD group was significantly decreased compared with N group in adipocyte number. Compared with the HFD group, HFD+E group was displayed a significant increase by adipocyte number. Conclusions : We suggest that pharmacopuncture of the Leaves of Eriobotrya japonica can play the anti-obesity effects in local area adipose tissue.

• The Korean Journal of Physiology and Pharmacology
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• v.25 no.4
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• pp.333-339
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• 2021

Injection lipolysis or mesotherapy gained popularity for local fat dissolve as an alternative to surgical liposuction. Phosphatidylcholine (PPC) and aminophyl-line (AMPL) are commonly used compounds for mesotherapy, but their efficacy and safety as lipolytic agents have been controversial. Glycerophosphocholine (GPC) is a choline precursor structurally similar to PPC, and thus introduced in aesthetics as an alternative for PPC. This study aimed to evaluate the effects of GPC on adipocytes differentiation and lipolysis and compared those effects with PPC and AMPL using in vitro and in vivo models. Adipogenesis in 3T3-L1 was measured by Oil Red O staining. Lipolysis was assessed by measuring the amount of glycerol released in the culture media. To evaluate the lipolytic activity of GPC on a physiological condition, GPC was subcutaneously injected to one side of inguinal fat pads for 3 days. Lipolytic activity of GPC was assessed by hematoxylin and eosin staining in adipose tissue. GPC significantly suppressed adipocyte differentiation of 3T3-L1 in a concentration-dependent manner (22.3% inhibition at 4 mM of GPC compared to control). Moreover, when lipolysis was assessed by glycerol release in 3T3-L1 adipocytes, 6 mM of GPC stimulated glycerol release by two-fold over control. Subcutaneous injection of GPC into the inguinal fat pad of mice significantly reduced the mass of fat pad and the size of adipocytes of injected site, and these effects of GPC were more prominent over PPC and AMPL. Taken together, these results suggest that GPC is the potential therapeutic agent as a local fat reducer.

• The Journal of the Korean dental association
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• v.41 no.9 s.412
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• pp.637-646
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• 2003

Euccal fat pad is special fat tissue which is different from subdermal fat. Anatomically, buccal fat pad is easy to harvest in the course of dental surgery procedure. In 1802, it was introduced by Bichat, Since Egyedi used buccal fat pad flap for the closure of oro-antral fistula and oro-nasal fistula, it has been widely used as an alternative method for the reconstruction of small to medium-sized intraoral defects in oral and mzxillofacial surgery. Kim et al. reported successful results in the all cases they applied buccal fat pad for the reconstruction of intraoral defect from their 31 months follow-up data. Because intraonal wounds are difficult to complete the layered suture and there are high risks of infection related with wound dental implant surgery, double layer closure using some kind of local flaps or other procedure is recommended. So we are to introduce the useful applications of the pedicled buccal fat pad in the dental surgery procedure from the various case presentations.

• The Korea Journal of Herbology
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• v.28 no.3
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• pp.95-106
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• 2013

Objectives : The researcher investigated the anti-obesity effect of Alismatis Orientale Rhizoma(AP) water extract in mice fed a high fat diet and focused on the analysis of local area adipose tissue. Methods : Male ICR mice were divided into three groups, which were fed either a normal AIN diet, a 45% high fat diet (CT group), or a high fat diet and orally administration with a concentrations of 100 mg/kg (AP100 group) and 300 mg/kg body weight (AP300 group) for eight weeks. Results : As compared with CT group, AP100 group showed significant reductions in absolute weight of liver. As compared with CT group, AP100 group and AP300 group showed significant reductions in weight gain and relative weight of total fat. AST, triglyceride, total-cholesterol levels from the AP 100 group and 300 group were significantly lower than those of the CT, and ALT, LDL-cholesterol levels from the AP 100 group was significantly lower than those of the CT. But serum HDL-cholesterol levels from the AP 100 group and 300 group were significantly higher than those of the CT. And serum adiponectin levels from the AP 100 group was significantly higher than those of the CT. In result of real time PCR, all mRNA expression(PEPCK, G6Pase, PGC-$1{\alpha}$ and $ERR{\gamma}$) of two experimental groups were significantly decreased compared to those of CT group. The treatment with AP on local abdominal area made a fat cell size lessen on the fat tissue in the abdominal cavity and subcutaneous area. Conclusions : These results suggest that AP has an anti-obesity effect and the effect is mediated by inhibition of fat gain.

• The Journal of Korean Physical Therapy
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• v.22 no.6
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• pp.13-19
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• 2010

Purpose: This study was designed to measure the effects of weight, abdominal girth, body fat, abdominal fat and cholesterol levels in combination with electrical stimulation, ultrasound and aerobic exercise on obesity and local lipolysis. Methods: Subjects were 30 obese adults who volunteered to take part in the experiment and had no physical diseases. They were randomly divided into three groups: (1) an aerobic exercise group (n=10), (2) an electrical stimulation group with aerobic exercise (n=10), and (3) an ultrasound stimulation group with aerobic exercise (n=10). Each experimental group went through 8 weeks of training. Results: All measured items including weight, girth of the abdomen, body fat, and cholesterol levels showed significant differences among groups. All three groups showed decreases for all items. The electrical stimulation + aerobic exercise group (group II) showed greater effects than the aerobic exercise group (group I) and the ultrasound stimulation group with aerobic exercise (group III). Conclusion: Electrical stimulation + aerobic exercise and ultrasound stimulation + aerobic exercise cause decreases in weight, girth of the abdomen, body fat and cholesterol level compared to aerobic exercise alone. These methods can be considered to be effective adjuvants to aerobic exercise in obese adults.

• Archives of Plastic Surgery
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• v.36 no.6
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• pp.685-690
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• 2009

Purpose: Autologous fat graft is a widely accepted technique used for soft tissue augmentation. Nonetheless, the use of fat graft is limited by unpredictable survival rates and repeated grafting. To avoid repeated grafting, cryopreserved fat graft technique has recently been widely used. On the other hand, the number of patients with chronic infection(who received cryopreserved fat injection) has currently been increasing. Therefore, this study was focused on the safety of cryopreserved fat injection from the infection. Methods: We collected 150 samples from local aesthetic clinics to examine the safety of cryopreserved autologous fat. To test for microbacterial contaminations of the cryopreserved fat specimens, microbacterial cultures & antibiotics sensitivity tests were performed. Then, we examined possible correlation between the preservation period and donor sites, focused on the results of microbacterial culture. Results: Cultures were positive for Staphylococcus epidermidis in 5 samples(methicillin - resistant Staphylococcus epidermidis in 4 samples), Micrococcus species in 3 samples. An average duration of preservation was 191 days and there was no significant correlation between the duration of preservation and microbacterial growth. Conclusion: Staphylococcus epidermidis was the leading cause of cryopreserved fat contamination, and the resistance to methicillin is common. Based on the above results, aseptic handling of fat during harvesting and preservation appeared to be most important.

• Journal of Korean Medicine for Obesity Research
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• v.6 no.2
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• pp.75-83
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• 2006

Objectives : This study was performed to identify the effects of high frequency therapy on localized obesity. Methods : This trial was carried out in 12 volunteers. Volunteers were divided into 3 groups; upper arm group (n=4), thigh group (n=4) and abdomen group (n=4) according to local obesity type. Body weight and body fat were measured by Inbody 720 and CT (Computed Tomography) immediately before and following high frequency therapy. Diathermy was performed twice a week for 4 weeks for a total of 8 treatments. Results : In the upper arm group, body weight, body fat mass, fat area by CT scan and circumference were increased after treatment but not significantly (p>0.05). In the thigh group, body weight, body fat mass and circumference were decreased and fat area by CT scan was increased but both not significantly (p>0.05). In the abdomen group, significant differences were not found despite decreases in body weight, body fat mass, visceral fat and subcutaneous fat after diathermy (p>0.05). Conclusions : There was no significant effects of high frequency therapy on localized obesity.

• Journal of radiological science and technology
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• v.40 no.2
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• pp.205-211
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• 2017

There is a need for aggressive diagnosis and treatment in middle-aged and high-risk individuals who are more likely to progress from nonalcoholic fatty liver to hepatitis. In this study, nonalcoholic fatty liver was divided into severe, moderate, and severe, and classified by quantitative method using computer analysis of GLCM algorithm. The purpose of this study was to evaluate the characteristics of ultrasound images in the local fat avoidance region. Normal, mild, moderate, severe fatty liver, and focal fat sparing area, 80 cases, respectively. Among the parameters of the GLCM algorithm, the values of the Autocorrelation, Square of the deviation, Sum of averages and Sum of variances with high recognition rate of the liver ultrasound image were calculated. The average recognition rate of the GLCM algorithm was 97.5%. The result of local fat avoidance image analysis showed the most similar value to the normal parenchyma. Ultrasonography can be easily accessed by primary screening, but there may be differences in the accuracy of the test method or the correspondence of results depending on proficiency. GLCM algorithm was applied to quantitatively classify the degree of fatty liver. Local fat avoidance region was similar to normal parenchyma, so it could be predicted to be homogeneous liver parenchyma without fat deposition. We believe that GLCM computer image analysis will provide important information for differentiating not only fatty liver but also other lesions.

• Animal Bioscience
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• v.36 no.10
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• pp.1558-1567
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• 2023

Objective: Dietary green cabbage was evaluated for its impact on fatty acid synthetic ability in different adipose tissues during fattening of Wanxi White geese. Methods: A total of 256 Wanxi White geese at their 70 days were randomly allocated into 4 groups with 4 replicates and fed 0%, 15%, 30%, and 45% fresh green cabbage (relative to dry matter), respectively, in each group. Adipose tissues (subcutaneous and abdominal fat), liver and blood were collected from 4 birds in each replicate at their 70, 80, 90, and 100 days for fatty acid composition, relative gene expression and serum lipid analysis. Two-way or three-way analysis of variance was used for analysis. Results: The contents of palmitic acid (C16:0), palmitoleic acid (C16:1), linoleic acid (C18:2), and alpha-linolenic acid (C18:3) were feeding time dependently increased. The C16:0 and stearic acid (C18:0) were higher in abdominal fat, while C16:1, oleic acid (C18:1), and C18:2 were higher in subcutaneous fat. Geese fed 45% green cabbage exhibited highest level of C18:3. Geese fed green cabbage for 30 d exhibited higher level of C16:0 and C18:0 in abdominal fat, while geese fed 30% to 45% green cabbage exhibited higher C18:3 in subcutaneous fat. The expression of Acsl1 (p = 0.003) and Scd1 (p<0.0001) were decreased with green cabbage addition. Interaction between feeding time and adipose tissue affected elongation of long-chain fatty acids family member 6 (Elovl6), acyl-CoA synthetase longchain family member 1 (Acsl1), and stearoly-coA desaturase 1 (Scd1) gene expression levels (p = 0.013, p = 0.003, p = 0.005). Feeding time only affected serum lipid levels of free fatty acid and chylomicron. Higher contents of C16:0, C18:1, and C18:3 were associated with greater mRNA expression of Scd1 (p<0.0001), while higher level of C18:2 was associated with less mRNA expression of Scd1 (p<0.0001). Conclusion: Considering content of C18:2 and C18:3, 30% addition of green cabbage could be considered for fattening for 30 days in Wanxi White geese.