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http://dx.doi.org/10.15429/jkomor.2017.17.2.101

Effect of Eriobotrya folium on Local Fat via Regulation of Lipase Secretion  

Lee, Woonkyeu (Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University)
Choi, You Yeon (Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University)
Yang, Woong Mo (Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University)
Publication Information
Journal of Korean Medicine for Obesity Research / v.17, no.2, 2017 , pp. 101-110 More about this Journal
Abstract
Objectives: In this study, the lipolytic effects of Eriobotrya folium extract (EFE) on local fat was investigated in high fat diet (HFD)-induced obesity mouse and 3T3-L1 adipocytes. Methods: C57BL/6J mice (5 weeks) were fed HFD for 6 weeks to induce obesity. EFE (20 mg/ml, $100{\mu}l$) or saline ($100{\mu}l$) as a normal control was injected into left inguinal fat pad region, 3 times per a week for last 2 weeks. After sacrifice, body weight, and histological changes of the inguinal fat pad were evaluated. The expressions of hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) in inguinal fat pad were analyzed by Western blotting. Also, lipid accumulation and lipases release were determined in 3T3-L1 adipocytes by oil red o staining. Results: EFE significantly reduced the weight of inguinal fat pad and the size of adipocytes in HFD-induced obesity mice compared to control. The treatment of EFE up-regulated the expressions of HSL and ATGL in inguinal fat pads of obesity mice, as well as 3T3-L1 adipocytes. In addition, EFE inhibited the lipid accumulation in 3T3-L1 adipocytes in a dose dependent manner. Conclusions: EFE showed lipolytic effect on local fat of HFD-induced obesity mice by up-regulation of the lipases secretion. This suggests that EFE could be considered as anti-obese substance with lipolytic property on local fat.
Keywords
Eriobotryae folium; Local fat; Obesity; Hormone-sensitive lipase; Adipose triglyceride lipase;
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