Journal of Korean Medicine for Obesity Research (한방비만학회지)

The Society of Korean Medicine for obesity Research (한방비만학회)
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Effect of Eriobotrya folium on Local Fat via Regulation of Lipase Secretion

비파엽추출물의 지방분해효소 조절을 통한 국소 지방분해 효능

  • Lee, Woonkyeu (Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University) ;
  • Choi, You Yeon (Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University) ;
  • Yang, Woong Mo (Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University)
  • 이운규 (경희대학교 한의과대학 융합한의과학교실) ;
  • 최유연 (경희대학교 한의과대학 융합한의과학교실) ;
  • 양웅모 (경희대학교 한의과대학 융합한의과학교실)
  • Received : 2017.11.06
  • Accepted : 2017.11.27
  • Published : 2017.12.30
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Abstract

Objectives: In this study, the lipolytic effects of Eriobotrya folium extract (EFE) on local fat was investigated in high fat diet (HFD)-induced obesity mouse and 3T3-L1 adipocytes. Methods: C57BL/6J mice (5 weeks) were fed HFD for 6 weeks to induce obesity. EFE (20 mg/ml, $100{\mu}l$) or saline ($100{\mu}l$) as a normal control was injected into left inguinal fat pad region, 3 times per a week for last 2 weeks. After sacrifice, body weight, and histological changes of the inguinal fat pad were evaluated. The expressions of hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) in inguinal fat pad were analyzed by Western blotting. Also, lipid accumulation and lipases release were determined in 3T3-L1 adipocytes by oil red o staining. Results: EFE significantly reduced the weight of inguinal fat pad and the size of adipocytes in HFD-induced obesity mice compared to control. The treatment of EFE up-regulated the expressions of HSL and ATGL in inguinal fat pads of obesity mice, as well as 3T3-L1 adipocytes. In addition, EFE inhibited the lipid accumulation in 3T3-L1 adipocytes in a dose dependent manner. Conclusions: EFE showed lipolytic effect on local fat of HFD-induced obesity mice by up-regulation of the lipases secretion. This suggests that EFE could be considered as anti-obese substance with lipolytic property on local fat.

Keywords

References

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