• Natural Product Sciences
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• 제8권4호
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• pp.177-182
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• 2002

The effect of aqueous extract of Sanguisorba officinalis L. (Rosaceae) root (SOAE) on the immediate-type allergic reactions by anal therapy was investigated. SOAE (0.01 to 1 g/kg) dose-dependently inhibited systemic anaphylaxis induced by compound 48/80 in mice. When SOAE was pretreated at the same concentrations with systemic anaphylaxis, the plasma histamine levels were reduced in a dose-dependent manner. SOAE (0.1 and 1 g/kg) also significantly inhibited local anaphylaxis activated by anti-DNP IgE. SOAE (0.001 to 1 mg/ml) dose-dependently inhibited the histamine release from rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-DNP IgE. The level of cyclic AMP (cAMP) in RPMC, when SOAE (1 mg/ml) was added, transiently and significantly increased compared with that of basal cells. These results provide evidence that anal therapy of SOAE may be beneficial in the treatment of allergic diseases.

• 소성∙가공
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• 제28권5호
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• pp.247-256
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• 2019

In this study, the development of annealing textures in cold rolled and annealed tantalum sheets was analyzed using electron backscatter diffraction. At $900^{\circ}C$, the textures of the recrystallized grains in the partially and completely recrystallized microstructures displayed significant similarities. The average diameter of the recrystallized grains with ${\gamma}-fiber$ orientations exceeded that of grains with different orientations, and the average growth rates were unrelated to the orientations after an initial stage of recrystallization. Additional cold rolling and annealing was done for controlled initial microstructures and textures inherited from various processes of prior cold rolling and annealing. This second cycle of the process resulted in stronger textures with major ${\gamma}-fiber$ orientations as a result of the enhanced ${\gamma}-fiber$ orientations in the preceding textures. A coarse-grained prior microstructure resulted in a weaker annealing texture than a fine grained one regardless of the stronger previous texture, which was occasioned by the sub-structures of the minor orientations at local deformation inhomogeneities such as sharp in-grain shear bands.

• 생명과학회지
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• 제28권2호
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• pp.201-206
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• 2018

지리오갈피의 소비를 촉진시키고 지역 특산품을 개발할 목적으로 지리오갈피 발효주의 제조하여 그 특성 등을 검토하였다. 지리오갈피의 뿌리, 줄기 및 열매를 원료미의 10%(W/W)씩 첨가하여 $25^{\circ}C$, 13일 동안 발효시킨 결과 각각 알코올 함량이 $14.7{\pm}0.7%$, $15.3{\pm}0.9%$ 및 $15.5{\pm}0.7%$의 발효주를 얻었다. 발효주의 pH는 pH 3.9~pH 4.4, 총산은 0.72~0.75%를 나타내어 3시험구간의 차이는 거의 없었다. 총당 함량은 발효 24시간부터 급격히 떨어져 발효 13일에는 7.1~7.5%를 나타내었다. 총 유리아미노산은 지리오갈피의 뿌리와 열매를 첨가한 시험구 보다 줄기를 첨가한 시험구에서 7,045.01 ppm으로 가장 높게 검출되었다. 발효주 중의 acanthoside-D의 함량을 측정한 결과 10%의 뿌리 및 열매로 제조한 발효주에서는 35.42 ppm 및 18.91 ppm이 검출되었으나 줄기로 제조한 발효주에서 57.06 ppm의 높은 함량을 나타내었다.

• 한국동물생명공학회지
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• 제35권2호
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• pp.177-182
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• 2020

The present study aimed to investigate the conceptus-related changes during early pregnancy in the Black Bengal breed of goat. A total of 22 gravid genitalia of the Black Bengal goats were collected from local slaughterhouses. The crown-rump lengths (CRL) of the conceptuses were determined to estimate the gestational age (GA). The length and diameter of uterine horn and amniotic sac were measured, and volume of amniotic and allantoic fluid formed by individual conceptus were recorded. The results reveal that the CRL is positively correlated with GA of the conceptus (R² = 0.89, p < 0.05); however, CRL was not influenced by number of conceptus. Both the left and the right uterine horn gradually increased in size with the advancement of pregnancy irrespective of conceptus number present in the horn. The size of the amniotic sac of conceptus gradually increased with the conceptus age but maintained spherical shape from 5 to 7 weeks of pregnancy. The amniotic fluid formed by individual conceptus rapidly increased from 5 weeks (3.4 ± 0.3 mL) to 7 weeks (21.0 ± 2.0 mL) and 9 weeks (111.5 ± 4.0 mL). The volume of allantoic fluid formed by individual conceptus was steadily increased until 7 weeks (60.0 ± 5.0 mL) and began to decline slowly thereafter (50.0 ± 5.0 mL at 9 weeks). Notably, there was no effect of conceptus number per pregnancy on individual amniotic and allantoic fluid volume. The cotyledons have first appeared on the allanto-chorionic surface from 4 to 5 weeks of pregnancy. The closed eye, nostril and hooves of the conceptus became visible at 7 weeks of pregnancy. The present study has shown the basic information on conceptus-related developmental changes during early pregnancy up to 9 weeks in Black Bengal goat.

• 생명과학회지
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• 제28권10호
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• pp.1140-1146
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• 2018

이중탈인산화효소(DUSP)는 성장인자활성 단백질키나제(MAPK)를 조절해서 세포성장과 분화에 관여하며 암, 당뇨병, 면역질환, 신경질환의 신약개발표적이다. DUSP 단백질군에 속하는 DUSP19는c-Jun N-말단 키나제(JNK)를 조절하며 골관절염의 질환화과정에 관여한다. 우리는 야생형 DUSP19 에 비하여 상당히 활성이 증가된 cavity 형성 돌연변이인 DUSP19-L75A의 결정구조를 규명하였다. 결정구조는 Leu75의 곁가지가 없어진 결과로 cavity가 잘 형성되어 있는 것을 보여주며, 활성부위에 결합한 황이온이 회전된 형태로 존재하는 것을 보여준다. Cavity 형성에도 불구하고 cavity를 둘러싸고 있는 잔기들은 그다지 재조정되지 않은 것으로 나타나며 그 대신에 멀리 떨어진 트립토판 잔기가 소수성결합을 강화하고 있는 것으로 나타나서 L75A 돌연변이의 접힘은 cavity 부위의 재조정이 아니라 글로벌 접힘 에너지 최소화 기작에 의해 안정화 되었음을 발견할 수 있었다. 회전된 활성화부위 황이온의 구조는 인산화티로신 잔기와 유사함이 발견되어 L75A 돌연변이가 최적의 활성화형태를 유도했다는 것을 알 수 있었다. 내부 cavity에 의한 활성증가현상과 이에 대한 구조적 정보는 DUSP19의 알로스테릭 조절과 치료제 개발에 정보를 제공한다.

• 한국측량학회지
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• 제26권5호
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• pp.493-504
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• 2008

SBAS(Space-Based Augmentation System)는 광역보정항법시스템(WADGPS, Wide Area DGPS)으로써 지역 보정항법시스템(LADGPS, Local Area DGPS)의 단점을 보완한 위성항법 보정시스템이다. 이 연구에서는 GPS 측위의 대표적인 방법인 실시간 이동측위(RTK; Real-Time Kinematic), DGPS(Differential GPS) 측위, 단독측위(Standalone)와 SBAS 이동측위를 실시하고 그에 따른 측위 정확도 비교를 통해 SBAS 이동측위의 성능을 분석하였다. 정지측위 결과 산출한 좌표를 참값으로 간주하였고, 각각의 측위방법으로 획득한 관측데이터를 참값과 비교하여 계산한 2차원 평균제곱근(RMS: Root Mean Square)오차와 3차원 RMS 오차를 정확도 비교의 지표로 사용하였다. 그 결과, 각 측위방법에 따른 3차원 RMS 오차는 RTK 측위 13.1cm, DGPS 측위 126.0cm, 이중주파수 단독측위 135.7cm, 단일주파수 단독측위 428.9cm, 그리고 SBAS 측위 109.2cm로 나타났다. 이를 통해 SBAS 측위의 정확도가 DGPS 측위 정확도와 대등한 수준임을 확인할 수 있었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.365-365
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• 2017

Yogurt is a food produced by bacterial fermentation of milk and the bacteria used to make it are known as "yogurt cultures". Most of them belong to probiotics such as Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus bacteria. Domestic fermented milk market is increasing and about 30 companies are producing yogurt. The purpose of this study was to analyze the quality characteristics of domestic commercial semisolid type yogurt. We collected 20 types of commercial yogurt at local markets. Physicochemical properties including pH, sugar content, acidity, viscosity and microbial characteristics of lactic acid bacteria counts were measured. The yogurt showed pH 4.5, 7.4~18.1% of sugar contents, 0.6~1.3% of total acids and 282~748 cP of viscosities. In the microorganism populations, lactic acid bacteria count were 6.5~11.5 Log CFU/mL and anaerobic lactic acid bacteria count were 7.2 ~ 11.1 Log CFU/mL. The quality characteristics were different depending on the constituents of the sample and the microorganisms used. These results are related to the quality characteristics of yogurts which are useful information about identifying new trends in domestic fermented milk industry.

• Archives of Plastic Surgery
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• 제43권5호
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• pp.424-429
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• 2016

Background The objective of this paper was to describe a novel technique for improving the maintenance of nipple projection in primary nipple reconstruction by using acellular dermal matrix as a strut in one of three different configurations, according to the method of prior breast reconstruction. The struts were designed to best fill the different types of dead spaces in nipple reconstruction depending on the breast reconstruction method. Methods A total of 50 primary nipple reconstructions were performed between May 2012 and May 2015. The prior breast reconstruction methods were latissimus dorsi (LD) flap (28 cases), transverse rectus abdominis myocutaneous (TRAM) flap (10 cases), or tissue expander/implant (12 cases). The nipple reconstruction technique involved the use of local flaps, including the C-V flap or star flap. A $1{\times}2-cm$ acellular dermal matrix was placed into the core with O-, I-, and L-shaped struts for prior LD, TRAM, and expander/implant methods, respectively. The projection of the reconstructed nipple was measured at the time of surgery and at 3, 6, and 9 months postoperatively. Results The nine-month average maintenance of nipple projection was $73.0%{\pm}9.67%$ for the LD flap group using an O-strut, $72.0%{\pm}11.53%$ for the TRAM flap group using an I-strut, and $69.0%{\pm}10.82%$ for the tissue expander/implant group using an L-strut. There were no cases of infection, wound dehiscence, or flap necrosis. Conclusions The application of an acellular dermal matrix with a different kind of strut for each of 3 breast reconstruction methods is an effective addition to current techniques for improving the maintenance of long-term projection in primary nipple reconstruction.

• 대한수의학회지
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• 제29권4호
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• pp.503-515
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• 1989

Critical parameters affecting sensitivity and specificity of an enzyme-linked immunosorbent assay(ELISA) for detection of antibodies to avian infections bronchitis virus(IBV) were standardized. By adopting the optimized conditions an equation calculating ELISA antibody titers from the observations at single serum dilution was formulated. The purified antigen of IBV Mass-41 strain was dispensed into polystyrene microplate wells at a concentration of 300ng per well($100{\mu}l$) and the plates were coated by completey drying at $37^{\circ}C$. Diluted chicken serum and horseradish peroxidase conjugated goat anti-chicken IgG were added in order in $100{\mu}l$ volumes per well and allowed to react for 30 minutes each at room temperature. Just before use and after each reaction the plates were washed three times with distilled water. Finally o-phenylenediamine solution was added as an enzyme substrate. After incubation for another 15 minutes at room temperature absorbances were read at 492nm. Hyperimmune serum against Mass-41 strain was used as internal reference positive(IRP) serum. After repeated titration of IRP and negative serum, a constant titer of IRP was determined. Serum titrations were carried out for various sample sera together with IRP and negative sera and the observed titers of sample sera were corrected by reflecting the ratio between observed and constant titers of IRP serum. These corrected titers of the sample sera were plotted against sample/positive(S/P) OD ratios. All the OD's measured in the serum titrations were also corrected by substracting negative serum OD. The following equation was formulated from the above data; $Log_{10}$ ELISA titer=$5.568({\log}_{10}S/P)+4.161$ Thus it was possible to calculate ELISA titer by measuring absorbance at 1/400 single serum dilution. Titer measured by cross ELISA tests employing Mass-41 strain and three local IBV isolates were similar. These results suggest that the ELISA tests standardized in this study can be used for evaluating not only vaccinal immunity but also for infection status against fields IBV's.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2000년도 추계학술대회
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• pp.87-90
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• 2000

The hydrogeochemical and isotopic studies on deep groundwater in the Munkyeong area, Kyeongbuk province were carried out. $CO_2$-rich groundwater (Ca-HC $O_3$ type) is characterized by low pH (5.8~6.5) and high TDS (up to 2,682 mg/L), while alkali groundwater (Na-HC $O_3$ type) shows a high pH (9.I~10.4) and relatively low TBS (72~116 mg/L). $CO_2$-rich water may have evolved by $CO_2$ added at depth during groundwater circulation. This process leads to the dissolution of surrounding rocks and Ca, Na, Mg, K and HC $O_3$ concentrations are enriched. The low Pc $o_2$ (10$^{-6.4}$atm) of alkali groundwaters seems to result from the dissolution of silicate minerals without a supply of $CO_2$. The $\delta$$^{18}$ O and $\delta$D values and tritium data indicate that two types of deep groundwater were both derived from pre-thermonuclear meteoric water. The carbon Isotope data show that dissolved carbon in the $CO_2$-rich water was possibly derived from deep-seated $CO_2$ gas. The $\delta$$^{18}$ S values of dissolved sulfate show that sulfate reduction occurred at great depths. The application of various chemical geothermometers on $CO_2$-rich groundwater shows that the calculated deep reservoir temperature is about 130~175$^{\circ}C$. Based on the geological setting, water chemistry and environmental isotope data, each of the two types of deep groundwater represent distinct hydrologic and hydrogeochemical evolution at depth and their movement is controlled by the local fracture system.m.