• Journal of Periodontal and Implant Science
• /
• v.47 no.2
• /
• pp.66-76
• /
• 2017

Purpose: Oral wound healing requires gingival fibroblasts to respond to local growth factors. Epigenetic silencing through DNA methylation can potentially decrease the responsiveness of gingival fibroblasts to local growth factors. In this study, our aim was to determine whether the inhibition of DNA methylation sensitized gingival fibroblasts to transforming growth factor-${\beta}1$ (TGF-${\beta}1$). Methods: Gingival fibroblasts were exposed to 5-aza-2'-deoxycytidine (5-aza), a clinically approved demethylating agent, before stimulation with TGF-${\beta}1$. Gene expression changes were evaluated using quantitative polymerase chain reaction (PCR) analysis. DNA methylation was detected by methylation-sensitive restriction enzymes and PCR amplification. Results: We found that 5-aza enhanced TGF-${\beta}1$-induced interleukin-11 (IL11) expression in gingival fibroblasts 2.37-fold (P=0.008). 5-aza had no significant effects on the expression of proteoglycan 4 (PRG4) and NADPH oxidase 4 (NOX4). Consistent with this, 5-aza caused demethylation of the IL11 gene commonly next to a guanosine (CpG) island in gingival fibroblasts. The TGF-${\beta}$ type I receptor kinase inhibitor SB431542 impeded the changes in IL11 expression, indicating that the effects of 5-aza require TGF-${\beta}$ signaling. 5-aza moderately increased the expression of TGF-${\beta}$ type II receptor (1.40-fold; P=0.009), possibly enhancing the responsiveness of fibroblasts to TGF-${\beta}1$. As part of the feedback response, 5-aza increased the expression of the DNA methyltransferases 1 (DNMT1) (P=0.005) and DNMT3B (P=0.002), which are enzymes responsible for gene methylation. Conclusions: These in vitro data suggest that the inhibition of DNA methylation by 5-aza supports TGF-${\beta}$-induced IL11 expression in gingival fibroblasts.

• The Korean Journal of Emergency Medical Services
• /
• v.25 no.2
• /
• pp.19-38
• /
• 2021

Purpose: This study aimed to investigate the relationship between emergency medical service accessibility in different regions and the sudden death rate in elderly patients with ischemic heart disease using data analysis techniques and suggest improvements in regional emergency medical services. Methods: The study collected data from the NEDIS database and Statistics Korea. Data on a total of 75,867 patients aged ≥65 years were reviewed among patients with ischemic heart disease who visited emergency medical institutions in 2018. Frequency analysis, chi-square test, multiple logistic regression analysis, and simple logistic regression analysis were performed using SPSS PC Window 25.0. Results: With an emergency medical resource per 100km², there was a concomitant reduction in the risk of death. There was a decrease in the death rate by 0.967, 0.970, 0.997, and 0.391 times with the increase in the presence of a fire department, an ambulance, a paramedic, and a regional medical center, respectively. Furthermore, a decrement in the death rate was witnessed 0.844, 0.825, and 0.975 times with the initiation of a local emergency medical center, a local emergency medical institution, and an angiography device, respectively(p <.001). Conclusion: To improve the accessibility of emergency medical services, the population and geometric area of the region should be considered essential factors when deploying emergency medical resources.

• Archives of Plastic Surgery
• /
• v.50 no.1
• /
• pp.30-36
• /
• 2023

Background Most children with facial lacerations require sedation for primary sutures. However, sedation guidelines for invasive treatment are lacking. This study evaluated the current status of the sedation methods used for pediatric facial laceration repair in Korea. Methods We surveyed one resident in each included plastic surgery training hospital using face-to-face interviews or e-mail correspondence. The health care center types (secondary or tertiary hospitals), sedation drug types, usage, and dosage, procedure sequence, monitoring methods, drug effects, adverse events, and operator and guardian satisfaction were investigated. Results We included 45/67 hospitals (67%) that used a single drug, ketamine in 31 hospitals and chloral hydrate in 14 hospitals. All health care center used similar sedatives. The most used drug administered was 5 mg/kg intramuscular ketamine (10 hospitals; 32%). The most common chloral hydrate administration approach was oral 50 mg/kg (seven hospitals; 50%). Twenty-two hospitals (71%) using ketamine followed this sequence: administration of sedatives, local anesthesia, primary repair, and imaging work-up. The most common sequence used for chloral hydrate (eight hospitals; 57%) was local anesthesia, administration of sedatives, imaging work-up, and primary repair. All hospitals that used ketamine and seven (50%) of those using chloral hydrate monitored oxygen saturation. Median operator satisfaction differed significantly between ketamine and chloral hydrate (4.0 [interquartile range, 4.0-4.0] vs. 3.0 [interquartile range, 3.0-4.0]; p <0.001). Conclusion The hospitals used various procedural sedation methods for children with facial lacerations. Guidelines that consider the patient's condition and drug characteristics are needed for safe and effective sedation.

• Journal of dental hygiene science
• /
• v.15 no.6
• /
• pp.728-735
• /
• 2015

This research was based on using status and factors associated in public health centers survey in 703 local residents from September 1 to 25, 2015. Data were analyzed with chi-square test, t-test, one way ANOVA, and Stepwise Multiple Regression analysis using SPSS WIN 21.0 program and significance level was set at p<0.05. It was investigated that Incheon city health centers' utilization of residents were 38.8%, the annual average of utilization was 3.92 times. Health centers were mainly used by themselves (25.3%) or recommendation of the people around them (17.8%). The reason why they don't use was no need (27.0%) and don't know well (9.4%) or long distance (7.8%). The average of awareness about the total health programs was 41.2%, the average of utilization rate was 8.1%, both higher rate of awareness and utilization about health programs were vaccination (86.6%, 36.9%), and health examinations (67.2%, 18.7%). The average of satisfaction about health programs was 3.88 out of 5, sealant (4.26 points) and rationing fluoride solution (4.07 points) indicated a high level of satisfaction. The most common factor which represents the impact on public health use and use frequency were the time it takes to move, also gender, age and form of home ownership were major factor in common. Based on this, we have to seek concrete measures of national health programs which conducted to improve the health of local residents can be implemented successfully.

• Safety and Health at Work
• /
• v.7 no.4
• /
• pp.317-321
• /
• 2016

Background: This study was conducted with aim of providing an overview of the current status of occupational health services and identifying the most common harmful agents at workplaces of Iranian self-employed enterprises (Nano-Scale Enterprises). Methods: A cross-sectional study was performed among a random sample including 1,758 employees engaging in self-employed enterprises with 5 and less employees. Results: Coverage of occupational health surveillance was very poor, annual health examinations were been conducted only for 64 (3.64%) of males and 31 (1.76%) of females, and occupational health trainings were not included of the services at all. Personal Protective Equipment were available in 462 (26.3%) of the enterprises. only in 0.4% of the enterprises working processes were been equipped by a local exhaust ventilation system. Difficult postures were the most common (81.5%) adverse working conditions. Conclusion: This study revealed a poor level of the implementation of occupational health services in Iranian self-employed enterprises. Based on the findings, providing basic training on the occupational health, more enforcing in conduction of health examinations and providing PPE, and taking appropriate strategies aimed at eliminating or minimizing work environment harmful agents are the major factor that should be considered to improve the level of occupational health services among the studied enterprises.

• Journal of Food Hygiene and Safety
• /
• v.31 no.1
• /
• pp.15-20
• /
• 2016

Malachite green was measured in 200 freshwater fish collected from local markets in Seoul using HPLC-DAD and LC-MS/MS. LC-MS/MS method was validated by linearity, accuracy, precision and limits of detection and quantification according to the CODEX's recommendation and HPLC-DAD method was applied according to the Food Code. Malachite green levels above the quantification limit of the LC-MS/MS were determined 18.5% (37) but just 1 fish was shown to contain malachite green by HPLC-DAD. Of 83 domestic fish, 21 fish were detected malachite green (25.3%). Of 117 fish from China, just 16 fish were detected malachite green (13.4%). In detection rate by species carp (35.0%), Crucian carp (30.4%), cat fish (28.0%), Korean bull head (23.8%), snake head (20.0%), eel (10.5%) and loach (7.8%) were in order. Especially, fish collected at summer were shown to contain malachite green frequently; the detection rate was 54.8%.

• Proceedings of the Korean Society of Medical Physics Conference
• /
• 2003.09a
• /
• pp.72-72
• /
• 2003

Purpose: To prove feasibility of proton chemical shift imaging (lH CSI) during stereotactic procedure, authors performed IH CSI in combination with a stereotactic headframe and selected targets according to local metabolic information, evaluated the pathologic results. Methods: The 1H CSI directed stereotactic biopsy was performed in five patients. 1H CSI was performed before conventional stereotactic MRI with gadolinium enhancement for stereotactic coordinates. The metabolite images expressed as integral ratios, Cho/Cr and Lac/Cr, were displayed in different colors. The stereotactic target coordinates were correlated with the coordinates from the 1H CSI images. Results: The final pathologic results obtained were concordant with the local metabolic information from 1H CSI. We believe that 1H CSI-directed stereotatic biopsy has the potential to significantly improve the accuracy of stereotactic biopsy targeting. Conclusions : Metabolic signals derived from 1H CSI could give us more direct clues for stereotactic target selection during the subsequent conventional stereotactic MR imaging. 1H CSI was feasible with the stereotatic headframe in place. The final pathologic results obtained were concordant with the local metabolic information from 1H CSI. Acknowledgement: This study was supported by a grant of the Center for Functional and Metabolic Imaging Technology, Ministry of Health & Welfare, Republic of Korea (02-PJ3-PG6-EV07-0002).

• Journal of The Korea Institute of Healthcare Architecture
• /
• v.17 no.4
• /
• pp.15-23
• /
• 2011

Long-term care Insurance system for the Elderly was started to respond to the aged society on July 2008 in Korea. So responsibility of the care for the old is being changed from the family to the society. The introduction of that insurance system has brought the growth of demand for care service. To improve the level of quality for care service providers in long-term care, the role of care worker in providers is recognized as important. But welfare system for the care worker seems to be poor. So the Seoul Metropolitan Council is preparing an ordinance for the care worker and the purpose of this study is for the ordinance as well. Questionnaires, interviews, documentary surveys were used to carry out this study. The results of this study are summarized as follows. The job stability is the most important thing for the care worker, so local government has to make efforts to promote the job stability of care worker. A welfare center for care workers is needed for the health for care workers in the local government. And the local government has to supervise service providers more strictly and evaluate them in treatments of care worker.

• Journal of The Korea Institute of Healthcare Architecture
• /
• v.25 no.3
• /
• pp.25-36
• /
• 2019

Purpose: To establish guidelines for local public hospitals, the survey was conducted primarily on the current status of local public hospitals, focusing on the zone configuration and the spatial area of the operating department. Methods: 3 Methods have been used in this paper. 1) Analyzing drawings to derive the net floor area of each room. 2) A literature survey about composition of the zone by infection control. 3) Observation survey for user circulation analysis. Results: The operation department consists of the major, secondary, support and connected zones according to the user's actions. The spatial area composition ratio for each zone is major zone: 41.79%, secondary zone: 10.87%, support zone: 23.10%, and connected zone: 24.24%. Implications: This study is a basic research for guideline for the operation department in local public hospitals. In this study, zones configuration of the operation departments are classified in terms of user actions and room functions. In the future, it is required to establish criteria considering infection control and copper connection aspects.

• The Journal of Korean Society for School & Community Health Education
• /
• v.16 no.2
• /
• pp.115-130
• /
• 2015

Objectives: This study aimed to review the relationship between parenting daily hassles (PDHs), parenting burden and maltreatment as perceived by Korean and Japanese mothers raising children. In addition, the influence of parenting support, efficiency and satisfaction on child maltreatment were also reviewed. Methods: Data were gathered through self-administered questionnaires given to the mothers using the childcare center in both countries. As for the final procedure, 820 Korea and 700 Japan mothers were statistically processed, and data were analyed through factor analysis and path analysis. Result: The results showed that in both countries, parenting burden caused by PDHs significantly affected physical and mental maltreatment. parenting efficiency and satisfaction as a buffering factor could inhibit mental maltreatment. However, among buffering factors, Korean parenting mothers chose parenting efficiency and Japanese parenting mothers did parenting satisfaction. Conclusions: Based on the findings, it is needed parenting mothers are offered on opportunity to take health education programs and to understand their child's behaviors and learn parent's roles of parenting. In addition, local community support is desired.