• Asian Pacific Journal of Cancer Prevention
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• 제14권9호
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• pp.5467-5472
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• 2013

Objective: To construct short hairpin RNA (shRNA) eukaryotic expression vectors targeting Livin and Survivin genes, and to explore the impact of co-transfection of Livin and Survivin shRNA expression vectors on the biological behavior of HepG2 cells. Methods: shRNA eukaryotic expression vectors pSD11-Livin and pSD11-Survivin were designed and constructed then transfected into HepG2 cells separately or in combination. mRNA and protein expression in transfected cells was assessed by quantitative fluorescence PCR and Western blotting, respectively. Cell proliferation was measured by MTT assay and cell apoptosis by TUNEL assay. Results: The Livin and Survivin shRNA eukaryotic expression vectors were successfully constructed and transfected into HepG2 cells. The relative mRNA expression levels of Livin and Survivin in HepG2 cells co-transfected with pSD11-Livin and pSD11-Survivin were $0.12{\pm}0.02$ and $0.33{\pm}0.13$, respectively, which was significantly lower than levels in cells transfected with either pSD11-Livin or pSD11-Survivin (P<0.05). The relative protein expression levels of Livin and Survivin in the co-transfected cells were also significantly decreased compared to single-transfection (P<0.05). The inhibition rate of cell growth in the co-transfection group was higher than that in the single-transfection groups at 48 h, 60 h, or 72 h after transfection (P<0.01). The apoptotic rate increased to the greatest extent in the co-transfection group relative to any other group (P<0.05). Conclusions: Co-transfection with pSD11-Livin and pSD11-Survivin was more efficient than transfection with either vector alone in reducing the mRNA and protein expression of Livin and Survivin genes in HepG2 cells. Co-transfection also inhibited the proliferation of transfected cells more than the other groups, and induced cellular apoptosis more effectively.

• Asian Pacific Journal of Cancer Prevention
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• 제13권6호
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• pp.2681-2685
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• 2012

Objectives: This study focused on PTEN and Livin expression and associations with malignancy in human renal clear cell carcinomas (RCCC). Methods: PTEN and Livin expression was assessed in 100 RCCC tissue samples, 50 paracarcinoma cases, and 20 normal renal tissue samples using the immunohistochemical Streptavidin proxidase (SP) method. The relationships between binding and corresponding biological characteristics, such as histological grade, lymph node metastases, and clinical stages were analyzed. Results: Positive PTEN expression in RCCC was significantly lower than in renal tissue adjacent to carcinoma tissue and normal renal tissue (P<0.01). Livin expression in the renal tissue adjacent to the carcinoma and normal renal tissues exhibited only low levels, whereas overall Livin expression in RCCC was statistically significant (P<0.01). In RCCC, PTEN expression rate gradually decreased with an increase in clinical stage, whereas that of Livin increased to statistically significant levels (P<0.01), PTEN and Livin levels being negatively correlated (r=-0.395, P<0.01). Conclusions: PTEN and Livin are important in RCCC development. The two factors combined are expected to provide indices for estimating RCCC malignancy and progression levels, as well as references for RCCC diagnosis and treatment.

• Asian Pacific Journal of Cancer Prevention
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• 제15권20호
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• pp.8577-8579
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• 2014

Osteosarcoma is a common malignant tumor of bone, but mechanisms underlying its development are still unclear. At present, it is believed that the inhibition of normal apoptotic mechanisms is one of the reasons for the development of tumors, so specific stimulation of tumor cell apoptosis can be considered as an important therapeutic method. Livin, as a member of the newly discovered inhibitor of apoptosis proteins (IAPs) family, has specifically high expression in tumor tissues and can inhibit tumor cell apoptosis through multiple ways, which can become a new target for malignant tumor treatment (including osteosarcoma) and might of great significance in the clinical diagnosis of tumors and the screening of anti-tumor agents and carcinoma treatment.

• 광산업정보
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• 통권53호
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• pp.16-17
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• 2009

• IMMUNE NETWORK
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• 제2권3호
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• pp.137-141
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• 2002

Among the members of the inhibitor of apoptosis (IAP) protein family, only Livin and survivin have been reported to have variant forms. We have found a variant form of c-IAP2 through the interaction with the X protein of HBV using the yeast two-hybrid system. In contrast to the wild-type c-IAP2, the variant form has two stretches of sequence in the RING domain that are repeated in the C-terminus that would disrupt the RING domain. We demonstrate that the variant form has an inhibitory effect on TNF-mediated $NF-{\kappa}B$ activation unlike the wild-type c-IAP2, which increases TNFmediated $NF-{\kappa}B$ activation. These results suggest that this variant form has different activities from the wild-type and the RING domain may be involved in the regulation of TNF-induced $NF-{\kappa}B$ activation.

• Asian Pacific Journal of Cancer Prevention
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• 제14권9호
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• pp.4943-4952
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• 2013

Hepatocellular carcinoma (HCC) is one of the most common malignancies, with a very poor prognosis. Despite significant improvements in diagnosis and treatment in recent years, the long-term therapeutic efficacy is poor, partially due to tumor metastasis, tecurrence, and resistance to chemo-or radio-therapy. Recently, it was found that a major feature of tumors is a combination of unrestrained cell proliferation and impaired apoptosis. There are now 8 recogized members of the IAP-family: NAIP, c-IAP1, c-IAP2, XIAP, Survivin, Bruce, Livin and ILP-2. There proteins all contribute to ingibition of apoptosis, and provide new potential avenues of cancer treatment. As a powerful tool to suppress gene expression in mammalian cells, RNAi species for inhibiting IAP genes cab be directed against cancers. This review will provide a brief introduction to recent developments of the application IAP-siRNA in tumor studies, with the aim of inspiring future treatment of HCC.

• 한국학교보건학회지
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• 제16권1호
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• pp.135-148
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• 2003

The purpose of this study was to find out the degree of satisfaction of body image and sex-role identity of primary school boys and girls and to examine their relevance between two sex groups. The data from study would be useful for mapping out some measures for school health education. Two hundred and forty five(245) students of 6th grade from 5 different primary schools in Seoul city and Gyonggi Province were selected for the study. The meaningful difference among demographic sociological characteristics between boys and girls has been shown to be in religion($x^2$=6.705, p=.044), the order of siblings($x^2$=7.455, p=.024), mother's academic career ($x^2$=6.705, p=.035) and the levels of living ($x^2$=4.108, p=.043). In the frequency distribution of sex-role identity, boys and girls were revealed as : undifferentiated 41.0%, 36.4%; as androgynous 24.8%, 25.8% respectively. Boys have masculinity (23.1%), on the other hand girls more feminity (22.7%). The meaningful difference of the degree of satisfaction of body image depends on father's ag(t=3.029, p=.050), mother's occupation(t=2.878, p=.023), home atmosphere(t=9.056, p=.000), level of livin(t=2.216, p=.028), academic achievements(t=4.580, p=.011). Masculine sex-role identity has shown its meaningful difference in mother's academic background(t=7.042, p=.001), academic achievements(t=14.963, p=.000). Feminine sex-role identity according to general characteristics shows meaningful difference in gender(t=2.683, p=.008), the order of siblings(t=3.019, p=0.51), father's academic career(t=5.558, p=.004) mother's academic background(t=7.676, p=.001), mother's occupation (t=3.405, p=.010), home atmosphere(t=4.492, p=.012), school performance(t=16.774, p=.000). Sex-role identity influences on the degree of satisfaction of body image as the relation bet ween the degree of satisfaction of body image and sex-role identity is pro correlative.

• 융합보안논문지
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• 제17권3호
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• pp.59-64
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• 2017

우리나라는 고령화 사회 문제와 동반하여 1인 가구 고령자의 급격한 증가세를 보이고 있어, '독거노인 u-Care 서비스'라는 정책을 통해 문제해결을 위한 노력을 하고 있다. 본 연구는 혼자 사는 고령자의 건강과 안전을 위한 u-Care 서비스의 사용 환경 개선을 위한 새로운 기술 적용을 통해 좀 더 나은 u-Care 서비스 개선방안을 제안하고자 하였다. 첫째, 사물인터넷 기술 적용을 통해 1인 가구 고령자를 위한 u-Care서비스의 개선이다. 기존의 센서를 통한 안전 관련 상황정보 수집 분석과 함께 웨어러블 디바이스를 통해 건강정보를 측정하고, 건강정보 데이터를 활용하여 원격 모니터링 서비스를 제공하여 스마트폰과 같은 개인 디바이스를 이용해 의료기관에 사용자 자신의 건강상태를 전송해 향후 원격 의료 상담 또는 진료까지도 연결할 수 있을 것이다. 둘째, 감성서비스 로봇기술의 적용을 통해 단순한 안전과 건강관리뿐만 아니라 1인 가구 고령자의 정서적 안정감을 고려를 통한 u-Care서비스의 개선이다. 로봇과의 상호관계 형성이 가능한 사용자 환경의 제공은 정서적 측면에서 긍정적 효과를 제공할 수 있을 것이고, 기존의 u-Care서비스 제공 서비스에 향후 돌봄 기능 서비스제공을 통해 1인 가구 고령자의 독립적 일상생활에도 도움을 줄 수 있을 것으로 기대된다.