• 한국축산식품학회지
• /
• 제25권4호
• /
• pp.494-499
• /
• 2005

본 연구에서는 유아의 분변에서 분리한 항산화 효과를 갖는 유산균인 Lactobacillus gasseri KACC 91155의 Jurkat T cell line에서의 지질 과산화 억제 효과, 세포 보호 효과 및 DNA 손상 억제 효과를 측정하였다. 그 결과 1. gasseri 91155는 세포 산화에 의해 생성된 지질 과산화물인 MDA의 생성을 방지하였으며, 또한 뚜렷하게 세포 생존성을 증가시켰다. 그리고 산화적 손상으로부터 DNA 손상을 줄여주는 효과를 나타내었다.

• 유기물자원화
• /
• 제27권2호
• /
• pp.19-30
• /
• 2019

본 연구는 국내산 우각이 혼합된 유기질비료를 개발하여 가장 많은 유기재배 면적을 차지하고 있는 벼를 포함하여 가지에 대한 시용효과를 조사하고 수입 유박을 대체할 유기자원으로 우각의 활용가능성을 구명하고자 실시하였다. 질소함량이 높은 국내산 유기자원 선발을 위하여 계분, 어분, 콩깻묵, 참깻묵, 들깻묵, 혈분, 우각, 맥주오니 등 8종을 분석하여 질소함량이 높은 유기자원을 선발하였고 보조제로 왕겨 바이오차, 미강 등을 원료별, 혼합비율별로 혼합하고 성분을 분석하여 유기농업에 사용 가능한 유기질비료 제조조건을 확립하였다. 우각은 전질소(T-N) 함량이 12.0 %로 높아 혈분 13.5 % 다음으로 높았으며 어분 및 깻묵은 전질소 함량이 5.9~7.9 % 수준이었다. 계분은 유기농업에 사용가능한 무항생제 산란계 계분을 사용하였으며, 맥주오니는 질소함량이 3.4 %로 나타났다. 무항생제 계분, 우각, 맥주오니 등을 주재료로 바이오차, 미강 등을 보조제로 사용하여 유기질비료를 제조한 결과. 수입유박의 질소함량(4.0~4.2 %) 대비 개발한 유기질비료의 질소함량은 7.5 %로 높고 중금속함량은 Zn 400 mg/kg, Cu 120 mg/kg 이하 등으로 나타나 질소 함량이 높고 유기농업자재 품질기준에 적합한 유기질비료를 개발하였다. 우각이 포함된 유기질비료를 사용하여 벼와 가지를 재배하면서 시용효과를 조사한 결과 토양검정질소시비량 기준 100 % 시용시 혼합유박 대비 시용량을 40 % 감소하였음에도 벼 생육 및 수량이 대등하였으며, 가지 재배시에도 동일한 결과를 나타내었다. 우각 등 국산 유기자원을 이용한 새로운 고농도 질소원 선발 및 이를 이용한 유기재배 적합 유기질비료 개발은 친환경농업 확대 보급의 중요성이 높아지고 있는 현 시점에서 지역자원을 이용한 기존 수입 혼합유박 대체 연구의 출발점이자 폐기되고 있는 국내 유기자원의 활용 방안 모색에서 큰 의미를 가지며 향후 확대 보급된다면 친환경농산물의 안정적 생산에 기여할 수 있을 것으로 기대된다.

• Journal of Animal Science and Technology
• /
• 제44권6호
• /
• pp.661-668
• /
• 2002

축산기술연구소 대관령지소에서 사육된 한우 암소 118두에 대해 산유량 및 비유곡선을 추정하고자 하였다. 분만 후 1, 2, 3 및 4개월의 일일 평균 산유량은 3.74${\pm}$0.96kg, 3.64${\pm}$0.97kg, 3.26${\pm}$1.15kg 및 2.99${\pm}$1.08kg 이었으며 4개월간의 일일 평균 산유량은 3.52${\pm}$0.84kg이었다. 일반적인 비유곡선의 상수 추정치는 A(분만직후 첫유량)가 2.4845, b(상향경사도)가 0.1734 및 c(하향경사도)가 0.0060이었다. 최고 추정유량은 3.75kg이었고 최고 유량 도달시기는 29.03일이었는데 초산우 보다는 경산우가 최고 추정 유량이 높고 최고 유량 도달시기가 늦어지는 것으로 나타났으며 봄철 분만우가 가을철 분만우에 비해 최고 추정 유량이 높고 최고 유량 도달시기가 늦어지는 것으로 나타났으며 봄철 분만우가 가을철 분만우에 비해 최고 추정 유량이 높고 최고 유량 도달시기도 빨라지는 경향을 보였다.

• 한국가축번식학회지
• /
• 제27권4호
• /
• pp.317-324
• /
• 2003

The present study were performed to analysis the hematocrit and the red blood cells content into the blood plasma of the transgenic pigs harboring recombinent human erythropoietin gene (rhEPO). Mouse whey acidic protein (mWAP) linked to rhEPO gene was microinjected into pronuclei of porcine one-cell zygotes. After delivered of offspring, PCR analyses identified one mWAP-rhEPO transgenic founder offspring(F$_{0}$). The first generation of transgenic pig (F$_{0}$) harboring mWAP-hEPO appeared to be a male, and the second generation (F$_1$) pigs were made by natural mating of F$_{0}$ with domestic swine, and male and female transgenic pigs (F$_1$) were identified by PCR. The blood samples from transgenic and normal pigs were collected for 50 days during lactation and were counted the red blood cell (RBC) numbers and Hematocrit (HCT) content into the blood. The transgenic pigs expressing rhEPO in their blood gave rise to higher RBC numbers and HCT contents than control animals. rhEPO was secreted both in the blood and milk of genetically engineered pigs harboring rhEPO gene. Therefore, this study provides a model regarding the production of transgenic pig carrying hEPO transgene for biomedical research.earch.

• Asian-Australasian Journal of Animal Sciences
• /
• 제18권2호
• /
• pp.296-300
• /
• 2005

Human-induced soil salinity is becoming a major threat to agriculture across the world. This salinisation occurs in both irrigated and rain-fed agricultural zones with the highest proportions in the arid and semi-arid environments. Livestock can play an important role in the management and rehabilitation of this land. There are a range of plants that grow in saline soils and these have been used as animal feed. In many situations, animal production has been poor as a result of low edible biomass production, low nutritive value, depressed appetite, or a reduction in efficiency of energy use. Feeding systems are proposed that maximise the feeding value of plants growing on saline land and integrate their use with other feed resources available within mixed livestock and crop farming systems. Salt-tolerant pastures, particularly the chenopod shrubs, have moderate digestible energy and high crude protein. For this reason they represent a good supplement for poor quality pastures and crop residues. The use of salt-tolerant pasture systems not only provides feed for livestock but also may act as a bio-drain to lower saline water tables and improve the soil for growth of alternative less salt tolerant plants. In the longer term there are opportunities to identify and select more appropriate plants and animals for saline agriculture.

• 한국농촌생활과학회지
• /
• 제7권1호
• /
• pp.69-80
• /
• 1996

The purpose of this study is to offer basic data of education to improve farm lives though investigating rural homemakers' life style. The results of this study are as follows : 1. The life structure of rural homemakers was classified into two structures as a material resources and human resources. The life style of rural homemakers was classified into systematical life style, traditional value style, social active style, and progressive active style. 2. The life styles of rural homemakers according to social population variance were as follows. The lower of age, homemakers whose family incomes more 30 million won or below 10 million won, who has religions, who employed in livestock were showed at very systematical life style. The homemakers who live in mountain village, higher the much age becomes, and the education level become lower, have showed traditional value style. Rural homemakers the lower of age, the higher incomes, composed of single parent ＋ children who remain single were appeared social active style. Rural homemakers the lower of age, the more shortened farm life periods, who employed in livestock were appeared progressive active style. 3. Systematical life style was more affected by material resources structure, traditional value style was affected by material resources. And social active style was more affected by human resources, progressive active style was affected by human resources.

• 한국토양비료학회지
• /
• 제42권5호
• /
• pp.379-384
• /
• 2009

본 연구에서는 바이오매스자원별 실태 및 동태적 변화를 알아보기 쉽게 시간적 공간적인 분포와 추세를 시각화하여 주제별 분포지도를 작성하였다. 분뇨 배출량은 축종별 사육두수에 따른 원단위 배출량을 적용하여 계산하였다. 전국 가축분뇨배출량 지도를 작성하여 분석해본 결과 경기남부 지역과 충청남도 북부 지역에서 분뇨배출량이 많이 나타나는 것을 알 수 있었고, 시군구 단위의 조사에서도 비슷한 결과가 나타났다. 또한 음식물 폐기물 폐수발생량, 음식물 쓰레기 매립, 재활용 분포지도에서는 수도권지역이 공공시설 및 민간시설 모두 음식물 폐기물 폐수발생량이 높게 나타났고, 민간시설 부분에서는 경상도 일원이 폐수발생량이 높았다. 강원도, 전남 지역에서는 상대적으로 음식물 쓰레기 재활용 분포도가 높게 나타났다. 바이오매스자원으로 활용가치가 높은 농업부산물인 볏짚 생산량 분포도를 시 군 단위로 작성하였는데 볏짚 생산량이 많은 곡창 지역의 경우 바이오에너지로 활용하는 방안을 모색할 필요가 있다고 판단된다. 이들 결과를 통해 바이오매스 발생원별 분포지도는 정책입안자, 농민, 일반인에게 바이오매스 자원에 대한 정보를 가시적으로 전달할 수 있고, 친환경 농업정책 추진과 바이오 에너지 이용 정책 자료로 활용 가능할 것으로 생각된다.

• BMB Reports
• /
• 제39권2호
• /
• pp.183-188
• /
• 2006

Using the Phred/Phrap/Polyphred/Consed pipeline established in the National Livestock Research Institute of Korea, we predicted candidate coding single nucleotide polymorphisms (cSNPs) from 7,600 expressed sequence tags (ESTs) derived from three cDNA libraries (liver, M. longissimus dorsi, and intermuscular fat) of Hanwoo (Korean native cattle) steers. From the 7,600 ESTs, 829 contigs comprising more than two EST reads were assembled using the Phrap assembler. Based on the contig analysis, 201 candidate cSNPs were identified in 129 contigs, in which transitions (69%) outnumbered transversions (31%). To verify whether the predicted cSNPs are real, 17 SNPs involved in lipid and energy metabolism were selected from the ESTs. Twelve of these were confirmed to be real while five were identified as artifacts, possibly due to expressed sequence tag sequence error. Further analysis of the 12 verified cSNPs was performed using the program BLASTX. Five were identified as nonsynonymous cSNPs, five were synonymous cSNPs, and two SNPs were located in 3'-UTRs. Our data indicated that a relatively high SNP prediction rate (71%) from a large EST database could produce abundant cSNPs rapidly, which can be used as valuable genetic markers in cattle.

• Reproductive and Developmental Biology
• /
• 제30권1호
• /
• pp.27-34
• /
• 2006

This study was conducted to compare the reproduction ability of the wild type boar and recombinant human erythropoietin (hEPO) transgenic boar semen. Ejaculated boar semen was analyzed by flow cytometry, Elisa and IVF methods. In experiment 1, flow cytometric analysis showed that the live sperm ratio of transgenic boar sperm significantly lower (P<0.05) than that of wild type boar after incubation at 20, 22, 24 and 26 hr. In experiment 2, the presence and levels of various cytokines (IL-6, IL-10 and $TNF-{\alpha}$) to related animal reproduction in the seminal and blood plasma were examined using specific enzyme immunoassay. There was no significant difference between both groups. In experiment 3, the fertilizing capacity and developmental ability of both boar sperm were compared. The transgenic boar sperm had a significantly low capacity of penetration, sperm-zona binding, embryo development, and blastocyst formation compared to wild type sperm (P<0.05). These results suggest that transgenic boar sperm harboring hEPO gene has low sperm viability than wild type boar, and it is a reason to decrease of fertility and litter size.

• Journal of Animal Science and Technology
• /
• 제65권3호
• /
• pp.511-518
• /
• 2023

This study examined the association between functional sequence variants (FSVs) of myosin heavy chain 3 (MYH3) genotypes and collagen content in a Landrace and Jeju native pig (JNP) crossbred population. Four muscles (Musculus longissimus dorsi, Musculus semimembranosus, Musculus triceps brachii, and Musculus biceps femoris) were used for the analysis of meat collagen content, and the same animals were genotyped for the FSVs of the MYH3 gene by using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). Three FSVs of MYH3 genotypes were identified and had genotype frequencies of 0.358, 0.551, and 0.091 for QQ, Qq, and qq, respectively. QQ animals for the FSVs of the MYH3 genotypes showed higher collagen content in their M. longissimus dorsi (p < 0.001), M. semimembranosus (p < 0.001), M. triceps brachii (p < 0.001), and M. biceps femoris (p < 0.001) than qq homozygous animals. After the validation of this result in other independent populations, the FSVs of MYH3 genotypes can be a valuable genetic marker for improving collagen content in porcine muscles and can also be applied to increase the amount of collagen for biomedical purposes.