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The Antioxidant Effect of Lactobacillus gasseri KACC 91155 Isolated from Korean Infant in Jurkat T Cells  

Jeong Seok-Geun (National Livestock Research Institute, RDA)
Kim Hyun-Soo (National Livestock Research Institute, RDA)
Ham Jun-Sang (National Livestock Research Institute, RDA)
Chae Hyun-Seok (National Livestock Research Institute, RDA)
Lee Jong-Moon (National Livestock Research Institute, RDA)
Ahn Chong-Nam (National Livestock Research Institute, RDA)
Publication Information
Food Science of Animal Resources / v.25, no.4, 2005 , pp. 494-499 More about this Journal
Abstract
In the present study, we investigate the protective effect of antioxidant strain Lactobacillus gasseri KACC 91155, isolated from Korean infant feces(Obstetrics & Gynecology, Suwon, Korea) on the oxidative stress damage on the Jurkat T cells. To estimate the extent of cellular lipid peroxidation inhibition, MDA(malondialdehyde) production was measured Furthermore, cell viability was detected by the MTT assay, DNA damage was tested by the comet assay. Cell grown in medium with or without L gasseri lysate$(100\~1,000{\mu}g)$ were treated with $H_2O_2,\;Fe^{2+}$ as an oxidative stimulus. From the result obtained, the supplementation of Jurkat T cells with L. gasseri lysate significantly decreased in MDA production (1,250 vs. 835 nmol/mg protein), and DNA damage(31.6 vs. 22.6 tail moment). Also L gasseri increase cell viability against oxidative damage. We concluded that the L. gasseri KACC 91155 showed a protective effect against oxidative stress.
Keywords
Lactobacillus gasseri; antioxidant; Jurkat T cell; DNA damage;
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