• Journal of the Korean Society of Food Science and Nutrition
• /
• v.26 no.3
• /
• pp.494-500
• /
• 1997

This study was conducted to investigate the effect of acorn extracts on the antioxidative enzyme system. Male Sprague-Dawley rats(110$\pm$10g) were fed on containing normal and high fat diets. They were orally administrated (0.02g/100g B.W) of acorn ethylacetate-extract and water-extract at the same time once a day, respectively. Net weight gain and feed efficiency ratio were increased in high fat diet groups and decreased by acorn extracts administration. The effect of acorn extracts on hepatic glutathione peroxidase(GSH-Px), glutathione S-transferase(GST) and catalase activities did not show significancy in normal fat diet groups. GST and catalase activities and lipid peroxidation content(LPO) were significantly increased in high fat diet groups and this increment were decreased by acorn extracts administration. However GSH-Px activity and GSH content were decreased in high fat diet groups but increased by acorn extracts administration. The activities of lactate dehydrogenase(LDH), alkaline phosphatase(ALP) and aminotransferase in serum were significantly increased in high fat diet group but these increment reduced in acorn extracts administration groups. These results indicate that acorn extracts could improve the liver function and prevent the metabolic diseases by hyperlipidemia.

• Toxicological Research
• /
• v.13 no.4
• /
• pp.377-383
• /
• 1997

To investigate the circadian variation in the bromobenzene metabolism, bromobenzene(400 mg/kg body weight) was intraperitoneally administered to the rats every other day for 6 days both in the night; 24:00 and the day; 12:00. Each group of animals was sacrificed at 8hr after last injection of bromobenzene. The contents of hepatic CYP were more increased in control rats of night phase than those of day phase but in case of bromobenzene treatment there were no differences in hepatic CYP between rats of the night phase and those of day phase and the injection of prednisolon inhibited the hepatic CYP content in rats. Furthermore, the decreasing rate of hepatic glutathione contents to the control was higher in rats of day phase than those of night phase by the bromobenzene treatment. And the hepatic glutathione S-transferase activities were increased both in control and bromobenzene treated rats of the night phase than those of day phase. On the other hand, liver weight per body weight(%), hepatic lipid peroxide content, serum levels of alanine aminotransferase were more increased both in bromobenzene-treated and control rats of the night phase than those in the day phase. These results indicate that the rats of night phase may induce more accelerated formation of bromobenzene 3,4-oxide from bromobezene than those of day phase in rats.

• Korean Journal of Community Nutrition
• /
• v.9 no.4
• /
• pp.536-544
• /
• 2004

To evaluate the antioxidative effect of maengjong-juk (Phyllostachys pubescens) extract coated rice in vivo system, maengjong-juk extract coated rice diets were fed to C57BL/6 mice for 16 weeks. Plasma total antioxidative capacity, hepatic lipid peroxidation, protein oxidation, activities of antioxidative enzymes and total glutathione content were measured. Plasma total antioxidative capacity was elevated significantly in maengjong-juk extract diets supplemented group in a dose dependant manner. Hepatic TBARS contents were significantly decreased in maengjong-juk extract diets supplemented group compared to high cholesterol group. Maengjong-juk extract coated rice diets suppressed the protein oxidation significantly in liver. Activities of hepatic antioxidative enzymes such as total SOD, CuㆍZn-SOD, Mn-SOD, GSH-Px and catalase activities of maengjong-juk extract coated rice diets were significantly higher than those of high cholesterol diet. Total hepatic glutathione content was significantly increased by maengjong-juk extract coated rice diets administration. According to this study, numerous antioxidative materials and phytochemicals containing in maengjong-juk extracts appear to protect antioxidative systems in C57BL/6 mice fed bamboo extract coated rice diet. (Korean J Community Nutrition 9(4): 536∼544, 2004)

• Food Science of Animal Resources
• /
• v.35 no.4
• /
• pp.479-485
• /
• 2015

This experiment investigated whether yogurt containing fermented pepper juice (FPJY) affects cholesterol level in high fat and high cholesterol diet (HFCD) fed rat. Twenty five Sprague-Dawley male rats of 7 wk were divided into 5 groups, and fed following diets for 9 wk; CON (control diet), HFCD (HFCD), PY (HFCD supplemented with 2% of plain yogurt), LFY (HFCD supplemented with 2% of FPJY), and HFY (HFCD supplemented with 5% of FPJY). In the LFY group, hepatic total lipid level decreased significantly compared to the HFCD group (p<0.05). Serum HDL cholesterol level tended to increase and hepatic total cholesterol level decreased and were comparable to the CON group (p>0.05). In HFY group, body weight and hepatic total lipid level significantly decreased over the HFCD group (p<0.05). Serum and hepatic total cholesterol level, kidney, and body fat weights decreased, and were compared to the CON group (p>0.05). Liver weight decreased as FPJY content was increased. Results suggested FPJY would inhibit organ hypertrophy and accumulation of body fat, hepatic lipid, and cholesterol in HFCD fed rat.

• Preventive Nutrition and Food Science
• /
• v.16 no.1
• /
• pp.1-7
• /
• 2011

The purpose of this study was to investigate the effect of enzyme-treated radish (Raphanus sativus L.) leaves on lipid metabolism in rats fed a high fat diet. Rats were divided into four experimental groups which were composed of a high fat diet group (HF group), a high fat diet with 10% radish leaf powder-supplemented group (MA group), a high fat diet with 5% enzyme-treated radish leaf powder-supplemented group (MB group) and a high fat diet with 10% enzyme-treated radish leaf powder-supplemented group (MC group). Total dietary fiber content of enzyme-treated radish leaves were greater than untreated radish leaves. Body weight gain and food efficiency ratio (FER) of the HF group increased compared to the MA, MB and MC groups. The serum total cholesterol, LDL-cholesterol and atherogenic index contents in the radish leaf powder-supplemented groups were lower than that of the HF group, while those values for the MB and MC groups were significantly lower than that of the HF group. The serum HDL-cholesterol contents of the MB and MC groups increased compared to the HF group. The hepatic triglyceride contents of the MA, MB and MC groups decreased compared to the HF group. In fact, the hepatic triglyceride contents of the MB and MC groups were significantly lower than the MA group. The hepatic total cholesterol contents of the MB and MC groups significantly decreased compared to those of the HF group. The fecal total cholesterol contents of the MA, MB and MC groups significantly increased compared to those of the HF group. These results indicate that supplementation with enzyme-treated radish leaves increase the useful fiber contents. Furthermore, it may have a pronounced impact on lipid metabolism of serum and liver in rats fed a high fat diet.

• Asian-Australasian Journal of Animal Sciences
• /
• v.24 no.8
• /
• pp.1148-1156
• /
• 2011

Compensatory growth of juvenile olive flounder Paralichthys olivaceus fed different diets with different feeding regime was compared. Four hundred fifty fish (twenty five fish per tank) were randomly distributed into 18 of 180 L flow-through tanks. Six treatments were prepared: fish were hand-fed with the control (C) diet to satiation twice a day, six days a week, for 8 weeks (C-8W treatment); and other groups of fish were starved for 2 weeks and then fed with the C, high protein (HP), high carbohydrate (HC), high lipid (HL), and combined protein, carbohydrate and lipid (CPCL) diets to satiation twice a day, six days a week, for 6 weeks, referred to as C-6W, HP-6W, HC-6W, HL-6W, and CPCL-6W treatments, respectively. Final body weight of fish in HP-6W treatment was higher than that of fish in C-6W, but not different from that of fish in C-8W, HC-6W, HL-6W and CPCL-6W treatments. Specific growth rate of fish in HP-6W treatment was higher than that of fish in all other treatments except for fish in CPCL-6W treatment. Feeding rate of fish in C-8W treatment was higher than that of fish in HP-6W, HC-6W, HL-6W and CPCL-6W treatments, but not different from that of fish in C-6W treatment. In addition, feeding rate of fish in C-6W treatment was higher than that of fish in HP-6W, HL-6W and CPCL-6W treatments. Feed and protein efficiency ratios of fish in HP-6W, HC-6W, HL-6W and CPCL-6W treatments were higher than those of fish in C-6W treatment. None of moisture, crude protein and ash content of the whole body of fish excluding the liver was different among treatments. Dietary supplementation of protein, carbohydrate, lipid and their combination could improve compensatory growth of fish when fish were fed for 6 weeks after 2-week feed deprivation; especially, supplementation of dietary protein was the most effective to improve compensatory growth of fish.

• Mycobiology
• /
• v.39 no.4
• /
• pp.283-289
• /
• 2011

The wild edible mushroom, Lentinus lepideus has recently been cultivated for commercial use in Korea. While the mushroom has been widely used for nutritional and medicinal purposes, the possible anti-hyperlipidemic action is unclear. The effects of dietary L. lepideus on plasma and feces biochemical and on the liver histological status were investigated in hypercholesterolemic rats. Six-wk-old female Sprague-Dawley albino rats were divided into three groups of 10 rats each. Biochemical and histological examinations were performed. A diet containing 5% L. lepideus fruiting bodies reduced plasma total cholesterol, triglyceride, low-density lipoprotein, total lipid, phospholipids, and the ratio of low-density to high-density lipoprotein. Body weight was reduced. The diet did not adversely affect plasma biochemical and enzyme profiles. L. lepideus reduced significantly plasma ${\beta}$- and pre-${\beta}$-lipoprotein, while ${\alpha}$-lipoprotein content was increased. A histological study of hepatic cells by conventional hematoxylin-eosin and oil red O staining revealed normal findings for mushroom-fed hypercholesterolemic rats. The present study suggests that a diet supplemented with L. lepideus can provide health benefits by acting on the atherogenic lipid profile in hypercholesterolemic rats.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.32 no.6
• /
• pp.882-886
• /
• 2003

Effect of kochujang (Korean red pepper soybean paste) on the reduction of body weight and lipid levels of adipose tissues and serum were investigated in the rats fed a high-fat diet. Male Sprague-Dawley rats were fed for four weeks with diets containing either a normal diet (ND, based on the AIN-76TM diet), high fat diet (HFD, supplemented with 20% corn oil in ND), CK diet (10% commercial kochujang in HFD), TK I diet (10% 0 day (non-) fermented traditional kochujang in HFD), TKII diet (10% 6-month fermented traditional kochujang in HFD), or RPP diet (3% red pepper powder in HFD). CK, TK I and TKII in the diet significantly reduced the body weight gain compared to the control (HFD), whereas red pepper powder had no such effect. TKII supplementation in the high-fat diet reduced epididymal and perirenal fat pad weight significantly (p<0.05). Liver total lipid was lower in TKII diet group than other kochujang and RPP diet groups. TKII and CK decreased the content of triglyceride in epididymal and perirenal fat pad that increased by high-fat diet. The cholesterol content of the perirenal fat pad was the lowest in the TKII diet group. TKII was also effective in lowering serum cholesterol level. These results indicated that properly ripened (fermented for 6-month) traditional kochujang exhibited more suppressive effects on body fat gain and lipid levels of adipose tissue and serum than RPP, commercial kochujang and the kochujang without fermentation.

• The Korea Journal of Herbology
• /
• v.31 no.2
• /
• pp.13-19
• /
• 2016

Objectives : The present study was designed to evaluate the protective effect of Allium tuberosum (AT) extract on atherosclerosis in LDL receptor knockout (LDLr KO) mouse fed western diet.Methods : The AT was extracted 70% ethanol. The experimental groups were divided with four groups of LDLr KO mice, one group fed a normal diet and the others fed a Western diets for 8weeks. Two Western diet groups were orally administered AT extract at dosage of 100 and 300 mg/kg body weight. The body weight and food intake were measured every day. We measured levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and Glucose in serum. Also, effect of AT extract performed using H&E staining.Results : The AT treatment groups showed decrease in body weight and food efficiency in comparison with control group. Blood biochemistry parameters such as TG, TC, LDL, and glucose levels were increased in control group, while AT treatment groups were reduced. Also, the increased levels of ALT and AST were improved by AT extract. We confirmed that the weights of liver, kidney, subcutaneous fat, epididymal fat, kidney leaf fat, and intraabdominal fat were change in LDLr KO mice treated AT extract. In addition, histopathological changes in liver and aorta were similar to normal group.Conclusions : Based on these results, the AT extract is considered to make prevention of atherosclerosis through reduction and functional improvement of the liver and vascular endothelial cells in the body fat accumulation and lipid content in LDLr ko mouse model.

• Journal of Life Science
• /
• v.22 no.1
• /
• pp.1-6
• /
• 2012

The present study examined the effects of consumption of Jeju ground water containing vanadium components on oxidative stress in obese (ob/ob) mice. Intake of Jeju ground water decreased the generation of oxidative stress induced-lipid peroxidation in the liver of ob/ob mice It also enhanced the enzymatic antioxidant defense system by increasing the protein expression and activity of superoxide dismutase, catalase, and glutathione peroxidase in liver tissues. Jeju ground water intake also upregulated the intracellular content of reduced glutathione. The induction of antioxidant enzyme expression by consumption of Jeju ground water was mediated by the erythroid transcription factor NF-E2 (Nrf2). Increased nuclear expression of phospho Nrf2 was observed in ob/ob mouse liver cells following ntake of Jeju ground water. These results suggest that consumption of Jeju ground water stimulated the antioxidant defense system in the livers of ob/ob mice via induction of Nrf2.