• 환경생물
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• 제25권4호
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• pp.289-296
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• 2007

본 연구에서는 내분비계 장애물질으로 알려져 있고, 플라스틱 제품의 가소제로 사용되고 있는 di-(2-ethylhexyl) phthalate (DEHP)가 흰쥐의 간세포 미세구조와 간조직내 metallothionein (MT)의 발현 양상에 미치는 영향을 조사하였다. DEHP는 흰쥐 간세포의 미세구조와 MT 발현에 영향을 주었다. 실험군의 경우 조면소포체가 발달하고, 미토콘드리아가 증가하며 리소솜 혹은 퍼옥시좀들이 증가하는 경향을 나타냈다. 한편, MT 발현의 변화를 면역세포 화학적 방법과 western Blot을 수행한 결과 저농도 투여군에서는 약 1.5배, 고농도 투여군에서는 약 2배 가량 증가하는 결과를 나타냈다. 결론적으로 DEHP는 흰쥐 간세포의 구조와 기능에 영향을 주는 것으로 판단되며, 이러한 세포내 스트레스가 MT 발현 증가 현상과 연관이 있을 것으로 판단된다.

• 한국식품영양과학회지
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• 제18권4호
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• pp.444-448
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• 1989

세포증식에 관한 개체발생의 일주리듬을 검토하기 위하여 부화직후의 병아리 간장 DNA 합성 리듬 및 간 세포증식의 개체발생에 대해서 검토한 결과 포유류에 있어서 DNA 합성 리듬은 이유기 후기에 출현하는데 비하여 조류(병아리)에 있어서 DNA합성 리듬은 부화 12시간후 바로 출현하였고 간장의 DNA 합성 리듬은 사료섭취에 의해 리듬주기가 유지되었다. 한편 DNA 합성 활성은 절식에 의해 감소되었으며 DNA 합성의 리듬변화는 사료섭취에 의해서도 변화하였지만 빛의 조건에 의해서도 많은 영향을 받았다. 이러한 간 DNA 합성은 12시간 늦은 위상에서 유사분열도 동반하였다.

• BMB Reports
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• 제44권4호
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• pp.238-243
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• 2011

We generated 16,993 expressed sequence tags (ESTs) from two libraries containing full-length cDNAs from the brain and liver of the Korean Jindo dog. An additional 365,909 ESTs from other dog breeds were identified from the NCBI dbEST database, and all ESTs were clustered into 28,514 consensus sequences using StackPack. We selected the 7,305 consensus sequences that could be assembled from at least five ESTs and estimated that 12,533 high-quality single nucleotide polymorphisms (SNPs) were present in 97,835 putative SNPs from the 7,305 consensus sequences. We identified 58 Jindo dog-specific SNPs in comparison to other breeds and predicted seven synonymous SNPs and ten non-synonymous SNPs. Using PolyPhen, a program that predicts changes in protein structure and potential effects on protein function caused by amino acid substitutions, three of the non-synonymous SNPs were predicted to result in changes in protein function for proteins expressed by three different genes (TUSC3, ITIH2, and NAT2).

• 대한약침학회지
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• 제5권1호
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• pp.81-90
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• 2002

This dissertation was designed to evaluate the effect of BCL(refinded Bambusae Caulis in Liqua-men) oral administration and herbal acupuncture on alcohol metabolism and liver function. For this study. mice were damaged by a large quantity of alcohol and received treatment of either BCL 1 mg/kg in oral or BCL 250㎍/kg in herbal acupuncture-BL18 . BL19 bilateral. and then such parameters as GOT. GPT. catalase and superoxide dismustase(CuZn-SOD, Mn-SOD) were measured. The results of the experiments were summarized as follows. 1. Compared with control group, the proper degree of alcohol in serum was not significantly differ from oral administration group and herbal acupuncture group. 2. Compared with control group. the activity of GOT in serum was significantly reduced both oral administration and herbal acupuncture group. 3. Compared with control group. the activity of GPT in serum was significantly reduced both oral administration and herbal acupuncture group. 4. The activity of catalase in liver cell tissue, compared with control group. was not sigificantly affected either by oral administration and herbal acupuncture group. 5. The activity of CuZn-SOD in liver cell tissue was not significantly change in herbal acupuncture and oral administration group. The activity of Mn-SOD was significantly increased in oral administration group. while it was not the case in acupuncture group. In conclusion. we consider that BCL oral administration and herbal acupuncture is highly effetive in recovering alcohol metabolism and liver disfunction induced by alcohol.

• 대한수의학회지
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• 제36권3호
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• pp.699-707
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• 1996

This study was designed to examine the involvement of lead in function of target organ, and the protective effect of selenium in lead-treated guinea pigs for 8 weeks. The effects of exposure to 0.5% lead acetate(lead) and/or 4ppm sodium selenite(selenium) in feed on serveral aspects were evaluated by measuring thyroid stimulating hormone(TSH), triiodothyronine($T_3$), thyroxine($T_4$), serum biochemical activities, organ weights, and serum and organ lead concentrations in growing animals. The many indicators of endocrine function(TSH, $T_3$, and $T_4$ in serum), enzyme and biochemical activities(${\alpha}$-glutamyltranspeptidase, alkaline phosphatase, lactate dehydrogenase, triglyceride, creatinine, $Ca^{2+}$ in serum), and organ weights(kidney, spleen and testis) were correlated with lead exposure or showed significantly different mean values between the exposed and controls. These changes on some aspects were reversed by combination-fed of selenium, but did not statistically significant. The organ(kidney, liver, spleen, testis and brain) and serum lead concentrations of lead-fed group were clearly higher than that of controls. Selenium supplementation resulted in a significant protection against lead accumulation in liver and testis. These results suggest that lead can cause a toxic effect on several organ and that selenium seems to has a protective effect on specific reaction by lead-induced organic function toxicity.

• 사상체질의학회지
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• 제15권3호
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• pp.62-73
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• 2003

Lee, Jema recognized human being as human figure and human nature according to 'if threre is a thing, there are a law to explain the thing'. And he thought Sa-Sang is the most basic and proper concept to explain things and changes. This paper is to find his medical view why human beings are classified into four types. 1. A medical clue to classify human being is different viscera function. 2. The size of viscera means the strength and weakness of metabolic function of viscera. 3. The essence of different viscera function is related to metabolism of water and food, air and fluid. Lungs and Liver do metabolic function through exhale and inhale of air and fluid. Spleen and Kidneys do metabolic function through intake an d output water and food. 4. It is most important to consider and to treat the condition of small size viscera.

• Toxicological Research
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• 제34권3호
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• pp.221-229
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• 2018

Tenofovir nanoparticles are novel therapeutic intervention in human immunodeficiency virus (HIV) infection reaching the virus in their sanctuary sites. However, there has been no systemic toxicity testing of this formulation despite global concerns on the safety of nano drugs. Therefore, this study was designed to investigate the toxicity of Tenofovir nanoparticle (NTDF) on the liver and kidney using an animal model. Fifteen adult male Sprague-Dawley (SD) rats maintained at the animal house of the biomedical resources unit of the University of KwaZulu-Natal were weighed and divided into three groups. Control animals (A) were administered with normal saline (NS). The therapeutic doses of Tenofovir (TDF) and nanoparticles of Tenofovir (NTDF) were administered to group B and C and observed for signs of stress for four weeks after which animals were weighed and sacrificed. Liver and kidney were removed and fixed in formal saline, processed and stained using H/E, PAS and MT stains for light microscopy. Serum was obtained for renal function test (RFT) and liver function test (LFT). Cellular measurements and capturing were done using ImageJ and Leica software 2.0. Data were analysed using graph pad 6, p values < 0.05 were significant. We observed no signs of behavioural toxicity and no mortality during this study, however, in the kidneys, we reported mild morphological perturbations widening of Bowman's space, and vacuolations in glomerulus and tubules of TDF and NTDF animals. Also, there was a significant elevation of glycogen deposition in NTDF and TDF animals when compared with control. In the liver, there were mild histological changes with widening of sinusoidal spaces, vacuolations in hepatocytes and elevation of glycogen deposition in TDF and NTDF administered animals. In addition to this, there were no significant differences in stereological measurements and cell count, LFT, RFT, weight changes and organo-somatic index between treatment groups and control. In conclusion, NTDF and TDF in therapeutic doses can lead to mild hepatic and renal histological damage. Further studies are needed to understand the precise genetic mechanism.

• 한국멀티미디어학회논문지
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• 제25권5호
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• pp.757-768
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• 2022

In this paper, we proposed a system that visualizes a hologram device in 3D by utilizing the CT image segmentation function based on artificial intelligence deep learning. The input axial CT medical image is converted into Sagittal and Coronal, and the input image and the converted image are divided into 3D volumes using ResUNet, a deep learning model. In addition, the volume is created by segmenting the tumor region in the segmented liver image. Each result is integrated into one 3D volume, displayed in a medical image viewer, and converted into a video. When the converted video is transmitted to the hologram device and output from the device, a 3D image with a sense of space can be checked. As for the performance of the deep learning model, in Axial, the basic input image, DSC showed 95.0% performance in liver region segmentation and 67.5% in liver tumor region segmentation. If the system is applied to a real-world care environment, additional physical contact is not required, making it safer for patients to explain changes before and after surgery more easily. In addition, it will provide medical staff with information on liver and liver tumors necessary for treatment or surgery in a three-dimensional manner, and help patients manage them after surgery by comparing and observing the liver before and after liver resection.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2000년도 제28회 학술심포지엄
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• pp.9-10
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• 2000

The aging process is multifactorial and results from the combined effects of inherited(genetic) and acquired factors including life style, food habits, physical activity, and diseases. That give rise to the various approaches in aging. We are trying to study biological changes with aging, In detail we are focused on gene and protein function accompanied by normal or abnormal aging process, especially our efforts are aimed at revealing the functional relationship of proteins in aging as a final product of gene. We expect that proteomic approach to the study of protein function involved in aging should give us variety of integrated data to understand biological changes of long lived lives, We have applied expression proteomics to rat liver bred in dietary restriction or in at libitum to elucidate the effects of food habit on aging. Expression proteomics shows us protein profile in a selected tissue or cells as a whole and gives us the information about protein expression level, posttranslational modification and degenerative modification of expressed proteins. Comparative analysis of young and old rat liver by two dimensional gels shows that gene expression of several proteins was down regulated in old rats and some protein expression level is increased with aging. Dietary restriction slows down these changes of gene expression and in some proteins there's no difference in protein expression level at same ages in comparison with rats bred in at libitum. About forty protein was identified by peptide mass fingerprint with MALDI-TOF and rest of the protein of interest is in the course of identification, Also we are trying to make mitochondrial and cytosolic proteom reference map. These suborganelle proteom map will gives us the information about low abundance proteins and cellular localization of proteins. Proteomics is a growing methodology to study biological system. High throughput qualitative and qualitative aspect of this approach will gives us large amount of integrated information and speed up our understanding about biological system

• 동의생리병리학회지
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• 제21권4호
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• pp.822-834
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• 2007

This study searched the philosophical and medical thought of mutual understanding and human body. Mutual understanding is a fundamental problem in all branches of oriental studies. In other words, mutual understanding becomes the existential foundation of heaven-earth-human(三才). So human beings, heaven and the earth can't exist if there is no mutual understanding. It comes out the problem of self-consciousness in philosophy, and qi movement pattern like upward, downward, inward and outward movement in traditional korean medicine. Human beings have mutual understandings with heaven and the earth from a macroscopic standpoint, on the other side the human body from a microscopic standpoint. Qi movement is the mutual understanding and response of qi in human body, so with which the physiological functions and pathologic changes of viscera and bowels comes out. Therefore we want to present how to complete qi movement between viscera-bowels and organs to examine mutual understandings in human body closely. The results was summarized as follows; First, upbearing the clear yang and down bearing the turbid yin of spleen-stomach is main pivot of upward and downward of qi movement, and it is true form of mutual understandings between viscera and bowels, so upward, downward, inward and outward movement of whole viscera and bowels can be controlled by spleen-stomach. Second, by restraining relationships between downward heart fire and upward kidney water, heart fire and kidney water have close communications physiologically and pathologically as upper-lower, yin-yang and water-fire. Third, by restraining relationships between upbearing and effusion of liver and purification and down-sending of lung, liver and lung are outer circles of upward and downward movement, so they have antagonistic functions. Firth, by the relationships between upbearing the clear and down bearing the turbid of spleen-stomach and free coursing of liver, free coursing of liver-gallbladder is the essential requirement that it certify transportation and transformation of spleen-earth, so the disease of liver-gallbladder induce spleen-stomach disease pattern to affect its function of transportation and transformation. Fifth, by spleen and kidney, spleen yang is based on kidney yang, so the weakness of kidney yang can affect the function of transportation and transformation of spleen-stomach as it can't warm spleen earth. Sixth, by homogeny of liver-kidney, essence and blood of liver-kidney and ministerial fire of liver-kidney have mutal generation and limitation physiologically and they mutually are affected in pathologically.