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Ultrastructure and Metallothionein Expression in Rat Liver Treated with Di-(2-ethylhexyl)phthalate  

Kim, Da-Ham (Department of Biology, Soonchunhyang University)
Moon, Seung-Hoon (Department of Biology, Soonchunhyang University)
Lee, Mi-Young (Department of Medical Biotechnology, Soonchunhyang University)
Lee, Jong-Hwa (Department of Environmental Health Science, Soonchunhyang University)
Park, Young-Hyun (Department of Food Science and Nutrition, Soonchunhyang University)
Shin, Kil-Sang (Department of Biology, Soonchunhyang University)
Kim, Wan-Jong (Department of Biology, Soonchunhyang University)
Publication Information
Korean Journal of Environmental Biology / v.25, no.4, 2007 , pp. 289-296 More about this Journal
Abstract
Di-(ethyhexyl) phthalate (DEHP), commonly used as a plasticizer for manufacturing flexible vinyl products, has been the topic of extensive research, especially concerning endocrine disrupting properties. Metallothionein (MT) is a low molecular weight (6,000$\sim$7,000 Da), cysteine-rich (22$\sim$23%), metal-binding protein and is known to be induced by extrinsic factors such as chemical agents and stresses. Some of the known function of MT include detoxification of heavy metals and alkylating agents and neutralization of free radicals. Nonetheless, the definitive physiological function of MT are still unknown. This study was carried out to investigate the effects of DEHP on the ultrastructural changes and the expression of MT of the rat liver. The rats were orally intubated with either corn oil (experimental control) or 0.5 mg, 1.5 mg and 4.5 mg DEHP kg$^{-1}$ day$^{-1}$ in 0.5 mL of corn oil for 15 days before sacrificing and sampling. DEHP induced mild ultrastrctural changes of some cell organelles such as rough endoplasmic reticulum, mitochondria, lysosomes and peroxisomes in the rat liver treated with DEHP. In the respect of immunogold labelling and Western blotting, MT expression of the liver tissue was up-regulated by DEHP. In conclusion, DEHP has effects on the ultrastructures and hepatic function for MT expression in rat.
Keywords
Di-(ethylhexyl)phthalate (DEHP); liver; metallothionein;
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