• Journal of the Optical Society of Korea
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• 제19권3호
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• pp.228-236
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• 2015

Fluorescence recovery after photobleaching (FRAP) is a well-established method commonly used to measure the diffusion of fluorescent solutes and biomolecules in living cells or tissues. Here a fiber-optic-based FRAP (f-FRAP) system was developed, and validated using macromolecules in water and agarose gels of different concentrations. We applied f-FRAP to measure the site-specific diffusion of fluorescein (NaFluo) in peritoneal membranes (PMs) on the liver, cecum, and kidney of a living rat during peritoneal dialysis. Diffusion of fluorescein in PM varied in a time-dependent manner according to the type of organ ($D_{PM\;on\;Liver}/D_{NaFluo}=0.199{\pm}0.085$, $D_{PM\;on\;Cecum}/D_{NaFluo}=0.292{\pm}0.151$, $D_{PM\;on\;Kidney}/D_{NaFluo}=0.218{\pm}0.110$). The proposed method allows direct quantitative measurement of the three-dimensional diffusion in local PM in vivo, which was previously inaccessible by peritoneal function test methods such as peritoneal equilibration test (PET) and standardized PM assessment (SPA). f-FRAP is promising for local and dynamic assessments of peritoneal pathophysiology and the mass transport properties of PMs, presumed to be affected by variation of tissue structures over different organs and functional changes of the PM with years of peritoneal dialysis.

• 대한의생명과학회지
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• 제18권3호
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• pp.237-243
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• 2012

This study was undertaken to know the effect of fat diet (for eight weeks) on changes of inflammatory markers [tumor necrosis factor (TNF-${\alpha}$) and prostaglandin $E_2$ ($PGE_2$)] and blood coagulation system [platelet aggregation function (PAF), prothrombin time (PT), activated partial thromboplastin time (aPTT)] in rats. Serum TNF-${\alpha}$, $PGE_2$, biochemical markers, PAF, PT, aPTT, and body weight were measured and compared between the control (normal diet-rats) and the fat group (fat diet-rats). The weights in the fat group were higher than those of the control group. TNF-${\alpha}$, $PGE_2$, glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and creatinine levels were greater in the fat group compared with the control group. The degree of platelet aggregation was lower, whereas PT and aPTT levels were longer in the fat group than in the control group. These findings have shown that fat diet may cause inflammatory response, diabetes, liver and renal dysfunction, and disturbances of fibrinolysis and coagulation system.

• Asian-Australasian Journal of Animal Sciences
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• 제7권4호
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• pp.471-474
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• 1994

Five experimental groups with five adult male rats in each, were exposed to 20, 35, 40 and $45^{\circ}C$ air temperature for 50-70 minutes, and to $50^{\circ}C$ for 30-50 minutes, respectively. Food and drinking water were not permitted during the exposure. Blood samples were obtained by heart puncture immediately after the thermal treatment. All the rats were hyperthermic (p<0.01) as compared to the controls ($20^{\circ}C$). Hyperthermia was associated with hypoglycemia which was significant (p<0.01) at 45 and $50^{\circ}C$ exposures. Plasma levels of GOT and GPT declined at 35 and $40^{\circ}C$ reaching the lowest (p<0.05) level at $45^{\circ}C$, while at $50^{\circ}C$ GOT level was elevated by 45% but GPT was normal as compared to the controls. Differences between groups were significant (p<0.01) for GOT and insignificant for GPT. Hematocrit value increased significantly (p<0.01) at 45 and $50^{\circ}C$, indicating hemoconcentration. It could be concluded that severe heat stress (45 and $50^{\circ}C$) resulted in critical hyperthermia, hypoglycemia, disturbed liver function, body dehydration, and hemoconcentration leading to death.

• 대한한의학회지
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• 제35권2호
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• pp.69-78
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• 2014

Objectives: This case report was executed to confirm the efficacy and safety of Injincheonggan-tang on two patients with HBeAg-negative chronic hepatitis B. Methods: We reviewed the changes of clinical progress and laboratory records of patients with chronic hepatitis B who did not want to take any antiviral or interferon therapy. One patient has visited the department of internal medicine I of Kyung Hee University Korean Medicine Hospital, from October 2010 and the other, from October 2006. Both of them were prescribed with Injincheonggan-tang and laboratory tests were conducted as well. Results and Conclusions: AST, ALT and HBV DNA which are significant indicators of liver function were controlled in the normal range during the herbal treatment period and subjective clinical symptoms were also improved after taking Injincheonggan-tang.

• Food Science and Biotechnology
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• 제17권6호
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• pp.1261-1264
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• 2008

In the present study, a randomized, double-blind, placebo-controlled trial to determine the effect of conjugated linoleic acid (CLA) supplementation (50:50 ratio of cis-9, trans-11 and trans-10, cis-12 isomers) for 8 weeks on body composition and biochemical parameters in healthy overweight/obese (body mass index, BMI${\geq}23\;kg/m^2$) Korean subjects was performed, Thirty participants (3 males and 27 females) were randomized to receive placebo (2.4 g olive oil/day) or 2.4g/day CLA (mixture containing 36.9% of cis-9, trans-11 and 37.9% of trans-10, cis-12). Eight weeks of CLA supplementation significantly decreased body weight by -0.75kg, BMI by $-0.27\;kg/m^2$, and hip circumference by -1.11 cm. The reduction of body weight was ascribed to the reduction of body fat mass (-0.59 kg) and lean body mass (-0.18 kg), although these changes were not significant. No significant differences in serum lipid profiles, liver function enzyme activities, and protein concentration were observed in either the CLA or placebo groups. These results indicate that short tenn supplementation (8 weeks) with CLA (2.4 g/day) may decrease body weight in Korean overweight/obese subjects.

• 대한한방내과학회지
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• 제44권4호
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• pp.803-813
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• 2023

Objective: This case report outlines an investigation into the efficacy of Korean medicine treatment in a patient who presented with acute cholangitis accompanying common bile duct stones. The patient underwent percutaneous transhepatic gallbladder drainage and subsequently experienced resolution of the common bile duct stones. Methods: The patient was treated with Shihosogan-tang, general acupuncture, ear acupuncture, moxibustion, and cupping therapy. Changes in symptoms were evaluated using a self-reported numerical rating scale (NRS) score and a visual analog scale (VAS) score each morning. Laboratory tests were conducted to examine serum amylase, serum lipase, and liver function. Results: After 29 days, the NRS scores for indigestion and fatigue and the VAS score for abdominal pain all decreased. Additionally, the laboratory test results showed improvement. Discussion: The results suggest that Korean medicine could be effective in treating symptoms of acute cholangitis. However, further research is necessary.

• 대한수의학회지
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• 제37권4호
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• pp.883-897
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• 1997

Present study was undertaken in order to find out the most significant laboratory test for the early diagnosis of ethylene glycol poisoning in dogs. Seven dogs weighing from 5.0kg to 19.3kg were administered orally 6ml or l0ml of ethylene glycol per kilogram of body weight. In addition to clinical observations, blood samples were taken from the jugular vein on 0, 1, 3, 6, 12, 24 and 48 hours or until death after the adminisration and examined for the hematological, blood chemical, venous blood gas and electrolyte changes. Urine samples were simultaneously taken and examined for pH, protein, glucose and calcium oxalate crystals in the sediments. The results were as follows : 1. All dogs showed marked hypothermia in addition to the characteristic signs of ethylene glycol poisoning until death. 2. No significant hematological changes were observable after the administration of ethylene glycol except the secondary dehydration and stress leukogram. 3. Chemical values related to the liver function(serum glutamic pyruvic transaminase, total serum protein, serum albumin, serum total bilirubin) showed no significant changes during the experimental period in all dogs. 4. Chemical values related to the renal function(BUN and creatinine) showed significant(p<0.0l, p<0.001) increase from 24 hours to death in all dogs. 5. All dogs showed significant(p<0.01, p<0.001) decrease in blood pH from 3 hours to death after the ethylene glycol administration. 6. All dogs showed significant(p<0.05, p<0.01, p<0.001) increaes in anion gap from 1 hour to death after the ethylene glycol administration. 7. All dogs showed significant(p<0.05, p<0.01) decrease in urine pH from 1 hour to 6 hours after the administration of ethylene glycol. 8. Characteristic envelope form of calcium oxalate crystals were found in the urinary sediment from 6 hours after the administration of ethylene glycol in all dogs. 9. Histologically calcium oxalate crystals were found in the renal rubles in all dogs. 10. From these results, it may be concluded that the examinations of urinary sediments for the calcium oxalate crystals, blood or urinary pH and blood anion gap are most reliable tests for the early diagnosis of ethylene glycol poisoning in dogs.

• 생명과학회지
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• 제31권3호
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• pp.346-355
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• 2021

이 연구의 목적은 Bacillus polyfermentricus KJS-2 (BP2)와 Bacillus mojavensis KJS-3 (Moja3) 및 이들 균주 복합물의 투여가 혈청 지질 수준을 조절할 수 있는지에 대한 가능성을 밝히기 위한 것이다. 고지질혈증 유발 설치류를 대상으로 균주를 2주 동안 경구 투여한 후 혈액 내 세포수준의 변화, 대사기능 평가, 혈중 지질 수준의 변화를 관찰하였다. 백혈구내 주요세포변화를 측정한 결과 균주 투여에 따른 혈중 백혈구(WBC) 수준에는 크게 영향을 미치지는 않았다. 단위 혈액 내 혈소판(PLT) 수치는 Triton WR-1339처리시 18.4% 감소하였고 약물투여그룹 및 균주 투여그룹에서 다시 정상 그룹 수준으로 회복되었다(p<0.05). Triton WR-1339로 유도된 고지혈증에 의해 적혈구의 기능적인 변화는 초래하지 않았다. 혈중 AST, ALT, BUN, Creatinine 수치는 혼합 균주투여 그룹에서 모두 회복하여 간기능, 신장기능에 영향을 미치지 않았다. 혈중 지질수준 변화는 미생물 균주 투여그룹에서 트리글리세라이드(TG)와 총콜레스테롤(TC) 혈청 수치가 감소했고, 혈청 HDL 콜레스테롤 수치는 증가했다. BP2가 생산하는 SMA의 HMG-CoA reductase 저해율은 atorvastatin에 비해 1,000배 낮은 농도에서 비슷한 활성을 보였다. 또한, 고지혈증 유발 설치류에 미생물 균주의 복합투여는 식이에 영향을 미치지 않으면서 체중증가율은 감소시켰다. 따라서 BP2와 Moja3 균주 복합투여시 혈청 지질 수치를 조절함으로써 혈액 순환을 개선시킬 수 있을 것이다.

• 대한장애인치과학회지
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• 제9권1호
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• pp.30-35
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• 2013

The four principles of treatment of odontogenic infection are as follows : (1) removal of the cause, (2) establishment of drainage, (3) institution of antibiotic therapy, and (4) provision of supportive care, including proper rest and nutrition. A separate incision is required to establish drainage, especially in the case of extensive fascial space infections. There are four principle causes for active bleeding in the immediate incision & drainage phase; (1) vascular wall alteration (infection, scurvy, chemicals), (2) disorder of platelet function, (3) thrombocytopenic purpuras, (4) disorders of coagulation (liver disease, anticoagulation drug). If the hemorrhage from incision & drainage site is aggressive, the site must be packed with proper wet gauze and wound closure & drainage dressing are applied. The specific causes of bleeding may be associated with hypoxia, changes in the pH of blood & chemical changes affecting vascular contractility and blood clotting. This is a case report of bleeding control by the circumferential suture & drainage on active bleeding incision & drainage site of temporal space abscess due to advanced odontogenic infection in a multiple medically compromised disabled patient.

• 한방안이비인후피부과학회지
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• 제19권2호
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• pp.50-58
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• 2006

Background and Objectives : Although the incidence of allergic rhinitis has increased specific therapeutics with a certain medicine is not well developed yet. In therapeutics of allergic rhinitis, limited medicines just have been used in the clinin. Sochungyong-tang has been a widespread medicine for allergic rhinitis. However, okbyeongpung-san has been taken for raising immunity. In medical treatment of allergic rhinitis, raising immunity is a necessary part of the process. In this reason, this study aimed to find out therapeutic effects of combination of sochungyoung-tang and okbyeongpung-san extract on allergic rhinintis of nasal tissue in rats. Materials and Methods : The Sprague-Dawley male rats, about 200g of body weight, were divided into three groups: the normal group, the control groups, and the sample groups. With the Levine and Vaz method, the allergic reaction was induced with ovalbumin as antigen in the intraperitoneal route. Rats of the sample groups were treated with 2cc of sochungyoung-tang plus okbyeongpung-san extract once a day for 28days. I observed historical changes in nasal tissue. I also found changes in the segment of lympocyte and neutrophil of Leukocyte and erythrocyte. I used the statistical methods of post Hoc test(p<0.05) Results : The increase of the goblet cell and mucous secretion in the sample groups was rare when compared to the control groups. Effects of sochungyoung-tang plus okbyeongpung-san extract on the liver function were also studied in rats. Treatment of sochungyoung-tang plus okbyeongpung-san extract did not affect GOT and GPT. The number of lymphocyte was decreased, while the number of erythrocyte was increased in sample groups. Conclusion : This study shows that sochungyoung-tang plus okbyeongpung-san plus okbyeongpung-san extract has considerable effects on allergic rhinitis.