• Natural Product Sciences
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• 제26권2호
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• pp.171-175
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• 2020

Liriodendron tulipifera, belonging to the family Magnoliaceae, is commonly called tulip tree. Four N-acetylated aporphine alkaloids, N-acetylnornuciferine (1), N-acetylanonaine (2), N-acetyl-3-methoxynornuciferine (3), and N-acetyl-3-methoxynornantenine (4) were isolated from the roots of L. tulipifera. Although the purity of each compound (1 - 4) was determined to be 97, 96, 99, and 98%, respectively, the ¹H and ¹³C NMR spectroscopic data of the aporphine alkaloids 1 - 4 displayed all signals in duplicate, indicating the presence of two rotamers due to restricted rotation of N-COCH₃ functionality in solution status. The absolute configurations of 1 - 4 w ere established by measuring specific rotation and comparison with the reported data. This is the first report on the ¹H and ¹³C NMR assignments of N-acetyl-3-methoxynornuciferine (3) and N-acetyl-3-methoxynornantenine (4). This study provides advanced NMR spectroscopic data for the structure determination of rotameric aporphine alkaloids.

• Journal of the Korean Wood Science and Technology
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• 제52권2호
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• pp.101-117
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• 2024

The conversion characteristics of the major components of Liriodendron tulipifera were investigated during acid-catalyzed organosolv pretreatment. Glucan in L. tulipifera was slowly hydrolyzed, whereas xylan was rapidly hydrolyzed. Simultaneous hydrolysis and degradation of xylan and lignin occurred; however, after complete hydrolysis of xylan at higher temperatures, lignin remained and was not completely degraded or solubilized. These conversion characteristics influence the structural properties of glucan in L. tulipifera. Critical hydrolysis of the crystalline regions in glucan occurred along with rapid hydrolysis of the amorphous regions in xylan and lignin. Breakdown of internal lignin and xylan bonds, along with solubilization of lignin, causes destruction of the lignin-carbohydrate complex. Over a temperature of 160℃, the lignin that remained was coalesced, migrated, and re-deposited on the surface of pretreated solid residue, resulting in a drastic increase in the number and content of lignin droplets. From the results, the characteristic conversions of each constituent and the changes in the structural properties in L. tulipifera effectively improved enzymatic hydrolysis in the range of 140℃-150℃. Therefore, it can be concluded that significant changes in the biomass recalcitrance of L. tulipifera occurred during organosolv pretreatment.

• 대한본초학회지
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• 제30권4호
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• pp.1-9
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• 2015

Objectives : The present study was under taken to characterize chemical composition, antioxidant and cyto-protecting capacity of essential oil obtained from leaves of Liriodendron tulipifera L. Methods : Essential oil from the leafof L. tulipifera L. (EOLL) was extracted by hydro-distillation process and further its chemical composition was evaluated by GC-MS analysis. The in vitro antioxidant potential of the EOLL was determined by DPPH ^●, ABTS ^●+, superoxide and nitric oxide free radical scavenging activity using different concentrations in the range of 50-800 μg/mL. In addition, cyto-protecting property of the EOLLwas determined by MTT assay on Raw 264.7 macrophage cells challenged with hydrogen peroxide (H ₂ O ₂ ). Results : The result of GC-MS analysis showed presence of 34 volatile compounds, principally germacrene D, spathulenol, and α -cadinol in EOLL. The in vitro antioxidant assays of EOLL at the highest used concentration of 800 μg/mL showed 81.62, 84.29, 83.59 and 58.59% inhibition of DPPH ^●, ABTS ^●+, superoxide, and nitric oxide radicals, respectively. It also showed ferric reducing ability with 1310.04 mM Fe (II)/g of essential oil. The EOLL at three different concentrations (200, 400 and 800 μg/mL) protected the cells from H ₂ O ₂ -induced cell damage through scavenging intracellular ROS. Conclusion : The findings from the study suggest that essential oil isolated from leaves of L tulipifera L. is a potent sources of natural antioxidants, which could be used to treat the diseases associated with oxidative stress condition.

• 펄프종이기술
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• 제41권1호
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• pp.61-66
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• 2009

Effects of aqueous ammonia soaking treatments to yellow poplar (Liriodendron tulipifera L.) were investigated to focus on chemical compositional changes and enzymatic hydrolysis characteristics changes by this treatment. Treatment temperature and time were main variables. At 3 different levels of aqueous ammonia soaking temperature and time ($145^{\circ}C$ -1 h, $90^{\circ}C$ -16 h and $45^{\circ}C$ - 6 days), lower temperature and longer soaking time led to more xylan removal based on carbohydrate compositional analysis. However, at higher temperature treatment led to more enzymatic saccharification of cellulose to glucose by commercial cellulose mixtures (Celluclast 1.5L and Novozym 342 from Novozyme, Denmark). Cellulose hydrolysis was gradually increased with increasing enzymatic hydrolysis time but xylan hydrolysis was leveled out at early stage (less than 10 h) of enzymatic hydrolysis.

• Journal of the Korean Wood Science and Technology
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• 제32권6호
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• pp.43-49
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• 2004

Three flavonoids, quercetin, taxifolin, and kaempferol were isolated from the woods of Liriodendron tulipifera. Their structures were determined by spectral analysis. Based on 1, l-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity method, the antioxidative activities of three isolated compounds and their acetates were measured in order to search for natural antioxidants. The IC₅₀ of quercetin (1), taxifolin (2), and kaempferol (3) were 3.6, 3.9, and 4.1 ㎍/㎖, respectively.

• 한국산림과학회지
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• 제105권4호
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• pp.463-471
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• 2016

백합나무(Liriodendron tulipifera L.)의 자원량 파악 및 바이오매스 통계자료 구축을 위해 줄기밀도, 바이오매스 확장계수, 뿌리함량비를 구하였으며, 흉고직경과 수고를 이용한 상대생장식을 개발하였다. 이를 위해 지역과 경급을 고려하여 총 40본의 표본목을 벌채하였고 21본은 뿌리까지 굴취 하였다. 본 연구 결과에 의하면 백합나무의 줄기밀도는 $0.43g{\cdot}cm^{-3}$, 바이오매스 확장계수는 1.2, 뿌리함량비는 0.2이며, 각각의 불확실성은 3.9%, 4.6%, 24.1% 이었다. 백합나무 지상부 상대생장식은 $W=0.060D^{2.524}$이었고, 전체 바이오매스 및 지하부 상대생장식은 각각 $W=0.063D^{2.578}$, $W=0.010D^{2.591}$이었다.

• 약학회지
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• 제34권2호
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• pp.106-111
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• 1990

The isolation and identification of an antibacterial component, from the leaves of Liriodendron tulipifera. K. Kotch against a cariogenic bacterium Streptococcus mutans OMZ 176, were carried out for developing of anticariogenic agents. The bioactive component was elucidated as ${\beta}-liriodenolide$, which was isolated newly from the leaves of L. tulipifera. The minimal inhibitory concentration (MIC) of ${\beta}-liriodenolide$ was $100\;{\mu}g/ml$ and the antibacterial activity was stronger than that of berberine. ${\beta}-Liriodenolide$ inhibited ${\beta}-lactamase$ activity, 50, 100 and $200\;{\mu}M$ ${\beta}-liriodenolide$ did ${\beta}-lactamase$ activity as 0.7, 3.5 and 19.7%, respectively. The toxicity of ${\beta}-liriodenolide$ was not found with the method of photohemolysis.

• 한국자원식물학회지
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• 제24권1호
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• pp.23-29
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• 2011

본 연구는 새로운 기능성 소재를 탐색하기 위하여 튤립나무 가지의 메탄올 추출물을 조제하여 생리활성 물질 함량, DPPH, 환원능력, $Fe^{2+}$ chelating효과와 지질과산화 억제 활성 그리고 세포독성을 측정하였다. 총 페놀성 화합물과 플라보노이드 함량은 75.34 mg gallic acid/g과 20.15 mg quercetin/g이고; DPPH 라디칼 소거 활성에서 $EC_{50}$은 $289.68{\pm}2.04 \;{\mu}g$/mL; 환원력 측정에서 $100\;{\mu}g$/mL 농도에서 흡광도는 0.388이었으며, $Fe^{2+}$ chelating 효과에서는 $200\;{\mu}g$/mL 농도에서 36.33%로 나타내었고, 지질과산화 억제 효능에서는 $200\;{\mu}g$/mL의 농도에서 비교적 높은 38.56%의 억제율을 나타내었으며, 암세포 증식 억제 효과에서는 HT-29와 Hela cell line에서 튤립나무가지 메탄올 추출물이 $200\;{\mu}g$/mL의 농도에서 56.94%와 35.73%의 세포생장 억제율을 나타내었다. 따라서 본 연구 결과들을 종합해 볼 때 항산화능력은 총 페놀성분과 밀접한 관계가 있는 것으로 사료되며, 튤립나무 가지에 대한 기타 생리활성 연구가 더 필요할 것으로 사료된다.

• Journal of Plant Biotechnology
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• 제43권1호
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• pp.110-118
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• 2016

We compared germination efficiency for somatic embryos (SE) of Liriodendron tulipifera using semi-solid (SS), temporary immersion bioreactors (TIB), and continuous immersion bioreactors (CIB) to produce vigorous plants. The bioreactors were designed to be immersed in liquid media with plantlets with an adjustable immersion time. TIB and CIB improved germination rates up to 80.86% and 95.21%, respectively, however, CIB produced more hyperhydric plantlets than TIB. The height of plantlets in TIB was significantly higher than for those in CIB. Fresh weights of plantlets grown in CIB of were significantly lower than for those grown in TIB. The lowest chlorophyll concentration was found in in vitro plantlets from CIB. We examined abnormally developed leaves, stems, and apical zones of in vitro plantlets that were produced in CIB. Among the three types, SS showed the highest stomatal density and the shortest stomatal length in in vitro plantlets. After acclimatization, plants from CIB exhibited the lowest values in biomass, such as height, root collar diameter, leaf fresh weight, leaf length, leaf width, petiole length, petiole diameter, and leaf area. Photosynthesis and transpiration rates of ex vitro plants were not significantly different among the three culture types, but stomatal conductance was higher in TIB than in the SS and CIB. Therefore, the results suggest that TIB is the preferable bioreactor to improve in vitro plantlet regeneration of L. tulipifera. TIB-originated plants showed higher growth rate than SS and CIB after transferring to soil.

• 한국기후변화학회지
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• 제8권4호
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• pp.393-399
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• 2017

According to the United Nations Framework Convention on Climate Change (UNFCCC), all parties have to submit the national GHG inventory report. Estimating carbon stocks and changes in Land Use, Land-Use Changes and Forestry (LULUCF) needs an activity data and emission factors. So this study was conducted to develop carbon emission factor for Robinia pseudoacacia L., Betula platyphylla var. japonica, and Liriodendron tulipifera. As a result, the basic wood density ($g/cm_3$) was 0.64 for R. pseudoacacia, 0.55 for B. platyphylla, and 0.46 for L. tulipifera. Biomass expansion factor was 1.47 for R. pseudoacacia, 1.30 for B. platyphylla, and 1.24 for L. tulipifera. Root to shoot ratio was 0.48 for R. pseudoacacia, 0.29 for B. platyphylla, and 0.23 for L. tulipifera. Uncertainty of estimated emission factors on three species ranged from 3.39% to 27.43% within recommended value (30%) by IPCC. We calculated carbon stock and change using these emission factors. Three species stored carbon in forest and net $CO_2$ removal was $1,255,398\;t\;CO_2/yr$ during 5 years. So we concluded that our result could be used as emission factors for national GHG inventory report on forest sector.