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http://dx.doi.org/10.6116/kjh.2015.30.4.1.

Chemical composition, antioxidant potential and cyto-protecting activity of essential oil of Liriodendron tulipifera L. leaves  

Yadav, Anil Kumar (Department of Biotechnology, Daegu University)
Kim, Sang Ho (Department of Biology Education, Daegu University)
Kang, Sun Chul (Department of Biotechnology, Daegu University Department of Biotechnology, Daegu University Department of Biology Education, Daegu University Department of Biotechnology, Daegu University Department of Biology Education, Daegu University)
Publication Information
The Korea Journal of Herbology / v.30, no.4, 2015 , pp. 1-9 More about this Journal
Abstract
Objectives : The present study was under taken to characterize chemical composition, antioxidant and cyto-protecting capacity of essential oil obtained from leaves of Liriodendron tulipifera L. Methods : Essential oil from the leafof L. tulipifera L. (EOLL) was extracted by hydro-distillation process and further its chemical composition was evaluated by GC-MS analysis. The in vitro antioxidant potential of the EOLL was determined by DPPH ●, ABTS ●+, superoxide and nitric oxide free radical scavenging activity using different concentrations in the range of 50-800 μg/mL. In addition, cyto-protecting property of the EOLLwas determined by MTT assay on Raw 264.7 macrophage cells challenged with hydrogen peroxide (H 2 O 2 ). Results : The result of GC-MS analysis showed presence of 34 volatile compounds, principally germacrene D, spathulenol, and α -cadinol in EOLL. The in vitro antioxidant assays of EOLL at the highest used concentration of 800 μg/mL showed 81.62, 84.29, 83.59 and 58.59% inhibition of DPPH ●, ABTS ●+, superoxide, and nitric oxide radicals, respectively. It also showed ferric reducing ability with 1310.04 mM Fe (II)/g of essential oil. The EOLL at three different concentrations (200, 400 and 800 μg/mL) protected the cells from H 2 O 2 -induced cell damage through scavenging intracellular ROS. Conclusion : The findings from the study suggest that essential oil isolated from leaves of L tulipifera L. is a potent sources of natural antioxidants, which could be used to treat the diseases associated with oxidative stress condition.
Keywords
Liriodendron tulipifera L.; GC-MS; oxidative stress; MTT assay; antioxidant; cyto-protection;
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