• Molecular & Cellular Toxicology
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• v.1 no.2
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• pp.87-91
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• 2005

Although 2D-PAGE has been widely used as the primary method for protein separation, difficulties in displaying proteins with an extreme values of isoelectric paint (pI), molecular size and hydrophobicity limit the technique. In addition, time consuming steps involving protein transfer and extraction from the gel-pieces can result in sample loss. Here, we describe a novel protein separation technique with capillary size-exclusion chromatography (CSEC) for rapid protein identification from human plasma. The method includes protein fractionation along with molecular size followed by in-solution tryptic digestion and peptide analysis through reversed phase liquid chromatography (RPLC) coupled to nanoflow electrospray-tandem mass spectrometry (ESI-MS/MS). Tryptic peptides are applied an a $100\;{\mu}m\;i.d.{\times}10mm$ length pre-column and then separated on a $75\;{\mu}m{\times}200mm$ analytical column at -100 nL/min flaw rate. Proteins were identified over the wide ranges of pI (3.7-12.3) when this technique was applied to the analysis of $1-2\;{\mu}L$ of human plasma. This gel-free system provides fast fractionation and may be considered a complementary technique to SDS-PAGE in proteomics.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.1
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• pp.1-11
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• 2002

Flow of fluid has been studied in various fields of fluid engineering. To hydraulic engineers, the unsteady flow such as pulsation and liquid hammering in pipes has been considered as a serious trouble. So we are supposed to approach the formalized mathematical model by using more exact momentum equation for fluid transmission lines. Most of recent studies fur pipe line have been studied without considerations of variation of viscosity and temperature, which are the main factors of pressure loss causing the friction of fluid inside pipe line. Frequency response experiments are carried out with use of a rotary sinusoidal flow generator to investigate wave equation take into account viscosity and temperature. But we observed that measured value of gains are reduced as temperature increased. And it was respectively observed that the measured value of gains are reduced and line width of gain is broadened out, when temperature was high in the same condition. As we confessed, pressure loss and phase delay are closely related with the length, diameter and temperature of pipe line. In addition, they are the most important factors, when we decide the momentum energy of working fluid.

• Journal of Korea Foundry Society
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• v.30 no.6
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• pp.210-216
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• 2010

The aim of this study is to characterize a thixoextruded 7075 Al wrought alloy bar in terms of its isotropic behavior through the optical microscope, mechanical test and electron back scattered diffraction. It is also discussed of the extrudability improvement for 7075 Al wrought alloy by thixoextrusion, with emphasis on controlling thixoextrusion parameters. Hot extrusion shows that the maximum extrusion pressure depends on their characteristics in terms of flow stress and hot workability. In the contrary, thixoextrusion demonstrates that the maximum extrusion pressure is almost uniform regardless of the experimental parameters, such as initial ram speed, die bearing length and thixoextrusion temperature. The hot extruded microstructures become elongated to extrusion direction, while the thixoextruded microstructures are isotropic and homogeneously distributed due to the existence of liquid phase between solid grains during the process. The grain refinement due to dynamic recrystallization during thixoextrusion has been also occurred. Subsequent recrystallization would lead to the strengthening of mechanical properties, as observed in the study. The important point is that the values of tensile, yield strength and elongation of the thixoextruded bar without plastic deformation are similar to those of the hot extruded bar with severe plastic deformation.

• Proceedings of the SAREK Conference
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• 2005.11a
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• pp.317-322
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• 2005

This study is aimed to analyze the effects of heat pipe shape on the heat transfer in solar collector with a axial grooved heat pipe. In the design of a heat pipe. two of the most important criteria to be met are the operating temperature range and the maximum heat transport capacity, When the operating temperature range is known and the working fluid has been selected, the maximum heat transport capacity depends strongly on capillary pressure and liquid flow. The heat transport capacity of the heat pipe will depend on the geometry of the heat pipe, the wick structure. the vapor channel shape. groove number. cooling temperature. condenser length and pipe diameter. So various shapes are used for mathematical models of two-phase flow in grooved heat pipe. From the results. the adequate groove shape and scale are presented by considering the heat transport and capillary limitation.

• Macromolecular Research
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• v.15 no.2
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• pp.160-166
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• 2007

Novel multiblock copolymers, based on segmented sulfonated hydrophilic-hydrophobic blocks, were synthesized and investigated for their application as proton exchange membranes. A series of segmented sulfonated poly(arylene ether sulfone)-b-polyimide multiblock copolymers, with various block lengths, were synthesized via the coupling reaction between the terminal amine moieties on the hydrophilic blocks and naphthalene anhydride functionalized hydrophobic blocks. Successful imidization reactions required a mixed solvent system, comprised of NMP and m-cresol, in the presence of catalysts. Proton conductivity measurements revealed that the proton conductivity improved with increasing hydrophilic and hydrophobic block lengths. The morphological structure of the multiblock copolymers was investigated using tapping mode atomic force microscopy (TM-AFM). The AFM images of the copolymers demonstrated well-defined nanophase separated morphologies, with the changes in the block length having a pronounced effect on the phase separated morphologies of the system. The self diffusion coefficient of water, as measured by $^1H$ NMR, provided a better understanding of the transport process. Thus, the block copolymers showed higher values than Nafion, and comparable proton conductivities in liquid water, as well as under partially hydrated conditions at $80^{\circ}C$. The new materials are strong candidates for use in PEM systems.

• Journal of Powder Materials
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• v.23 no.6
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• pp.409-413
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• 2016

Electrical wire explosion in liquid media is a promising method for producing metallic nanopowders. It is possible to obtain high-purity metallic nanoparticles and uniform-sized nanopowder with excellent dispersion stability using this electrical wire explosion method. In this study, Ni-Fe alloy nanopowders with core-shell structures are fabricated via the electrical explosion of Ni-Fe alloy wires 0.1 mm in diameter and 20 mm in length in de-ionized water. The size and shape of the powders are investigated by field-emission scanning electron microscopy, transmission electron microscopy, and laser particle size analysis. Phase analysis and grain size determination are conducted by X-ray diffraction. The result indicate that a core-shell structured Ni-Fe nanopowder is synthesized with an average particle size of approximately 28 nm, and nanosized Ni core particles are encapsulated by an Fe nanolayer.

• Journal of the Korean Chemical Society
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• v.28 no.3
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• pp.194-202
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• 1984

The adsorption properties of organo sulfur compounds on cation exchanged natural zeolites from n-heptane were investigated. The equilibrium adsorbed amounts were dependent upon the exchanged cation and the nature of organo sulfur compounds such as length, volume, electronical structure. The increasing orders of equilibrium adsorbed amounts were thiophene derivatives, disulfide, sulfide mercaptane and thiophene, benzothiaphene, dibenzothiophene. And $Co^{+2}$-zeolite was the most prominent adsorbant. Rate determining step of the adsorption at initial stage was intraparticle diffusion into the transitional pores of zeolite. These adsorption rates were dependent upon the bulkiness of adsorbate. Finally, preadsorbed water didn't affect these adsorption until the cation exchanged natural zeolite contained 2.26${\times}10^{-3}$ mol/g of water. It indicated that water preferentially occupied the micro pores of the cation exchanged natural zeolites.

• Journal of the Korean Ceramic Society
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• v.51 no.2
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• pp.107-114
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• 2014

In the present work, we report a novel microstructure of scalenohedral calcite synthesized without any additives by a simple and ecofriendly carbonation process carried out in a liquid-gas system as well as the effects of experimental conditions on the crystal growth of the scalenohedral calcite phase. Various process parameters, pH, temperature, $Ca(OH)_2$ concentration, $CO_2$ flow rates, and the total volume concentration, were investigated to enhance the sensitivity of the process. The highest average length of the scalenohedral calcite was obtained at pH 6.0, temperature of $45^{\circ}C$, $Ca(OH)_2$ concentration of 0.2M, $CO_2$ flow rate of 80mL/min, and total volume of 1L. The synthesized calcite was characterized by XRD, SEM, and FTIR to identify the phases and surface morphology.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.19 no.2
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• pp.156-161
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• 2007

A set of experiments of counter-current flow limit (CCFL) was performed to improve the drawbacks of Wallis' correlation which neglected the effects of channel size, channel length, injection method and the boundary conditions at the inlet of liquid and gas phase. In this study, CCFL was observed by changing the shape of porous plate using air and water. The results show that as the size of porous increases, CCFL with a round shape of the porous plate start to disappear, In this study, the CCFL correlation was calculated and the corresponding CCFL map was developed based on the experimental results.

• Korean Journal of Materials Research
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• v.12 no.5
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• pp.398-406
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• 2002

VAR process is required to control its various operating parameters. Heat transfer simulation has been accomplished to understand development of solidification micro and macro-structures during VAR process in Ti alloys. Optimum VAR process parameters could be also estimated in this study. It was found that macro-structures were closely related to the shape and depth of liquid pool, and solidification parameters, such as temperature gradient, heat flux, solid fraction. The cooling rates were higher at bottom, top, and center part respectively. As cooling rates increased, the $\alpha$ phase decreased in length, width and fraction. In order to evaluate which parameter affects the result of heat transfer calculation most sensitively, the sensitivities of input parameters to the simulation result were examined. The pool depth and cooling rate showed more sensitive to the temperature of the molten metal, heat transfer coefficient, and liquidus respectively. Also, these thermal properties became more sensitive at higher temperatures.