• Title/Summary/Keyword: Liquid transfer

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Determination of Analytical Approach for Ambient PM2.5 Free Amino Acids using LC-MSMS (LC-MSMS를 이용한 대기 중 PM2.5 유리아미노산 분석 방법 연구)

  • Bae, Min-Suk;Park, Da-Jeong;Lee, Kwon-Ho;Cho, Seung-Sik;Lee, Kwang-Yul;Park, Kihong
    • Journal of Korean Society for Atmospheric Environment
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    • v.33 no.1
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    • pp.54-63
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    • 2017
  • Atmospheric nitrogen containing organic compounds(e.g. amino acids) has attracted considerable attention from the viewpoint of the oceanic biogeochemical cycle of nitrogen as well as the long range transfer. However, only a few measurements of organic nitrogen compounds have been conducted due to analytical difficulties. In this study, total of nine amino acids such as Glutamic acid, Histidine, Arginine, Tyrosine, Cystine, Valine, Methionine, Phenylalanine, Lysine have been analytically determined by Liquid Chromatography - Mass Spectrometry Mass Spectrometry (LC-MSMS). As results, Fragmentor Voltage (FV), Precursor Ion, Collision Energy, Product Ion related to individual amino acid compounds are shown. Based on the operational conditions, Lysine, Glutamine Acid, Tyrosine were analyzed during the China Oriented Smog Period. High concentrations of Lysine, Glutamine Acid, and Tyrosine are discussed with organic carbon (OC), elemental carbon (EC), and water soluble ions. The results can provide to understand the sources with aging process related to amino acids influenced by the long-range transport from the Yellow Sea area.

Study on the Systematic Technology of Promoting Purification for the Livestock Wastewater and Reuse

  • Okada, Yoshiichi;Shim, Jae-Do;Mitarai, Masahumi;Kojima, Takayuki;Gejima, Yoshinori
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1996.06c
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    • pp.692-700
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    • 1996
  • The objective of this study is to develop a systematic purification plant using the metabolism of aerobic microorganisms. This system is subsequently aerated and continuously removes suspended solids and settling sludges caused by aerating pressure at the bottom of a lower pipe (i.e., Continuous Removal of Suspended solids and Settling sludges, CRSS). The CRSS plants are brought out by introducing fine air bubbles into the liquid phase of a lower pipe in the bio-reactor. These plant uses aeration pipe, with multiple inlets to sweep the floor of bio-reactor tank, instead of the conventional scraper mechanisms. The principal advantage of this system is that it can continuously remove very small or light particles that settles completely within a short time. Once the particles have been floated to the surface, they can be moved into the pipe and collected in the settling tank by sequently aerated pressure. The experimental results shows that about 99.0% of the biochemical oxygen demand(BOD), 99.3% of the suspended solid(SS), 92.3% of the total nitrogen(T-N), 99.0% of the turbidity(TU), 100% of the total coliform(TC)and ammonia was respectively removed during aerobic digestion for 9 days. These result indicates that the CRS S plants are very effective for reduction and deodorization of swine wastewater contaminants, and the efflux from CRS S can either be discharged in the river or used as nutrient solution of formulation for plant growth factories. The developed CRSS plant proved to be flexible and it can simply be adapted to any type of biological waste treatment problem.roblem.

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A Study on Propulsion Performance of Underwater Ram-Jet with Optimized Nozzle Configuration (최적 노즐형상을 갖는 수중램제트의 추진성능에 관한 연구)

  • Kang, H.K.;Kim, Y.T.;Lee, Y.H.
    • Journal of the Society of Naval Architects of Korea
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    • v.34 no.4
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    • pp.42-52
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    • 1997
  • The basic principle of underwater ram-jet as a unique marine propulsion concept showing vary high cruise speed range(e. g. 80-100 knots) is the thrust production by the transfer of the potential energy of compressed gas to the operating liquid through kinetic mixing process. This paper is aimed to investigate the propulsive efficiency of the nozzle flow in underwater ram-jet at the speed of 80 knots for the buried type vessel. The basic assumption of the theoretical analysis is that mixture of water and air can be treated as incompressible gas. For an optimized nozzle configuration obtained from the performance analysis, preliminary data for performance evaluation are obtained and effects of nozzle inner wall friction, ambient temperature, ambient pressure, water density, gas velocity, bubble radius, flow velocity, diffuser area ratio, mass flow ratio and water velocity gradient are investigated.

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Embryotoxicity and Toxicokinetics of the Antimalarial Artesunate in Rats

  • Chung, Moon-Koo;Yu, Wook-Joon;Lee, Jin-Soo;Lee, Jong-Hwa
    • Toxicological Research
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    • v.29 no.1
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    • pp.27-34
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    • 2013
  • This study was conducted to investigate the potential embryo-fetal toxicity and toxicokinetics of the antimalarial agent artesunate (ARTS) in Sprague-Dawley rats. Pregnant rats were administered ARTS daily from gestational day 6~15 via oral gavage, at test doses of 0, 2, 4, or 8 mg/kg (22 females per group). The fetuses were examined for external, visceral, and skeletal abnormalities on gestational day 20. With regard to the dams, there were no deaths, treatment-related clinical signs, changes in body weight, or food intake in any of the treatment groups. There were no treatment-related gross findings at necropsy in any treatment group. In the 8 mg/kg group, there was a decrease in gravid uterine weight and in the weight of female fetuses. There was also an increase in fetal deaths (primarily late resorptions) and an increase in post-implantation losses (37%) at 8 mg/kg. An increase in the incidence of visceral and skeletal variations at 4 and 8 mg/kg was observed. These defects included minor changes in the appearance of the kidney and thymus, as well as absent ribs or thoracic vertebrae. Toxicokinetics were assessed in a parallel study, using 4 mated females per group. Using liquid chromatography-mass spectrometry (LC-MS) analysis, the concentration of ARTS and its metabolite dihydroartemisinin (DHA) were quantified in plasma from rats on gestational days 5, 6, 10, and 15. Amniotic fluid was assayed for ARTS and DHA on gestational day 15. There was evidence of rapid conversion of ARTS to the metabolite DHA in maternal plasma, since ARTS could not be consistently detected in plasma at the three doses tested. ARTS and DHA were not detected in amniotic fluid at gestational day 15, indicating limited placental transfer of the two agents. The embryo-fetal no-observable-adverse-effect level (NOAEL) of the test item was considered to be 8 mg/kg/day for dams, and 2 mg/kg/day for embryo-fetal development.

Somatic Embryogenesis and Plant Regeneration in Immature Flower Bud Cultures of Carnation (카네이션의 미숙화뢰 배양을 통한 체세포배 발생 및 식물체 재분화)

  • 안병준
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.6
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    • pp.369-374
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    • 1997
  • Immature flower buds of 'Desio' carnation were cultured on MS agar medium supplemented with 1 ㎎/L 2,L-D. Embryogenic calli were formed from 5-10% of the buds less than 20 ㎜ in length, but only non-embryogenic calli were produced from explants of shoot apex leaf, internode, and flowere buds larger than 20 ㎜. The same method was applied to 16 cultivars of cut Sower carnation and embryogenic calli were obtained in 7 cultivars. Several embryogenic callus lines were selected and maintained through subcultures over 120 weeks without loss of embryogenic competence. The embryogenic cultures were also proliferated rapidly in liquid agitation cultures using MS medium supplemented with 1mg/L 2,4-D. Numerous embryos were formed on the periphery of the cell aggregates upon transfer to auxin-free MS agar medium. Plantlets were transplanted in potting soil and grown to bloom in six months.

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Effects of Electromagnetic Heating on Quick Freezing

  • Kim, Jinse;Park, Jong Woo;Park, Seokho;Choi, Dong Soo;Choi, Seung Ryul;Kim, Yong Hoon;Lee, Soo Jang;Yoo, Seon Mi;Han, Gui Jeung
    • Journal of Biosystems Engineering
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    • v.40 no.3
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    • pp.271-276
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    • 2015
  • Purpose: Quick freezing is widely used in commercial food storage. Well-known freezing techniques such as individual quick freezing require a low-temperature coolant and small cuts for the heat-transfer efficiency. However, the freezing method for bulk food resembles techniques used in the 1970s. In this study, electromagnetic (EM) heating was applied to improve the quick freezing of bulk food. Methods: During freezing, the surface of food can be rapidly cooled by an outside coolant, but the inner parts of the food cool slowly owing to the latent heat from the phase change. EM waves can directly heat the inner parts of food to prevent it from freezing until the outer parts finish their phase change and are cooled rapidly. The center temperature of garlic cloves was probed with optical thermo sensors while liquid nitrogen (LN) was sprayed. Results: When EM heating was applied, the center cooling time of the garlic cloves from freezing until $-10^{\circ}C$ was 48 s, which was approximately half the value of 85 s obtained without EM heating. For the white radish cubes, the center cooling time was also improved, from 288 to 132 s. The samples frozen by LN spray with EM heating had a closer hardness to the unfrozen samples than the samples frozen by LN only. Conclusions: The EM heating during quick freezing functions to maintain the hardness of fresh food by reducing the freezing time from 0 to $-10^{\circ}C$.

Prediction of Glaze Ice Accretion on 2D Airfoil (2차원 에어포일의 유리얼음 형상 예측 코드 개발)

  • Son, Chan-Kyu;Oh, Se-Jong;Yee, Kwan-Jung
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.38 no.8
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    • pp.747-757
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    • 2010
  • The ice accreted on the airfoil is one of the critical drivers that causes the degradation of aerodynamic performance as well as aircraft accidents. Hence, an efficient numerical code to predict the accreted ice shape is crucial for the successful design of de-icing and anti-icing devices. To this end, a numerical code has been developed for the prediction of glaze ice accretion shape on 2D airfoil. Constant Source-Doublet method is used for the purpose of computational efficiency and heat transfer in the icing process is accounted for by Messinger model. The computational results are thoroughly compared against available experiments and other computation codes such as LEWICE and TRAJICE. The direction and thickness of ice horn are shown to yield similar results compared to the experiments and other codes. In addition, the effects of various parameters - temperature, free-stream velocity, liquid water contents, and droplet diameter - on the ice shape are systematically analyzed through parametric studies.

A Study on Absorber in Absorption Heat Pump with Methanol-Glycerine System as a Working Fluid (메탄올-글리세린계를 작동유체로 하는 흡수열펌프에서 흡수기 연구)

  • Min, Byong-Hun
    • Applied Chemistry for Engineering
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    • v.17 no.1
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    • pp.111-117
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    • 2006
  • The improvement of energy conservation mandates decrease consumption of fossil fuels and minimize negative impacts on the environment, which originates from large cooling and heating demand. The absorption heat pump technology has a large potential for energy saving in this respect. Adsorption heat pump is a means to upgrade waste heat without addition of extra thermal energy. The increase of absorbed amount is of great importance for absorption heat pump cycle. In this study, in order to improve the performance of absorber, the absorbers of two different types have been investigated using methanol-glycerine as a working fluid. The former was tangential feed of liquid phase without spiral tube in the absorber and the latter was with spiral tube in the absorber. The latter was found to be more effective in enhancing the mass and heat transfer to increase the absorption performance.

Preparation and Luminescent Properties of LaPO4:Re (Re=Er, Yb) Nanoparticles (희토류 이온(Er/Yb)이 도핑된 LaPO4 나노입자의 합성과 발광특성)

  • Oh Jae-Suk;Lee Tack-Hyuck;Seok Sang-Il;Jung Ha-Kyun
    • Korean Journal of Materials Research
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    • v.14 no.4
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    • pp.270-275
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    • 2004
  • Due to the luminescence by$ Er ^{ 3+}$ activator, Er-doped $LaPO_4$ powders can be applied for optical amplification materials. In this study, $LaPO_4$:Er nanoparticles were synthesized in solution system using a high-boiling coordinating solvent and their properties were investigated through various spectroscopic techniques. The nanoparticles were to take a single phase of monazite structure by a X-ray diffraction analysis and to have the 5-6 nm of particles size with narrow size distribution by a TEM. And it was confirmed by the EA and FT-IR analyses that the surfaces of nanoparticles are coordinated with the solvent molecules, which will possibly keep from agglomerating between LaPO$_4$:Er nanoparticles. In the emission spectrum of $LaPO_4$:Er nanoparticle at NIR region, on the other hand, it was measured that the emission intensity is very weak, which is due to the transition from $^4$$I_{(13/2)}$ to $^4$$I_{(15/2)}$ of $Er^{3+ }$ion. It was interpreted that the weak luminescence of $LaPO_4$:Er is originated from the hydroxyl groups adsorbed on the surfaces of the nanoparticles, because OH group acts as an efficient quencher for the $^4$$I_{(13/2)}$ \longrightarrow $^4$$I_{(15/2)}$ emission of $Er^{3+}$ activator. But the co-doping of Yb$^{3+}$ as a sensitizer in this nanoparticle results in the increase of the emission intensity at 1539 nm due to the effective energy transfer from $Yb^{3+}$ to $Er^{3+}$ . In addition, the synthesized nanoparticles exhibited good dispersibility with some polymers and effective luminescence at NIR region.n.

Establishment and Characterization of Clonal Cell Lines from Zebrafish, Danio rerio (제브라피쉬(Danio rerio) 배아로부터 동형세포주 확립)

  • Lee, Ki-Young
    • Korean Journal of Ichthyology
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    • v.20 no.1
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    • pp.1-6
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    • 2008
  • Three types of clonal cell lines were isolated according to their size and phenotype from the adherent cell populations in long-term liquid cultures from the embryonic fibroblast cells of Zebrafish, Danio rerio. All kind of cell lines were well proliferated. The size and number of clonal cell lines derived colonies from stable embryonic cells were significantly increased in the presence of NAC and A2P conditioned medium from the cell lines. The stable cell lines and clonal cell lines were cap-able of well proliferation in vitro. These cell lines have been maintained in continuous culture without change in characteristics. A majority of the clonal cells (80%) was shown a normal chromosomal complement (50 chromosomes, 2N) in according with FACs analysis. Majority of cells were positive to vimentin staining and none of them were positive for nestin and Oct -4 by immunocytochemistry. These results indicate that the clonal cell lines obtained from cultured cells are fibroblasts and may be extremely useful in genetic manipulation for further nuclear transfer and fish cloning.