• Title/Summary/Keyword: Liquid transfer

검색결과 1,332건 처리시간 0.033초

Characterization of the Biogenic Manganese Oxides Produced by Pseudomonas putida strain MnB1

  • Jiang, Shaofeng;Kim, Do-Gun;Kim, Jeong-Hyun;Ko, Seok-Oh
    • Environmental Engineering Research
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    • 제15권4호
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    • pp.183-190
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    • 2010
  • Biogenic Mn oxides are expected to have great potential in the control of water pollution due to their high catalytic activity, although information on biological Mn oxidation is not currently sufficient. In this study, the growth of a Mn oxidizing microorganism, Pseudomonas putida MnB1, was examined, with the Mn oxides formed by this strain characterized. The growth of P. putida MnB1 was not significantly influenced by Mn(II), but showed a slightly decreased growth rate in the presence of Pb(II) and EE2, indicating their insignificant adsorption onto the cell surface. Mn oxides were formed by P. putida MnB1, but the liquid growth medium and resulting biogenic solids were poorly crystalline, nano-sized particles. Biogenic Mn oxidation by P. putida MnB1 followed Michaelis-Menten kinetics, with stoichiometric amounts of Mn oxides formed, which corresponded with the initial Mn(II) concentration. However, the formation of Mn oxides was inhibited at high initial Mn(II) concentration, suggesting mass transfer obstruction of Mn(II) due to the accumulation of Mn oxides on the extracellular layer. Mn oxidation by P. putida MnB1 was very sensitive to pH and temperature, showing sharp decreases in the Mn oxidation rates outside of the optimum ranges, i.e. pH 7.43-8.22 and around 20-$26^{\circ}C$.

LC-MSMS를 이용한 대기 중 PM2.5 유리아미노산 분석 방법 연구 (Determination of Analytical Approach for Ambient PM2.5 Free Amino Acids using LC-MSMS)

  • 배민석;박다정;이권호;조승식;이광열;박기홍
    • 한국대기환경학회지
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    • 제33권1호
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    • pp.54-63
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    • 2017
  • Atmospheric nitrogen containing organic compounds(e.g. amino acids) has attracted considerable attention from the viewpoint of the oceanic biogeochemical cycle of nitrogen as well as the long range transfer. However, only a few measurements of organic nitrogen compounds have been conducted due to analytical difficulties. In this study, total of nine amino acids such as Glutamic acid, Histidine, Arginine, Tyrosine, Cystine, Valine, Methionine, Phenylalanine, Lysine have been analytically determined by Liquid Chromatography - Mass Spectrometry Mass Spectrometry (LC-MSMS). As results, Fragmentor Voltage (FV), Precursor Ion, Collision Energy, Product Ion related to individual amino acid compounds are shown. Based on the operational conditions, Lysine, Glutamine Acid, Tyrosine were analyzed during the China Oriented Smog Period. High concentrations of Lysine, Glutamine Acid, and Tyrosine are discussed with organic carbon (OC), elemental carbon (EC), and water soluble ions. The results can provide to understand the sources with aging process related to amino acids influenced by the long-range transport from the Yellow Sea area.

Study on the Systematic Technology of Promoting Purification for the Livestock Wastewater and Reuse

  • Okada, Yoshiichi;Shim, Jae-Do;Mitarai, Masahumi;Kojima, Takayuki;Gejima, Yoshinori
    • 한국농업기계학회:학술대회논문집
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    • 한국농업기계학회 1996년도 International Conference on Agricultural Machinery Engineering Proceedings
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    • pp.692-700
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    • 1996
  • The objective of this study is to develop a systematic purification plant using the metabolism of aerobic microorganisms. This system is subsequently aerated and continuously removes suspended solids and settling sludges caused by aerating pressure at the bottom of a lower pipe (i.e., Continuous Removal of Suspended solids and Settling sludges, CRSS). The CRSS plants are brought out by introducing fine air bubbles into the liquid phase of a lower pipe in the bio-reactor. These plant uses aeration pipe, with multiple inlets to sweep the floor of bio-reactor tank, instead of the conventional scraper mechanisms. The principal advantage of this system is that it can continuously remove very small or light particles that settles completely within a short time. Once the particles have been floated to the surface, they can be moved into the pipe and collected in the settling tank by sequently aerated pressure. The experimental results shows that about 99.0% of the biochemical oxygen demand(BOD), 99.3% of the suspended solid(SS), 92.3% of the total nitrogen(T-N), 99.0% of the turbidity(TU), 100% of the total coliform(TC)and ammonia was respectively removed during aerobic digestion for 9 days. These result indicates that the CRS S plants are very effective for reduction and deodorization of swine wastewater contaminants, and the efflux from CRS S can either be discharged in the river or used as nutrient solution of formulation for plant growth factories. The developed CRSS plant proved to be flexible and it can simply be adapted to any type of biological waste treatment problem.roblem.

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최적 노즐형상을 갖는 수중램제트의 추진성능에 관한 연구 (A Study on Propulsion Performance of Underwater Ram-Jet with Optimized Nozzle Configuration)

  • 강호근;김유택;이영호
    • 대한조선학회논문집
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    • 제34권4호
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    • pp.42-52
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    • 1997
  • 수중램제트(underwater ram-jet)는 램흡입부(ram intake), 혼합실(mixing chamber) 및 노즐(nozzle)로 구성되어 있으며, 램흡입부로 유입된 작동유체는 압력이 증가되며 이 증압된 작동유체에 혼합실로부터 고압공기를 분사하여 기 액이상류를 형성하여 노즐을 통과하면서 대기압까지 팽창을 하여 작동유체를 고속으로 가속시켜 노즐출구로부터 추력을 얻는 방식으로 차세대 초고속 선박추진장치이다. 본 연구에서는 80노트를 낼 수 있는 선내관통형(buried type vessel) 램제트의 최적 노즐형상데이터를 이용하여 제반변수(벽마찰계수, 가스속도, 기포반경, 대기온도, 질량유량비, 디퓨저면적비, 작동유체의 속도구배)의 변화가 추진특성에 미치는 영향을 파악하였다.

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Embryotoxicity and Toxicokinetics of the Antimalarial Artesunate in Rats

  • Chung, Moon-Koo;Yu, Wook-Joon;Lee, Jin-Soo;Lee, Jong-Hwa
    • Toxicological Research
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    • 제29권1호
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    • pp.27-34
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    • 2013
  • This study was conducted to investigate the potential embryo-fetal toxicity and toxicokinetics of the antimalarial agent artesunate (ARTS) in Sprague-Dawley rats. Pregnant rats were administered ARTS daily from gestational day 6~15 via oral gavage, at test doses of 0, 2, 4, or 8 mg/kg (22 females per group). The fetuses were examined for external, visceral, and skeletal abnormalities on gestational day 20. With regard to the dams, there were no deaths, treatment-related clinical signs, changes in body weight, or food intake in any of the treatment groups. There were no treatment-related gross findings at necropsy in any treatment group. In the 8 mg/kg group, there was a decrease in gravid uterine weight and in the weight of female fetuses. There was also an increase in fetal deaths (primarily late resorptions) and an increase in post-implantation losses (37%) at 8 mg/kg. An increase in the incidence of visceral and skeletal variations at 4 and 8 mg/kg was observed. These defects included minor changes in the appearance of the kidney and thymus, as well as absent ribs or thoracic vertebrae. Toxicokinetics were assessed in a parallel study, using 4 mated females per group. Using liquid chromatography-mass spectrometry (LC-MS) analysis, the concentration of ARTS and its metabolite dihydroartemisinin (DHA) were quantified in plasma from rats on gestational days 5, 6, 10, and 15. Amniotic fluid was assayed for ARTS and DHA on gestational day 15. There was evidence of rapid conversion of ARTS to the metabolite DHA in maternal plasma, since ARTS could not be consistently detected in plasma at the three doses tested. ARTS and DHA were not detected in amniotic fluid at gestational day 15, indicating limited placental transfer of the two agents. The embryo-fetal no-observable-adverse-effect level (NOAEL) of the test item was considered to be 8 mg/kg/day for dams, and 2 mg/kg/day for embryo-fetal development.

카네이션의 미숙화뢰 배양을 통한 체세포배 발생 및 식물체 재분화 (Somatic Embryogenesis and Plant Regeneration in Immature Flower Bud Cultures of Carnation)

  • 안병준
    • 식물조직배양학회지
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    • 제24권6호
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    • pp.369-374
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    • 1997
  • 카네이션의 종묘 급속증식 기술 및 형질전환 연구의 기초 연구로서 체세포배발생 조직배양 방법을 개발하고자 수행되었다. 'Desio' 카네이션의 20 mm 이하의 미숙화뢰를 2,4-D가 1 ㎎/L 함유된 MS 기본배지에서 배양하면 배발생 캘러스가 5-l0%의 효율로 유도되었으나, 줄기 정단분열조직, 잎, 절간조직과 20 ㎜ 이상의 화뢰에서는 비배발생 캘러스만이 형성되었다. 동일한 배양방법을 16품종의 절화용 카네이션에 적용한 결과, 7품종에서 배발생 캘러스를 유도할 수 있었다. 일부 배발생 캘러스 세포주는 120주 이상 배발생능 상실 없이 계대배양을 통해 증식할 수 있었으며, 액체 진탕 배양을 통해 배발생 세포괴 상태로 급속 증식하는 배양체가 획득되었다. 배발생 캘러스 또는 액체배양 배발생 세포괴를 호르몬이 포함되지 않은 MS 한천배지에 이식하며 2-3주내에 체세포배 발생을 통하여 식물체가 재분화되었다. 토양이식된 식물체는 정상적으로 생장하여 20주 후 개화하였다.

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Effects of Electromagnetic Heating on Quick Freezing

  • Kim, Jinse;Park, Jong Woo;Park, Seokho;Choi, Dong Soo;Choi, Seung Ryul;Kim, Yong Hoon;Lee, Soo Jang;Yoo, Seon Mi;Han, Gui Jeung
    • Journal of Biosystems Engineering
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    • 제40권3호
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    • pp.271-276
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    • 2015
  • Purpose: Quick freezing is widely used in commercial food storage. Well-known freezing techniques such as individual quick freezing require a low-temperature coolant and small cuts for the heat-transfer efficiency. However, the freezing method for bulk food resembles techniques used in the 1970s. In this study, electromagnetic (EM) heating was applied to improve the quick freezing of bulk food. Methods: During freezing, the surface of food can be rapidly cooled by an outside coolant, but the inner parts of the food cool slowly owing to the latent heat from the phase change. EM waves can directly heat the inner parts of food to prevent it from freezing until the outer parts finish their phase change and are cooled rapidly. The center temperature of garlic cloves was probed with optical thermo sensors while liquid nitrogen (LN) was sprayed. Results: When EM heating was applied, the center cooling time of the garlic cloves from freezing until $-10^{\circ}C$ was 48 s, which was approximately half the value of 85 s obtained without EM heating. For the white radish cubes, the center cooling time was also improved, from 288 to 132 s. The samples frozen by LN spray with EM heating had a closer hardness to the unfrozen samples than the samples frozen by LN only. Conclusions: The EM heating during quick freezing functions to maintain the hardness of fresh food by reducing the freezing time from 0 to $-10^{\circ}C$.

2차원 에어포일의 유리얼음 형상 예측 코드 개발 (Prediction of Glaze Ice Accretion on 2D Airfoil)

  • 손찬규;오세종;이관중
    • 한국항공우주학회지
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    • 제38권8호
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    • pp.747-757
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    • 2010
  • 날개위에 쌓인 얼음은 날개의 공기역학적 성능을 저하시키고 항공기 사고를 야기하는 주요한 원인이 된다. 결빙된 날개의 형상 예측 연구는 얼음으로 변형된 날개의 공기역학적 성능 파악과 제빙 방빙 장치 설계에 선행 되어야 한다. 이를 위해 본 연구에서는 2차원 익형에 발생하는 유리얼음 형상을 예측할 수 있는 코드를 개발하였다. 계산의 효율성을 위해 익형 주위의 유동장 계산은 정상 용출 및 중첩 패널기법을 사용하였고 날개표면과 날개에 유입된 물의 열전달은 Messinger 모델을 통해 고려하였다. 본 코드의 해석 결과는 실험 및 LEWICE 등 기존 해석 프로그램과 상호 검증되었다. 결빙형상의 주요한 요소인 얼음 뿔의 진행 방향 및 얼음 두께는 실험 및 타 프로그램과 유사한 결과를 나타내었다. 또한 대기온도, 수증기 함량, 입자 반지름, 자유류 등이 착빙 형상에 미치는 영향을 체계적으로 분석하였다.

메탄올-글리세린계를 작동유체로 하는 흡수열펌프에서 흡수기 연구 (A Study on Absorber in Absorption Heat Pump with Methanol-Glycerine System as a Working Fluid)

  • 민병훈
    • 공업화학
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    • 제17권1호
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    • pp.111-117
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    • 2006
  • 냉 난방 수요에서 일어나는 환경오염의 최소화와 화석연료 소비를 감소시키기 위해서 에너지보존을 개선시키는 것은 필수적이다. 이러한 점에서 흡수식 열펌프기술은 에너지 절약을 위해서 많은 가능성을 가지고 있다. 흡수식 열펌프는 에너지를 주입하지 않고 폐열의 이용을 높일 수 있는 방법이다. 흡수식 열펌프는 흡수기에서 흡수된 양의 증가가 매우 중요하기 때문에 흡수기 성능이 매우 중요하다. 본 연구에서는 흡수기의 성능을 개선시키기 위해서 메탄올과 글리세린을 작동유체로 하는 두 종류의 흡수기에 관한 연구를 수행하였다. 전자는 흡수기 내에 액상을 접선방향으로 공급하는 것이고 후자는 흡수기 내벽에 나선형 관을 설치하여 액상을 접선방향으로 공급하는 것이다. 실험 결과 후자가 흡수기에서 발생하는 열 및 물질전달이 향상되어 흡수성능이 증가되었음을 알 수 있었다.

희토류 이온(Er/Yb)이 도핑된 LaPO4 나노입자의 합성과 발광특성 (Preparation and Luminescent Properties of LaPO4:Re (Re=Er, Yb) Nanoparticles)

  • 오재석;이택혁;석상일;정하균
    • 한국재료학회지
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    • 제14권4호
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    • pp.270-275
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    • 2004
  • Due to the luminescence by$ Er ^{ 3+}$ activator, Er-doped $LaPO_4$ powders can be applied for optical amplification materials. In this study, $LaPO_4$:Er nanoparticles were synthesized in solution system using a high-boiling coordinating solvent and their properties were investigated through various spectroscopic techniques. The nanoparticles were to take a single phase of monazite structure by a X-ray diffraction analysis and to have the 5-6 nm of particles size with narrow size distribution by a TEM. And it was confirmed by the EA and FT-IR analyses that the surfaces of nanoparticles are coordinated with the solvent molecules, which will possibly keep from agglomerating between LaPO$_4$:Er nanoparticles. In the emission spectrum of $LaPO_4$:Er nanoparticle at NIR region, on the other hand, it was measured that the emission intensity is very weak, which is due to the transition from $^4$$I_{(13/2)}$ to $^4$$I_{(15/2)}$ of $Er^{3+ }$ion. It was interpreted that the weak luminescence of $LaPO_4$:Er is originated from the hydroxyl groups adsorbed on the surfaces of the nanoparticles, because OH group acts as an efficient quencher for the $^4$$I_{(13/2)}$ \longrightarrow $^4$$I_{(15/2)}$ emission of $Er^{3+}$ activator. But the co-doping of Yb$^{3+}$ as a sensitizer in this nanoparticle results in the increase of the emission intensity at 1539 nm due to the effective energy transfer from $Yb^{3+}$ to $Er^{3+}$ . In addition, the synthesized nanoparticles exhibited good dispersibility with some polymers and effective luminescence at NIR region.n.