• Journal of Nutrition and Health
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• v.27 no.5
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• pp.442-450
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• 1994

The purpose of this study was to determine the effects of 2-AAF injection time on hepatic lipid peroxide metabolism and cytochrome P450 content in Sprague-Dawley rats fed diets containing high amounts of vegetable oils or animal fats(15%, w/w). Fifty mg of 2-AAF/kg of body weight/day was injected in PEG 300 intraperitonially for 3 consecutive days after 4 or 8 weeks to rats fed corn oil(CO) or lard(LA) diet. The contents of lipid peroxide and cytochrome P450, and the activities of superoxide dismutase(SOD), glutathione peroxidase(GSH-peroxidase) and glutathione S-transferase(GSH-S-transferase) were determined in hepatic microsomal or cytosolic fraction. Microsomal thiobarbituric acid reactive substances(TBARS) and cytochrome P450 contents increased in Co group injected 2-AAF after 4weeks. Cytosolic SOD activity increased in CO group injected 2-AAF after 4 weeks and in LA group injected 2-AAF after 4 or 8 weeks. Cytosolic GSH-S-transferase activity increased in LA group compared to CO group without 2-AAF injection. GSH-S-transferase activity increased in CO group injected 2-AAF after 4 or 8 weeks and in LA group injected 2-AAF after 4 weeks. Therefore, it may be suggested that 2-AAF injection increase the contents of lipid peroxide or cytochrome P450, and detoxifying enzyme activities in rats fed CO diet for short period and in rats fed LA diet for longer period.

• Environmental Analysis Health and Toxicology
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• v.11 no.3_4
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• pp.69-73
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• 1996

Brazilin was tested for its ability to inhibit alloxan induced lipidperoxidation. Lipid peroxide contents in liver, kidney and serum were measured by the TBA method. ICR mice receiving alloxan at a dose of 43mg/kg via the tail vein after a 24 hrs starvation showed significantly increased lipid peroxide contents as compared to untreated control. Lipid peroxide contents in liver, kidney and serum of alloxan-induced diabetic mice were dosedependently decreased by the treatment of brazilin at a dose of 10mg/kg, 50mg/kg, 100 mg/kg for 5 days.

• Journal of Haehwa Medicine
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• v.10 no.1
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• pp.483-487
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• 2001

Morus alba extract(MAE) was tested for its ability to inhibit alloxan induced lipidperoxidation. Lipid peroxide contents in serum, liver, kidney and heart were measured by the TBA method. ICR mice receiving alloxan at a dose of 6mg/kg intraperitoneally after a 24hrs starvation showed significantly increased lipid peroxide contents as compared to untreated control. Lipid peroxide contents in serum, liver, kidney of alloxan-diabetic mice were decreased by the treatment of MAE at the dose of 50mg/kg, 100mg/kg for 7 days.

• The Journal of Internal Korean Medicine
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• v.22 no.1
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• pp.53-61
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• 2001

Objectives : The following are the results of the experimental studies of Samjasan (SJS) extract on the nitric oxide synthase (NOS) activity and the level of lipid peroxide in the penises of rats. Methods : Cnidii Fructus, Cuscutae Semen, Schizandrae Fructus constitute SJS. 150 g of crushed crude drug was extracted with methyl alcohol, under reflux, for 24 hours, three times; the total extractive was evaporated under reduced pressure to give 28.6 g. Results : In vitro, the SJS extract didn't effect the activity of NOS. However, the SJS extract decreased the activities, the ratio of type conversion of xanthine oxidase, the levels of lipid peroxide, In vitro, after administration of the SJS extract to rats, the activities and ratio of type conversion of xanthine oxidase decreased, but the activity of NOS and the content of nitrite increased. Also, the levels of the superoxide anion radical and lipid peroxide decreased in the penises of rats. But, after administration of the SJS extract to rats, the levels of glutathione did not increase. The effects of the SJS extract did better as the dosage and the length of treatment increased. Conclusions : These results suggest that the SJS extract decreases the activities of free radical generating enzymes which form lipid peroxide and increases the NOS activity in the penises of rats. Therefore, the SJS extract is capable of improving of sexual ability in rats.

• The Korea Journal of Herbology
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• v.20 no.2
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• pp.127-136
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• 2005

Objectives : The present study attempted to reveal the effects of the combination of Fel Ursi and Bezoar on the improvement of the function of the liver, through examining the effects of Fel Ursi, Bezoar, and Woong-Whang Tang composed of Fel Ursi and Bezoar. Method : Acute hepatitis was induced by galactosamine to rats, and then Fel Ursi, Bezoar Bovis, and Woong-Whang Tang were introjected to measure the influence of serums such as AST, ALT, ALP, ${\gamma}GT$, and lipid peroxide of liver tissues which are considered as the index of the function of the liver. Results : 1. Fel Ursi decreased the lipid peroxide of ALT, ${\gamma}FT$, and liver tissue and that of mitochondria in liver tissues of the rats with galactosamine-induced acute hepatitis, but it did not have any significant effect on AST and ALP. 2. Bezoar Bovis decreased the lipid peroxide of AST, ALT, ALP, ${\gamma}GT$, and the liver tissues among serum of the rats suffering from acute hapatitis induced by galactosamine, but it did not have any significant effect on that of mitochondria of the liver tissues. 3. Woong-Whang Tang had a significant effects on the lipid peroxide of AST, ALT, ALP, ${\gamma}GT$, and the liver tissues, and on the decrease of lipid peroxide of mitochondria, among serum of the rats suffering from acute hapatitis induced by galactosamine. Conclusion : Fel Ursi and Bezoar Bovis were judged to be effective on the acute hepatitis of the liver by galactosamine. In particular, Woong-Whang Tang which was composed of the combination of Fel Ursi and Bezoar Bovis was more efficient in the improvement of the function of the liver and the amount of lipid peroxide than the respective use of Fel Ursi or Bezoar Bovis.

• Journal of Nutrition and Health
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• v.25 no.2
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• pp.116-122
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• 1992

This study determined hepatic microsomal lipid peroxide values glucose 6-phosphatase NA-DPH-cytochrome P450 reductase and cytosolic glutathione S-transferase activites to examine the effects of methyl group deficiency on hepatic lipid peroxidation in rats treated with diethylni-trosamine(DEN) and 2-acetylamionfluorene(AAF) Weanling sprague Dawley male rats were fed the diet with methyl group supplemented or deficient. Two weeks after feeding rate were injected with a single of 200mg/kg body weight DEN intraperitoneally and after four weeks 0.02% AAF containing diets were fed for two weeks. Animals were sacrificed at 6th week. Microsomal lipid peroxide values were tended to increase in methyl group deficiency(MD). Especially in case of carcinogen tratments lipid peroxide values were increased significantly in MD. Microsomal glucose 6-phophatase activities were decreased by MD and carcinogens and in MD with carcinogen group (MD+C) the enzyme activites were the lowest Glucose 6-phosphatase activities were negatively correlated with lipid peroxidation. Microsomal NADPH-cytochrome P450 reductase activities were the highest in MD+C and correlated positively with lipid peroxidation. Cytosolic glutathione S-transferase activities were the highest in MD+C Methyl group deficiency induces lipid peroxidation especially in case of being exposed to carcinogens. Therefore the results suggest that lipid peroxidation may be one of the meachanisms of carcinogensis by methyl group deficiency.

• Journal of Nutrition and Health
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• v.30 no.9
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• pp.1109-1115
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• 1997

The present study was aimed to investigated whether there are age-and sex-related differences in serum levels of lipid peroxides, retinol , and $\alpha$-tocopherol in Korean adults. The subjects were 441 persons , including 268 men and 173 women. Those of each sex were divided into four age group : 20-29, 30-39, 40-49 and 50-65 years, and their lifestyles and serum levels were compared . Men smoked and drank less as age increased , but 99.9% of women of did not smoke and only 11% drank more than once a month. Lipid peroxides of males increased gradually with age, while those of females showed greater levels in the 50-65 years group thon younger groups. Lipid peroxides, retinol and $\alpha$-tocopherol concentrations, which were adjusted for age, were significantly higher in males than in females. Lipid peroxide levels adjusted for total lipid were positively correlated with age in males than in females. Serum levels of $\alpha$-tocopherol adjusted for total lipid were positively correlated with age both in males and in females , while retinol was neither. The results indicate that serum levels of lipid peroxides, retinol , and $\alpha$-tocopherol are affected by age and that the response could be different between males and females.

• Korean Journal of Oriental Medicine
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• v.2 no.1
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• pp.192-204
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• 1996

Cervus elaphus for herb-acupuncture solution (CEHAS) was tested for the effects of free radical generating enzyme and lipid peroxidation in rat's kidney. In vitro, levels of lipid peroxide in tissues of kidney were proportionally decreased to concentration of extracts prepared from CEHAS. They were much more decreased, when lipid peroxidation was induced with ferrous iron (FeII). Also, enzyme activities of xinthine oxidase were decreased. The ratio of type conversion of xanthine oxidase was lowered, two. But, it was not seen changes on enzyme activities of aldehyde oxidase. These results suggest that CEHAS decrease the activities of free radical generating enzymes such as xanthine oxidase which form lipid peroxide.

• The Journal of Internal Korean Medicine
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• v.22 no.1
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• pp.63-71
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• 2001

Objectives : Cordyceps Sinensis (CS) was tested for the effects of antioxidant enzymes and lipid peroxidation in the liver. Methods : We measured the changes in body weight, enzyme activity, lipid peroxide and the death rate in the hydrocortisone acetate-treated rats. Results : In vitro, CS didn't effect levels of lipid peroxide. the activities, and the ratio of type conversion of xanthine oxidase. In the hydrocortisone acetate-treated rats, lipid peroxide, the activities, the ratio of type conversion of xanthine oxidase, and the death rate all increased. But, glutathione peroxidase and superoxide dismutase decreased. In vitro, after CS was administered to hydrocortisone acetatetreated rats, the levels of lipid peroxide in the liver, and the death rate decreased. However, the activities, and the ratio of type conversion of xanthine oxidase decreased. The body weight, glutathione peroxidase, and superoxide dismutase in+creased. The effects of Sinensis Cordyceps Broth did better than the effects of Sinensis Cordyceps Mycelia. Conclusions : These results suggest that CS decrease the activities of free radical generating enzymes which form lipid peroxide and increase the activities of oxygen free radical scavenging enzymes.

• Journal of Nutrition and Health
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• v.29 no.2
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• pp.159-165
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• 1996

This study was performed to investigate the effects of concentration and degree of unsaturation of fatty acids on cellular proliferation and lipid peroxide production, using primary skin fibroblasts from neonate rats Fibroblasts (CPD : 2.8-5.4). Cells were cultured either in control medium (Dulbecco's modified Eagle's medium supplement with 10% fetal bovine serum) or in media supplemented with various kinds (stearic, oleic, linoleic, arachidonic, linolenic, eicosapentaenoic acid) and amounts (5, 10, 25, 50, 100, 150uM)of fatty acids. Cellular proliferation ratio and lipid peroxice production were measured and morphological changes were observed. Cellular proliferation was inhibited and morphological changes were observed. Cellular proliferation was inhibited and morphological changes were observed in cells grown in stearic containing media. Oleic, arachidonic, and eicosapentaenoic aicd tend to stimulate cellualar proliferation, and linolenic acid had no effects. Lipid peroxide concentrations in fibroblasts increased in proportion to the contents and unsaturation of fatty acids in media. Especially supplementation of arachidonic acid accelerated cellualr lipid peroxidation. Free radicals may cause severs damage to biological molecules, so lipid peroxidation probably contributes cellular membrane damages. However there were little relationship between lipid peroxide production and cellular proliferation in this study. (Korean J Nutrition 29(2) : 159~165, 1996)