The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effects of the Extract of Samjasan on the Nitric Oxide Synthase Activity and the Level of Lipid Peroxide in Penis of Rats

삼자산(三子散)이 흰쥐 음경조직의 Nitric Oxide Synthase 활성 및 과산화지질 함량에 미치는 영향

  • We, Young-Taek (Department of Internal Medicine, College of Oriental Medicine, Dongguk Univ.) ;
  • Park, Jong-Hyuck (Department of Internal Medicine, College of Oriental Medicine, Dongguk Univ.) ;
  • Yoon, Cheol-Ho (Department of Internal Medicine, College of Oriental Medicine, Dongguk Univ.) ;
  • Jeong, Ji-Cheon (Department of Internal Medicine, College of Oriental Medicine, Dongguk Univ.) ;
  • Shin, Uk-Seob (Dept. of Pharmacy, Dongguk Medical Center) ;
  • Min, Gun-Woo (Department of Internal Medicine, College of Oriental Medicine, Dongguk Univ.)
  • 위영택 (동국대학교 한의과대학 내과학교실) ;
  • 박종혁 (동국대학교 한의과대학 내과학교실) ;
  • 윤철호 (동국대학교 한의과대학 내과학교실) ;
  • 정지천 (동국대학교 한의과대학 내과학교실) ;
  • 신억섭 (동국대학교 의료원 약제과) ;
  • 민건우 (동국대학교 한의과대학 내과학교실)
  • Published : 2001.03.30
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Abstract

Objectives : The following are the results of the experimental studies of Samjasan (SJS) extract on the nitric oxide synthase (NOS) activity and the level of lipid peroxide in the penises of rats. Methods : Cnidii Fructus, Cuscutae Semen, Schizandrae Fructus constitute SJS. 150 g of crushed crude drug was extracted with methyl alcohol, under reflux, for 24 hours, three times; the total extractive was evaporated under reduced pressure to give 28.6 g. Results : In vitro, the SJS extract didn't effect the activity of NOS. However, the SJS extract decreased the activities, the ratio of type conversion of xanthine oxidase, the levels of lipid peroxide, In vitro, after administration of the SJS extract to rats, the activities and ratio of type conversion of xanthine oxidase decreased, but the activity of NOS and the content of nitrite increased. Also, the levels of the superoxide anion radical and lipid peroxide decreased in the penises of rats. But, after administration of the SJS extract to rats, the levels of glutathione did not increase. The effects of the SJS extract did better as the dosage and the length of treatment increased. Conclusions : These results suggest that the SJS extract decreases the activities of free radical generating enzymes which form lipid peroxide and increases the NOS activity in the penises of rats. Therefore, the SJS extract is capable of improving of sexual ability in rats.

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References

  1. 대한한의학회지 v.17 no.2 발기부전 치료의 한의학적 접근 방법에 관한 연구 송봉근
  2. Urology v.28 no.3 Neuropathic factor as measured by peripheral motor nerve conduction. In:Diabetic secondary importence Palmer, J.D.K.;Fink, S.;Burger, R.H.
  3. Free radicals in chemistry and biology Milan, L.;Jozef, R.;Vilian, K.;Peter, P.;Ladislav, V.
  4. 남성성기능장애의 진단과 치료 김세철
  5. Nature v.327 Nitric oxide release accounts for the Obiologic activity of endothelial-derived relaxant factor Palmer, R.M.J.;Ferrige, A.G.;Moncada, S.
  6. Ph.D.thesis, Yeungnam Univ. Role of nitric oxide in penile erection Chung, H.C.
  7. 혜화의학 v.5 no.1 陽위에 대한 文獻的 考察 최훈섭;김철중
  8. 東醫腎系學 두호경
  9. 實用中醫腎病學 徐平(外)
  10. 한방성인병학회지 v.4 no.1 胡蘆巴 抽出液이 발기부전 개선효과에 관한 실험적 연구 김광진
  11. 대한한의학회지 v.19 no.2 楮實子 抽出液이 음경해면체의 Nitric oxide synthase 활성 및 Nitrite 함량에 미치는 영향 강정준;정지천;신억섭
  12. 대한한방내과학회지 v.21 no.3 冬蟲夏草가 Hydrocortisone을 투여한 흰쥐의 Nitric Oxide Synthase 활성 및 Testosterone 함량에 미치는 영향 민건우;박종혁;윤철호;정지천;신억섭;한영환
  13. 대한한방내과학회지 v.19 no.1 金櫻子 추출물이 음경해면체의 Nitric oxide synthase 활성 및 항산화효과에 미치는 영향 김경동;정지천
  14. 男性性功能障碍治療大全 王敬;杜傑慧(編著)
  15. J. Biol. Chem. v.244 The regulation of rat liver xanthine oxidas : Conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O) Stirpe, F.;Della, Corte E.
  16. J. Pharmacol. v.252 Comparison of spectrophoto metric and biological assay for nitric oxide and endothelium-derived relaxing factor (EDRF) : Neurospecificity of the diazotiazation reaction for NO and failure to detect EDRF Tracy, W.R.;Linden, J.;Michael, J.P.;Roger, A.J.
  17. Anal. Biochem. v.95 Assay for lipid peroxide in animal tissue by thiobarbituric acid reaction Ohkawa, H.;Ohishi, N.;Yaki, K.
  18. Biochem. Biophys. Res. Comm. v.65 The use of acetylated ferricytochrome c for the detection of superoxide radicals produced in biological membranes Azzi, A.;Montecucco, C.;Richter, C.
  19. Arch. Biochem. Biophys. v.82 Tissue sulfhydryl group Ellman, G.L.
  20. J. Biol. Chem. v.193 Protein measurement with folin phenol reagent Lowry, O.H.;Roserbrough, N.J.;Farr, A.L.;Randall, R.J.
  21. Free radicals, aging and degenerative disease Larry, W.O.;Terry, B.O.
  22. 漢藥臨床應用 이상인;안덕균;신민교
  23. J. Biol. Chem. v.267 no.16 Nitric oxide synthase regulatory sites Bredt, D.S.;Ferris, C.D.;Snyder, S.H.
  24. Nature v.347 Localization of nitric oxide synthase indicating a neural role for nitric oxide Bredt, D.S.;Hwang, P.M.;Snyder, S.H.
  25. J. Pharm. Pharmacol. v.41 Mechanistic toxicology : A radical perspective David, R.
  26. Am. J. Physiol. v.255 Role of xanthine oxidase and granulocytes in ischemia-reperfusion injury Granger, D.N.
  27. J. Biol. Chem. v.245 A mechanism for the production of ethylene from methional : The generation of the hydroxyl radical by xanthine oxidase Beauchamp, C.;Fridovich, I.
  28. N. Engl.: J. Med. v.312 no.3 Oxygen-derived free radicals in postischemic tissue injury McCord, J.M.
  29. FASEB. J. v.1 Oxidants and human disease : Some new concepts Barry, H.
  30. Pharmacol. Ther. v.37 no.2 Glutathione, free radicals and chemotherapeutic agents : Mechanism of free radical induced toxicity and glutathione dependent protection Ross, D.
  31. Pharmacol. Rev. v.36 no.2 Glutathione depletion and susceptibility Reed, D.J.;Farris, M.W.