• Journal of Microbiology and Biotechnology
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• 제25권2호
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• pp.238-246
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• 2015

A unicellular red microalga was isolated from environmental freshwater in Korea, and its morphological, molecular, and biochemical properties were characterized. Morphological analysis revealed that the isolate was a unicellular biflagellated green microalga that formed a non-motile, thick-walled palmelloid or red aplanospore. To determine the taxonomical position of the isolate, its 18S rRNA and rbcL genes were sequenced and phylogenetic analysis was performed. We found that the isolate was clustered together with other related Haematococcus strains showing differences in the rbcL gene. Therefore, the isolated microalga was classified into the genus Haematococcus, and finally designated Haematococcus sp. KORDI03. The microalga could be cultivated in various culture media under a broad range of pH and temperature conditions. Compositions of the microalgal cellular components were analyzed, and its protein, carbohydrate, and lipid compositions were estimated to be 21.1 ± 0.2%, 48.8 ± 1.8%, and 22.2 ± 0.9%, respectively. In addition, D-glucose and D-mannose were the dominant monosaccharides in the isolate, and its amino acids were composed mainly of aspartic acid, glutamic acid, alanine, and leucine. Moreover, several polyunsaturated fatty acids accounted for about 80% of the total fatty acids in Haematococcus sp. KORDI03, and the astaxanthin content in the red aplanospores was estimated to be 1.8% of the dry cell weight. To the best of our knowledge, this is the first report of an Haematococcus sp. isolated from Korea, which may be used for bioresource production in the microalgal industry.

• Nutrition Research and Practice
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• 제6권6호
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• pp.520-526
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• 2012

Various forms of fermented soybean products are well documented for their health benefits. The efficacy of anti-obesogenic effect of Doenjang, one of the most commonly used seasonings in Korean cuisine, has been reported only in animal models; thus, an evaluation of Doenjang needs to be conducted in human studies. We aimed to test the hypothesis that Doenjang supplementation reduces body weight and changes body composition in overweight adults. A total of 51 overweight adults participated in this study. A group of males with BMI ${\geq}23kg/m^2$ and waist to hip ratio (WHR) ${\geq}$ 0.90, and a group of females with BMI ${\geq}23kg/m^2$ and WHR ${\geq}$ 0.85 were randomly assigned to either a Doenjang supplement (9.9 g dry/day) group or a placebo group for a 12-week randomized, double-blind and placebo-controlled study. Anthropometric parameters, abdominal fat distribution by computerized tomography (CT) and blood components were measured before and after the intervention period. After the 12-week study, the Doenjang supplementation group had significant reductions in body weight (kg), body fat mass (kg) and body fat (%) compared to the placebo group, the supplementation of Doenjang resulted in a significant reduction in visceral fat ($cm^2$), although no changes were observed in total and subcutaneous fat are as ($cm^2$), serum lipid profiles and dietary intakes. The present study demonstrated that daily supplementation of 9.9 g dry/day of Doenjang for 12 weeks reduces body weight and visceral fat in overweight adults.

• 한국식품영양과학회지
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• 제37권8호
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• pp.985-991
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• 2008

키토산용액으로 발아시킨 키토산발아현미는 증류수만으로 발아시킨 일반발아현미보다 식이섬유, 총 페놀성 화합물, GABA, 그리고 phytic acid의 함량이 높게 나타났다. 또한, 체내 지방 흡수에 관여하는 lipase 저해 검정에서는 키토산 발아현미가 가장 높은 활성을 나타냈으며 일반발아현미도 현미에 비해 높은 활성을 나타냈다. 고지방 식이에 의한 흰쥐의 비만 억제 실험에서는 키토산발아현미군이 대조구와 일반발아현미에 비해 체중 및 복부지방량이 낮고 분변 중의 조지방 함량은 높은 경향을 보였다. 또한 혈중 총콜레중성지질, 및 LDL-C의 농도는 낮은 반면 HDL-C 농도는 높은 경향을 나타냈다. 발아현미 제조에 있어 키토산 처리는 발아하는 동안 비만 억제 및 콜레스테롤 감소에 효과가 있는 기능성 성분을 증대시키는 유용한 방법으로 사료된다.

• 한국식품영양과학회지
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• 제33권1호
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• pp.132-139
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• 2004

본 연구는 일반 시판된장과 녹차된장의 일반성분, 유리아미노산, 지방산, 유기산의 함량과 항산화 활성도를 비교하였으며, 그 결과는 다음과 같다. 수분함량은 일반된장에 비해 녹차된장이 높게 나타났으며, 조 단백 함량은 일반된장에 비해 녹차된장이 낮게 나타났다. 조 지방 함량의 경우 일반된장과 녹차된장이 비슷하게 나타났으며, 회분함량은 녹차된장이 일반된장의 평균값을 나타냈고, 당질 함량은 녹차된장이 가장 낮게 나타났다. 섬유소함량은 일반 시판 된장 중 H제품을 제외하고는 비슷하게 나타났으며, 열량 역시 녹차된장이 가장 낮았다. 녹차된장의 색도는 일반된장에 비해 비교적 낮게 나타났다. 녹차된장중의 총 카테킨 함량은 2.3%로 나타났다. 유리 아미노산의 경우 시판된장 중 C사 제품은 sarcosine이 337.8 mg%로 가장 많은 함량을 나타냈고, 다음으로 proline(268.4 mg%), glutamic acid(268.0 mg%)순으로 나타났으며, H사 제품은 glutamic acid가 831.6 mg%로 가장 높았고, leucine(491.0 mg%), phenylalanine(364.3 mg%)순이었고, S사 제품은 역시 glutamic acid(855.2 mg%)가 시료중에서 가장 높게 나타났으며, 다음으로 alanine(567.9 mg%), phenylalanine(413.9 mg%), valine(400.3 mg%) 순이었다. 녹차된장 역시 glutamic acid(326.24 mg%)가 가장 많이 함유되었고, 다음으로 proline(320.9 mg%), leucine(221.49 mg%)순으로 나타났다. 포화지방산은 녹차된장이 일반된장에 비해 약간 높았으며, 불포화지방산중 단일 불포화지방산은 일반된장에 비해 녹차된장이 약간 낮았고, 다 불포화지방산은 녹차된장이 일반된장의 가장 낮은 값과 비슷하게 나타났다. 총 지방산중에 linoleic acid(51%)가 가장 높게 나타났으며, 다음으로 oleic acid(21∼24%), palmitic acid(10∼11%), stearic acid(3∼4%) 순으로 나타났다 된장의 유기산은 주된 유기산이 oxalic acid였으며, lactic, succinic acid를 제외하고는 대체로 일반 시판된장이 녹차된장에 비해 1.6∼2.7배 높게 나타났다. 녹차된장의 항산화 활성도는 타 일반된장에 비하여 높게 나타났다. 본 연구결과 기능성을 가진 녹차된장이 일반시판된장에 비해 열량이 낮으며, 항산화 활성도가 높으므로 장기간 섭취시 비만예방 및 노화방지에 효과가 있을 것으로 사료된다.

• 동의생리병리학회지
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• 제23권5호
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• pp.1199-1205
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• 2009

The purpose of this case-control study was done to examine the relationship among the cerebral infarction, blood lipids and homocysteine. We compared the components of blood lipids and homocysteine between cerebral infarction patients group (n=127) and controls group (n=158). We performed Pearson's chi-square test and Student's t-test for univariate analysis, Binary logistic regression for multivariate analysis to evaluate risk factors of cerebral infarction and Pearson's correlation analysis to investigate correlation between blood lipids and homocysteine. The results were as follows. The blood levels of High density lipoprotein cholesterol(HDL-Chol) and Low density lipoprotein cholesterol (LDL-Chol) were significantly lower in patients group, while age, the blood levels of Triglyceride(TG) and homocysteine were significantly higher in patients group. Hypertension had a 4.62 odds ratio, $TG{\geq}150\;mg/d{\ell}$ had a 2.33 odds ratio, HDL-Chol ${\leq}40\;mg/d{\ell}$ had a 6.85 odds ratio, but sex, DM, T-Chol, LDL-Chol had no direct relationship with odds ratio(non significant). In addition, among T-Chol and TG , HDL-Chol, LDL-Chol, they had positive correlation each other. Between TG and HDL-Chol had negative correlation each other. Homocysteine was not correlated with blood lipids adjusted for age and sex. These results suggest that low HDL-Chol and high TG may be risk factor of cerebral infarction. The correlation between homocysteine and blood lipids was not proven.y urther reserch on the subject is needed.

• 동의생리병리학회지
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• 제23권2호
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• pp.351-359
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• 2009

Etiological studies of diabetes and its complications showed that oxidative stress might play a major role, Therefore, many efforts have been tried to regulate free oxygen radicals for treating diabetes and its complications. Mori Fructus extract has been known to be effective for the antidiabetic, antihyperlipidemic and antiobesitic prescription, and composed of four crude herbs, The aim of this study was to investigate the effect of Mori Fructus extract in male ob/ob mouse with severe obesity, hyperinsulinemia, hyperglycemia, hyperlipidemia. Mice were grouped and treated for 5 weeks as follows. Both the lean (C57BL/6J black mice) and diabetic (ob/ob mice) control groups received standard chow. The experimental groups were fed with a diet of chow supplemented with 7.5, 15 and 30 mg Mori Fructus extract per 1 kg of body weight for 14 days. The effects of Mori Fructus extract on the ob/ob mice were observed by measuring the serum levels of glucose, insulin, lipid components, and the kidney levels of reactive oxygen species (ROS), MDA+HAE, GSH and also the enzyme activities involved in polyol pathway. Western blotting was performed using anti-AGE, anti-RAGE respectively. Mori Fructus extract lowered the levels of serum glucose and insulin in a dose dependent manner. Total cholesterol, triglyceride and free fatty acid levels were decreased, while the HDL-cholesterol level was increased, in Mori Fructus extract treated groups. Renal aldose reductase and sorbitol dehydrogenase activities were increased in the ob/ob mice, whereas those were inhibited in the Mori Fructus extract-administered groups. Mori Fructus extract inhibited the generation of ROS in the kidney. MDA+HAE level was increased and the GSH level was decreased in the ob/ob mice, whereas those were improved in the Mori Fructus extract-administered groups. Mori Fructus extract inhibited the expression of AGE, RAGE in the ob/ob mice. The results suggested that Mori Fructus exerted the antidiabetic and antihyperlipidemic activities by regulating the activities of polyol pathway enzymes, scavenging the ROS, decreasing the MDA+HAE level, increasing the GSH level and inhibiting the expression of AGE, RAGE in the ob/ob mice.

• 한국식품영양학회지
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• 제22권3호
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• pp.357-366
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• 2009

The principal objective of this study was to evaluate the association between plasma antioxidant levels and metabolic syndrome in male workers, and to provide basic information regarding the control and prevention of metabolic syndrome. We analyzed 163 male workers who had participated in annual medical examinations from January to December 2007. The subjects were classified into normal and metabolic syndrome groups according to the NCEP-ATP III criteria and the Asia-Pacific criteria for waist circumference. Anthropometric parameters, lifestyles, blood lipid profiles, and antioxidant levels were evaluated. As compared to the normal group, the metabolic syndrome group evidenced significantly higher plasma levels of $\alpha$- tocopherol(p<0.05) and retinol(p<0.05), but significantly lower plasma levels of lycopene(p<0.05) and $\beta$-carotene(p<0.05). This tendency was found to be predominantly attributable to increases in the number of metabolic syndrome components. In our simple regression analysis, higher plasma levels of $\alpha$-tocopherol($\beta$=0.001, p<0.01) and retinol($\beta$=0.021, p<0.001) were associated with significantly higher risks of metabolic syndrome, but lycopene($\beta$=-1.499, p<0.01) and $\beta$-carotene($\beta$=-0.048, p<0.01) were associated with significantly lower risks of metabolic syndrome. Retinol($\beta$=0.013, p<0.05) and $\beta$-carotene($beta$=-0.044, p<0.01) were associated significantly with metabolic syndrome, when adjusted for age and BMI. These data indicate that the plasma levels of $\alpha$-tocopherol, retinol, lycopene, and $\beta$-carotene are associated with metabolic syndrome. Specifically, low lycopene and $\beta$-carotene levels in the plasma appear to increase the risk of metabolic syndrome. Therefore, proper nutritional education programs for male workers are required to increase dietary intakes of antioxidant vitamins. Further studies will be necessary to determine whether antioxidant levels can be utilized as a predictive or a preventive factor.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.262-262
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• 2017

Brown rice contains a variety of nutritional and bio-functional components compared to milled rice. However, low consumer preference for steamed brown rice and short shelf life has been implicated as deterrents to the direct consumption of brown rice. This study was undertaken to determine the effect of degree of milling (DOM) on pasting and aging properties of brown rice cultivar. Short-grain (Japonica type) brown rice cv. Boseogchal (BSC), Baegjinju (BJJ), and Hiami (HIAM) were evaluated every month during 4 months storage. Rice cultivar were categorized into waxy (BSC), medium-waxy (BJJ), and non-waxy (HIAM) of which amylose contents were 6.81%, 10.06%, and 16.69~16.72%, respectively. Peak viscosity was the highest in BJJ followed by HIAM, and BSC, showing peak viscosity was lower at low temperature storage. At storage at room temperature from 60 to 120 days, the FFA content significantly increased in the rice samples with DOM1% followed by 3% and 5%, while not significant increase in DOM0% brown rice: at 120 days, the FFA ranged in 28.35~41.48, 111.99~130.06, 76.87~91.99, and 47.13~62.37 mg KOH/100g for DOM 0%, 1%, 3% and 5%, respectively. It indicated that the fat rancidity was the least in the order of DOM 0%, 5%, 3% and 1%. Storage at low temperature could reduce fat hydrolysis with lower FFA release, but rice grain with 1% MD also released more FFA compared to the other samples. It could be assumed that the most outer bran layer, approximately 1~3%, could provide a strong protection from fat rancidity. Pasting viscosity of rice samples also increased with increasing DOM rate. Results indicated that DOM was the most significant factor for lipid rancidity during storage, resulting in high FFA formation. It suggested that brown rice with DOM 0% and/or more than DOM 5% could provide the best shelf life.

• 한국토양비료학회지
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• 제25권2호
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• pp.138-142
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• 1992

들깨의 시비조건(施肥條件)에 따른 수량(收量)과 함유량(含油量) 등을 조사한 결과는 다음과 같다. 1. 시비조건(施肥條件)에 따라 초장(草長), 가지수, 마디수, 화방수는 별 차이가 없었으며 가리시용(加里施用)은 들깨의 수량(收量)과 함유량(含油量)을 증가시키는 효과가 있었고,인산혼용(燐酸混用)은 들깨의 수량(收量)을, 석회(石灰)는 함유랑(含油量)을 증가시키는 효과가 있었다. 2. 가리(加里), 석회(石灰), 인산(燐酸)의 조합시비(組合施肥)는 종실(種實)을 크게 하나 함유량(含油量)은 낮았다. 3 들깨의 지방산조성(脂肪酸組成)은 lenolenic acid 가 64.5%로 최고(最高)였으며 다음이 oleic acid (14.0%), linoleic acid(13.8%) palmitic acid (5.4%). stearic acid (1.8%) 순이었고 시비조건(施肥條件)에 따른 특별한 분포경향을 보이지 않았다. 4. 들깨 종실(種實)의 회분중 무기성분(無機成分)은 K(21.3%), Ca(12.8%), Mg(8.2%), Fe(0.34%), Zn(0.15 %), Mn(0.1%), Na(0.08%) 순이었다.

• 한국수산과학회지
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• 제45권4호
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• pp.307-316
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• 2012

This study evaluated the possible use of white muscle from cooked skipjack tuna as a constituent of diet foods. White muscles from the belly and dorsal area of cooked skipjack tuna were identified as anterior, median, and posterior. The skipjack tuna white muscle contained more moisture and ash (except for part I in both the belly and dorsal muscles) than chicken muscle, while it had less crude protein and crude lipid (except for part II in belly muscle). The yield was the highest in part I of both the dorsal and belly parts among the various parts of white muscles. The skipjack tuna white muscle contained 14-18% fewer calories than chicken breast muscle. Part I from both the belly and dorsal muscles had higher total amino acid contents than the other parts, but lower contents than chicken breast muscle. White muscle of skipjack tuna was rich in minerals, such as phosphorus, iron, and zinc. The total free amino acid content of part I in the belly and dorsal muscles was 1,152.1 and 1,215.7 mg/100 g, respectively, and was 1.7-1.8 times higher than in chicken breast muscle. The major amino acids in the white muscles from skipjack tuna were taurine, histidine, anserine, and carnosine. Based on these results, if it is possible to mask the fish odor, all parts of the white muscle from skipjack tuna could be used as constituents of diet foods.