• 한국수산과학회지
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• 제46권4호
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• pp.371-376
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• 2013

A feeding trial was conducted to determine the optimum dietary protein and lipid levels for the growth of juvenile muddy loach Misgurnus anguillicaudatus. Eight experimental diets (designated as P20L7, P20L14, P30L7, P30L14, P40L7, P40L14, P50L7 and P50L14) were formulated to contain 20%, 30%, 40% and 50% protein with 7% and 14% lipid. Three replicate groups of muddy loach (1.5 g/fish) were fed one of the experimental diets ad libitum for 8 weeks. Survival of fish fed the P20L14 diet was lower than in other groups (P<0.05). Specific growth rate (SGR) and feed efficiency (FE) of fish were affected by dietary protein (P<0.05) and lipid (P<0.005) levels. SGR and FE of fish tended to decrease with increasing dietary lipid level at the same protein level. There were no significant differences in SGR and FE among fish fed diets containing different protein levels ranging from 30% to 50% with the same lipid level. Whole body moisture and lipid contents were affected by dietary protein and lipid levels. Crude lipid content of fish fed the 14% lipid diets was higher than that of fish fed 7% lipid diets at each protein level. The results of this study indicate that a diet containing 30% protein with 7% lipid would be suitable for the optimum growth and effective feed utilization of juvenile muddy loach.

• 한국식품영양학회지
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• 제8권3호
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• pp.239-252
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• 1995

The results which were examined changes In contents of constitutive lipid and fatty acid during the natural drying of Pacific Saury were as follows. During the natural drying of Pacific Saury, offal lipid and moisture contents gradually decreased. In the survey of rancidity degree extracted lipid of Pacific Saury, peroxide value was gradually Increased, TBA and acid value were radically increased thereafter 9 days of drying. Neutral lipid, phospholipid and glycolipid contents of total lipid were 95%, 4% and 2%, respectively, and in the neutral lipid, content of TG was more than 90%, and in the phospholipid, contents of PC and PE were 45∼85%, 27∼34%, respectively. Fatty acid composition of total lipid was substantially higher in C18:0 and C20:1, C22:1, C22:6 contents and fatty acid composition of neutral lipid was higher In C22:1, C22:1, contents and fatty acid composition of phospholipid was the highest in C22:6 content. In the fatty acid composition of glycolipid, monounsaturated fatty acid content has substantially higher, C16:0 content was gradually Increased In saturated fatty acid and C22:1 content was gradually decreased in monounsaturated fatty acid. It showed that fatty acid composition of triglyceride has higher in monounsaturated fatty acid content and contents of C20:1, C22:1 was gradually decreased.

• BMB Reports
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• 제43권3호
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• pp.219-224
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• 2010

Lipid peroxidation is known to be an important factor in the pathologies of many diseases associated with oxidative stress. We assessed the lipid peroxidation induced by the reaction of ferritin with $H_2O_2$. When linoleic acid micelles or phosphatidyl choline liposomes were incubated with ferritin and $H_2O_2$, lipid peroxidation increased in the presence of ferritin and $H_2O_2$ in a concentration-dependent manner. The hydroxyl radical scavengers, azide and thiourea, prevented lipid peroxidation induced by the ferritin/$H_2O_2$ system. The iron specific chelator desferoxamine also prevented ferritin/$H_2O_2$ systemmediated lipid peroxidation. These results demonstrate the possible role of iron in ferritin/$H_2O_2$ system-mediated lipid peroxidation. Carnosine is involved in many cellular defense processes, including free radical detoxification. In this study, carnosine, homocarnosine, and anserine were shown to significantly prevent ferritin/$H_2O_2$ system-mediated lipid peroxidation and also inhibited the free radical-generation activity of ferritin. These results indicated that carnosine and related compounds may prevent ferritin/$H_2O_2$ system-mediated lipid peroxidation via free radical scavenging.

• Journal of Nutrition and Health
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• 제25권2호
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• pp.116-122
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• 1992

This study determined hepatic microsomal lipid peroxide values glucose 6-phosphatase NA-DPH-cytochrome P450 reductase and cytosolic glutathione S-transferase activites to examine the effects of methyl group deficiency on hepatic lipid peroxidation in rats treated with diethylni-trosamine(DEN) and 2-acetylamionfluorene(AAF) Weanling sprague Dawley male rats were fed the diet with methyl group supplemented or deficient. Two weeks after feeding rate were injected with a single of 200mg/kg body weight DEN intraperitoneally and after four weeks 0.02% AAF containing diets were fed for two weeks. Animals were sacrificed at 6th week. Microsomal lipid peroxide values were tended to increase in methyl group deficiency(MD). Especially in case of carcinogen tratments lipid peroxide values were increased significantly in MD. Microsomal glucose 6-phophatase activities were decreased by MD and carcinogens and in MD with carcinogen group (MD+C) the enzyme activites were the lowest Glucose 6-phosphatase activities were negatively correlated with lipid peroxidation. Microsomal NADPH-cytochrome P450 reductase activities were the highest in MD+C and correlated positively with lipid peroxidation. Cytosolic glutathione S-transferase activities were the highest in MD+C Methyl group deficiency induces lipid peroxidation especially in case of being exposed to carcinogens. Therefore the results suggest that lipid peroxidation may be one of the meachanisms of carcinogensis by methyl group deficiency.

• 분석과학
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• 제30권2호
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• pp.89-95
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• 2017

The thermodynamic functions for the binding of the peptide Substance P (SP) on the surface of lipid vesicles made of various types of lipids were obtained by using isothermal titration calorimetry. The reaction enthalpies measured from the experiments were -0.11 to $-4.5kcal\;mol^{-1}$. The sizes of the lipid vesicles were measured with dynamic light scattering instrument in order to get the correlation between the reaction enthalpies and the vesicle sizes. The bindings of SP on the lipid vesicles with diameter of 37 to 108 nm were classified into the enthalpy-driven reaction or the entropy-driven reaction according to the size of the lipid vesicles. For the enthalpy-driven binding reaction, the significance of the electrostatic interactions between SP and lipid molecules was affirmed from the experimental results of the DMPC/DMPG/DMPH and DMPC/DMPS/DMPH vesicles as well as the importance of the hydrophobic interactions between hydrophobic groups of SP and lipid molecules.

• 한국수산과학회지
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• 제36권2호
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• pp.113-119
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• 2003

A feeding trial was carried out to Investigate the effect of lipid level and herb mixture in the diets on growth of juvenile parrot fish, Oplegnathus fasciatus. Two plicate groups of fish averaging 4.5 g were fed four experimental diets containing herb mixture (0 and 5 g/kg diet) at each of two lipid levels $(8\%\;and\;16\%)$ for 8 months from summer to spring (water temperature, $7.2-25.4^{\circ}C)$. Weight gain improved with increasing dietary lipid level or supplementation of herb mixture during the first 4 months feeding period. Weight gain of fish fed the diet containing $16\%$ lipid level was significantly higher (P<0.05) than that of fish fed the diet containing $8\%$ lipid level. Weight gain of fish fed the diet with herb mixture was significantly higher (P<0.05) than that of fish fed the diet without herb at the $16\%$ lipid level. Feed efficiency was influenced by dietary lipid level or herb mixture during the first 4 months. On the other hand, weight gain and feed efficiency were not affected by dietary lipid level and herb mixture after 4 months until end of feeing period. Survival of fish fed the diet containing $8\%$ lipid without supplementation of herb mixture was the lowest among the groups (P<0.05). Lipid contents of liver and viscera tended to increase with increasing dietary lipid level during the first 4 months feeding period, however muscle lipid content was not influenced by dietary lipid and herb. Total cholesterol of serum was influenced by dietary lipid level during the first 4 months feeding period. These results indicate that an increase of dietary lipid level from $8\%\;to\;16\%$ and supplementation of herb mixture can improve growth performance of juvenile parrot fish when water temperature is optimum for growth such as the summer season in Korea.

• 한국식품위생안전성학회지
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• 제5권4호
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• pp.237-242
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• 1990

Streptozotocin에 의한 적혈구의 막 lipid peroxidation 측정시 TBA법의 적합성에 관하여 검토하기 위하여 Streptozotocin와 TBA의 직접반응 여부를 연구하였다. 여러 농도의 Streptozotocin 와 20% RBC suspension을 함께 배양한 후 lipid peroxidation을 TBA법으로 측정했을 때, Streptozotocin의 농도에 따라 흡광도가 증가하다가 100mM부터는 plateau를 이루었다. 그런데 적혈구를 제거한 대조실험에서도 발색단이 관찰되었다. 시험관에서 5mM의 Streptozotocin와 1% TBA를 산성조건에서 가열 직접 반응시킨 후 spectrum을 찍은 결과 TBA법 측정파장인 532nm에서 새로운 peak가 생겼다. 또 이 파장에서의 흡광도는 Streptozotocin 농도에 따라 증가하다가 25mM부터는 plateau가 되었다. 이러한 결과들은 적혈구와 Streptozotocin를 함께 배양하여 lipid peroxidation을 측정할 때, cell 내로 유입된 Streptozotocin 가 TBA와 직접반응을 할 수 있음을 제시한다. 그리고 다른 측정법인 lipid conjugation은 233nm에서의 흡수를 측정하는데. Streptozotocin 자체가 200-300nm에서 흡수를 나타내므로 이 방법으로도 lipid peroxidation 측정을 방해할 수 있다. 따라서 Streptozotocin에 의한 적혈구막의 lipid peroxidation은 TBA method 및 lipid conjugation 방법으로 측정하기는 부적합한 것으로 사료된다.

• 한국수정란이식학회지
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• 제26권1호
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• pp.71-78
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• 2011

This study was undertaken to find out the effect of cholesterol and serum albumin on sperm ability and lipid peroxidation levels period to the liquid storage of miniature pig sperm. Ejaculated semen from miniature pigs was collected by gloved-hand method into a pre-warmed ($37^{\circ}C$) thermos bottle, and extended with Modena solution {with and without BSA, methyl-beta-cyclodextrin (-cholesterol) and cholesterol loaded cyclodextrin (+cholesterol)}. Each semen was assessed for viability (SYBR-14/PI staining) and acrosome intactness, intensity and capacitation status by chlorotetracycline (CTC) staining at 1, 3, 5, 7 and 10 days of storage. At for the effects of cholesterol and serum albumin on lipid peroxidation, semen were incubated with $H_2O_2$ ($10\;{\mu}M$), and lipid peroxidation level were measured by flow cytometry using the lipid peroxidation reporter probe $C_{11}-BODIPY^{581/591}$. The result, lipid peroxidation level in sperm added with cholesterol were lower in $10\;{\mu}M$ $H_2O_2$ compared to the added sperm with serum albumin. Also, added cholesterol to sperm had significant (p<0.05) higher viability when storage for 7 and 10 days and lower when 10 days of storage percentage of acrosome-reacted sperm (AR pattern) in acrosome state as say result compared to other treated groups. In conclusion, role of cholesterol during lipid storage in miniature pig spermatozoa was protected boar spermatozoa from lipid peroxidation prior to lipid storage. Addition serum albumin during lipid storage in sperm may be induce sperm membrane damage by lipid peroxidation. Therefore, addition of cholesterol to miniature pig sperm will be lead to extension of liquid storage periods.

• Journal of Microbiology and Biotechnology
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• 제26권7호
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• pp.1290-1302
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• 2016

Microalgae are being researched for their potential as attractive biofuel feedstock, particularly for their lipid production. For maximizing biofuel production, it is necessary to explore the effects of environmental factors on algal lipid-producing potential. In this study, the effects of nitrogen (N) sources (NO₂-N, NO₃-N, urea-N, NH₄-N, and N-deficiency) and carbon-to-nitrogen ratios (C/N= 0, 1.0, 3.0, and 5.0) on algal lipid-producing potential of Chlorella sp. HQ were investigated. The results showed that for Chlorella growth and lipid accumulation potential, NO₂-N was the best amongst the nitrogen sources, and NO₃-N and urea-N also contributed to algal growth and lipid accumulation potential, but NH₄-N and N-deficiency instead caused inhibitory effects. Moreover, the results indicated that algal lipid-producing potential was related to C/N ratios. With NO₂-N treatment and carbon addition (C/N = 1.0, 3.0, and 5.0), total lipid yield was enhanced by 12.96-20.37%, but triacylglycerol (TAG) yields decreased by 25.52-94.31%. As for NO₃-N treatment, carbon addition led to a 17.82-57.43%/25.86-82.67% reduction of total lipid/TAG yields. When NH₄-N was used as the nitrogen source, total lipid/TAG yields were increased by 46.67-113.33%/28.99-74.76% with carbon addition. The total lipid/TAG yields of urea-N treatment varied with C/N ratios. Overall, the highest TAG yield (TAG yield: 38.75 ± 5.21 mg/l; TAG content: 44.16 ± 4.35%) was achieved under NO₂-N treatment without carbon addition (C/N = 0), the condition that had merit for biofuel production.

• 한국식생활문화학회지
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• 제13권5호
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• pp.519-528
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• 1998

This study was conducted to investigate the changes in lipid components of red pepper under various processing conditions such as drying, exposing to lights, extraction and storage conditions. The material used in this study were Chungyang cultivars which contained the largest amount of capsaicinoids and Dabok cultivars which is most popular among the various cultivars produced in Korea. Total lipid content of Chungyang was 15.7% and those of Dabok, Udeungsang and Hongilpum were about 17%. The neutral lipid content in free lipid of red pepper was $78.5{\sim}80.3%$ Phospholipid and glycolipid content in bound lipid were $53.8{\sim}56.9%$ and $35.3{\sim}38.7%$ respectively. The major fatty acids of lipid were linoleic, palmitic and oleic acid. Linoleic acid was presented mainly in pericarp, seed and placenta, whereas the most of palmitic acid was presented in stem. Lipid content of cut and whole red pepper were decreased $24.7{\sim}28%$ and $18.1{\sim}21.5%$ by drying for 48hrs at $65^{\circ}C$ and $95^{\circ}C$. And lipid content was also decreased $3.5{\sim}3.6%$ when the red pepper powder was exposed to 15,000 lux of incandescent light for 30 days at $40^{\circ}C$ and $4{\sim}4.9%$ to fluorescent light. The lipid was extracted the highest content of $50.5{\sim}51.7%$ by acid solution(pH2) and the lowest content by neutral solution, and the higher the salt concentration, the greater the lipid was extracted. During storage at $4^{\circ}C,\;25^{\circ}C,\;40^{\circ}C$, for 30 days the lipid content was not much changed but linoleic acid content was decreased during storage at the same conditions.