• Asian-Australasian Journal of Animal Sciences
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• v.28 no.7
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• pp.926-935
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• 2015

The efficiency of genome-wide association analysis (GWAS) depends on power of detection for quantitative trait loci (QTL) and precision for QTL mapping. In this study, three different strategies for GWAS were applied to detect QTL for carcass quality traits in the Korean cattle, Hanwoo; a linkage disequilibrium single locus regression method (LDRM), a combined linkage and linkage disequilibrium analysis (LDLA) and a $BayesC{\pi}$ approach. The phenotypes of 486 steers were collected for weaning weight (WWT), yearling weight (YWT), carcass weight (CWT), backfat thickness (BFT), longissimus dorsi muscle area, and marbling score (Marb). Also the genotype data for the steers and their sires were scored with the Illumina bovine 50K single nucleotide polymorphism (SNP) chips. For the two former GWAS methods, threshold values were set at false discovery rate <0.01 on a chromosome-wide level, while a cut-off threshold value was set in the latter model, such that the top five windows, each of which comprised 10 adjacent SNPs, were chosen with significant variation for the phenotype. Four major additive QTL from these three methods had high concordance found in 64.1 to 64.9Mb for Bos taurus autosome (BTA) 7 for WWT, 24.3 to 25.4Mb for BTA14 for CWT, 0.5 to 1.5Mb for BTA6 for BFT and 26.3 to 33.4Mb for BTA29 for BFT. Several candidate genes (i.e. glutamate receptor, ionotropic, ampa 1 [GRIA1], family with sequence similarity 110, member B [FAM110B], and thymocyte selection-associated high mobility group box [TOX]) may be identified close to these QTL. Our result suggests that the use of different linkage disequilibrium mapping approaches can provide more reliable chromosome regions to further pinpoint DNA makers or causative genes in these regions.

• Journal of Forest and Environmental Science
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• v.36 no.3
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• pp.243-254
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• 2020

This study was conducted to develop a forest education program through middle school curriculum linkage. We used the ADDIE model, comprising the five phases of Analysis, Design, Development, Implementation and Evaluation, to secure the objectivity of the program. In the Analysis phase, middle school textbooks were analyzed while considering middle school students' needs for curriculum linkage and the developmental stages of adolescents. The Design phase focused on promoting an understanding of curriculum subjects based on what was reviewed in the Analysis phase and concretized and organized a program that can be implemented in the forest, with a focus on science and physical education, based on the results of middle school students' needs. We also established the objectives and goals of curriculum linkage forest education, established the concept of the program, and selected the educational contents, teaching-learning methods, and evaluation methods. In the Development phase, we developed a 2-night, 3-day program linkage with the middle school curriculum and created a manual for instructors and a workbook for students. In the Implementation phase, we revised and supplemented the program through the first test operation with 24 students in their second year of middle school, after which, we carried out a six-session program for the 2-night, 3-day as the second test operation with 17 students in their second year of middle school. In the Evaluation phase, the program was evaluated by the students who participated in the second test operation using questionnaires on satisfaction and curriculum linkage understanding as well as rating scales for attitudes toward forests and environmental sensitivity. The results showed that middle school students showed positive significance in satisfaction and understanding of the middle school curriculum as well as attitudes toward forests and environmental sensitivity through the forest education program.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.12
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• pp.1672-1679
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• 2013

Linkage disequilibrium between markers or genetic variants underlying interesting traits affects many genomic methodologies. In many genomic methodologies, the effective population size ($N_e$) is important to assess the genetic diversity of animal populations. In this study, dairy cattle were genotyped using the Illumina BoviveHD Genotyping BeadChips for over 777,000 SNPs located across all autosomes, mitochondria and sex chromosomes, and 70,000 autosomal SNPs were selected randomly for the final analysis. We characterized more accurate linkage disequilibrium in a sample of 96 dairy cattle producing milk in Korea. Estimated linkage disequilibrium was relatively high between closely linked markers (>0.6 at 10 kb) and decreased with increasing distance. Using formulae that related the expected linkage disequilibrium to $N_e$, and assuming a constant actual population size, $N_e$ was estimated to be approximately 122 in this population. Historical $N_e$, calculated assuming linear population growth, was suggestive of a rapid increase $N_e$ over the past 10 generations, and increased slowly thereafter. Additionally, we corrected the genomic relationship structure per chromosome in calculating $r^2$ and estimated $N_e$. The observed $N_e$ based on $r^2$ corrected by genomics relationship structure can be rationalized using current knowledge of the history of the dairy cattle breeds producing milk in Korea.

• Journal of The Korean Society of Agricultural Engineers
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• v.57 no.5
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• pp.101-111
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• 2015

Establishment of appropriate data in certain formats is essential for agricultural water cycle analysis, which involves complex interactions and uncertainties such as climate change, social & economic change, and watershed environmental change. The main objective of this study was to develop web-based Data processing and Model linkage Techniques for Agricultural Water-Resource analysis (AWR-DMT). The developed techniques consisted of database development, data processing technique, and model linkage technique. The watershed of this study was the upper Cheongmi stream and Geunsam-Ri. The database was constructed using MS SQL with data code, watershed characteristics, reservoir information, weather station information, meteorological data, processed data, hydrological data, and paddy field information. The AWR-DMT was developed using Python. Processing technique generated probable rainfall data using non-stationary frequency analysis and evapotranspiration data. Model linkage technique built input data for agricultural watershed models, such as the TANK and Agricultural Watershed Supply (AWS). This study might be considered to contribute to the development of intelligent watercycle analysis by developing data processing and model linkage techniques for agricultural water-resource analysis.

• Genomics & Informatics
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• v.9 no.4
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• pp.143-151
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• 2011

In this study, we propose a novel, intuitive method of constructing an expression quantitative trait (eQT) network that is related to the metabolic syndrome using LOD scores and peak loci for selected eQTs, based on the concept of gene-gene interactions. We selected 49 eQTs that were related to insulin resistance. A variance component linkage analysis was performed to explore the expression loci of each of the eQTs. The linkage peak loci were investigated, and the "support zone" was defined within boundaries of an LOD score of 0.5 from the peak. If one gene was located within the "support zone" of the peak loci for the eQT of another gene, the relationship was considered as a potential "directed causal pathway" from the former to the latter gene. SNP markers under the linkage peaks or within the support zone were searched for in the database to identify the genes at the loci. Two groups of gene networks were formed separately around the genes IRS2 and UGCGL2. The findings indicated evidence of networks between genes that were related to the metabolic syndrome. The use of linkage analysis enabled the construction of directed causal networks. This methodology showed that characterizing and locating eQTs can provide an effective means of constructing a genetic network.

• Journal of The Korea Institute of Healthcare Architecture
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• v.23 no.4
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• pp.105-113
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• 2017

Purpose: While Japan undergoes super aged society, Japanese Elderly Facilities are trying to keep elderly residents from social isolation and expand regional interaction. Methods: This study analyzes regional linkage characteristics through 8 cases of Aichi-Elderly Facilities which realize regional interaction and symbiosis(life together) between various generations by visiting. Result: 1) Most researched facilities except SO provide and share opportunity for interaction with community and residents through space for regional interaction including cafe and various programs and interactive settings. 2) The facility which seeks regional linkage through Mixed-use of facilities can devide into (1) Mixed-use of facilities easy to found, (2) Addition of housing function (NM, SM, DM) and (3) Mixed-use with different facilities(HY, GM) 3) The characteristics of interaction and symbiosis between generations are (1) to adopt concept of life together to have interaction possibility with young generation and children: most studied facilities except SO (2) to establish elderly residence into facility to have interaction(FE, SM, GM) or place family room or single room into elderly residence (NM, DM, BN) (3) to disperse small facilities into community (FE) or facility or community realizing symbiosis through various facility arrangement (SM, GM). 4) Therefore, this study can categorize (1) Program network, (2) Temporary interaction, (3) Symbiosis residence, (4) Symbiosis community according to characteristics which regional linkage has. Implication: Regional Linkage is an important concept to improve social interaction in community-based facilities. It is a thought-provoking concept to Korean elderly facilities because Korean facilities are still far from a city and in isolated environment.

• Journal of Korean Medical Science
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• v.33 no.53
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• pp.343.1-343.8
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• 2018

Background: Linkage of public healthcare data is useful in stroke research because patients may visit different sectors of the health system before, during, and after stroke. Therefore, we aimed to establish high-quality big data on stroke in Korea by linking acute stroke registry and national health claim databases. Methods: Acute stroke patients (n = 65,311) with claim data suitable for linkage were included in the Clinical Research Center for Stroke (CRCS) registry during 2006-2014. We linked the CRCS registry with national health claim databases in the Health Insurance Review and Assessment Service (HIRA). Linkage was performed using 6 common variables: birth date, gender, provider identification, receiving year and number, and statement serial number in the benefit claim statement. For matched records, linkage accuracy was evaluated using differences between hospital visiting date in the CRCS registry and the commencement date for health insurance care in HIRA. Results: Of 65,311 CRCS cases, 64,634 were matched to HIRA cases (match rate, 99.0%). The proportion of true matches was 94.4% (n = 61,017) in the matched data. Among true matches (mean age 66.4 years; men 58.4%), the median National Institutes of Health Stroke Scale score was 3 (interquartile range 1-7). When comparing baseline characteristics between true matches and false matches, no substantial difference was observed for any variable. Conclusion: We could establish big data on stroke by linking CRCS registry and HIRA records, using claims data without personal identifiers. We plan to conduct national stroke research and improve stroke care using the linked big database.

• Korean Journal of Breeding Science
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• v.40 no.2
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• pp.97-100
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• 2008

Lectin protein and Kunitz trypsin inhibitor (KTI) protein of mature soybean seed are a main antinutritional factor in soybean seed. The Le gene controls a lectin protein and Ti gene controls the KTI protein in soybean. Ti locus has been located on linkage group 9 in the classical linkage map of soybean. Position of Le locus on linkage map was not identified. Genetic relationship between Ti locus and Le locus could be useful in soybean breeding program for the genetic elimination of these factors. The objective of this study was to determine the independent inheritance or linkage between Ti locus and Le locus in soybean seed. Two $F_2$ populations were developed from three parents (Gaechuck#1, T102, and PI548415). The $F_1$ seeds from Gaechuck#1 (titiLeLe) ${\times}$ T102 (TiTilele) and Gaechuck#1 (titiLeLe) ${\times}$ PI548415 (TiTilele) were obtained. The lectin and KTI protein were analysed from $F_2$ seeds harvested from the $F_1$ plants to find independent assortment or linkage between Ti locus and Le locus. The segregation ratios of 3 : 1 for Le locus (129 Le_ : 44 lele) and Ti locus (132 Ti_ : 41 titi) and were observed. The segregation ratios of 9 : 3 : 3 : 1 (95 Le_Li_ : 34 Le_titi: 37 leleTi_ : 7 leletiti) between Le gene and Ti gene in $F_2$ seeds were observed. This data showed that Ti gene was inherited independently with the Le gene in soybean. These results will be helpful in breeding program for selecting the line with lacking both KTI and lectin protein in soybean.

• Journal of Korean Society on Water Environment
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• v.35 no.1
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• pp.35-42
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• 2019

Urbanization has led to extreme changes in land use on urban watersheds. Most cities are becoming residential, commercial and industrial areas, making infiltration and storage of rainfall less favorable. The demand for LID (Low Impact Development) technology is increasing in order to mitigate this water cycle distortion and return to existing hydrological conditions. The LID technique is effective in reducing runoff by permeating the urban impervious area. However, considering the limit of the installation area and the financial requirement of the installation, there is not much research on the linkage of each LID component technology for optimum efficiency according to the appropriate scale. In this study, the effects of the LID facilities applied to the target site were simulated using the SWMM model, suggesting the optimal linkage method considering interconnectivity, and applying the effects as an existing installation of individual facilities. The water balance at the time of application of the LID technology, short-term and long-term rainfall event were compared. Also, the individual application and the linkage application were compared with each other. If each component technology has sufficient processing size, then linkage application is more effective than individual application.

• Journal of Korean Library and Information Science Society
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• v.55 no.2
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• pp.243-266
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• 2024

In foreign countries, ISNI-based creative work identification and linkage services have been established and operated in various fields, while in Korea, ISNI utilization and linkage services limited to some fields are operated mainly by the National Library of Korea. The purpose of this study is to propose an ISNI-based linkage and utilization service that can be applied in Korea. To achieve the purpose of this study, we examined overseas ISNI linkage cases and identified the requirements of related organizations. We also proposed four ways to build ISNI sharing and convergence services. These include establishing Identifiers Co-operation Initiatives (ICI), establishing creator profiles, activating ISNI through the publishing industry, and activating ISNI services through external linkages. We hope that this study will serve as a basis for building ISNI-based linkage and utilization services.